Shuai CHEN , Guan-shan LIU , Yuan-ying WANG , Yu-he SUN , Jia CHEN
{"title":"钙依赖性蛋白激酶基因NtCDPK12的克隆及其在高盐和干旱条件下的诱导表达","authors":"Shuai CHEN , Guan-shan LIU , Yuan-ying WANG , Yu-he SUN , Jia CHEN","doi":"10.1016/S1671-2927(11)60185-5","DOIUrl":null,"url":null,"abstract":"<div><h3>Abstract</h3><p>Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca<sup>2+</sup> signal transduction. A full-length CDPK gene, <em>NtCDPK12</em> (GenBank accession number GQ337420), was isolated from common tobacco <em>(Nicotiana tabacum)</em> leaves by rapid amplification of cDNA ends (RACE). The <em>NtCDPK12</em> cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that <em>NtCDPK12</em> was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that <em>NtCDPK12</em> was induced by high-salt and drought stresses.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1851-1860"},"PeriodicalIF":0.0000,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60185-5","citationCount":"6","resultStr":"{\"title\":\"Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum\",\"authors\":\"Shuai CHEN , Guan-shan LIU , Yuan-ying WANG , Yu-he SUN , Jia CHEN\",\"doi\":\"10.1016/S1671-2927(11)60185-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Abstract</h3><p>Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca<sup>2+</sup> signal transduction. A full-length CDPK gene, <em>NtCDPK12</em> (GenBank accession number GQ337420), was isolated from common tobacco <em>(Nicotiana tabacum)</em> leaves by rapid amplification of cDNA ends (RACE). The <em>NtCDPK12</em> cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that <em>NtCDPK12</em> was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that <em>NtCDPK12</em> was induced by high-salt and drought stresses.</p></div>\",\"PeriodicalId\":7475,\"journal\":{\"name\":\"Agricultural Sciences in China\",\"volume\":\"10 12\",\"pages\":\"Pages 1851-1860\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60185-5\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Sciences in China\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1671292711601855\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601855","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum
Abstract
Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.
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