ISME communications最新文献_第3页

Correction to: Rhizospheric miRNAs affect the plant microbiota. 根际mirna影响植物微生物群。

IF 5.1

ISME communications Pub Date : 2024-12-04 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae144

引用次数: 0

Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins. 通过BONCAT追踪微生物群落中的活性成员，并点击基于化学的新合成蛋白质富集。

IF 5.1

ISME communications Pub Date : 2024-12-04 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae153

Patrick Hellwig, Daniel Kautzner, Robert Heyer, Anna Dittrich, Daniel Wibberg, Tobias Busche, Anika Winkler, Udo Reichl, Dirk Benndorf

{"title":"Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.","authors":"Patrick Hellwig, Daniel Kautzner, Robert Heyer, Anna Dittrich, Daniel Wibberg, Tobias Busche, Anika Winkler, Udo Reichl, Dirk Benndorf","doi":"10.1093/ismeco/ycae153","DOIUrl":"10.1093/ismeco/ycae153","url":null,"abstract":"A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and biotechnology. Metaproteomics, defined as the analysis of all proteins present within a microbial community, provides insights into these complex systems. Microbial adaptation and activity depend to an important extent on newly synthesized proteins (nP), however, the distinction between nP and bulk proteins is challenging. The application of BONCAT with click chemistry has demonstrated efficacy in the enrichment of nP in pure cultures for proteomics. However, the transfer of this technique to microbial communities and metaproteomics has proven challenging and thus it has not not been used on microbial communities before. To address this, a new workflow with efficient and specific nP enrichment was developed using a laboratory-scale mixture of labelled Escherichia coli and unlabeled yeast. This workflow was then successfully applied to an anaerobic microbial community with initially low bioorthogonal non-canonical amino acid tagging efficiency. A substrate shift from glucose to ethanol selectively enriched nP with minimal background. The identification of bifunctional alcohol dehydrogenase and a syntrophic interaction between an ethanol-utilizing bacterium and two methanogens (hydrogenotrophic and acetoclastic) demonstrates the potential of metaproteomics targeting nP to trace microbial activity in complex microbial communities.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae153"},"PeriodicalIF":5.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomics reveals the genetic diversity between sublineages of UCYN-A and their algal host plastids. 宏基因组学揭示了UCYN-A亚系及其藻类宿主质体之间的遗传多样性。

IF 5.1

ISME communications Pub Date : 2024-12-04 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae150

Ella Joy H Kantor, Brent M Robicheau, Jennifer Tolman, John M Archibald, Julie LaRoche

{"title":"Metagenomics reveals the genetic diversity between sublineages of UCYN-A and their algal host plastids.","authors":"Ella Joy H Kantor, Brent M Robicheau, Jennifer Tolman, John M Archibald, Julie LaRoche","doi":"10.1093/ismeco/ycae150","DOIUrl":"10.1093/ismeco/ycae150","url":null,"abstract":"UCYN-A (or Cand. Atelocyanobacterium thalassa) has been recognized as a globally distributed, early stage, nitrogen-fixing organelle (the \"nitroplast\") of cyanobacterial origin present in the haptophyte alga Braarudosphaera bigelowii. Although the nitroplast was recognized as UCYN-A2, not all sublineages of UCYN-A have been confirmed as nitroplasts, and full genomes are still lacking for several known sublineages. We investigated the differences between UCYN-A sublineages by sequencing and assembly of metagenomic sequences acquired from cultured biomass from NW Atlantic seawater, which yielded near-complete Metagenome Assembled Genomes (MAGs) corresponding to UCYN-A1, -A4, and the plastid of the UCYN-A4-associated B. bigelowii. Weekly time-series data paired with the recurrence of specific microbes in cultures used for metagenomics gave further insight into the microbial community associated with the algal/UCYN-A complex. The UCYN-A1 MAG was found to have 99% average nucleotide identity (ANI) to the Pacific-derived reference genome despite its Atlantic Ocean origin. Comparison of the UCYN-A4 MAG (the initial genome sequenced from this sublineage) to other genomes showed that UCYN-A4 is sufficiently genetically distinct from both UCYN-A1 and UCYN-A2 (ANI of ~83% and ~85%, respectively) to be considered its own sublineage, but more similar to UCYN-A2 than -A1, supporting its possible classification as a nitroplast. The B. bigelowii plastid sequence was compared with published plastid sequences (sharing 78% ANI with Chrysochromulina parva) adding to our understanding of genomic variation across Haptophyta organelles and emphasizing the need for further full genomic sequencing of B. bigelowii genotypes and their organelles.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae150"},"PeriodicalIF":5.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal dynamics in a red alga dominated geothermal feature in Yellowstone National Park. 黄石国家公园红藻主导地热特征的时间动态。

IF 5.1

ISME communications Pub Date : 2024-12-03 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae151

Timothy G Stephens, Julia Van Etten, Timothy McDermott, William Christian, Martha Chaverra, James Gurney, Yongsung Lee, Hocheol Kim, Chung Hyun Cho, Erik Chovancek, Philipp Westhoff, Antonia Otte, Trent R Northen, Benjamin P Bowen, Katherine B Louie, Kerrie Barry, Igor V Grigoriev, Thomas Mock, Shao-Lun Liu, Shin-Ya Miyagishima, Masafumi Yoshinaga, Andreas P M Weber, Hwan Su Yoon, Debashish Bhattacharya

{"title":"Temporal dynamics in a red alga dominated geothermal feature in Yellowstone National Park.","authors":"Timothy G Stephens, Julia Van Etten, Timothy McDermott, William Christian, Martha Chaverra, James Gurney, Yongsung Lee, Hocheol Kim, Chung Hyun Cho, Erik Chovancek, Philipp Westhoff, Antonia Otte, Trent R Northen, Benjamin P Bowen, Katherine B Louie, Kerrie Barry, Igor V Grigoriev, Thomas Mock, Shao-Lun Liu, Shin-Ya Miyagishima, Masafumi Yoshinaga, Andreas P M Weber, Hwan Su Yoon, Debashish Bhattacharya","doi":"10.1093/ismeco/ycae151","DOIUrl":"10.1093/ismeco/ycae151","url":null,"abstract":"Alga-dominated geothermal spring communities in Yellowstone National Park (YNP), USA, have been the focus of many studies, however, relatively little is known about the composition and community interactions which underpin these ecosystems. Our goal was to determine, in three neighboring yet distinct environments in Lemonade Creek, YNP, how cells cope with abiotic stressors over the diurnal cycle. All three environments are colonized by two photosynthetic lineages, Cyanidioschyzon and Galdieria, both of which are extremophilic Cyanidiophyceae red algae. Cyanidioschyzon, a highly specialized obligate photoautotroph, dominated cell counts at all three Lemonade Creek environments. The cell cycle of Cyanidioschyzon in YNP matched that observed in synchronized cultures, suggesting that light availability plays a strong role in constraining growth of this alga in its natural habitat. Surprisingly, the mixotrophic and physiologically more flexible Galdieria, was a minor component of these algal populations. Arsenic detoxification at Lemonade Creek occurred via complementary gene expression by different eukaryotic and prokaryotic lineages, consistent with this function being shared by the microbial community, rather than individual lineages completing the entire pathway. These results demonstrate the highly structured nature of these extreme habitats, particularly regarding arsenic detoxification.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae151"},"PeriodicalIF":5.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aromatic amino acid metabolism and active transport regulation are implicated in microbial persistence in fractured shale reservoirs. 芳香族氨基酸代谢和主动转运调控与裂缝性页岩储层微生物持续性有关。

IF 5.1

ISME communications Pub Date : 2024-11-26 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae149

Chika Jude Ugwuodo, Fabrizio Colosimo, Jishnu Adhikari, Samuel O Purvine, Elizabeth K Eder, David W Hoyt, Stephanie A Wright, Mary S Lipton, Paula J Mouser

{"title":"Aromatic amino acid metabolism and active transport regulation are implicated in microbial persistence in fractured shale reservoirs.","authors":"Chika Jude Ugwuodo, Fabrizio Colosimo, Jishnu Adhikari, Samuel O Purvine, Elizabeth K Eder, David W Hoyt, Stephanie A Wright, Mary S Lipton, Paula J Mouser","doi":"10.1093/ismeco/ycae149","DOIUrl":"10.1093/ismeco/ycae149","url":null,"abstract":"Hydraulic fracturing has unlocked vast amounts of hydrocarbons trapped within unconventional shale formations. This large-scale engineering approach inadvertently introduces microorganisms into the hydrocarbon reservoir, allowing them to inhabit a new physical space and thrive in the unique biogeochemical resources present in the environment. Advancing our fundamental understanding of microbial growth and physiology in this extreme subsurface environment is critical to improving biofouling control efficacy and maximizing opportunities for beneficial natural resource exploitation. Here, we used metaproteomics and exometabolomics to investigate the biochemical mechanisms underpinning the adaptation of model bacterium Halanaerobium congolense WG10 and mixed microbial consortia enriched from shale-produced fluids to hypersalinity and very low reservoir flow rates (metabolic stress). We also queried the metabolic foundation for biofilm formation in this system, a major impediment to subsurface energy exploration. For the first time, we report that H. congolense WG10 accumulates tyrosine for osmoprotection, an indication of the flexible robustness of stress tolerance that enables its long-term persistence in fractured shale environments. We also identified aromatic amino acid synthesis and cell wall maintenance as critical to biofilm formation. Finally, regulation of transmembrane transport is key to metabolic stress adaptation in shale bacteria under very low well flow rates. These results provide unique insights that enable better management of hydraulically fractured shale systems, for more efficient and sustainable energy extraction.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae149"},"PeriodicalIF":5.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal of Patescibacterium danicum gen. nov., sp. nov. in the ubiquitous bacterial phylum Patescibacteriota phyl. nov.

IF 5.1

ISME communications Pub Date : 2024-11-19 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae147

Zuzanna Dutkiewicz, Caitlin M Singleton, Mantas Sereika, Juan C Villada, Aaron J Mussig, Maria Chuvochina, Mads Albertsen, Frederik Schulz, Tanja Woyke, Per H Nielsen, Philip Hugenholtz, Christian Rinke

{"title":"Proposal of Patescibacterium danicum gen. nov., sp. nov. in the ubiquitous bacterial phylum Patescibacteriota phyl. nov.","authors":"Zuzanna Dutkiewicz, Caitlin M Singleton, Mantas Sereika, Juan C Villada, Aaron J Mussig, Maria Chuvochina, Mads Albertsen, Frederik Schulz, Tanja Woyke, Per H Nielsen, Philip Hugenholtz, Christian Rinke","doi":"10.1093/ismeco/ycae147","DOIUrl":"10.1093/ismeco/ycae147","url":null,"abstract":"Candidatus Patescibacteria is a diverse bacterial phylum that is notable for members with ultrasmall cell size, reduced genomes, limited metabolic capabilities, and dependence on other prokaryotic hosts. Despite the prevalence of the name Ca. Patescibacteria in the scientific literature, it is not officially recognized under the International Code of Nomenclature of Prokaryotes and lacks a nomenclatural type. Here, we rectify this situation by describing two closely related circular metagenome-assembled genomes and by proposing one of them (ABY1TS) to serve as the nomenclatural type for the species Patescibacterium danicum TS gen. nov., sp. nov. according to the rules of the SeqCode. Rank-normalized phylogenomic inference confirmed the stable placement of P. danicum TS in the Ca. Patescibacteria class ABY1. Based on these results, we propose Patescibacterium gen. nov. to serve as the type genus for associated higher taxa, including the phylum Patescibacteriota phyl. nov. We complement our proposal with a genomic characterization, metabolic reconstruction, and biogeographical analysis of Patescibacterium. Our results confirm small genome sizes (<1 Mbp), low GC content (>36%), and the occurrence of long gene coding insertions in the 23S rRNA sequences, along with reduced metabolic potential, inferred symbiotic lifestyle, and a global distribution. In summary, our proposal will provide nomenclatural stability to the fourth-largest phylum in the bacterial domain.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae147"},"PeriodicalIF":5.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11809585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does periphyton turn less palatable under grazing pressure? 在放牧压力下，周围植物会变得不那么美味吗？

IF 5.1

ISME communications Pub Date : 2024-11-19 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae146

Feng Zhu, Xiang Tan, Xingzhong Wang, Quanfa Zhang

{"title":"Does periphyton turn less palatable under grazing pressure?","authors":"Feng Zhu, Xiang Tan, Xingzhong Wang, Quanfa Zhang","doi":"10.1093/ismeco/ycae146","DOIUrl":"https://doi.org/10.1093/ismeco/ycae146","url":null,"abstract":"Periphyton acts as an important primary producer in stream food webs with bottom-up grazing pressure and is also subject to effects of top-down grazing pressure. However, the underlying mechanisms of these interactions remain unclear. In this study we conducted a mesocosm experiment to explore the periphyton response to grazing pressure by the freshwater snail Bellamya aeruginosa in relation to food quality indicated by polyunsaturated fatty acid (PUFA) biomarkers, including eicosapentaenoic acid (20:5n3) and the 22C fatty acid docosahexaenoic acid (22:6n3), which are essential for cell growth and reproduction and cannot be synthesized by most consumers of periphyton. Results indicated that periphyton grazing pressure led to a decrease in Bacillariophyta, which contain high-quality PUFAs such as eicsapentaenoic acid and docosahexaenoic acid, and an increase in Cyanophyta and Chlorophyta, which are rich in 18C PUFAs such as linoleic acid (18:2n6) and alpha-linolenic acid (18:3n3). We observed upregulation of genes that participate in lipid metabolism promoting unsaturated fatty acid biosynthesis, alpha-linolenic acid metabolism, and glycerophospholipid metabolism, which are related to the carbohydrate and energy metabolism maintaining the energy stability of periphyton. These results demonstrate that the food quality of periphyton decreased under grazing pressure and also elucidate the compositional, chemical, and molecular perspectives of the interactive bottom-up and top-down effects on structuring stream food webs.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae146"},"PeriodicalIF":5.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative past, present, and future spatial distributions of deep-sea coral and sponge microbiomes revealed by predictive models. 预测模型揭示的深海珊瑚和海绵微生物群落的假定过去、现在和未来的空间分布。

IF 5.1

ISME communications Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae142

Kathrin Busch, Francisco Javier Murillo, Camille Lirette, Zeliang Wang, Ellen Kenchington

{"title":"Putative past, present, and future spatial distributions of deep-sea coral and sponge microbiomes revealed by predictive models.","authors":"Kathrin Busch, Francisco Javier Murillo, Camille Lirette, Zeliang Wang, Ellen Kenchington","doi":"10.1093/ismeco/ycae142","DOIUrl":"10.1093/ismeco/ycae142","url":null,"abstract":"Knowledge of spatial distribution patterns of biodiversity is key to evaluate and ensure ocean integrity and resilience. Especially for the deep ocean, where in situ monitoring requires sophisticated instruments and considerable financial investments, modeling approaches are crucial to move from scattered data points to predictive continuous maps. Those modeling approaches are commonly run on the macrobial level, but spatio-temporal predictions of host-associated microbiomes are not being targeted. This is especially problematic as previous research has highlighted that host-associated microbes may display distribution patterns that are not perfectly correlated not only with host biogeographies, but also with other factors, such as prevailing environmental conditions. We here establish a new simulation approach and present predicted spatio-temporal distribution patterns of deep-sea sponge and coral microbiomes, making use of a combination of environmental data, host data, and microbiome data. This approach allows predictions of microbiome spatio-temporal distribution patterns on scales that are currently not covered by classical sampling approaches at sea. In summary, our presented predictions allow (i) identification of microbial biodiversity hotspots in the past, present, and future, (ii) trait-based predictions to link microbial with macrobial biodiversity, and (iii) identification of shifts in microbial community composition (key taxa) across environmental gradients and shifting environmental conditions.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae142"},"PeriodicalIF":5.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11694675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral diversity and host associations in microbial electrolysis cells. 微生物电解细胞中的病毒多样性和宿主关联。

IF 5.1

ISME communications Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae143

Marie Abadikhah, Frank Persson, Anne Farewell, Britt-Marie Wilén, Oskar Modin

{"title":"Viral diversity and host associations in microbial electrolysis cells.","authors":"Marie Abadikhah, Frank Persson, Anne Farewell, Britt-Marie Wilén, Oskar Modin","doi":"10.1093/ismeco/ycae143","DOIUrl":"10.1093/ismeco/ycae143","url":null,"abstract":"In microbial electrolysis cells (MECs), microbial communities catalyze conversions between dissolved organic compounds, electrical energy, and energy carriers such as hydrogen and methane. Bacteria and archaea, which catalyze reactions on the anode and cathode of MECs, interact with phages; however, phage communities have previously not been examined in MECs. In this study, we used metagenomic sequencing to study prokaryotes and phages in nine MECs. A total of 852 prokaryotic draft genomes representing 278 species, and 1476 phage contigs representing 873 phage species were assembled. Among high quality prokaryotic genomes (>95% completion), 55% carried a prophage, and the three Desulfobacterota spp. that dominated the anode communities all carried prophages. Geobacter anodireducens, one of the bacteria dominating the anode communities, carried a CRISPR spacer showing evidence of a previous infection by a Peduoviridae phage present in the liquid of some MECs. Methanobacteriaceae spp. and an Acetobacterium sp., which dominated the cathodes, had several associations with Straboviridae spp. The results of this study show that phage communities in MECs are diverse and interact with functional microorganisms on both the anode and cathode.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae143"},"PeriodicalIF":5.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archives of cyanobacterial traits: insights from resurrected Nodularia spumigena from Baltic Sea sediments reveal a shift in temperature optima. 蓝藻特征档案：从波罗的海沉积物中复活的spumigena的见解揭示了温度最佳状态的变化。

IF 5.1

ISME communications Pub Date : 2024-11-13 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae140

Cynthia Medwed, Ulf Karsten, Juliane Romahn, Jérôme Kaiser, Olaf Dellwig, Helge Arz, Anke Kremp

{"title":"Archives of cyanobacterial traits: insights from resurrected Nodularia spumigena from Baltic Sea sediments reveal a shift in temperature optima.","authors":"Cynthia Medwed, Ulf Karsten, Juliane Romahn, Jérôme Kaiser, Olaf Dellwig, Helge Arz, Anke Kremp","doi":"10.1093/ismeco/ycae140","DOIUrl":"https://doi.org/10.1093/ismeco/ycae140","url":null,"abstract":"Cyanobacterial blooms in the Baltic Sea proliferated in recent decades due to rising sea surface temperatures, resulting in significant ecological impacts. To elucidate their current success, we examined ecophysiological, biochemical, and morphological traits of recent and ~33-year-old strains of Nodularia spumigena using a resurrection approach. The ability of many cyanobacteria to form dormant stages that can persist in anoxic sediments for decades provides a unique opportunity to study adaptive traits to past environmental conditions. A short sediment core from the Eastern Gotland Basin was processed to isolate strains of N. spumigena buried in 1987 ± 2 and 2020 ± 0.5 Common Era. Sequencing was used for species identification, followed by characterization of cell morphometry, carbon, nitrogen, and chlorophyll a content. Photosynthetic performance was evaluated by using pulse-amplitude modulated fluorimetry and oxygen optodes to assess light and temperature requirements. Our results revealed trait changes in N. spumigena over the past 3 decades: Temperature optimum for photosynthesis shifted from 15.3-21.1°C, which is consistent with the past and present local SST. Recent strains exhibited increased carbon, nitrogen, and chlorophyll a content despite decreased cell volume. The demonstrated adaptability of N. spumigena to increasing temperature suggests that this species will thrive in a warmer climate in the future. These insights will aid modeling efforts aimed at understanding and managing consequences of future cyanobacterial blooms in the Baltic Sea ecosystem.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae140"},"PeriodicalIF":5.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11605557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0