Advances in medical sciences最新文献

{"title":"The oxidative stress-associated long non-coding RNAs in pancreatic cancer","authors":"Setayesh Baradaran-Bagherian ,&nbsp;Mahdieh Mehrab Mohseni ,&nbsp;Roya Sharifi ,&nbsp;Roya Amirinejad ,&nbsp;Zeinab Shirvani-Farsani","doi":"10.1016/j.advms.2024.04.006","DOIUrl":"10.1016/j.advms.2024.04.006","url":null,"abstract":"<div><h3>Purpose</h3><p>A lot of people are dying from pancreatic cancer (PC) annually. The early detection of this cancer is particularly challenging due to the fact that symptoms tend to appear in advanced stages. It has been suggested that oxidative stress may play a role in the development of PC. Several genes regulate this process, including long noncoding RNAs (lncRNAs). There is no comprehensive study on the expression pattern of lncRNAs related to oxidative stress in PC patients. In the present case-control study, we quantified levels of oxidative stress-associated lncRNAs in PC patients versus healthy controls.</p></div><div><h3>Patients and methods</h3><p>In the present study, we investigated the expression levels of lincRNA-p21, LUCAT, RMST, FOXD3-AS1, and MT1DP lncRNAs in the peripheral blood mononuclear cells (PBMCs) of 53 ​PC patients and 50 healthy controls. The association between lncRNA expression and clinical and pathological characteristics was also evaluated.</p></div><div><h3>Results</h3><p>The expression of lincRNA-P21 and rhabdomyosarcoma 2-associated transcript (RMST) lncRNAs in PC patients has significantly decreased. Expression of lncRNA RMST was significantly higher in TNM stage III-IV patients in comparison to TNM stage I-II patients. In addition, a significant positive association was recognized between candidate lncRNA expression, and finally, the AUC values of the expression levels of lincRNA-p21 and RMST were 0.60 and 0.61, respectively.</p></div><div><h3>Conclusions</h3><p>Altogether, our study suggests a possible role of lincRNA-p21 and RMST lncRNAs in the etiology of PC pathobiology, and their biomarker role may be understood in future studies.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 231-237"},"PeriodicalIF":2.7,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140778600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidomic approach to identify Escherichia coli and Shigella spp. by matrix-assisted laser desorption/ionization mass spectrometry","authors":"Adrian Arendowski","doi":"10.1016/j.advms.2024.04.005","DOIUrl":"10.1016/j.advms.2024.04.005","url":null,"abstract":"<div><h3>Purpose</h3><p><em>Escherichia coli</em> (<em>E. coli</em>) and <em>Shigella</em> species, being highly similar, present a challenge for differentiation using classical methods such as phenotyping, 16S rRNA sequencing, or protein profiling using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The paper proposes a method for identifying <em>E. coli</em>, <em>S. flexneri</em>, <em>S. sonnei</em>, and <em>S. boydii</em> by augmenting the Bruker Biotyper database with reference spectra of lipid profiles obtained using MALDI MS in the positive mode.</p></div><div><h3>Materials/methods</h3><p>Lipid extracts were made from cultured <em>E. coli, S. flexneri, S. sonnei</em> and <em>S. boydii</em> using the Bligh &amp; Dyer protocol. MALDI MS spectra in positive ion mode were performed for the extracts. Reference spectra were created from 30 spectra for each bacterium and added to the Bruker Biotyper database.</p></div><div><h3>Results</h3><p>Identification of bacteria based on lipid profiles in the Biotyper database gave correct results with scores above 2.49. Statistical analysis of the results by Partial Least Squares-Discriminant Analysis (PLS-DA) showed that it is possible to correctly differentiate the microorganisms studied using the lipidomic approach. A panel of six <em>m/z</em> values was proposed for which the value of the area under the ROC curve is 1, thus enabling the identification of <em>E. coli</em> and <em>S. flexneri</em> with 100 ​% accuracy.</p></div><div><h3>Conclusions</h3><p>Identification of bacteria from lipid fingerprints obtained by the MALDI MS technique is possible and may become a useful tool in the future, especially for microorganisms that are difficult to distinguish by other methods.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 238-247"},"PeriodicalIF":2.7,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140782268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of antazoline versus amiodarone, flecainide and propafenone in restoring sinus rhythm at the emergency department","authors":"Janusz Springer ,&nbsp;Michalina Pejska ,&nbsp;Wojciech Homenda ,&nbsp;Tomasz Zdrojewski ,&nbsp;Ludmiła Daniłowicz-Szymanowicz ,&nbsp;Dariusz Kozłowski","doi":"10.1016/j.advms.2024.04.003","DOIUrl":"10.1016/j.advms.2024.04.003","url":null,"abstract":"<div><h3>Purpose</h3><p>Little is known about the effectiveness of pharmacological cardioversion (PCV) with antazoline in comparison to flecainide. The aim of this study was to compare the effectiveness of antazoline in restoring sinus rhythm (SR) versus amiodarone, flecainide and propafenone in a group of emergency department (ED) patients.</p></div><div><h3>Materials/methods</h3><p>This was a single-centre retrospective analysis of patient records from an ED in a large hospital in Poland. We analysed a total of 1878 patient records, divided based on the anti-arrhythmic drug (AAD) administered during PCV: antazoline (n ​= ​1080), antazoline ​+ ​β-blocker (n ​= ​479), amiodarone (n ​= ​129), flecainide (n ​= ​102), propafenone (n ​= ​88). Of the patients, 63.5 ​% were female (median 65 years, [19–100]).</p></div><div><h3>Results</h3><p>The percentage of successful PCV was significantly higher in the antazoline group (84.3 ​%) than in the antazoline ​+ ​β-blocker (75.8 ​%, p ​= ​0.0001), propafenone (75.6 ​%, p ​= ​0.0364) and amiodarone (68.8 ​%, p ​&lt; ​0.0001) groups. Post-hoc analysis revealed that patients who received PCV with antazoline, antazoline ​+ ​β-blocker, flecainide and propafenone had significantly shorter time to SR than those who received amiodarone (p ​&lt; ​0.0001). Univariate regression analysis revealed that patients who underwent PCV with antazoline were almost twice as likely to return to SR compared to the other groups (p ​&lt; ​0.0001, OR 1.81, 95 ​% CI 1.44–2.27).</p></div><div><h3>Conclusions</h3><p>This is the first study comparing the effectiveness of antazoline in PCV versus flecainide in addition to the previously studied amiodarone and propafenone. Our results indicate that antazoline is more effective in restoring SR than amiodarone, flecainide and propafenone. In addition, antazoline restored SR significantly faster than amiodarone or propafenone.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 248-255"},"PeriodicalIF":2.7,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140793335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis","authors":"Julia Załęcka ,&nbsp;Zuzanna Zielińska ,&nbsp;Łukasz Ołdak ,&nbsp;Agata Sakowicz ,&nbsp;Grzegorz Mańka ,&nbsp;Mariusz Kiecka ,&nbsp;Robert Spaczyński ,&nbsp;Piotr Piekarski ,&nbsp;Beata Banaszewska ,&nbsp;Artur Jakimiuk ,&nbsp;Tadeusz Issat ,&nbsp;Jakub Młodawski ,&nbsp;Maria Szubert ,&nbsp;Piotr Sieroszewski ,&nbsp;Grzegorz Raba ,&nbsp;Kamil Szczupak ,&nbsp;Tomasz Kluz ,&nbsp;Marek Kluza ,&nbsp;Piotr Pierzyński ,&nbsp;Michał Ciebiera ,&nbsp;Piotr Laudański","doi":"10.1016/j.advms.2024.04.004","DOIUrl":"https://doi.org/10.1016/j.advms.2024.04.004","url":null,"abstract":"<div><h3>Purpose</h3><p>Endometriosis is a common disease with a complex pathomechanism and atypical symptoms, often leading to delayed diagnosis. Currently, the sole method for confirming the presence of the disease is through laparoscopy and histopathological examination of collected tissue. However, this invasive procedure carries potential risk and complications, necessitating the exploration of non-surgical diagnostic methods for endometriosis. This study aims to analyze peritoneal fluid and plasma samples for the expression of cathepsin L and cathepsin S to identify potential biomarkers for non-invasive diagnostic approaches to endometriosis.</p></div><div><h3>Material and methods</h3><p>In this cross-sectional study, plasma and peritoneal fluid samples were obtained during laparoscopy from 63 patients diagnosed with chronic pelvic pain or infertility. The study group consisted of women with confirmed endometriosis. The concentrations of cathepsins L and S were determined using an SPRi biosensor.</p></div><div><h3>Results</h3><p>The study did not reveal significant differences in the concentrations of cathepsin L and cathepsin S between the control group and the study group, both in peritoneal fluid and plasma.</p></div><div><h3>Conclusions</h3><p>Based on the results of this study, it appears that cathepsins L and S are not suitable candidates as biomarkers for endometriosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 224-230"},"PeriodicalIF":2.7,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell sequencing analysis confirms the association of ANRIL with the increased smooth muscle cell proliferation and migration gene signatures in pulmonary artery hypertension in silico","authors":"Yan Li,&nbsp;Yan-Tong Zhang,&nbsp;Bing Han,&nbsp;Lan Xue,&nbsp;Yan Wei,&nbsp;Ge Li","doi":"10.1016/j.advms.2024.04.002","DOIUrl":"https://doi.org/10.1016/j.advms.2024.04.002","url":null,"abstract":"<div><h3>Purpose</h3><p>Smooth muscle cell (SMC) dysregulation is part of the pathological basis of pulmonary artery hypertension (PAH). We aimed to explore the heterogeneity of SMCs in PAH.</p></div><div><h3>Methods</h3><p>The profile GSE210248 was obtained from NCBI Gene Expression Omnibus, containing the scRNA-seq data of pulmonary arteries (PA) from three patients with PAH and three healthy donors. After quality control, normalization, and dimension reduction, cell clustering analysis was performed. Differential expression analysis and functional enrichment analysis were carried out successively in smooth muscle cells (SMCs). The enrichment scores of cell cycle and cell migration gene sets in SMCs were calculated. Then, the Spearman correlation coefficients between antisense non-coding RNA in the INK4 locus (ANRIL) expression and two gene sets were computed.</p></div><div><h3>Results</h3><p>Eight cell clusters were identified in PA from samples. The proportion of SMCs was increased in PAH samples. SMCs were divided into five subclusters with diverse biological functions. Muscle contraction-related SMC1 was decreased, while extracellular matrix organization-related SMC2, immune and inflammatory response-related SMC4 and SMC5 were increased in PAH samples compared with healthy donors. The enrichment scores of cell cycle and cell migration gene sets in SMCs were higher in PAH samples than in donors. ANRIL was down-regulated significantly in PAH samples and was negatively related to the scores of two gene sets.</p></div><div><h3>Conclusion</h3><p>SMCs exhibited significant heterogeneity in PAH. The altered abilities of SMC proliferation and migration in PAH were associated with ANRIL expression.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 217-223"},"PeriodicalIF":2.7,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140640958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrobium officinale Polysaccharide (DOP) inhibits cell hyperproliferation, inflammation and oxidative stress to improve keratinocyte psoriasis-like state","authors":"Bijun Zeng ,&nbsp;Yining Yan ,&nbsp;Yujin Zhang ,&nbsp;Chang Wang ,&nbsp;Wenting Huang ,&nbsp;Xinyi Zhong ,&nbsp;Zi Chen ,&nbsp;Mengzhou Xie ,&nbsp;Zhibo Yang","doi":"10.1016/j.advms.2024.03.005","DOIUrl":"10.1016/j.advms.2024.03.005","url":null,"abstract":"<div><h3>Purpose</h3><p>Psoriasis is a skin disease characterized by excessive proliferation, inflammation and oxidative stress in keratinocytes. The present study aimed to investigate the therapeutic effects of <em>Dendrobium officinale</em> polysaccharide (DOP) on keratinocyte psoriasis-like models.</p></div><div><h3>Methods</h3><p>The HaCaT keratinocyte inflammation models were induced by interleukin (IL)-22 or lipopolysaccharide (LPS), respectively, and oxidative stress damage within cells was elicited by H₂O₂ and treated using DOP. CCK-8 and EdU were carried out to detect cell proliferation. ELISA, qRT-PCR, and Western blot were conducted to measure the expression of pro-inflammatory cytokines IL17A, IL-23, IL1β, tumor necrosis factor alpha (TNF-α), and IL-6. Reactive oxygen species (ROS) level in keratinocytes was detected by flow cytometry. Cell proliferation-associated proteins (PCNA, Ki67, Cyclin D1) and pathway proteins (p-AKT and AKT), and oxidative stress marker proteins (Nrf-2, CAT, SOD1) were detected by Western blot.</p></div><div><h3>Result</h3><p>DOP did not affect the proliferation of normal keratinocytes, but DOP was able to inhibit the proliferative activity of IL-22-induced overproliferating keratinocytes and suppress the expression of proliferation-related factors PCNA, Ki67, and Cyclin D1 as well as the proliferation pathway p-AKT. In addition, DOP treatment was able to inhibit IL-22 and LPS-induced inflammation and H₂O₂-induced oxidative stress, including the expression of IL17A, IL-23, IL1β, TNF-α, IL-6, and IL1β, as well as the expression levels of intracellular ROS levels and cellular oxidative stress-related indicators SOD, MDA, CAT, Nrf-2 and SOD1.</p></div><div><h3>Conclusion</h3><p>DOP inhibits keratinocyte hyperproliferation, inflammation and oxidative stress to improve the keratinocyte psoriasis-like state.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 167-175"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of wound-healing assay, phorbol myristate acetate (PMA) stimulation and siRNA-mediated FURIN gene silencing on endogenous retroviral ERVW-1 expression level in U87-MG astrocytoma cells","authors":"Grzegorz Machnik ,&nbsp;Łukasz Bułdak ,&nbsp;Karolina Zapletal-Pudełko ,&nbsp;Beniamin Oskar Grabarek ,&nbsp;Rafał Staszkiewicz ,&nbsp;Dawid Sobański ,&nbsp;Bogusław Okopień","doi":"10.1016/j.advms.2024.02.007","DOIUrl":"10.1016/j.advms.2024.02.007","url":null,"abstract":"<div><h3>Purpose</h3><p>Human endogenous retroviruses (HERVs) are ubiquitous genomic sequences. Normally dormant HERVs, undergo reactivation by environmental factors. This deregulation of HERVs’ transcriptional equilibrium correlates with medical conditions such as multiple sclerosis (MS). Here we sought to explore whether exposing the U-87 MG astrocytoma cells to traumatic injury deregulates the expression of HERV-W family member <em>ERVW-1</em> encoding syncytin-1. We also examined the expression of <em>FURIN</em> gene that is crucial in syncytin-1 synthesis.</p></div><div><h3>Material and methods</h3><p>Scratch assay was used as a model of cells injury in U-87 MG cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot (WB) and migration assay using Boyden chamber were used. Phorbol 12-myristate 13-acetate (PMA) and small interfering RNA (siRNA) were used for cell stimulation and gene expression inhibition, respectively.</p></div><div><h3>Results</h3><p>Results revealed reduced <em>ERVW-1</em> expression in cells exposed to injury (p ​&lt; ​0.05) while <em>GFAP</em> gene - a marker of active astrocytes, was upregulated (p ​&lt; ​0.01). These findings were confirmed by both WB and RT-qPCR. Expression of <em>FURIN</em> gene was not altered after injury, but cell stimulation by PMA strongly increased <em>FURIN</em> expression, simultaneously downregulating <em>ERVW-1</em> (p ​&lt; ​0.01). SiRNA-mediated expression inhibition of <em>ERVW-1</em> and <em>FURIN</em> influenced the mRNA level for <em>SLC1A5</em> (<em>ASCT2</em>) - primary syncytin-1 receptor, that was significantly lower. <em>FURIN</em> inhibition by siRNA caused strong upregulation of <em>ERVW-1</em> expression (p ​&lt; ​0.01).</p></div><div><h3>Conclusion</h3><p>Results showed that mechanical impact affects the expression of endogenous retroviruses in U-87 MG astrocytoma cells by scratch assay. Regulation of <em>FURIN</em>, a crucial enzyme in <em>ERVW-1</em> turnover may support the therapy of some neurological conditions.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 113-124"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphopenia associated with survivin and its downstream pathway in COVID-19 serving as a potential route in COVID-19 pathogenesis","authors":"Mohammad Saeed Kahrizi ,&nbsp;Kamyar Nasiri ,&nbsp;Farnoosh Ebrahimzadeh ,&nbsp;Amirhossein Fakhre Yaseri ,&nbsp;Soroush Ghodratizadeh ,&nbsp;Mostafa Gholamrezaei ,&nbsp;Alireza Rahat Dahmardeh ,&nbsp;Ali Adili ,&nbsp;Rosita Amjidifar ,&nbsp;Maryam Hemmatzadeh ,&nbsp;Mohsen Arabi ,&nbsp;Mohammad Reza Maghsoudi ,&nbsp;Hamed Mohammadi","doi":"10.1016/j.advms.2024.03.006","DOIUrl":"10.1016/j.advms.2024.03.006","url":null,"abstract":"<div><h3>Purpose</h3><p>Starting in 2019, coronavirus disease 2019 (COVID-19) caused an epidemic that was growing rapidly and has harmed millions of people globally. It has been demonstrated that survivin regulates lymphocyte survival, a main route involved in COVID-19 pathogenesis. Survivin belongs to the inhibitor of apoptosis protein (IAP) family, and its primary functions comprise regulating mitosis and inhibiting apoptosis. Since lower survivin expression has been shown to increase the sensitivity of lymphocytes to apoptotic induction, we looked into the function of survivin and its corresponding pathways in COVID-19 pathogenesis.</p></div><div><h3>Materials and methods</h3><p>The expression of survivin, X-linked inhibitor of apoptosis protein (XIAP), caspases 3, 7, 9, and poly (ADP-ribose) polymerase (PARP) was evaluated at both mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) derived from healthy donors and patients with severe and moderate COVID-19 by qRT-PCR and Western blotting, respectively. Then, we enforced apoptosis to COVID-19 patient-derived lymphocytes, and the percent was assessed by flow cytometry.</p></div><div><h3>Results</h3><p>Survivin and XIAP were less expressed in PBMCs derived from COVID-19 patients as apoptosis inhibitors than PARP, cleaved-PARP, caspase 9, and cleaved caspases 3 and 7, according to the results of real-time PCR and Western blot analysis. Additionally, according to the flow cytometry results, the down-regulation of survivin served as a potential factor in the lymphocyte depletion observed in patients with COVID-19.</p></div><div><h3>Conclusion</h3><p>The role of survivin and its related pathway was first discovered in the development of COVID-19 and may serve as a potential prognostic and therapeutic target.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 190-197"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intensity-modulated radiotherapy combined with chemotherapy and prognostic factors in patients with locally advanced oesophageal cancer","authors":"Yao Ou ,&nbsp;Hongbo Li ,&nbsp;Dan Yang ,&nbsp;Lijun Suo","doi":"10.1016/j.advms.2024.03.001","DOIUrl":"10.1016/j.advms.2024.03.001","url":null,"abstract":"<div><h3>Purpose</h3><p>We explored the efficacy and influencing factors of chemoradiotherapy and radiotherapy alone in patients with locally advanced oesophageal squamous cell carcinoma.</p></div><div><h3>Methods</h3><p>We retrospectively analysed 226 locally advanced oesophageal squamous cell carcinoma patients who underwent chemoradiotherapy and radiotherapy alone. Univariate and multivariate Cox regression analyses were used to analyse the impact of relevant factors. The endpoint was overall survival and progression-free survival.</p></div><div><h3>Results</h3><p>Compared with the radiotherapy group, the chemoradiotherapy group had a significant difference in the overall survival rate and the progression-free survival rate between 3 and 5 years (both p ​&lt; ​0.05). The incidences of radiation pneumonitis and radiation oesophagitis were analysed, and the differences were not significant (all p ​&gt; ​0.05). The incidence of haematological toxicity in the chemoradiotherapy group was significantly higher than that in the radiotherapy group (p ​= ​0.001). There was a significant difference in the incidence of haematological toxicity between the ≤65 and the &gt;65 age groups (p ​&lt; ​0.05). Tumour location, T stage, tumour length, tumour target volume, and short-term curative effect were the main factors affecting the prognosis (all p ​&lt; ​0.05). T stage, gross tumour volume, and short-term curative effect were all independent factors affecting the prognosis (all p ​&lt; ​0.05).</p></div><div><h3>Conclusions</h3><p>Patients with locally advanced oesophageal cancer who received intensity-modulated radiotherapy (IMRT) combined with chemotherapy had significant survival benefits compared with radiotherapy alone. Haematological toxicity was the main adverse reaction. T-stage, gross tumour volume and short-term curative effect were independent factors influencing the prognosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 125-131"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000142/pdfft?md5=b0f040120276190f172b80b975ec6c64&pid=1-s2.0-S1896112624000142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and long-term outcomes of patients with heart failure with improved ejection fraction. First Polish experience from LECRA-HF registry","authors":"Konrad Stępień ,&nbsp;Karol Nowak ,&nbsp;Natalia Kachnic ,&nbsp;Aleksandra Karcińska ,&nbsp;Alicia del Carmen Yika ,&nbsp;Jakub Furczyński ,&nbsp;Michael Platschek ,&nbsp;Maria Skorupa ,&nbsp;Zuzanna Wyleciał ,&nbsp;Jarosław Zalewski ,&nbsp;Jadwiga Nessler","doi":"10.1016/j.advms.2024.02.009","DOIUrl":"10.1016/j.advms.2024.02.009","url":null,"abstract":"<div><h3>Purpose</h3><p>Heart failure (HF) with improved ejection fraction (HFimpEF) is a new category of HF introduced in the newest European Society of Cardiology guidelines. However, clinical characteristics and long-term outcomes of HFimpEF patients remain insufficiently elucidated. We sought to characterize Polish HFimpEF patients and determine their long-term mortality.</p></div><div><h3>Material and methods</h3><p>Of 1186 patients enrolled in the single-center Lesser Poland Cracovian Heart Failure (LECRA-HF) registry between 2009 and 2019 and hospitalized due to HF decompensation, 340 (28.7%) were those with HF with reduced ejection fraction (HFrEF). Based on follow-up echocardiography, 61 (17.9%) of them were classified as HFimpEF and the remaining as HFnon-impEF.</p></div><div><h3>Results</h3><p>HFimpEF patients were more frequently females (P ​&lt; ​0.001), had higher baseline left ventricular ejection fraction (LVEF, P ​&lt; ​0.001), had less often a history of diabetes (P ​= ​0.024), severe chronic kidney disease (P ​= ​0.026) or prior myocardial infarction (P ​= ​0.008) than HFnon-impEF patients. By multivariable analysis the HFimpEF diagnosis was independently predicted by baseline NYHA I/II (odds ratio [OR] 2.347, 95% confidence interval [95%CI] 1.020–5.405), non-ischemic etiology (OR 3.096, 95%CI 1.587–6.024), lack of diabetes mellitus (OR 2.016, 95%CI 1.059–3.846) and higher baseline LVEF (OR 1.084, 95%CI 1.042–1.126, per 1%). Within the median 49 (25–77) months all-cause mortality was lower in HFimpEF than in HFnon-impEF (10.8 vs 16.4%/year, P ​= ​0.004).</p></div><div><h3>Conclusions</h3><p>Our findings indicate that every sixth Polish patient with HFrEF has a chance to improve LVEF during follow-up and to become a HFimpEF patient. Baseline characteristics of HFimpEF patients are different from HFnon-impEF. Simultaneously, the HFimpEF diagnosis is associated with higher long-term survival.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 132-138"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}