Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

Agricultural Sciences in China Pub Date : 2011-10-01 DOI:10.1016/S1671-2927(11)60160-0

SUN Dong-bo, WU Rui, HE Xian-jing, WANG Shuang, LIN Yun-cheng, HAN Xu, WANG Yue-qiang, GUO Ting-ting, WU Guo-jun, YANG Ke-li

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引用次数: 19

Abstract

Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 10³, 10² and 10³ CFU mL-¹, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu andii. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereuswere 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.

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奶牛子宫内膜炎金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌多重PCR诊断方法的建立

摘要金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌是奶牛子宫内膜炎的主要病原。以金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌的保守基因为基础建立多重PCR (SEB-mPCR)，检出限分别为103、102和103 CFU mL-1。SEB-mPCR不能扩增其他牛常见疾病致病菌的基因组DNA。采用SEB-mPCR对309例子宫内膜炎奶牛阴道分泌物样本进行了检测。3种细菌检出率为23.95%，金黄色葡萄球菌检出率为17.15%。大肠杆菌和蜡样芽孢杆菌检出率为9.39%，金黄色葡萄球菌和蜡样芽孢杆菌检出率为9.71%。金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌的感染率分别为11.35%、16.18%和9.06%。因此，SEB-mPCR有可能作为奶牛子宫内膜炎的诊断工具。

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来源期刊

Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-

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审稿时长

3.2 months