A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers

Abstract

Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi-wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F₂ population consisting of 155 plants. The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD) markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between the adjacent markers.