ISME communications最新文献_第2页

Microcystin shapes the Microcystis phycosphere through community filtering and by influencing cross-feeding interactions. 微囊藻毒素通过群落过滤和影响交叉摄食相互作用来塑造微囊藻的藻圈。

IF 5.1

ISME communications Pub Date : 2024-12-24 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae170

Rebecca Große, Markus Heuser, Jonna E Teikari, Dinesh K Ramakrishnan, Ahmed Abdelfattah, Elke Dittmann

{"title":"Microcystin shapes the Microcystis phycosphere through community filtering and by influencing cross-feeding interactions.","authors":"Rebecca Große, Markus Heuser, Jonna E Teikari, Dinesh K Ramakrishnan, Ahmed Abdelfattah, Elke Dittmann","doi":"10.1093/ismeco/ycae170","DOIUrl":"10.1093/ismeco/ycae170","url":null,"abstract":"The cyanobacterium Microcystis causes harmful algal blooms that pose a major threat to human health and ecosystem services, particularly due to the prevalence of the potent hepatotoxin microcystin (MC). With their pronounced EPS layer, Microcystis colonies also serve as a hub for heterotrophic phycosphere bacteria. Here, we tested the hypothesis that the genotypic plasticity in its ability to produce MC influences the composition and assembly of the Microcystis phycosphere microbiome. In an analysis of individual colonies of a natural Microcystis bloom, we observed a significantly reduced richness of the community in the presence of MC biosynthesis genes. A subsequent synthetic community experiment with 21 heterotrophic bacterial strains in co-cultivation with either the wild-type strain Microcystis aeruginosa PCC 7806 or the MC-free mutant ΔmcyB revealed not only a tug-of-war between phototrophic and heterotrophic bacteria, but also a reciprocal dominance of two isolates of the genus Sphingomonas and Flavobacterium. In contrast, an Agrobacterium isolate thrived equally well in both consortia. In substrate utilization tests, Sphingomonas showed the strongest dependence on Microcystis exudates with a clear preference for the wild-type strain. Genome sequencing revealed a high potential for complementary cross-feeding, particularly for the Agrobacterium and Sphingomonas isolates but no potential for MC degradation. We postulate that strain-specific functional traits, such as the ability to perform glycolate oxidation, play a crucial role in the cross-feeding interactions, and that MC is one of the determining factors in the Microcystis phycosphere due to its interference with inorganic carbon metabolism.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae170"},"PeriodicalIF":5.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial and viral assemblages in ulcerative colitis patients following fecal microbiota and fecal filtrate transfer. 溃疡性结肠炎患者粪便微生物群和粪便滤液转移后的细菌和病毒组合。

IF 5.1

ISME communications Pub Date : 2024-12-23 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae167

Howard Junca, Arndt Steube, Simon Mrowietz, Johannes Stallhofer, Marius Vital, Luiz Gustavo Dos Anjos Borges, Dietmar H Pieper, Andreas Stallmach

{"title":"Bacterial and viral assemblages in ulcerative colitis patients following fecal microbiota and fecal filtrate transfer.","authors":"Howard Junca, Arndt Steube, Simon Mrowietz, Johannes Stallhofer, Marius Vital, Luiz Gustavo Dos Anjos Borges, Dietmar H Pieper, Andreas Stallmach","doi":"10.1093/ismeco/ycae167","DOIUrl":"10.1093/ismeco/ycae167","url":null,"abstract":"Fecal microbiota filtrate transfer is discussed as a safe alternative to fecal microbiota transfer (FMT) to treat ulcerative colitis. We investigated modulation of viral and bacterial composition during fecal microbiota filtrate transfer followed by FMT in six patients with active ulcerative colitis (where clinical activity improved in three patients after filtrate transfer) and combined 16S ribosomal RNA gene amplicon sequencing with a virome analysis pipeline including fast viral particle enrichment and metagenome mapping to detect frequencies of 45,033 reference bacteriophage genomes. We showed that after antibiotic treatment and during filtrate transfer, the bacterial community typically adopted a stable composition distinct to that before antibiotic treatment, with no change toward a donor community. FMT in contrast typically changed the bacterial community to a community with similarity to donor(s). There were no indications of an establishment of predominant donor viruses during filtrate transfer but a remodeling of the virome. In contrast, the establishment of donor viruses during FMT correlated with the predicted hosts established during such transfer. Our approach warrants further investigation in a randomized trial to evaluate larger therapeutic interventions in a comparable and efficient manner.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae167"},"PeriodicalIF":5.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyletic patterns of bacterial growth temperature in Pseudomonas and Paenibacillus reveal gradual and sporadic evolution towards cold adaptation. 假单胞菌和芽孢杆菌的细菌生长温度的进化模式揭示了逐渐和零星的向冷适应的进化。

IF 5.1

ISME communications Pub Date : 2024-12-23 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae163

Kihyun Lee, Seong-Hyeon Kim, Seongjoon Moon, Sangha Kim, Changhan Lee

引用次数: 0

The microbiome of a perennial cereal differs from annual winter wheat only in the root endosphere.

IF 5.1

ISME communications Pub Date : 2024-12-23 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae165

Kristina Michl, Makoto Kanasugi, Alena Förster, Regina Wuggenig, Sulemana Issifu, Katarzyna Hrynkiewicz, Christoph Emmerling, Christophe David, Benjamin Dumont, Linda-Maria Dimitrova Mårtensson, Frank Rasche, Gabriele Berg, Tomislav Cernava

{"title":"The microbiome of a perennial cereal differs from annual winter wheat only in the root endosphere.","authors":"Kristina Michl, Makoto Kanasugi, Alena Förster, Regina Wuggenig, Sulemana Issifu, Katarzyna Hrynkiewicz, Christoph Emmerling, Christophe David, Benjamin Dumont, Linda-Maria Dimitrova Mårtensson, Frank Rasche, Gabriele Berg, Tomislav Cernava","doi":"10.1093/ismeco/ycae165","DOIUrl":"10.1093/ismeco/ycae165","url":null,"abstract":"The intensification of agriculture has led to environmental degradation, including the loss of biodiversity. This has prompted interest in perennial grain cropping systems to address and mitigate some of these negative impacts. In order to determine if perennial grain cultivation promotes a higher microbial diversity, we assessed the endophytic microbiota of a perennial grain crop (intermediate wheatgrass, Thinopyrum intermedium L.) in comparison to its annual counterpart, wheat (Triticum aestivum L.). The study covered three sampling sites in a pan-European gradient (Sweden, Belgium, and France), two plant genotypes, three plant compartments (roots, stems, and leaves), and two sampling time points. We observed that the host genotype effect was mainly evident in the belowground compartment, and only to a lesser extent in the aboveground tissues, with a similar pattern at all three sampling sites. Moreover, intermediate wheatgrass roots harbored a different bacterial community composition and higher diversity and richness compared to their annual counterparts. The root bacterial diversity was influenced by not only several soil chemical parameters, such as the carbon:nitrogen ratio, but also soil microbial parameters, such as soil respiration and dehydrogenase activity. Consistent findings across time and space suggest stable mechanisms in microbiota assembly associated with perennial grain cropping, underscoring their potential role in supporting biodiversity within sustainable agricultural systems.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae165"},"PeriodicalIF":5.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Friends and foes: symbiotic and algicidal bacterial influence on Karenia brevis blooms. 朋友和敌人：共生和杀藻细菌对短凯伦氏菌华的影响。

IF 5.1

ISME communications Pub Date : 2024-12-18 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae164

Cong Fei, Anne Booker, Sarah Klass, Nayani K Vidyarathna, So Hyun Ahn, Amin R Mohamed, Muhammad Arshad, Patricia M Glibert, Cynthia A Heil, Joaquín Martínez Martínez, Shady A Amin

{"title":"Friends and foes: symbiotic and algicidal bacterial influence on Karenia brevis blooms.","authors":"Cong Fei, Anne Booker, Sarah Klass, Nayani K Vidyarathna, So Hyun Ahn, Amin R Mohamed, Muhammad Arshad, Patricia M Glibert, Cynthia A Heil, Joaquín Martínez Martínez, Shady A Amin","doi":"10.1093/ismeco/ycae164","DOIUrl":"10.1093/ismeco/ycae164","url":null,"abstract":"Harmful Algal Blooms (HABs) of the toxigenic dinoflagellate Karenia brevis (KB) are pivotal in structuring the ecosystem of the Gulf of Mexico (GoM), decimating coastal ecology, local economies, and human health. Bacterial communities associated with toxigenic phytoplankton species play an important role in influencing toxin production in the laboratory, supplying essential factors to phytoplankton and even killing blooming species. However, our knowledge of the prevalence of these mechanisms during HAB events is limited, especially for KB blooms. Here, we introduced native microbial communities from the GoM, collected during two phases of a Karenia bloom, into KB laboratory cultures. Using bacterial isolation, physiological experiments, and shotgun metagenomic sequencing, we identified both putative enhancers and mitigators of KB blooms. Metagenome-assembled genomes from the Roseobacter clade showed strong correlations with KB populations during HABs, akin to symbionts. A bacterial isolate from this group of metagenome-assembled genomes, Mameliella alba, alleviated vitamin limitations of KB by providing it with vitamins B1, B7 and B12. Conversely, bacterial isolates belonging to Bacteroidetes and Gammaproteobacteria, Croceibacter atlanticus, and Pseudoalteromonas spongiae, respectively, exhibited strong algicidal properties against KB. We identified a serine protease homolog in P. spongiae that putatively drives the algicidal activity in this isolate. While the algicidal mechanism in C. atlanticus is unknown, we demonstrated the efficiency of C. atlanticus to mitigate KB growth in blooms from the GoM. Our results highlight the importance of specific bacteria in influencing the dynamics of HABs and suggest strategies for future HAB management.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae164"},"PeriodicalIF":5.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial synergies amplify nitrogenase activity in diverse systems. 细菌的协同作用增强了不同系统中氮酶的活性。

IF 5.1

ISME communications Pub Date : 2024-12-12 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae158

Andrew W Sher, Robert J Tournay, Emma Gomez-Rivas, Sharon L Doty

{"title":"Bacterial synergies amplify nitrogenase activity in diverse systems.","authors":"Andrew W Sher, Robert J Tournay, Emma Gomez-Rivas, Sharon L Doty","doi":"10.1093/ismeco/ycae158","DOIUrl":"10.1093/ismeco/ycae158","url":null,"abstract":"Endophytes are microbes living within plant tissue, with some having the capacity to fix atmospheric nitrogen in both a free-living state and within their plant host. They are part of a diverse microbial community whose interactions sometimes result in a more productive symbiosis with the host plant. Here, we report the co-isolation of diazotrophic endophytes with synergistic partners sourced from two separate nutrient-limited sites. In the presence of these synergistic strains, the nitrogen-fixing activity of the diazotroph is amplified. One such partnership was co-isolated from extracts of plants from a nutrient-limited Hawaiian lava field and another from the roots of Populus trees on a nutrient-limited gravel bar in the Pacific Northwest. The synergistic strains were capable of increasing the nitrogenase activity of different diazotrophic species from other environments, perhaps indicating that these endophytic microbial interactions are common to environments where nutrients are particularly limited. Multiple overlapping mechanisms seem to be involved in this interaction. Though synergistic strains are likely capable of protecting nitrogenase from oxygen, another mechanism seems evident in both environments. The synergies do not depend exclusively on physical contact, indicating a secreted compound may be involved. This work offers insights into beneficial microbial interactions, providing potential avenues for optimizing inocula for use in agriculture.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae158"},"PeriodicalIF":5.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meta-omics reveals role of photosynthesis in microbially induced carbonate precipitation at a CO₂-rich geyser. 元组学揭示了光合作用在富含二氧化碳的间歇泉微生物诱导碳酸盐沉淀中的作用。

IF 5.1

ISME communications Pub Date : 2024-12-11 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae139

Marlene J Violette, Ethan Hyland, Landon Burgener, Adit Ghosh, Brina M Montoya, Manuel Kleiner

{"title":"Meta-omics reveals role of photosynthesis in microbially induced carbonate precipitation at a CO2-rich geyser.","authors":"Marlene J Violette, Ethan Hyland, Landon Burgener, Adit Ghosh, Brina M Montoya, Manuel Kleiner","doi":"10.1093/ismeco/ycae139","DOIUrl":"10.1093/ismeco/ycae139","url":null,"abstract":"Microbially induced carbonate precipitation (MICP) is a natural process with potential biotechnological applications to address both carbon sequestration and sustainable construction needs. However, our understanding of the microbial processes involved in MICP is limited to a few well-researched pathways such as ureolytic hydrolysis. To expand our knowledge of MICP, we conducted an omics-based study on sedimentary communities from travertine around the CO2-driven Crystal Geyser near Green River, Utah. Using metagenomics and metaproteomics, we identified the community members and potential metabolic pathways involved in MICP. We found variations in microbial community composition between the two sites we sampled, but Rhodobacterales were consistently the most abundant order, including both chemoheterotrophs and anoxygenic phototrophs. We also identified several highly abundant genera of Cyanobacteriales. The dominance of these community members across both sites and the abundant presence of photosynthesis-related proteins suggest that photosynthesis could play a role in MICP at Crystal Geyser. We also found abundant bacterial proteins involved in phosphorous starvation response at both sites suggesting that P-limitation shapes both composition and function of the microbial community driving MICP.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae139"},"PeriodicalIF":5.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key bacteria decomposing animal and plant detritus in deep sea revealed via long-term in situ incubation in different oceanic areas. 通过在不同海域的长期原位孵育，揭示了深海中分解动物和植物碎屑的关键细菌。

IF 5.1

ISME communications Pub Date : 2024-12-10 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae133

Jianyang Li, Chunming Dong, Shizheng Xiang, Huiyang Wei, Qiliang Lai, Guangshan Wei, Linfeng Gong, Zhaobin Huang, Donghui Zhou, Guangyi Wang, Zongze Shao

{"title":"Key bacteria decomposing animal and plant detritus in deep sea revealed via long-term in situ incubation in different oceanic areas.","authors":"Jianyang Li, Chunming Dong, Shizheng Xiang, Huiyang Wei, Qiliang Lai, Guangshan Wei, Linfeng Gong, Zhaobin Huang, Donghui Zhou, Guangyi Wang, Zongze Shao","doi":"10.1093/ismeco/ycae133","DOIUrl":"https://doi.org/10.1093/ismeco/ycae133","url":null,"abstract":"Transport of organic matter (OM) occurs widely in the form of animal and plant detritus in global oceans, playing a crucial role in global carbon cycling. While wood- and whale-falls have been extensively studied, the in situ process of OM remineralization by microorganisms remains poorly understood particularly in pelagic regions on a global scale. Here, enrichment experiments with animal tissue or plant detritus were carried out in three deep seas for 4-12 months using the deep-sea in situ incubators. We then performed community composition analyses as well as metagenomic and metatranscriptomic analyses. The results revealed strikingly similar microbial assemblages responsible for decomposing animal and plant detritus. Genes encoding peptidases and glucoside hydrolases were highly abundant and actively transcribed in OM enrichments, which confirmed the roles of these enriched microbial assemblages in organic decomposition. Marinifilaceae, Desulfocapsaceae, Spirochaetaceae, and o-Peptostreptococcales were found to potentially contribute to nitrogen fixation. These core bacteria, acting as cosmopolitan anaerobes in decomposing fast-sinking particulate OM, may have been underestimated in terms of their role in deep-sea microbial-mediated biogeochemical cycles during conventional sampling and diversity survey.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae133"},"PeriodicalIF":5.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High quality Bathyarchaeia MAGs from lignocellulose-impacted environments elucidate metabolism and evolutionary mechanisms. 来自木质纤维素影响环境的高质量深海古菌mag阐明了代谢和进化机制。

IF 5.1

ISME communications Pub Date : 2024-12-10 eCollection Date: 2024-01-01 DOI: 10.1093/ismeco/ycae156

Camilla Lothe Nesbø, Ilya Kublanov, Minqing Yang, Anupama Achal Sharan, Torsten Meyer, Elizabeth A Edwards

{"title":"High quality Bathyarchaeia MAGs from lignocellulose-impacted environments elucidate metabolism and evolutionary mechanisms.","authors":"Camilla Lothe Nesbø, Ilya Kublanov, Minqing Yang, Anupama Achal Sharan, Torsten Meyer, Elizabeth A Edwards","doi":"10.1093/ismeco/ycae156","DOIUrl":"https://doi.org/10.1093/ismeco/ycae156","url":null,"abstract":"The archaeal class Bathyarchaeia is widely and abundantly distributed in anoxic habitats. Metagenomic studies have suggested that they are mixotrophic, capable of CO2 fixation and heterotrophic growth, and involved in acetogenesis and lignin degradation. We analyzed 35 Bathyarchaeia metagenome-assembled genomes (MAGs), including the first complete circularized MAG (cMAG) of the Bathy-6 subgroup, from the metagenomes of three full-scale pulp and paper mill anaerobic digesters and three laboratory methanogenic enrichment cultures maintained on pre-treated poplar. Thirty-three MAGs belong to the Bathy-6, lineage while two are from the Bathy-8 lineage. In our previous analysis of the microbial community in the pulp mill digesters, Bathyarchaeia were abundant and positively correlated to hydrogenotrophic and methylotrophic methanogenesis. Several factors likely contribute to the success of the Bathy-6 lineage compared to Bathy-8 in the reactors. The Bathy-6 genomes are larger than those of Bathy-8 and have more genes involved in lignocellulose degradation, including carbohydrate-active enzymes not present in the Bathy-8. Bathy-6 also shares the Bathyarchaeal O-demethylase system recently identified in Bathy-8. All the Bathy-6 MAGs had numerous membrane-associated pyrroloquinoline quinone-domain proteins that we suggest are involved in lignin modification or degradation, together with Radical-S-adenosylmethionine (SAM) and Rieske domain proteins, and AA2, AA3, and AA6-family oxidoreductases. We also identified a complete B12 synthesis pathway and a complete nitrogenase gene locus. Finally, comparative genomic analyses revealed that Bathyarchaeia genomes are dynamic and have interacted with other organisms in their environments through gene transfer to expand their gene repertoire.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"4 1","pages":"ycae156"},"PeriodicalIF":5.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral niche-partitioning: comparative genomics of giant viruses across environmental gradients in a high Arctic freshwater-saltwater lake. 病毒生态位划分：北极淡水咸水湖高海拔环境梯度巨型病毒的比较基因组学。

IF 5.1

ISME communications Pub Date : 2024-12-08 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae155

Thomas M Pitot, Catherine Girard, Josephine Z Rapp, Vincent Somerville, Alexander I Culley, Warwick F Vincent, Sylvain Moineau, Simon Roux

{"title":"Viral niche-partitioning: comparative genomics of giant viruses across environmental gradients in a high Arctic freshwater-saltwater lake.","authors":"Thomas M Pitot, Catherine Girard, Josephine Z Rapp, Vincent Somerville, Alexander I Culley, Warwick F Vincent, Sylvain Moineau, Simon Roux","doi":"10.1093/ismeco/ycae155","DOIUrl":"10.1093/ismeco/ycae155","url":null,"abstract":"Giant viruses (GVs; Nucleocytoviricota) impact the biology and ecology of a wide range of eukaryotic hosts, with implications for global biogeochemical cycles. Here, we investigated GV niche separation in highly stratified Lake A at the northern coast of Ellesmere Island, Nunavut, Canada. This lake is composed of a layer of ice-covered freshwater that overlies saltwater derived from the ancient Arctic Ocean, and it therefore provides a broad gradient of environmental conditions and ecological habitats, each with a distinct protist community and rich assemblages of associated GVs. The upper layer (mixolimnion) had measurable light and oxygen, and contained diverse GVs linked to photosynthetic protists, indicating adaptation to surface biotic and abiotic conditions. In contrast, the saline lower layer (monimolimnion), lacking oxygen and light, hosted GVs associated with predicted heterotrophic protists, some of which are known for a predatory lifestyle, and with several viral genes suggesting adaptation to deep-water anaerobic conditions. Our observations underscore the coupling between physical and chemical gradients, microeukaryotes and their associated GVs in Lake A, and provide insight into the potential for GVs to directly and indirectly impact host metabolism. There were similarities between the genetic composition of GVs and the metabolic processes of their potential hosts, implying co-evolution and niche-adaptation within the lake habitats. Notably, we found a greater presence of viral rhodopsins in deeper water layers, suggesting an evolutionary relationship with potential hosts capable of supplementing their energetic needs to thrive in low energy, anoxic conditions.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae155"},"PeriodicalIF":5.1,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0