SUN Dong-bo, WU Rui, HE Xian-jing, WANG Shuang, LIN Yun-cheng, HAN Xu, WANG Yue-qiang, GUO Ting-ting, WU Guo-jun, YANG Ke-li
{"title":"奶牛子宫内膜炎金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌多重PCR诊断方法的建立","authors":"SUN Dong-bo, WU Rui, HE Xian-jing, WANG Shuang, LIN Yun-cheng, HAN Xu, WANG Yue-qiang, GUO Ting-ting, WU Guo-jun, YANG Ke-li","doi":"10.1016/S1671-2927(11)60160-0","DOIUrl":null,"url":null,"abstract":"<div><h3>Abstract</h3><p><em>Staphylococcus aureus, Escherichia coli</em> and <em>Bacillus cereus</em> are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of <em>S. aureus, E. coli</em> and <em>B. cereus,</em> and the detection limits were 10<sup>3</sup>, 10<sup>2</sup> and 10<sup>3</sup> CFU mL-<sup>1</sup>, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had <em>S. aureu</em> andii. <em>coli,</em> 9.39% had <em>E. coli</em> and <em>B. cereus,</em> and 9.71% had <em>S. aureus</em> and <em>B. cereus.</em> The rates of infections with <em>S. aureus, E. coli</em> and <em>B. cereuswere</em> 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1624-1629"},"PeriodicalIF":0.0000,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60160-0","citationCount":"19","resultStr":"{\"title\":\"Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis\",\"authors\":\"SUN Dong-bo, WU Rui, HE Xian-jing, WANG Shuang, LIN Yun-cheng, HAN Xu, WANG Yue-qiang, GUO Ting-ting, WU Guo-jun, YANG Ke-li\",\"doi\":\"10.1016/S1671-2927(11)60160-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Abstract</h3><p><em>Staphylococcus aureus, Escherichia coli</em> and <em>Bacillus cereus</em> are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of <em>S. aureus, E. coli</em> and <em>B. cereus,</em> and the detection limits were 10<sup>3</sup>, 10<sup>2</sup> and 10<sup>3</sup> CFU mL-<sup>1</sup>, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had <em>S. aureu</em> andii. <em>coli,</em> 9.39% had <em>E. coli</em> and <em>B. cereus,</em> and 9.71% had <em>S. aureus</em> and <em>B. cereus.</em> The rates of infections with <em>S. aureus, E. coli</em> and <em>B. cereuswere</em> 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.</p></div>\",\"PeriodicalId\":7475,\"journal\":{\"name\":\"Agricultural Sciences in China\",\"volume\":\"10 10\",\"pages\":\"Pages 1624-1629\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60160-0\",\"citationCount\":\"19\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Sciences in China\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1671292711601600\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601600","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis
Abstract
Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu andii. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereuswere 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.
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