Agricultural Sciences in China最新文献

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells. 用于细胞超快速磁化的阳离子化磁铁素的合成。

IF 1.2

Agricultural Sciences in China Pub Date : 2016-12-13 DOI: 10.3791/54785

Sara Correia Carreira, James P K Armstrong, Mitsuhiro Okuda, Annela M Seddon, Adam W Perriman, Walther Schwarzacher

引用次数: 0

Resistance Level and Metabolism of Barnyard-Grass (Echinochloa crusgalli (L.) Beauv.) Populations to Quizalofop-p-ethyl in Heilongjiang Province, China 稗草(Echinochloa crusgalli, L.)抗性水平与代谢测定)。标题在黑龙江省的Quizalofop-p-ethyl的种群

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60192-2

Zhi-bo HUAN , Hong-jun ZHANG , Zhen HOU , Shao-yi ZHANG , Yang ZHANG , Wei-tang LIU , Ya-ling BI , Jin-xin WANG

引用次数: 23

Molecular Cloning and Characterization of Carnation EBF1 Gene During Flower Senescence and upon Ethylene Exposure and Sugar 香石竹EBF1基因在花衰老及乙烯和糖胁迫下的克隆与特性研究

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60187-9

Zhao-di FU , Hui-nan WANG , Juan-xu LIU , Hong-xue ZENG , Jiao ZHANG , Xiao-cong KUANG , Yi-xun YU

引用次数: 5

Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum 钙依赖性蛋白激酶基因NtCDPK12的克隆及其在高盐和干旱条件下的诱导表达

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60185-5

Shuai CHEN , Guan-shan LIU , Yuan-ying WANG , Yu-he SUN , Jia CHEN

引用次数: 6

Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-lnositol, Vitexin, and Isovitexin Contents in Mung Bean 近红外反射光谱法测定绿豆中d -丙烯醇、牡荆素和异牡荆素含量

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60200-9

Yang YAO, Xu-zhen CHENG, Gui-xing REN

{"title":"Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-lnositol, Vitexin, and Isovitexin Contents in Mung Bean","authors":"Yang YAO, Xu-zhen CHENG, Gui-xing REN","doi":"10.1016/S1671-2927(11)60200-9","DOIUrl":"https://doi.org/10.1016/S1671-2927(11)60200-9","url":null,"abstract":"<div><h3>Abstract</h3><p>Mung bean (<em>Vigna radiata</em> L.) is rich in D-<em>chiro</em>-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (<em>R</em><sup>2</sup>=0.90 for DCI, <em>R</em><sup>2</sup>=0.81 for vitexin, and <em>R</em><sup>2</sup>=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1986-1991"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60200-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91988854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat 小麦抗叶锈病基因Lr24的鉴定与分子标记

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60190-9

Na ZHANG, Wen-xiang YANG, Da-qun LIU

{"title":"Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat","authors":"Na ZHANG, Wen-xiang YANG, Da-qun LIU","doi":"10.1016/S1671-2927(11)60190-9","DOIUrl":"10.1016/S1671-2927(11)60190-9","url":null,"abstract":"<div><h3>Abstract</h3><p>This research was aimed to develop AFLP markers co-segregated with gene <em>Lr24</em> and validate the using for marker assisted selection (MAS). An F<sub>2</sub> population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the <em>Puccinia triticina</em> races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, <em>P-AGA/M-CTT<sub>289 bp</sub>, P-AGC/M-CAC<sub>188 bp</sub>, P-AGC/M-CAC<sub>162 bp</sub></em> and <em>P-ACG/M-CGC<sub>239 bp</sub></em> were co-segregated with <em>Lr24.</em> The AFLP fragment from the primer combination P-ACG/M-CGC was cloned, sequenced and converted into a STS marker named as <em>ASTS212.</em> Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker <em>SCS1302<sub>607</sub></em> developed by Gupta. 5R615, 5R616, 1R13, and 1R17 were identified and validated to contain gene <em>Lr24.</em> The marker is dominant and may be useful in identification the resistance gene <em>Lr24</em> in wheat and wheat breeding programs.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1898-1905"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60190-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Creatine Pyruvate Enhances Lipolysis and Protein Synthesis in Broiler Chicken 丙酮酸肌酸促进肉仔鸡脂肪分解和蛋白质合成

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60199-5

Juan CHEN , Hai-tian MA , Man WANG, Yi-li KONG, Si-xiang ZOU

{"title":"Creatine Pyruvate Enhances Lipolysis and Protein Synthesis in Broiler Chicken","authors":"Juan CHEN , Hai-tian MA , Man WANG, Yi-li KONG, Si-xiang ZOU","doi":"10.1016/S1671-2927(11)60199-5","DOIUrl":"10.1016/S1671-2927(11)60199-5","url":null,"abstract":"<div><h3>Abstract</h3><p>To assess the effects of creatine pyruvate (Cr-Pyr) on lipid and protein metabolism in broiler chickens, a total of 400 1-day-old male birds (Aconred) were randomly allocated to four groups, with each group replicating four times and each replicate involving 25 birds. The broilers were provided with a commercial diet supplemented with Cr-Pyr at 0, 1, 5, or 10% of the diet, respectively, for a period of 3 wk <em>ad libitum</em> (from 22 to 42 d). In the present study, body weight (BW) and average daily gain (ADG) of broilers decreased in 10% Cr-Pyr group (<em>P</em><0.01), whereas the relative leg and pectoral muscle weights were significantly higher than they were in the control group <em>(P</em><0.05). 5 or 10% Cr-Pyr of diets decreased the abdominal fat rate (AFR, abdominal fat/live weight) of the broilers. The serum or hepatic triglyceride (TG) concentrations were significantly lower in the 5 and 10% groups (P<0.01). In contrast, Cr-Pyr caused a marked increase in the serum non-esterified fatty acid (NEFA), high-density lipoprotein cholesterol (HDL-C) and creatine kinase (CK) concentrations (P<0.01). Supplementation with Cr-Pyr (5 and 10%) in the diet also increased glucagons (GLU), insulin (INS) or leptin (LEP) contents (P<0.01). The expression of hepatic peroxisomal proliferators-activated receptor a (PPAR-a) and carnitine palmitoyl transferase-I (CPT-I), muscle insulin-like growth factor I (IGF-I) were significantly elevated and myostatin mRNA level was reduced in the 5 and 10% groups <em>(P</em><0.05). It was found that supplementation with 5% Cr-Pyr improves both lipid and protein metabolism by regulating various metabolic parameters of broilers, while not adversely affects growth performance in broiler chickens.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1977-1985"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60199-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol,Vitexin,and Isovitexin Contents in Mung Bean 近红外反射光谱法测定绿豆中d -手性肌醇、牡荆素和异牡荆素含量

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60200-9

Yang Yao, Xuzhen Cheng, G. Ren

引用次数: 5

Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.) 水稻4号染色体每穗粒数基因(Gnp4)的精细定位与克隆

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60182-X

Zhan-ying ZHANG , Jin-jie LI , Guo-xin YAO , Hong-liang ZHANG, Hui-jing DOU, Hong-li SHI, Xing-ming SUN, Zi-chao LI

{"title":"Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)","authors":"Zhan-ying ZHANG , Jin-jie LI , Guo-xin YAO , Hong-liang ZHANG, Hui-jing DOU, Hong-li SHI, Xing-ming SUN, Zi-chao LI","doi":"10.1016/S1671-2927(11)60182-X","DOIUrl":"10.1016/S1671-2927(11)60182-X","url":null,"abstract":"<div><h3>Abstract</h3><p>Grain number per-panicle is one of the most important components for rice yield. Spikelets on the primary and secondary branches determine the grain number per-panicle in rice. In this study, we identified a natural mutant, <em>gnp4,</em> lack of lateral spikelet on the secondary branches in the field condition. In addition, the <em>Gnp4</em> and <em>Lax1-1</em> double mutant showed dramatically reduced secondary branches and spikelets in panicle at reproductive stage, and tillers at vegetative stage. By map-based cloning approach, and using four F<sub>2</sub> segregating populations, the <em>Gnp4</em> gene was finally mapped to a 10.7-kb region on the long arm of chromosome 4 in rice. In this region, only one gene was predicted, and genomic DNA sequencing of the 10.7-kb region showed no nucleotide differences between the mutant and wild type. Interestingly, we found that the methylation level of several cytosines in the promoter CpG islands region of the predicted gene in <em>gnp4</em> were different from the wild type. Thus, we propose that the DNA methylation changes at these sites may induce to decrease expression level of <em>Gnp4,</em> consequently, resulting in phenotypic variation.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1825-1833"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60182-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.) 大豆(Glycine max L. Merr.)新基因GmMF1的克隆与鉴定

Agricultural Sciences in China Pub Date : 2011-12-01 DOI: 10.1016/S1671-2927(11)60183-1

Wei JIANG, Shou-ping YANG, De-yue YU, Jun-yi GAI

{"title":"Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)","authors":"Wei JIANG, Shou-ping YANG, De-yue YU, Jun-yi GAI","doi":"10.1016/S1671-2927(11)60183-1","DOIUrl":"10.1016/S1671-2927(11)60183-1","url":null,"abstract":"<div><h3>Abstract</h3><p>Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS1A and its maintainer line NJCMS1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, <em>GmMF-T4A15,</em> was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA full-length sequence of 1 311 bp was obtained and named <em>GmMF1.</em> The expression characteristics of <em>GmMF1</em> were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that <em>GmMF1</em> was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by <em>GmMF1</em> is localized in the nucleus.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1834-1841"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60183-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3