Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration.

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2025-05-24 DOI:10.1016/j.advms.2025.05.002

Jeevitaa Kshersagar, Akshay A Kawale, Leena R Chaudhari, Mrunal N Damle, Rakesh Kumar Sharma, Meghnad G Joshi

{"title":"Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration.","authors":"Jeevitaa Kshersagar, Akshay A Kawale, Leena R Chaudhari, Mrunal N Damle, Rakesh Kumar Sharma, Meghnad G Joshi","doi":"10.1016/j.advms.2025.05.002","DOIUrl":null,"url":null,"abstract":"Purpose: Endometrium, a dynamic tissue undergoing cyclic changes, plays a pivotal role in reproductive health. Disruptions in its structure and function can lead to infertility and pregnancy complications. Stem cell-based therapies, including very small embryonic-like stem cells (VSELS) and platelet-rich plasma (PRP), have shown promise in tissue regeneration.Methods: We investigated the efficacy of bone marrow-derived VSELS combined with thrombin-activated PRP (aPRP) for endometrial regeneration in a murine model of disturbed endometrium (DE). Characterization of bone marrow very small embryonic like stem cells (BM VSELS) revealed pluripotency markers and negative expression for CD34, Tie-2, Thy, CD133, CD90, and delta-like protein (DLK).Results: Transplantation of BM VSELS-aPRP resulted in their engraftment in the endometrium, with enhanced endometrial thickness, collagen reformation, and improved marker expression compared to controls. Immunohistochemical analysis demonstrated increased expression of α-SMA, CK-18, CK-19, E-Cad, Cla-1, CX-40, and ZO-1 in the transplant group. Pregnancy outcomes improved in the BM VSELS-aPRP group, with successful conception and delivery of healthy pups.Conclusion: This study highlights the regenerative potential of BM VSELS-aPRP for endometrial repair and suggests a novel therapeutic approach for endometrial disorders and infertility.","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in medical sciences","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.advms.2025.05.002","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Endometrium, a dynamic tissue undergoing cyclic changes, plays a pivotal role in reproductive health. Disruptions in its structure and function can lead to infertility and pregnancy complications. Stem cell-based therapies, including very small embryonic-like stem cells (VSELS) and platelet-rich plasma (PRP), have shown promise in tissue regeneration.

Methods: We investigated the efficacy of bone marrow-derived VSELS combined with thrombin-activated PRP (aPRP) for endometrial regeneration in a murine model of disturbed endometrium (DE). Characterization of bone marrow very small embryonic like stem cells (BM VSELS) revealed pluripotency markers and negative expression for CD34, Tie-2, Thy, CD133, CD90, and delta-like protein (DLK).

Results: Transplantation of BM VSELS-aPRP resulted in their engraftment in the endometrium, with enhanced endometrial thickness, collagen reformation, and improved marker expression compared to controls. Immunohistochemical analysis demonstrated increased expression of α-SMA, CK-18, CK-19, E-Cad, Cla-1, CX-40, and ZO-1 in the transplant group. Pregnancy outcomes improved in the BM VSELS-aPRP group, with successful conception and delivery of healthy pups.

Conclusion: This study highlights the regenerative potential of BM VSELS-aPRP for endometrial repair and suggests a novel therapeutic approach for endometrial disorders and infertility.

查看原文本刊更多论文

骨髓来源的非常小的胚胎样干细胞与凝血酶激活的富血小板血浆在子宫内膜再生中的治疗潜力。

目的：子宫内膜是一种周期性变化的动态组织，在生殖健康中起着关键作用。其结构和功能的破坏可导致不孕和妊娠并发症。基于干细胞的治疗，包括非常小的胚胎样干细胞（VSELS）和富血小板血浆（PRP），在组织再生方面显示出前景。方法：研究骨髓源性VSELS联合凝血酶激活PRP （aPRP）对小鼠子宫内膜紊乱（DE）模型子宫内膜再生的作用。骨髓非常小胚胎样干细胞（BM VSELS）的表征显示了多能性标记和CD34、Tie-2、Thy、CD133、CD90和δ样蛋白（DLK）的负表达。结果：BM VSELS-aPRP移植后，其在子宫内膜中植入，与对照组相比，子宫内膜厚度增加，胶原蛋白重组，标志物表达改善。免疫组化分析显示移植组α-SMA、CK-18、CK-19、E-Cad、Cla-1、CX-40、ZO-1表达增加。BM VSELS-aPRP组妊娠结局改善，成功受孕并产下健康幼崽。结论：本研究强调了BM VSELS-aPRP在子宫内膜修复中的再生潜力，为子宫内膜疾病和不孕症的治疗提供了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.