ISME communications最新文献_第2页

Correction to: Hydrogen-independent CO₂ reduction dominates methanogenesis in five temperate lakes that differ in trophic states. 更正：在营养状态不同的五个温带湖泊中，不依赖氢的二氧化碳减少在甲烷生成中占主导地位。

IF 5.1

ISME communications Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf082

引用次数: 0

Coevolution and cross-infection patterns between viruses and their host methanogens in paddy soils. 水稻土中病毒及其宿主产甲烷菌的协同进化和交叉感染模式。

IF 5.1

ISME communications Pub Date : 2025-05-23 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf088

Xingjie Wu, Ye Liu, Zhibin He, Xi Zhou, Werner Liesack, Jingjing Peng

{"title":"Coevolution and cross-infection patterns between viruses and their host methanogens in paddy soils.","authors":"Xingjie Wu, Ye Liu, Zhibin He, Xi Zhou, Werner Liesack, Jingjing Peng","doi":"10.1093/ismeco/ycaf088","DOIUrl":"10.1093/ismeco/ycaf088","url":null,"abstract":"Methanogens play a critical role in global methane (CH4) emissions from rice paddy ecosystems. Through the integration of metagenomic analysis and meta-analysis, we constructed a CRISPR spacer database comprising 14 475 spacers derived from 351 methanogenic genomes. This enabled the identification of viruses targeting key methanogenic families prevalent in rice paddies, including Methanosarcinaceae, Methanotrichaceae, Methanobacteriaceae, Methanocellaceae, and Methanomassiliicoccaceae. We identified 419 virus-host linkages involving 56 methanogenic host species and 189 viruses, spanning the families Straboviridae, Salasmaviridae, Kyanoviridae, Herelleviridae, and Demerecviridae, along with 126 unclassified viral entities. These findings highlight a virome composition that is markedly distinct from those observed in gut environments. Cross-infection patterns were supported by the presence of specific viruses predicted to infect multiple closely related methanogenic species. Evidence for potential virus-host coevolution was observed in 24 viruses encoding anti-CRISPR proteins, likely facilitating evasion of host CRISPR-mediated immunity. Collectively, this study reveals a complex and dynamic network of virus-host interactions shaping methanogen communities in rice paddy ecosystems.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf088"},"PeriodicalIF":5.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic evaluation of protein extraction for metaproteomic analysis of marine sediment with high clay content. 高粘土含量海洋沉积物元蛋白质组学分析中蛋白质提取的系统评价。

IF 5.1

ISME communications Pub Date : 2025-05-22 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf074

Anne Ostrzinski, Benoit J Kunath, André Rodrigues Soares, Cédric C Laczny, Rashi Halder, Jens Kallmeyer, Rolando di Primio, Paul Wilmes, Alexander J Probst, Anke Trautwein-Schult, Dörte Becher

{"title":"Systematic evaluation of protein extraction for metaproteomic analysis of marine sediment with high clay content.","authors":"Anne Ostrzinski, Benoit J Kunath, André Rodrigues Soares, Cédric C Laczny, Rashi Halder, Jens Kallmeyer, Rolando di Primio, Paul Wilmes, Alexander J Probst, Anke Trautwein-Schult, Dörte Becher","doi":"10.1093/ismeco/ycaf074","DOIUrl":"10.1093/ismeco/ycaf074","url":null,"abstract":"Marine sediments harbor extremely diverse microbial communities that contribute to global biodiversity and play an essential role in the functioning of ecosystems. However, the metaproteome of marine sediments is still poorly understood. The extraction of proteins from environmental samples is still a challenge, especially from marine sediments, due to the complexity of the matrix. Therefore, methods for protein extraction from marine sediments need to be improved. To develop an effective workflow for protein extraction for clayey sediments, we compared, combined and enhanced different protein extraction methods. The workflow presented here includes blocking of protein binding sites on sediment particles with high concentrations of amino acids, effective cell lysis by ultrasonic capture, electro-elution, and simultaneous fractionation of proteins. To test the protocol's efficacy, we added Escherichia coli cells to sediment samples before protein extraction. By using our refined workflow, we were able to identify a comparable number of E. coli proteins from the supplemented sediment to those from pure E. coli cultures. This new protocol will enable future studies to identify active players in clay-rich marine sediments and accurately determine functional biodiversity based on their respective protein complements.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf074"},"PeriodicalIF":5.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12192440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting defense strategies of oligotrophs and copiotrophs revealed by single-cell-resolved virus-host pairing of freshwater bacteria. 淡水细菌单细胞分解病毒-宿主配对揭示寡养和共养生物防御策略的对比。

IF 5.1

ISME communications Pub Date : 2025-05-21 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf086

Yusuke Okazaki, Yohei Nishikawa, Ryota Wagatsuma, Haruko Takeyama, Shin-Ichi Nakano

{"title":"Contrasting defense strategies of oligotrophs and copiotrophs revealed by single-cell-resolved virus-host pairing of freshwater bacteria.","authors":"Yusuke Okazaki, Yohei Nishikawa, Ryota Wagatsuma, Haruko Takeyama, Shin-Ichi Nakano","doi":"10.1093/ismeco/ycaf086","DOIUrl":"10.1093/ismeco/ycaf086","url":null,"abstract":"Characterizing virus-host pairs and the infection state of individual cells is the major technical challenge in microbial ecology. We addressed these challenges using state-of-the-art single-cell genome technology (SAG-gel) combined with extensive metagenomic datasets targeting the bacterial and viral communities in Lake Biwa. From two water layers and two seasons, we obtained 862 single-cell amplified genomes (SAGs), including 176 viral (double-stranded DNA phage) contigs, which identified novel virus-host pairs involving dominant freshwater lineages. The viral infection rate, estimated by mapping the individual SAG's raw reads to viral contigs, showed little variation among samples (12.1%-18.1%) but significant variation in host taxonomy (4.2%-65.3%), with copiotrophs showing higher values than oligotrophs. The high infection rates of copiotrophs were attributed to collective infection by diverse viruses, suggesting weak density-dependent virus-host selection, presumably due to their nonpersistent interactions with viruses resulting from fluctuating abundance. In contrast, the low infection rates of oligotrophs supported the idea that their codominance with viruses is achieved by genomic microdiversification, which diversifies the virus-host specificity, sustained by their large population size and persistent density-dependent fluctuating selection. Notably, we discovered viruses infecting CL500-11, the dominant bacterioplankton lineage in deep freshwater lakes worldwide. These viruses showed extremely high read coverages in cellular and virion metagenomes but were detected in <1% of host cells, suggesting a low infection rate and high burst size. Overall, we revealed highly diverse virus-host interactions within and between host lineages that were overlooked at the metagenomic resolution.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf086"},"PeriodicalIF":5.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial and fungal composition and exometabolites control the development and persistence of soil water repellency. 细菌和真菌的组成和外代谢产物控制着土壤拒水性的发展和持续。

IF 5.1

ISME communications Pub Date : 2025-05-20 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf084

Emily N Boak, Benjamin P Bowen, Katherine B Louie, Trent R Northen, Marie E Kroeger

{"title":"Bacterial and fungal composition and exometabolites control the development and persistence of soil water repellency.","authors":"Emily N Boak, Benjamin P Bowen, Katherine B Louie, Trent R Northen, Marie E Kroeger","doi":"10.1093/ismeco/ycaf084","DOIUrl":"10.1093/ismeco/ycaf084","url":null,"abstract":"Soil water repellency (SWR), the reduced affinity of soil for water, is a phenomenon that affects soils globally. With worsening climate change, SWR is expected to increase emphasizing the need to understand the mechanisms driving SWR development and persistence. The importance of the soil microbes in SWR has been postulated for decades, but limited research has been conducted into whole-community interactions and the role of community metabolic activity. To address this gap in knowledge, we investigated the direct effect of microbial community composition, activity, and diversity, as well as their associated metabolites on the development and persistence of SWR by inoculating microcosms containing model soils with 15 different microbial communities and quantified respiration and SWR over time. Six communities that consistently produced either a hydrophobic or hydrophilic phenotype were characterized using metagenomics and metabolomics to determine the impact of microbial and metabolite composition and diversity on SWR. We identified several bacterial genera with significant changes in abundance between SWR phenotypes including Nocardiopsis and Kocuria in hydrophilic and Streptomyces and Cutibacterium in hydrophobic. We discovered that hydrophilic communities were more positively connected when compared to hydrophobic communities, which could be due to an increase in defense mechanism genes. Additionally, we identified specific metabolites associated with hydrophilic and hydrophobic phenotypes including an increase in the osmolyte ectoine in hydrophilic and an increase in plant-derived decomposition products in hydrophobic communities. Finally, our research suggests that fungi, previously thought to cause hydrophobicity, may actually contribute to hydrophilicity through their preferential consumption of hydrophobic compounds.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf084"},"PeriodicalIF":5.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In a sea of microbes, eddy events trigger diatom export in the Sargasso Sea. 在微生物的海洋中，涡旋事件触发了马尾藻海的硅藻输出。

IF 5.1

ISME communications Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf083

Marc Alec Fontánez Ortiz, Francesca De Martini, Susanne Neuer

{"title":"In a sea of microbes, eddy events trigger diatom export in the Sargasso Sea.","authors":"Marc Alec Fontánez Ortiz, Francesca De Martini, Susanne Neuer","doi":"10.1093/ismeco/ycaf083","DOIUrl":"10.1093/ismeco/ycaf083","url":null,"abstract":"Sinking particles are important conduits of organic carbon from the euphotic zone to the deep ocean, but their origin and community composition are still a matter of investigation. Events in the northwestern Sargasso Sea, such as winter convective mixing, summer stratification, and mesoscale eddies, affect the vertical and temporal composition and abundance of pelagic and particle-attached microorganisms. We sampled the euphotic zone and collected sinking particles using shallow traps near the Bermuda Atlantic Time-series Study site during the spring and summer of 2012 to assess eddy-driven impact on microbial communities. In the spring, we sampled a cyclonic eddy, while in the summer, we targeted both the center and edge of an anticyclonic eddy. Prokaryotic and photoautotrophic (plastid and cyanobacteria) communities were analyzed using V4-V5 amplicons of the 16S rRNA gene. Community and clustering analysis of prokaryotes revealed a clear separation between seawater and particles samples. However, the same was not observed for photoautotrophs. Indicator species analysis showed that small phytoplankton taxa dominated particle communities. Interestingly, differential abundance analyses revealed that the large centric diatom, Rhizosolenia, generally rare in the oligotrophic Sargasso Sea, was enriched in the photoautotrophic communities of sinking particles collected in the center of the anticyclonic eddy with unusual upwelling due to eddy-wind interactions. We hypothesize that the steady contribution of small-celled phytoplankton to particle flux is punctuated by pulses of production and flux of larger-sized phytoplankton in response to episodic eddy upwelling events and can lead to higher export of particulate organic matter during the summer.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf083"},"PeriodicalIF":5.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensor-aided isolation of anaerobic arsenic-methylating bacteria from soil. 生物传感器辅助分离土壤中厌氧砷甲基化细菌。

IF 5.1

ISME communications Pub Date : 2025-05-09 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf081

Hugo Sallet, Luna Kaiser, Matteo Titus, Marion Calvo, Nicolas Jacquemin, Karin Lederballe Meibom, Rizlan Bernier-Latmani

{"title":"Biosensor-aided isolation of anaerobic arsenic-methylating bacteria from soil.","authors":"Hugo Sallet, Luna Kaiser, Matteo Titus, Marion Calvo, Nicolas Jacquemin, Karin Lederballe Meibom, Rizlan Bernier-Latmani","doi":"10.1093/ismeco/ycaf081","DOIUrl":"10.1093/ismeco/ycaf081","url":null,"abstract":"Microbial methylation of arsenic impacts both the toxicity and fate of this environmental contaminant and is an important component of its biogeochemical cycle. This transformation occurs in flooded paddy fields where soil microorganisms can produce dimethylated arsenic, which causes the straighthead disease in rice. The responsible anaerobic microorganisms have remained elusive because their isolation is laborious, especially as the active methylators cannot be rapidly screened. Here, we introduce a novel approach to specifically target these microorganisms. This approach is based on a high-throughput isolation technique involving microfluidic encapsulation, fluorescence-activated cell sorting, and biosensor-aided screening of microbial function. Using this method, we isolated two arsenic-methylating anaerobes from a paddy soil. This approach has the potential to rapidly obtain novel isolates. For instance, we show that one isolate actively methylates arsenate (AsV), a previously unknown phenotype in anaerobes.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf081"},"PeriodicalIF":5.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal dynamics and biogeography of sympagic and planktonic photosynthetic microbial eukaryotes during the under-ice Arctic bloom. 北极冰下水华期间共生和浮游光合微生物真核生物的时间动态和生物地理。

IF 5.1

ISME communications Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf075

Clarence Wei Hung Sim, Catherine Gérikas Ribeiro, Florence Le Gall, Ian Probert, Priscilla Gourvil, Connie Lovejoy, Daniel Vaulot, Adriana Lopes Dos Santos

{"title":"Temporal dynamics and biogeography of sympagic and planktonic photosynthetic microbial eukaryotes during the under-ice Arctic bloom.","authors":"Clarence Wei Hung Sim, Catherine Gérikas Ribeiro, Florence Le Gall, Ian Probert, Priscilla Gourvil, Connie Lovejoy, Daniel Vaulot, Adriana Lopes Dos Santos","doi":"10.1093/ismeco/ycaf075","DOIUrl":"10.1093/ismeco/ycaf075","url":null,"abstract":"Photosynthetic microbial eukaryotes play a pivotal role as primary producers in the Arctic Ocean, where seasonal blooms within and below the ice are crucial phenomena, contributing significantly to global primary production and biogeochemical cycling. In this study, we investigated the taxonomic composition of sympagic algae and phytoplankton communities during the Arctic under-ice spring bloom using metabarcoding of the 18S rRNA gene. Samples were obtained from three size fractions over a period of nearly three months at an ice camp deployed on landfast ice off the coast of Baffin Island as part of the Green Edge project. We classified the major sympagic and phytoplankton taxa found in this study into biogeographical categories using publicly available metabarcoding data from more than 2800 oceanic and coastal marine samples. This study demonstrated the temporal succession of taxonomic groups during the development of the under-ice bloom, illustrated by an overall transition from polar to polar-temperate taxa, particularly in the smallest size fraction. Overlooked classes such as Pelagophyceae (genera Plocamiomonas and Ankylochrysis), Bolidophyceae (Parmales environmental clade 2), and Cryptophyceae (Baffinella frigidus) might play a greater role than anticipated within the pico-sized communities in and under the ice pack during the pre-bloom period. Finally, we emphasize the importance of microdiversity, taking the example of B. frigidus, for which two ecotypes linked to pelagic and sea ice environments have been identified.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf075"},"PeriodicalIF":5.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular complexity of the differential growth of freshwater diatoms along pH gradients. 淡水硅藻沿pH梯度差异生长的分子复杂性。

IF 5.1

ISME communications Pub Date : 2025-05-06 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf078

Xènia Rodríguez-Miret, Marisol Felip, Eric Pelletier, Jordi Catalan

{"title":"Molecular complexity of the differential growth of freshwater diatoms along pH gradients.","authors":"Xènia Rodríguez-Miret, Marisol Felip, Eric Pelletier, Jordi Catalan","doi":"10.1093/ismeco/ycaf078","DOIUrl":"10.1093/ismeco/ycaf078","url":null,"abstract":"Diatoms originated in marine waters, and many clades have invaded fresh waters, radiating throughout the continental aquatic environments. pH gradients have been a primary axis of species differentiation, from which environmental assessments have taken advantage using diatoms as bioindicators. However, a comprehensive view of the molecular variation underlying the diatom sensitivity to pH is missing. This study used 12 freshwater diatom strains across a broad phylogenetic range within raphid pennate clades and 3 distinct environmental pH conditions, pH 4.7, 7.0, and 8.2, for a common garden experiment. The transcriptomic analysis showed that environmental pH variation regulated many molecular processes and biological functions, especially those involved in biosynthesis and transport. Despite sharing many known functions, strains responded to pH changes in a highly idiosyncratic manner. Such specificity in the physiological response to pH aligns with the considerably divergent genetic backgrounds observed among the 12 diatom strains. This variation was likely shaped by different evolutionary trajectories in adaptive molecular landscapes, which were probably already differentiated in the initial marine environment and subjected to varying pH selection pressures in the complex chemical mosaic of inland waters. Overall, our results indicate that continental pH selection pressures do not determine a necessarily unique adaptive physiological response in diatoms, but instead allow for multiple adaptive solutions built on the evolutionary historical background and inland contingencies. Therefore, specific studies on the identified plastic responses to pH are needed to assess their adaptive function across clades.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf078"},"PeriodicalIF":5.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil viral communities shifted significantly after wildfire in chaparral and woodland habitats. 灌木林和林地野火后土壤病毒群落发生了显著变化。

IF 5.1

ISME communications Pub Date : 2025-05-06 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf073

Sara E Geonczy, Anneliek M Ter Horst, Joanne B Emerson

{"title":"Soil viral communities shifted significantly after wildfire in chaparral and woodland habitats.","authors":"Sara E Geonczy, Anneliek M Ter Horst, Joanne B Emerson","doi":"10.1093/ismeco/ycaf073","DOIUrl":"10.1093/ismeco/ycaf073","url":null,"abstract":"Increased wildfire activity warrants more research into fire-driven biotic changes in soil, including soil viral communities, given the roles of soil microbes in organic matter decomposition, nutrient cycling, and post-fire recovery. Leveraging viral size-fraction metagenomes (viromes), here we studied viral community responses to wildfire in woodland and chaparral soils at five timepoints over 1 year following the California LNU Complex wildfire. We also compared post-fire samples to unburned controls at the final three timepoints and leveraged published viromes from the same sites nine months before the fire as pre-burn controls. Viral community composition differed significantly in burned samples compared to controls from both habitats, as did soil chemistry and prokaryotic communities (16S rRNA gene amplicons). Viromic DNA yields (a proxy for viral particle abundances) indicated initial viral biomass reductions due to the fire, but a return to baseline abundances (indistinguishable from controls) within five months. Fire-associated habitat filtering was further indicated by a comparison to the PIGEON viral \"species\" (viral operational taxonomic unit (vOTU)) reference database, with vOTUs from a burned conifer forest representing 19%-31% of PIGEON vOTUs detected in the burned habitats but only 0.6%-6% in controls. Together, these results indicate significant changes in soil viral communities due to wildfire, attributable at least in part to concomitant changes in their prokaryotic host communities and soil physicochemistry.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf073"},"PeriodicalIF":5.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0