Molecular Cloning and Characterization of Carnation EBF1 Gene During Flower Senescence and upon Ethylene Exposure and Sugar
引用次数: 5
Abstract
A cDNA clone encoding a putative EBF-like protein (DCEBF1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of 1 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBF1 expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.
香石竹EBF1基因在花衰老及乙烯和糖胁迫下的克隆与特性研究
摘要利用反转录PCR和cDNA末端快速扩增技术，从衰老石竹(Dianthus caryophyllus L.)花瓣总RNA中分离得到一个推测为ebf样蛋白(DCEBF1)的cDNA克隆。cDNA包含一个1878 bp的开放阅读框，对应625个氨基酸。Northern blot结果显示，内源和外源乙烯均能增强DCEBF1在花瓣和子房中的表达，而STS则能抑制其表达。损伤处理后，DCEBF1 mRNA积累量快速增加，与乙烯产量增加呈正相关。蔗糖处理后花瓣和子房中DCEBF1 mRNA表达水平均高于对照。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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