iRadiologyPub Date : 2026-07-02Epub Date: 2026-06-23DOI: 10.1002/ird3.70084
Jiahui Wang, Jun Wang, Shu Gao, Kun Qian, Qihang Ding, Zhen Cheng
{"title":"Advances in Fluorescent Probes Targeting Senescence-Associated β-Galactosidase for Senescence Imaging","authors":"Jiahui Wang, Jun Wang, Shu Gao, Kun Qian, Qihang Ding, Zhen Cheng","doi":"10.1002/ird3.70084","DOIUrl":"https://doi.org/10.1002/ird3.70084","url":null,"abstract":"<p>Aging is a systemic biological process that spans multiple levels, including molecules, cells, tissues, and organs. Cellular senescence is regarded as one of the key mechanisms driving functional decline, chronic inflammation, and the onset and progression of age-related diseases. Among the numerous biomarkers of senescence, senescence-associated β-galactosidase (SA-β-gal) has become one of the most widely used classical indicators in cellular senescence research. However, conventional X-gal staining and lysate-based assays largely rely on endpoint, ex vivo, or semi-quantitative readouts, making it difficult to meet the growing demand for real-time, in situ, dynamic, and highly sensitive visualization of senescent states in live cells, tissue sections, and living organisms. The development of fluorescence imaging technologies, particularly near-infrared fluorescence imaging, has provided a new toolkit for detecting SA-β-gal activity. Fluorescent probes containing a β-D-galactose unit can be activated by β-galactosidase, resulting in the recovery or change of fluorescence signals. This review systematically summarizes recent advances in senescence imaging targeting SA-β-gal, including turn-on probes, ratiometric probes, AIE-based probes, and nanoprobes. In particular, we highlight strategies to improve the specificity of SA-β-gal imaging, enhance the ability to distinguish SA-β-gal activity in senescent cells from that in non-senescent tumor cells, and further discuss the challenges and opportunities in this field.</p>","PeriodicalId":73508,"journal":{"name":"iRadiology","volume":"4 3","pages":"192-205"},"PeriodicalIF":2.4,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ird3.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iRadiologyPub Date : 2026-07-02Epub Date: 2026-06-15DOI: 10.1002/ird3.70081
Jian Rong, Chunyu Zhao, Xin Zhou, Jiahui Chen, Yabiao Gao, Zhendong Song, Yinlong Li, Zijing Li, Steven H. Liang
{"title":"Development of a Novel 18F-Labeled Radioligand for Imaging Translocator Protein With Positron Emission Tomography","authors":"Jian Rong, Chunyu Zhao, Xin Zhou, Jiahui Chen, Yabiao Gao, Zhendong Song, Yinlong Li, Zijing Li, Steven H. Liang","doi":"10.1002/ird3.70081","DOIUrl":"https://doi.org/10.1002/ird3.70081","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Translocator protein 18 kDa (TSPO) is a well-established biomarker of neuroinflammation and is implicated in the pathophysiology of various neurodegenerative diseases, including Alzheimer's disease (AD). Despite existing radioligands, there remains a critical need for novel TSPO-targeted positron emission tomography (PET) tracers with improved binding specificity, higher brain uptake, and favorable pharmacokinetics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We designed and synthesized a class of novel TSPO ligand candidates. Following in vitro binding affinity screening, the lead <b>Compound 3</b> (TSPO-1118) was radiolabeled with fluorine-18. [<sup>18</sup>F]<b>3</b> ([<sup>18</sup>F]TSPO-1118) was evaluated in two transgenic AD mouse models (3xTg and APP/PS1) and compared to age-matched wild-type (WT) controls. Biodistribution and radiometabolite analyses were conducted in CD-1 mice to assess brain penetration and metabolic stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><b>Compound 3</b> (TSPO-1118) demonstrated superior binding affinity for TSPO (<i>K</i><sub><i>i</i></sub> = 2.3 nM), outperforming both other analogs in the series and the benchmark tracer GE180. [<sup>18</sup>F]<b>3</b> ([<sup>18</sup>F]TSPO-1118) exhibited significantly higher brain uptake in both 3xTg and APP/PS1 mice relative to WT controls, including the cortex, thalamus, cerebellum, and hippocampus (<i>p</i> < 0.05 to < 0.01, Student's two-tailed <i>t</i>-test), consistent with elevated TSPO expression in disease models. The tracer also displayed favorable brain kinetics, high specific binding, and metabolic stability in vivo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>[<sup>18</sup>F]<b>3</b> ([<sup>18</sup>F]TSPO-1118) represents a promising next-generation PET radioligand for imaging TSPO with high affinity, robust brain uptake, and specificity in preclinical models of neurodegeneration. Its favorable profile supports further translational development toward clinical application in neuroinflammatory and neurodegenerative disorders.</p>\u0000 </section>\u0000 </div>","PeriodicalId":73508,"journal":{"name":"iRadiology","volume":"4 3","pages":"261-272"},"PeriodicalIF":2.4,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ird3.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iRadiologyPub Date : 2026-07-02Epub Date: 2026-06-10DOI: 10.1002/ird3.70076
Yonghao Li, Aiyan Ji, Qihang Ding, Hongyue Lou, Zhen Cheng
{"title":"c-Met-Targeted Imaging Agents: Progress and Challenges","authors":"Yonghao Li, Aiyan Ji, Qihang Ding, Hongyue Lou, Zhen Cheng","doi":"10.1002/ird3.70076","DOIUrl":"https://doi.org/10.1002/ird3.70076","url":null,"abstract":"<p>Cellular-mesenchymal epithelial transition factor (c-Met), a receptor tyrosine kinase, participates in various human physiological processes, including cell proliferation, embryonic development, and tissue regeneration via the hepatocyte growth factor (HGF)/c-Met signaling pathway. Abnormal activation of this pathway is associated with tumor growth and treatment resistance. Previous work has demonstrated that c-Met is overexpressed in various solid tumors, making it a potential therapeutic target. The development of diagnostic and therapeutic agents targeting c-Met is also of considerable interest. In this review, we provide an overview of the current progress with c-Met-targeted probes that use HGF ligands, antibodies, peptides, and small molecules for noninvasive imaging with techniques such as MRI, PET, SPECT, and optical imaging. We also summarize the design strategies and imaging effects used for tumor diagnosis. Through these insights, we aim to facilitate the further advancement of novel c-Met-targeted imaging agents, thereby enhancing the precision of tumor diagnosis and evaluation of drug efficacy.</p>","PeriodicalId":73508,"journal":{"name":"iRadiology","volume":"4 3","pages":"219-245"},"PeriodicalIF":2.4,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ird3.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
