Advances in medical sciences最新文献_第6页

Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration 骨髓来源的非常小的胚胎样干细胞与凝血酶激活的富血小板血浆在子宫内膜再生中的治疗潜力。

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-05-24 DOI: 10.1016/j.advms.2025.05.002

Jeevitaa Kshersagar , Akshay A. Kawale , Leena R. Chaudhari , Mrunal N. Damle , Rakesh Kumar Sharma , Meghnad G. Joshi

{"title":"Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration","authors":"Jeevitaa Kshersagar , Akshay A. Kawale , Leena R. Chaudhari , Mrunal N. Damle , Rakesh Kumar Sharma , Meghnad G. Joshi","doi":"10.1016/j.advms.2025.05.002","DOIUrl":"10.1016/j.advms.2025.05.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Endometrium, a dynamic tissue undergoing cyclic changes, plays a pivotal role in reproductive health. Disruptions in its structure and function can lead to infertility and pregnancy complications. Stem cell-based therapies, including very small embryonic-like stem cells (VSELS) and platelet-rich plasma (PRP), have shown promise in tissue regeneration.</div></div><div><h3>Methods</h3><div>We investigated the efficacy of bone marrow-derived VSELS combined with thrombin-activated PRP (aPRP) for endometrial regeneration in a murine model of disturbed endometrium (DE). Characterization of bone marrow very small embryonic like stem cells (BM VSELS) revealed pluripotency markers and negative expression for CD34, Tie-2, Thy, CD133, CD90, and delta-like protein (DLK).</div></div><div><h3>Results</h3><div>Transplantation of BM VSELS-aPRP resulted in their engraftment in the endometrium, with enhanced endometrial thickness, collagen reformation, and improved marker expression compared to controls. Immunohistochemical analysis demonstrated increased expression of α-SMA, CK-18, CK-19, E-Cad, Cla-1, CX-40, and ZO-1 in the transplant group. Pregnancy outcomes improved in the BM VSELS-aPRP group, with successful conception and delivery of healthy pups.</div></div><div><h3>Conclusion</h3><div>This study highlights the regenerative potential of BM VSELS-aPRP for endometrial repair and suggests a novel therapeutic approach for endometrial disorders and infertility.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 255-265"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intensification of diuretic therapy in pulmonary hypertension – a retrospective evaluation of hemodynamic and functional changes 加强利尿剂治疗肺动脉高压-血流动力学和功能改变的回顾性评估。

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 DOI: 10.1016/j.advms.2025.09.006

Adam Rafałowicz, Jakub Mierzejewski, Łukasz Czuba, Anna Lisowska, Małgorzata Knapp, Karol Kamiński, Katarzyna Ptaszyńska

{"title":"Intensification of diuretic therapy in pulmonary hypertension – a retrospective evaluation of hemodynamic and functional changes","authors":"Adam Rafałowicz, Jakub Mierzejewski, Łukasz Czuba, Anna Lisowska, Małgorzata Knapp, Karol Kamiński, Katarzyna Ptaszyńska","doi":"10.1016/j.advms.2025.09.006","DOIUrl":"10.1016/j.advms.2025.09.006","url":null,"abstract":"<div><h3>Purpose</h3><div>Diuretics are first-line medication in the management of volume overload in pulmonary hypertension (PH). Their impact on the hemodynamic and functional parameters has not been studied. This study analyzes the impact of intensified diuretic therapy on patients deterioration.</div></div><div><h3>Methods</h3><div>The retrospective analysis included 38 patients who were divided into Group 1 (n = 24, no change in the equivalent diuretic dose or treatment without diuretics) and Group 2 (n = 14, increase in the equivalent diuretic dose). Measurements at baseline and after 6 months included echocardiographic parameters, 6-min walk test distance (6MWTd), and N-terminal prohormone of brain natriuretic peptide (NT-proBNP).</div></div><div><h3>Results</h3><div>In the initial assessment of the studied parameters, Group 2 had a significantly greater inferior vena cava diameter (IVCd) and right ventricle internal diameter in diastole (RVIDd), trends towards greater right atrium area (RAA), lower tricuspid annular plane systolic excursion (TAPSE), and greater NT-proBNP values compared to Group 1. Over the 6-month follow-up period, Group 1 had a significant increase in IVCd, systolic pulmonary artery pressure (sPAP), significant decrease in TAPSE/sPAP ratio and trends towards increased mean pulmonary artery pressure (mPAP) and 6MWTd values. Group 2, on the other hand, had no significant worsening, which reflects a stabilizing impact of intensified diuretic therapy.</div></div><div><h3>Conclusion</h3><div>Intensified diuretic therapy in patients with PH and fluid overload appears to stabilize exercise capacity and prevent deterioration of cardiac function over a 6-month period.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 382-391"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to Letter to the Editor on “Structured team response and sex-based mortality gaps in acute pulmonary embolism: Conflicting evidence and future directions” 对致编辑的关于“急性肺栓塞的结构化团队反应和基于性别的死亡率差距：相互矛盾的证据和未来方向”的回复。

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-11-07 DOI: 10.1016/j.advms.2025.10.005

Grzegorz Procyk , Arkadiusz Pietrasik

引用次数: 0

Impact of anti-HLA antibodies on the immunological status of patients with cardiac allograft vasculopathy (CAV) after heart transplantation. hla抗体对心脏移植后同种异体移植物血管病变（CAV）患者免疫状态的影响

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-07-23 DOI: 10.1016/j.advms.2025.07.001

Katarzyna Kowalik, Joanna Was, Katarzyna Kozar-Kaminska, Ilona Minota, Krzysztof Komuda, Aneta Rekawek, Tomasz Zielinski

{"title":"Impact of anti-HLA antibodies on the immunological status of patients with cardiac allograft vasculopathy (CAV) after heart transplantation.","authors":"Katarzyna Kowalik, Joanna Was, Katarzyna Kozar-Kaminska, Ilona Minota, Krzysztof Komuda, Aneta Rekawek, Tomasz Zielinski","doi":"10.1016/j.advms.2025.07.001","DOIUrl":"10.1016/j.advms.2025.07.001","url":null,"abstract":"<p><strong>Purpose: </strong>Heart transplantation (HTx) is an established method of treating patients with end-stage heart failure. Properly selected immunosuppression effectively reduces the risk of acute transplant rejection; however, it may also lead to various side effects including cardiac allograft vasculopathy (CAV), which is one of the most common causes of death in patients during the first year after HTx. There is an urgent need to develop new strategies for monitoring a patient's immune system to modify the immunosuppressive therapy, if needed. The purpose of this study is to compare the immune status of patients with diagnosed CAV + to that of patients without CAV-.</p><p><strong>Material and methods: </strong>We collected blood samples from patients long-term after transplantation: 19 clinically stable patients CAV+ and 19 CAV-. Immunological parameters were assessed by analyzing lymphocyte subpopulations with flow cytometry and detecting the presence of alloantibodies with the use of Luminex technology.</p><p><strong>Results: </strong>Statistical analysis demonstrated significant differences between CAV+ and CAV- patients only within the group of patients with detected alloantibodies (AlloAb+). The most notable observation is that a significantly higher proportion of CAV + AlloAb + patients demonstrated the CD4<sup>+</sup> T lymphocyte population expressing IL2 compared to CAV-AlloAb + patients. Similarly, in these groups, we observed a prevalence of CD8<sup>+</sup> lymphocytes expressing IFNγ+ and IL2+TNFα+IFNγ+.</p><p><strong>Conclusion: </strong>Our study provides new evidence that the presence of alloantibodies in the bloodstream of patients after HTx impacts T cells activation and subsequent cytokine production. These data may facilitate establishing parameters that could help identify patients with a higher risk of CAV progression.</p>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":" ","pages":"291-300"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen (rs3134646) and ARHGAP15 (rs4662344) genetic variants may predispose to colonic diverticulosis 胶原蛋白（rs3134646）和ARHGAP15 （rs4662344）基因变异可能易患结肠憩室病

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-04-22 DOI: 10.1016/j.advms.2025.04.001

Piotr Nehring , Grzegorz Placha , Adam Przybyłkowski

{"title":"Collagen (rs3134646) and ARHGAP15 (rs4662344) genetic variants may predispose to colonic diverticulosis","authors":"Piotr Nehring , Grzegorz Placha , Adam Przybyłkowski","doi":"10.1016/j.advms.2025.04.001","DOIUrl":"10.1016/j.advms.2025.04.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Colonic diverticulosis is a common condition in older adults in the entire world. Besides environmental factors, there are emerging data on underlying genetic predisposition to diverticula formation. The study aimed to identify genetic factors associated with colonic diverticulosis.</div></div><div><h3>Patients and methods</h3><div>The study involved 323 patients, 134 with colonic diverticulosis, and 189 healthy controls. In all study participants the following genetic variants were assessed using RT-PCR: rs1800587 in <em>IL1</em>, rs16944, and rs2853550 in <em>IL1B</em>, rs3134646, and rs6434304 in <em>COL3A1</em>, rs3771810, and rs3771863 in <em>TACR1</em>, rs4644560 in <em>NK2R</em>, rs4662344 in <em>ARHGAP15</em>, rs67153654 in <em>FAM155A</em>, and rs7848647 in <em>TNFSF15</em>.</div></div><div><h3>Results</h3><div>Both, the allele C of the <em>COL3A1</em> (rs3134646) variant and allele T of the <em>ARHGAP15</em> (rs4662344) variant, were shown to be associated with diverticulosis, compared to healthy controls in co-dominant and recessive models. Both of them were more frequently reported in patients with colonic diverticulosis, compared to healthy controls.</div><div>There were no allelic or genetic associations in patients with diverticulosis or diverticulitis compared to healthy controls, for the following genetic variants: <em>IL1A</em> (rs1800587), <em>IL1B</em> (rs16944, rs2853550), <em>COL3A1</em> (rs6434304), <em>TACR1</em> (rs3771810, rs3771863), <em>NK2R</em> (rs4644560), <em>FAM155A</em> (rs67153654) and <em>TNFSF15</em> (rs7848647).</div></div><div><h3>Conclusions</h3><div>The allele C of <em>COL3A1</em> (rs3134646) and allele T of the <em>ARHGAP15</em> (rs4662344) may predispose to colonic diverticulosis. When viewed in the context of previous studies, these findings suggest that the development of colonic diverticula may be influenced by the involvement and degradation of the extracellular matrix.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 231-236"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro effects of transient receptor potential channel modulators on human tuba smooth muscle 瞬时受体电位通道调节剂对人大号平滑肌的体外作用。

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-11-04 DOI: 10.1016/j.advms.2025.11.001

Ozan Ozturk , Selin Acar-Sahan , Baris Karadas , Mustafa Sengul , Huseyin Yilmaz , Selen Bahceci , Tijen Kaya-Temiz

{"title":"In vitro effects of transient receptor potential channel modulators on human tuba smooth muscle","authors":"Ozan Ozturk , Selin Acar-Sahan , Baris Karadas , Mustafa Sengul , Huseyin Yilmaz , Selen Bahceci , Tijen Kaya-Temiz","doi":"10.1016/j.advms.2025.11.001","DOIUrl":"10.1016/j.advms.2025.11.001","url":null,"abstract":"<div><h3>Purpose</h3><div>There is relatively little data on the presence of Transient Receptor Potential (TRP) channels in human tubal tissue. Our study aimed to evaluate the contraction-relaxation responses of TRPV1 channel modulators in human tuba uterina tissue. We also aimed to investigate the presence of TRPV1 channel proteins by immunohistochemical staining with TRPV1 antibodies in tuba tissue.</div></div><div><h3>Material and method</h3><div>Ten patients with a mean age of 37.7 years who underwent tubal ligation for sterilization were included. Tissue samples were evaluated for <em>in vitro</em> contractility in a tissue bath. Changes in tuba smooth muscle isometric tension were measured in mg contraction. The basal mean tension was considered as 100 and capsaicin responses in the presence or absence of antagonist were expressed as a percentage of the basal mean tension.</div></div><div><h3>Results</h3><div>Concentrations of capsaicin 10<sup>−7</sup>, 10<sup>−6</sup>, 10<sup>−5</sup>, and 10<sup>−4</sup> M; AMG 9810 10<sup>−5</sup> M and 3x10<sup>−5</sup> M; ruthenium red 10<sup>−5</sup> M and 3x10<sup>−5</sup> M were used. Relaxation response was obtained after cumulative capsaicin administration (maximum relaxation by 20 %). This relaxation was partially reversed when capsaicin was applied in the presence of AMG 9810. When capsaicin was applied in the presence of ruthenium red, a complete reversal of relaxation was observed. Immunohistochemical examination of tuba tissue using TRPV1 antibodies revealed moderate to high staining.</div></div><div><h3>Conclusion</h3><div>The presence of TRPV1 channel in human tuba tissue and its relaxant effect were demonstrated for the first time in this study.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 427-435"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive assessment of triclosan-induced toxicity: impacts on zebrafish development, mammalian cell viability and microbial activity 三氯生毒性的综合评估：对斑马鱼发育、哺乳动物细胞活力和微生物活性的影响。

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-09-04 DOI: 10.1016/j.advms.2025.09.001

Piotr Stachurski , Łukasz Kurach , Maryna Khalavka , Maja Ptasiewicz , Wojciech Świątkowski , Renata Żelazowska , Agnieszka Magryś

{"title":"Comprehensive assessment of triclosan-induced toxicity: impacts on zebrafish development, mammalian cell viability and microbial activity","authors":"Piotr Stachurski , Łukasz Kurach , Maryna Khalavka , Maja Ptasiewicz , Wojciech Świątkowski , Renata Żelazowska , Agnieszka Magryś","doi":"10.1016/j.advms.2025.09.001","DOIUrl":"10.1016/j.advms.2025.09.001","url":null,"abstract":"<div><h3>Purpose</h3><div>The aim of the study was to evaluate the toxicity of triclosan in the <em>Danio rerio</em> model and mammalian cells, as well as to assess its antimicrobial and antibiofilm activity against selected bacterial pathogens.</div></div><div><h3>Methods</h3><div>Triclosan toxicity was assessed in <em>Danio rerio</em> embryos in accordance with OECD Test Guideline 236: Fish Embryo Acute Toxicity (FET) Test. Cytotoxicity was evaluated <em>in vitro</em> using the MTT assay on human dermal fibroblasts (BJ) and rat cardiomyoblasts (H9c2). Antimicrobial activity was assessed via broth microdilution method following EUCAST guidelines and antibiofilm effects were evaluated through minimum biofilm inhibitory concentration (MBIC) assays for each tested strain.</div></div><div><h3>Results</h3><div>Triclosan exhibited toxicity towards <em>Danio rerio</em>, reducing their survival rate, heart rate, and locomotor activity. <em>In vitro</em> tests revealed cytotoxicity (LC<sub>50</sub> = 28.44 μg/mL for BJ cells and 25.36 μg/mL for H9c2 cells). Antimicrobial tests demonstrated MIC values ranging from 0.24 μg/mL (<em>Lactobacillus acidophilus</em>) to 3.9 μg/mL (<em>Enterococcus faecalis</em>), with corresponding MBC values up to 7.8 μg/mL. MBIC values ranged from 0.03 to 0.12 μg/mL, confirming potent antibiofilm activity.</div></div><div><h3>Conclusions</h3><div>Triclosan demonstrates high antimicrobial efficacy and inhibits bacterial biofilm formation. However, its toxicity towards aquatic organisms, including <em>Danio rerio</em>, and its cytotoxic effects on mammalian cells raise concerns.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 367-376"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of hepatitis D virus in chronic hepatitis B patients: findings from Poland 慢性乙型肝炎患者中丁型肝炎病毒的流行：来自波兰的调查结果

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-07-25 DOI: 10.1016/j.advms.2025.07.003

Dorota Zarębska-Michaluk , Michał Brzdęk , Krystyna Dobrowolska , Diana Martonik , Anna Parfieniuk-Kowerda , Jakub Janczura , Kinga Brzdęk , Robert Pleśniak , Agnes Piszcz , Robert Flisiak

{"title":"Prevalence of hepatitis D virus in chronic hepatitis B patients: findings from Poland","authors":"Dorota Zarębska-Michaluk , Michał Brzdęk , Krystyna Dobrowolska , Diana Martonik , Anna Parfieniuk-Kowerda , Jakub Janczura , Kinga Brzdęk , Robert Pleśniak , Agnes Piszcz , Robert Flisiak","doi":"10.1016/j.advms.2025.07.003","DOIUrl":"10.1016/j.advms.2025.07.003","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aimed to determine the prevalence of hepatitis B/hepatitis D virus (HBV/HDV) co-infections in eastern Poland.</div></div><div><h3>Materials/methods</h3><div>We included consecutive patients with chronic hepatitis B treated with nucleos(t)ide analogues (NA) in the study, in whom we performed anti-HDV assays verified by molecular testing.</div></div><div><h3>Results</h3><div>The analysed population consisted of 398 patients, predominantly male with a median age of 50 years, 58.3 % of whom had comorbidities. Forty-three patients (10.8 %) were diagnosed with cirrhosis. Patients were treated with entecavir, tenofovir, or lamivudine for a median of 5 years. At the start of treatment, the median viral load was 5110 IU/ml, and 98 % of patients achieved viral eradication during the therapy. In the study population, anti-HDV antibodies were detected in 6 patients (1.5 %), 3 men and 3 women, of whom in 1 (0.25 %) the presence of genetic material of the HDV was confirmed by molecular testing.</div><div>Among anti-HDV-positive patients, 3 (50 %) had cirrhosis, 1 of them underwent liver transplantation, and 2 had extrahepatic malignancy. All of them were negative for hepatitis B virus envelope (HBe) antigen and had antibodies to HBe antigen present at the beginning of antiviral treatment. All anti-HDV-positive patients achieved viral clearance during the therapy.</div></div><div><h3>Conclusion</h3><div>In a population of almost four hundred patients infected with HBV, we observed a low prevalence of anti-HDV antibodies of 1.5 % and HDV replication of only 0.25 %. Half of the 6 patients with serologic evidence of co-infection had cirrhosis, so HDV likely played a role in disease progression.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 277-283"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meconium ferritin amounts and birth size of neonates: a pilot study 胎铁蛋白含量与新生儿出生尺寸的初步研究。

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-05-09 DOI: 10.1016/j.advms.2025.05.001

Ewa Skarżyńska , Artur Jakimiuk , Tadeusz Issat , Krzysztof Krasuski , Barbara Lisowska-Myjak

{"title":"Meconium ferritin amounts and birth size of neonates: a pilot study","authors":"Ewa Skarżyńska , Artur Jakimiuk , Tadeusz Issat , Krzysztof Krasuski , Barbara Lisowska-Myjak","doi":"10.1016/j.advms.2025.05.001","DOIUrl":"10.1016/j.advms.2025.05.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Ferritin amounts that accumulate in the meconium may provide new postnatal insights into intrauterine iron homeostasis and neonatal preparedness for the postnatal period. The most dynamic increases in fetal iron stores and fetal growth occur during the third trimester.</div></div><div><h3>Materials and methods</h3><div>This study involved 122 neonates born between 36 and 41 weeks of gestation, with birth weights from 2650 g to 4960 g and birth lengths ranging from 50 cm to 60 cm. Ferritin amounts per gram of meconium were determined via ELISA in the first meconium passed after birth.</div></div><div><h3>Results</h3><div>A significant week-by-week increase in the birth weight and length (<em>p</em> < 0.05) was accompanied by decreasing meconium ferritin amounts (<em>p</em> = 0.021) across the gestational age range of 36–41 weeks. There were negative correlations (p < 0.05) between the systematic decrease in meconium ferritin amounts and the gestational age across the same range (r = -0.18) and between ferritin amounts and the birth weight and length of newborns (r = -0.20 and r = -0.31). Neonates born at 36–37 weeks of gestation had lower birth weight and length, while their meconium ferritin amounts were nearly twice as high as in neonates born at 38–39 weeks or 40–41 weeks (<em>p</em> < 0.05).</div></div><div><h3>Conclusions</h3><div>Systematic decreases in meconium ferritin amounts from 36 to 41 weeks of gestation may suggest a gradual and gestational age-appropriate maturation of the mechanisms responsible for adaptation of the fetus to postnatal life. Determining a cut-off value for meconium ferritin amounts could aid in optimal management of newborns after birth.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 237-242"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations 临床相关浓度的罗哌卡因和利多卡因抑制卵巢癌细胞的增殖和迁移，并引起形态学改变。

IF 2.6 4区医学

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-09-08 DOI: 10.1016/j.advms.2025.08.003

Jane M. Hayden , Andreas Tinnert , Kersti Alm , Jonatan Oras , Linda Block , Anil Gupta , Sven-Egron Thörn , Stina Oredsson

{"title":"Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations","authors":"Jane M. Hayden , Andreas Tinnert , Kersti Alm , Jonatan Oras , Linda Block , Anil Gupta , Sven-Egron Thörn , Stina Oredsson","doi":"10.1016/j.advms.2025.08.003","DOIUrl":"10.1016/j.advms.2025.08.003","url":null,"abstract":"<div><h3>Purpose</h3><div>Ovarian cancer ranks as a gynecological malignancy with poor prognosis, specifically if detected late. Primary treatment includes cytoreductive surgery and adjuvant chemotherapy with curative intent. Local anesthetics (LA) administered in the perioperative period may potentially impact patient outcome by several mechanisms. The beneficial impact of LA has been attributed, among other factors, to the drug's inhibitory effect on cancer cells. The primary aim of the study was to evaluate the effect of clinically relevant concentrations of ropivacaine and lidocaine on ovarian cancer cell lines.</div></div><div><h3>Method</h3><div>Three ovarian cancer cell lines (SKOV-3, SW-626 and CA-OV-3) were treated with 1, 10, 100, or 1000 μM of the two LAs. Cell function and morphology were assessed in the following ways: cell counting, phase-contrast and holographic microscopy, a conventional MTT assay for dose response testing, wound healing assay for migration, and cancer stem cell (CSC) identification by aldehyde dehydrogenase.</div></div><div><h3>Results</h3><div>Both ropivacaine and lidocaine significantly reduced cell number, altered morphology, suppressed migration, and decreased the population of CSCs in a concentration-dependent manner.</div></div><div><h3>Conclusion</h3><div>LAs exert a direct inhibitory effect on ovarian cancer cell lines <em>in vitro</em>, suggesting their potential benefits in perioperative management for patients undergoing surgery. Clinical studies using LA during ovarian cancer surgery are needed.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 326-334"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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