Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-lnositol, Vitexin, and Isovitexin Contents in Mung Bean

Yang YAO, Xu-zhen CHENG, Gui-xing REN
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引用次数: 5

Abstract

Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.

近红外反射光谱法测定绿豆中d -丙烯醇、牡荆素和异牡荆素含量
摘要绿豆(Vigna radiata L.)富含d-氨基肌醇(DCI)、牡荆素和异牡荆素,具有抗糖尿病和抑制晚期糖基化终产物形成的作用。采用近红外反射光谱法(NIRS)对绿豆中DCI、牡荆素和异牡荆素的含量进行了预测。光谱数据与高效液相色谱法(HPLC)线性化。利用偏最小二乘(PLS)算法建立了绿豆DCI、牡荆素和异牡荆素含量的预测模型。交叉验证程序表明,HPLC数据与NIRS预测具有良好的相关性(DCI R2=0.90,牡荆素R2=0.81,异牡荆素R2=0.90)。DCI、牡荆素和异牡荆素的预测含量分别为2.082 ~ 3.084%、1.277 ~ 1.307%和0.5998 ~ 0.6286%。结果表明,近红外光谱技术可用于绿豆中DCI、牡荆素和异牡荆素含量的快速检测。
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来源期刊
Agricultural Sciences in China
Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-
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3.2 months
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