Revue francophone des laboratoires : RFL最新文献

{"title":"Les gants ne remplacent pas l’hygiène des mains !","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(25)76422-9","DOIUrl":"10.1016/S1773-035X(25)76422-9","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 6"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Des vacuoles et des têtards","authors":"Zélie Mockelyn ,&nbsp;Marie-Adeline Migeon ,&nbsp;Pierre Lemaire ,&nbsp;Stéphanie Mathis ,&nbsp;Clémentine Chauvel ,&nbsp;Lou Soret ,&nbsp;Valérie Bardet","doi":"10.1016/S1773-035X(25)76445-X","DOIUrl":"10.1016/S1773-035X(25)76445-X","url":null,"abstract":"<div><h3>Résumé</h3><div>Un patient âgé de 85 ans, suivi pour un syndrome myélodysplasique (SMD), a présenté un tableau de pancytopénie associé à l’apparition concomitante d’une éruption cutanée. Les examens médullaires mettront en évidence une leucémie à cellules dendritiques plasmocytoïdes. Il s’agit d’une hémopathie rare associée à un pronostic sombre. Le diagnostic repose sur la cytométrie en flux et doit être évoqué devant un profil caractérisé par l’absence d’expression de marqueurs forts de lignées myéloïdes et lymphoïdes et par l’expression du CD4 et du CD56. Il est ensuite confirmé par l’expression de marqueurs spécifiques des cellules dendritiques plasmocytoïdes (CD123, CD303, CD304, TCL1, TCF4).</div></div><div><h3>Abstract</h3><div>An 85-year-old male patient, with a history of myelodysplastic syndrome (MDS), presented with pancytopenia accompanied by a rash. Bone marrow examination revealed blastic plasmacytoid dendritic cell neoplasm (BPDCN), a rare haematolo-gic malignancy associated with a poor prognosis. Immunophenotyping is mandatory to confirm the diagnosis. This condition should be considered in cases where the blast profile shows a lack of expression of specific markers of myeloid or lymphoid differentiation, combined with the expression of CD4 and CD56. The diagnosis is further confirmed by the presence of plasmacytoid dendritic cell lineage markers (CDI23, CD303, CD304,TCLI,TCF4).</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Pages 76-80"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Le Chikungunya dans l’Océan Indien","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(25)76435-7","DOIUrl":"10.1016/S1773-035X(25)76435-7","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 15"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CDKL5, une nouvelle maladie rare classifiée","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(25)76424-2","DOIUrl":"10.1016/S1773-035X(25)76424-2","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 7"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Des biomarqueurs musculaires pour un bilan précoce de gravité et d’évolution","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(25)76426-6","DOIUrl":"10.1016/S1773-035X(25)76426-6","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 9"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"La médecine du sport veut être reconnue comme une spécialité médicale","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(25)76431-X","DOIUrl":"10.1016/S1773-035X(25)76431-X","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 12"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L’allergologie biologique 2.0","authors":"Céline Beauvillain (Présidente d’AllergoBioNet)","doi":"10.1016/S1773-035X(25)76437-0","DOIUrl":"10.1016/S1773-035X(25)76437-0","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 25"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interopérabilité et données de santé : un rôle pivot des laboratoires d’analyses médicales","authors":"Sébastien Hantz","doi":"10.1016/S1773-035X(25)76419-9","DOIUrl":"10.1016/S1773-035X(25)76419-9","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Page 1"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pourquoi et comment rechercher et interpréter les sensibilisations aux allergènes émergents ?","authors":"Delphine Giusti ,&nbsp;Justine Cosme ,&nbsp;Stéphanie François ,&nbsp;Amir Guemari ,&nbsp;Stéphanie Rogeau","doi":"10.1016/S1773-035X(25)76440-0","DOIUrl":"10.1016/S1773-035X(25)76440-0","url":null,"abstract":"<div><h3>Résumé</h3><div>L’avènement de l’allergologie moléculaire il y a une trentaine d’années a considérablement modifié la prise en charge des patients en allergologie. À côté des familles moléculaires bien décrites : profilines, PR-10, LTP, protéines de stockage, de nouvelles familles d’allergènes moléculaires émergent. Ces dernières semblent pouvoir expliquer des profils cliniques complexes pour lesquels jusqu’alors les réactions restaient incomprises. Cette revue s’attachera à développer les protéines régulées par la gibbérelline, les chitinases, les défensines, les cyclophilines, les protéines de type thaumatine et les oléosines. Un focus sur les familles d’allergènes moléculaires décrites chez les moisissures sera également fait. Les allergènes moléculaires identifiés au sein de chacune de ces familles seront rapportés ainsi que ceux pour lesquels un dosage commercial est disponible.</div></div><div><h3>Abstract</h3><div>The emergence of molecular allergology some thirty years ago has significantly changed patient care in allergology. In addition to the well-described molecular families: profilins, PR-10, LTP, storage proteins, new families of molecular allergens are emerging. These appear to be able to explain complex clinical profiles for which reactions previously remained poorly understood. This review will focus on giberellin regulated-proteins, chitinases, defensins, cyclophilins, thaumatin-like proteins and oleosins. A focus on the families of molecular allergens described in molds will also be made. The molecular allergens identified within each of these families will be reported as well as those for which a commercial assay is available.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Pages 48-58"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Détermination d’un seuil de positivité en ELISA des anticorps anti-Myelin associated glycoprotein","authors":"Sara Hooper ,&nbsp;Adrien Thiriet ,&nbsp;Benoît Nespola ,&nbsp;Philippe Moine ,&nbsp;Jérôme Joël Devaux ,&nbsp;Thierry Vincent ,&nbsp;Pascale Chrétien ,&nbsp;Alexandre Jentzer","doi":"10.1016/S1773-035X(25)76436-9","DOIUrl":"10.1016/S1773-035X(25)76436-9","url":null,"abstract":"<div><h3>Résumé</h3><div>La neuropathie à anticorps <em>anti-Myelin Associated Glycoprotein</em> (MAG) est une neuropathie périphérique dysimmunitaire rare associée à une gammapathie monoclonale. Le diagnostic de cette neuropathie repose sur des arguments clinico-électriques et sur la détection d’anticorps anti-MAG (quantifiés en ELISA). Le seuil de positivité du fournisseur est de 1 000 Bühlmann <em>titer unit</em> (BTU). L’objectif de notre étude était de proposer un seuil de positivité adapté à la pratique médicale courante. Sur la période de janvier 2022 à décembre 2024, une cohorte de 303 sujets adultes des CHU de Montpellier, Strasbourg et AP-HP-Kremlin-Bicêtre a été constituée dont 103 patients atteints de neuropathie à anticorps anti-MAG. Le projet a été validé par le comité scientifique et éthique de Montpellier (CSE n° A029/2024-08-124/002). Les analyses ont été réalisées avec le kit ELISA Bühlmann®. Le seuil fournisseur de 1 000 BTU montre une sensibilité de 99 % (95 % IC : 95 % à 100 %) et une spécificité de 64 % (95 % IC : 57 % à 70 %) avec un indice de Youden à 0,6253. Un seuil de 3 000 BTU a une sensibilité à 95 % (95 % IC : 89 % à 98 %) et une spécificité à 84 % (95 % IC : 78 % à 88 %) avec un indice de Youden à 0,7915. Le seuil de 1 000 BTU montrait un défaut de spécificité corrigé en élevant le seuil à 3 000 BTU tout en conservant une excellente sensibilité. Notre étude a permis de proposer un seuil optimal de positivité des anticorps anti-MAG adapté à la pratique clinique.</div></div><div><h3>Abstract</h3><div>Anti-MAG antibody neuropathy is a rare peripheral dysimmune neuropathy associated with monoclonal gammopathy. The diagnosis of this neuropathy relies on clinical-electrical evidence and the detection of anti-MAG antibodies (quantified by ELISA). The supplier’s positivity threshold is 1 000 Bühlmann titer units (BTU). The aim of our study was to refine the supplier’s threshold and propose a threshold more suitable for routine clinical practice. From January 2022 to December 2024, a cohort of 303 adult subjects from the University hospitals of Montpellier, Strasbourg, and APHP-Kremlin-Bicêtre was established (including 103 patients with anti-MAG neuropathy). The project was approved by the Scientific and ethical committee of Montpellier (CSE N°. A029/2024-08-124/002).The analyses were conducted using the Bühlmann’s ELISA kit®. The supplier’s threshold of 1 000 BTU shows a sensitivity of 99 % (95 % CI : 95 % to 100 %) and a specificity of 64 % (95 % CI : 57 % to 70 %) with a Youden index of 0,6253. A threshold of 3 000 BTU has a sensitivity of 95 % (95 % CI : 89 % to 98 %) and a specificity of 84 % (95 % CI : 78 % to 88 %) with a Youden index of 0,7915. The 1 000 BTU threshold showed a specificity deficit, which was corrected acceptably by raising the threshold to 3 000 BTU. Our study allowed for the proposal of a positivity threshold for anti-MAG antibodies better suited to routine clinical practice.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2025 574","pages":"Pages 16-23"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}