Advances in medical sciences最新文献

{"title":"The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis","authors":"Julia Załęcka ,&nbsp;Zuzanna Zielińska ,&nbsp;Łukasz Ołdak ,&nbsp;Agata Sakowicz ,&nbsp;Grzegorz Mańka ,&nbsp;Mariusz Kiecka ,&nbsp;Robert Spaczyński ,&nbsp;Piotr Piekarski ,&nbsp;Beata Banaszewska ,&nbsp;Artur Jakimiuk ,&nbsp;Tadeusz Issat ,&nbsp;Jakub Młodawski ,&nbsp;Maria Szubert ,&nbsp;Piotr Sieroszewski ,&nbsp;Grzegorz Raba ,&nbsp;Kamil Szczupak ,&nbsp;Tomasz Kluz ,&nbsp;Marek Kluza ,&nbsp;Piotr Pierzyński ,&nbsp;Michał Ciebiera ,&nbsp;Piotr Laudański","doi":"10.1016/j.advms.2024.04.004","DOIUrl":"https://doi.org/10.1016/j.advms.2024.04.004","url":null,"abstract":"<div><h3>Purpose</h3><p>Endometriosis is a common disease with a complex pathomechanism and atypical symptoms, often leading to delayed diagnosis. Currently, the sole method for confirming the presence of the disease is through laparoscopy and histopathological examination of collected tissue. However, this invasive procedure carries potential risk and complications, necessitating the exploration of non-surgical diagnostic methods for endometriosis. This study aims to analyze peritoneal fluid and plasma samples for the expression of cathepsin L and cathepsin S to identify potential biomarkers for non-invasive diagnostic approaches to endometriosis.</p></div><div><h3>Material and methods</h3><p>In this cross-sectional study, plasma and peritoneal fluid samples were obtained during laparoscopy from 63 patients diagnosed with chronic pelvic pain or infertility. The study group consisted of women with confirmed endometriosis. The concentrations of cathepsins L and S were determined using an SPRi biosensor.</p></div><div><h3>Results</h3><p>The study did not reveal significant differences in the concentrations of cathepsin L and cathepsin S between the control group and the study group, both in peritoneal fluid and plasma.</p></div><div><h3>Conclusions</h3><p>Based on the results of this study, it appears that cathepsins L and S are not suitable candidates as biomarkers for endometriosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 224-230"},"PeriodicalIF":2.7,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell sequencing analysis confirms the association of ANRIL with the increased smooth muscle cell proliferation and migration gene signatures in pulmonary artery hypertension in silico","authors":"Yan Li,&nbsp;Yan-Tong Zhang,&nbsp;Bing Han,&nbsp;Lan Xue,&nbsp;Yan Wei,&nbsp;Ge Li","doi":"10.1016/j.advms.2024.04.002","DOIUrl":"https://doi.org/10.1016/j.advms.2024.04.002","url":null,"abstract":"<div><h3>Purpose</h3><p>Smooth muscle cell (SMC) dysregulation is part of the pathological basis of pulmonary artery hypertension (PAH). We aimed to explore the heterogeneity of SMCs in PAH.</p></div><div><h3>Methods</h3><p>The profile GSE210248 was obtained from NCBI Gene Expression Omnibus, containing the scRNA-seq data of pulmonary arteries (PA) from three patients with PAH and three healthy donors. After quality control, normalization, and dimension reduction, cell clustering analysis was performed. Differential expression analysis and functional enrichment analysis were carried out successively in smooth muscle cells (SMCs). The enrichment scores of cell cycle and cell migration gene sets in SMCs were calculated. Then, the Spearman correlation coefficients between antisense non-coding RNA in the INK4 locus (ANRIL) expression and two gene sets were computed.</p></div><div><h3>Results</h3><p>Eight cell clusters were identified in PA from samples. The proportion of SMCs was increased in PAH samples. SMCs were divided into five subclusters with diverse biological functions. Muscle contraction-related SMC1 was decreased, while extracellular matrix organization-related SMC2, immune and inflammatory response-related SMC4 and SMC5 were increased in PAH samples compared with healthy donors. The enrichment scores of cell cycle and cell migration gene sets in SMCs were higher in PAH samples than in donors. ANRIL was down-regulated significantly in PAH samples and was negatively related to the scores of two gene sets.</p></div><div><h3>Conclusion</h3><p>SMCs exhibited significant heterogeneity in PAH. The altered abilities of SMC proliferation and migration in PAH were associated with ANRIL expression.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 217-223"},"PeriodicalIF":2.7,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140640958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrobium officinale Polysaccharide (DOP) inhibits cell hyperproliferation, inflammation and oxidative stress to improve keratinocyte psoriasis-like state","authors":"Bijun Zeng ,&nbsp;Yining Yan ,&nbsp;Yujin Zhang ,&nbsp;Chang Wang ,&nbsp;Wenting Huang ,&nbsp;Xinyi Zhong ,&nbsp;Zi Chen ,&nbsp;Mengzhou Xie ,&nbsp;Zhibo Yang","doi":"10.1016/j.advms.2024.03.005","DOIUrl":"10.1016/j.advms.2024.03.005","url":null,"abstract":"<div><h3>Purpose</h3><p>Psoriasis is a skin disease characterized by excessive proliferation, inflammation and oxidative stress in keratinocytes. The present study aimed to investigate the therapeutic effects of <em>Dendrobium officinale</em> polysaccharide (DOP) on keratinocyte psoriasis-like models.</p></div><div><h3>Methods</h3><p>The HaCaT keratinocyte inflammation models were induced by interleukin (IL)-22 or lipopolysaccharide (LPS), respectively, and oxidative stress damage within cells was elicited by H₂O₂ and treated using DOP. CCK-8 and EdU were carried out to detect cell proliferation. ELISA, qRT-PCR, and Western blot were conducted to measure the expression of pro-inflammatory cytokines IL17A, IL-23, IL1β, tumor necrosis factor alpha (TNF-α), and IL-6. Reactive oxygen species (ROS) level in keratinocytes was detected by flow cytometry. Cell proliferation-associated proteins (PCNA, Ki67, Cyclin D1) and pathway proteins (p-AKT and AKT), and oxidative stress marker proteins (Nrf-2, CAT, SOD1) were detected by Western blot.</p></div><div><h3>Result</h3><p>DOP did not affect the proliferation of normal keratinocytes, but DOP was able to inhibit the proliferative activity of IL-22-induced overproliferating keratinocytes and suppress the expression of proliferation-related factors PCNA, Ki67, and Cyclin D1 as well as the proliferation pathway p-AKT. In addition, DOP treatment was able to inhibit IL-22 and LPS-induced inflammation and H₂O₂-induced oxidative stress, including the expression of IL17A, IL-23, IL1β, TNF-α, IL-6, and IL1β, as well as the expression levels of intracellular ROS levels and cellular oxidative stress-related indicators SOD, MDA, CAT, Nrf-2 and SOD1.</p></div><div><h3>Conclusion</h3><p>DOP inhibits keratinocyte hyperproliferation, inflammation and oxidative stress to improve the keratinocyte psoriasis-like state.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 167-175"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of wound-healing assay, phorbol myristate acetate (PMA) stimulation and siRNA-mediated FURIN gene silencing on endogenous retroviral ERVW-1 expression level in U87-MG astrocytoma cells","authors":"Grzegorz Machnik ,&nbsp;Łukasz Bułdak ,&nbsp;Karolina Zapletal-Pudełko ,&nbsp;Beniamin Oskar Grabarek ,&nbsp;Rafał Staszkiewicz ,&nbsp;Dawid Sobański ,&nbsp;Bogusław Okopień","doi":"10.1016/j.advms.2024.02.007","DOIUrl":"10.1016/j.advms.2024.02.007","url":null,"abstract":"<div><h3>Purpose</h3><p>Human endogenous retroviruses (HERVs) are ubiquitous genomic sequences. Normally dormant HERVs, undergo reactivation by environmental factors. This deregulation of HERVs’ transcriptional equilibrium correlates with medical conditions such as multiple sclerosis (MS). Here we sought to explore whether exposing the U-87 MG astrocytoma cells to traumatic injury deregulates the expression of HERV-W family member <em>ERVW-1</em> encoding syncytin-1. We also examined the expression of <em>FURIN</em> gene that is crucial in syncytin-1 synthesis.</p></div><div><h3>Material and methods</h3><p>Scratch assay was used as a model of cells injury in U-87 MG cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot (WB) and migration assay using Boyden chamber were used. Phorbol 12-myristate 13-acetate (PMA) and small interfering RNA (siRNA) were used for cell stimulation and gene expression inhibition, respectively.</p></div><div><h3>Results</h3><p>Results revealed reduced <em>ERVW-1</em> expression in cells exposed to injury (p ​&lt; ​0.05) while <em>GFAP</em> gene - a marker of active astrocytes, was upregulated (p ​&lt; ​0.01). These findings were confirmed by both WB and RT-qPCR. Expression of <em>FURIN</em> gene was not altered after injury, but cell stimulation by PMA strongly increased <em>FURIN</em> expression, simultaneously downregulating <em>ERVW-1</em> (p ​&lt; ​0.01). SiRNA-mediated expression inhibition of <em>ERVW-1</em> and <em>FURIN</em> influenced the mRNA level for <em>SLC1A5</em> (<em>ASCT2</em>) - primary syncytin-1 receptor, that was significantly lower. <em>FURIN</em> inhibition by siRNA caused strong upregulation of <em>ERVW-1</em> expression (p ​&lt; ​0.01).</p></div><div><h3>Conclusion</h3><p>Results showed that mechanical impact affects the expression of endogenous retroviruses in U-87 MG astrocytoma cells by scratch assay. Regulation of <em>FURIN</em>, a crucial enzyme in <em>ERVW-1</em> turnover may support the therapy of some neurological conditions.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 113-124"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphopenia associated with survivin and its downstream pathway in COVID-19 serving as a potential route in COVID-19 pathogenesis","authors":"Mohammad Saeed Kahrizi ,&nbsp;Kamyar Nasiri ,&nbsp;Farnoosh Ebrahimzadeh ,&nbsp;Amirhossein Fakhre Yaseri ,&nbsp;Soroush Ghodratizadeh ,&nbsp;Mostafa Gholamrezaei ,&nbsp;Alireza Rahat Dahmardeh ,&nbsp;Ali Adili ,&nbsp;Rosita Amjidifar ,&nbsp;Maryam Hemmatzadeh ,&nbsp;Mohsen Arabi ,&nbsp;Mohammad Reza Maghsoudi ,&nbsp;Hamed Mohammadi","doi":"10.1016/j.advms.2024.03.006","DOIUrl":"10.1016/j.advms.2024.03.006","url":null,"abstract":"<div><h3>Purpose</h3><p>Starting in 2019, coronavirus disease 2019 (COVID-19) caused an epidemic that was growing rapidly and has harmed millions of people globally. It has been demonstrated that survivin regulates lymphocyte survival, a main route involved in COVID-19 pathogenesis. Survivin belongs to the inhibitor of apoptosis protein (IAP) family, and its primary functions comprise regulating mitosis and inhibiting apoptosis. Since lower survivin expression has been shown to increase the sensitivity of lymphocytes to apoptotic induction, we looked into the function of survivin and its corresponding pathways in COVID-19 pathogenesis.</p></div><div><h3>Materials and methods</h3><p>The expression of survivin, X-linked inhibitor of apoptosis protein (XIAP), caspases 3, 7, 9, and poly (ADP-ribose) polymerase (PARP) was evaluated at both mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) derived from healthy donors and patients with severe and moderate COVID-19 by qRT-PCR and Western blotting, respectively. Then, we enforced apoptosis to COVID-19 patient-derived lymphocytes, and the percent was assessed by flow cytometry.</p></div><div><h3>Results</h3><p>Survivin and XIAP were less expressed in PBMCs derived from COVID-19 patients as apoptosis inhibitors than PARP, cleaved-PARP, caspase 9, and cleaved caspases 3 and 7, according to the results of real-time PCR and Western blot analysis. Additionally, according to the flow cytometry results, the down-regulation of survivin served as a potential factor in the lymphocyte depletion observed in patients with COVID-19.</p></div><div><h3>Conclusion</h3><p>The role of survivin and its related pathway was first discovered in the development of COVID-19 and may serve as a potential prognostic and therapeutic target.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 190-197"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intensity-modulated radiotherapy combined with chemotherapy and prognostic factors in patients with locally advanced oesophageal cancer","authors":"Yao Ou ,&nbsp;Hongbo Li ,&nbsp;Dan Yang ,&nbsp;Lijun Suo","doi":"10.1016/j.advms.2024.03.001","DOIUrl":"10.1016/j.advms.2024.03.001","url":null,"abstract":"<div><h3>Purpose</h3><p>We explored the efficacy and influencing factors of chemoradiotherapy and radiotherapy alone in patients with locally advanced oesophageal squamous cell carcinoma.</p></div><div><h3>Methods</h3><p>We retrospectively analysed 226 locally advanced oesophageal squamous cell carcinoma patients who underwent chemoradiotherapy and radiotherapy alone. Univariate and multivariate Cox regression analyses were used to analyse the impact of relevant factors. The endpoint was overall survival and progression-free survival.</p></div><div><h3>Results</h3><p>Compared with the radiotherapy group, the chemoradiotherapy group had a significant difference in the overall survival rate and the progression-free survival rate between 3 and 5 years (both p ​&lt; ​0.05). The incidences of radiation pneumonitis and radiation oesophagitis were analysed, and the differences were not significant (all p ​&gt; ​0.05). The incidence of haematological toxicity in the chemoradiotherapy group was significantly higher than that in the radiotherapy group (p ​= ​0.001). There was a significant difference in the incidence of haematological toxicity between the ≤65 and the &gt;65 age groups (p ​&lt; ​0.05). Tumour location, T stage, tumour length, tumour target volume, and short-term curative effect were the main factors affecting the prognosis (all p ​&lt; ​0.05). T stage, gross tumour volume, and short-term curative effect were all independent factors affecting the prognosis (all p ​&lt; ​0.05).</p></div><div><h3>Conclusions</h3><p>Patients with locally advanced oesophageal cancer who received intensity-modulated radiotherapy (IMRT) combined with chemotherapy had significant survival benefits compared with radiotherapy alone. Haematological toxicity was the main adverse reaction. T-stage, gross tumour volume and short-term curative effect were independent factors influencing the prognosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 125-131"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000142/pdfft?md5=b0f040120276190f172b80b975ec6c64&pid=1-s2.0-S1896112624000142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and long-term outcomes of patients with heart failure with improved ejection fraction. First Polish experience from LECRA-HF registry","authors":"Konrad Stępień ,&nbsp;Karol Nowak ,&nbsp;Natalia Kachnic ,&nbsp;Aleksandra Karcińska ,&nbsp;Alicia del Carmen Yika ,&nbsp;Jakub Furczyński ,&nbsp;Michael Platschek ,&nbsp;Maria Skorupa ,&nbsp;Zuzanna Wyleciał ,&nbsp;Jarosław Zalewski ,&nbsp;Jadwiga Nessler","doi":"10.1016/j.advms.2024.02.009","DOIUrl":"10.1016/j.advms.2024.02.009","url":null,"abstract":"<div><h3>Purpose</h3><p>Heart failure (HF) with improved ejection fraction (HFimpEF) is a new category of HF introduced in the newest European Society of Cardiology guidelines. However, clinical characteristics and long-term outcomes of HFimpEF patients remain insufficiently elucidated. We sought to characterize Polish HFimpEF patients and determine their long-term mortality.</p></div><div><h3>Material and methods</h3><p>Of 1186 patients enrolled in the single-center Lesser Poland Cracovian Heart Failure (LECRA-HF) registry between 2009 and 2019 and hospitalized due to HF decompensation, 340 (28.7%) were those with HF with reduced ejection fraction (HFrEF). Based on follow-up echocardiography, 61 (17.9%) of them were classified as HFimpEF and the remaining as HFnon-impEF.</p></div><div><h3>Results</h3><p>HFimpEF patients were more frequently females (P ​&lt; ​0.001), had higher baseline left ventricular ejection fraction (LVEF, P ​&lt; ​0.001), had less often a history of diabetes (P ​= ​0.024), severe chronic kidney disease (P ​= ​0.026) or prior myocardial infarction (P ​= ​0.008) than HFnon-impEF patients. By multivariable analysis the HFimpEF diagnosis was independently predicted by baseline NYHA I/II (odds ratio [OR] 2.347, 95% confidence interval [95%CI] 1.020–5.405), non-ischemic etiology (OR 3.096, 95%CI 1.587–6.024), lack of diabetes mellitus (OR 2.016, 95%CI 1.059–3.846) and higher baseline LVEF (OR 1.084, 95%CI 1.042–1.126, per 1%). Within the median 49 (25–77) months all-cause mortality was lower in HFimpEF than in HFnon-impEF (10.8 vs 16.4%/year, P ​= ​0.004).</p></div><div><h3>Conclusions</h3><p>Our findings indicate that every sixth Polish patient with HFrEF has a chance to improve LVEF during follow-up and to become a HFimpEF patient. Baseline characteristics of HFimpEF patients are different from HFnon-impEF. Simultaneously, the HFimpEF diagnosis is associated with higher long-term survival.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 132-138"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New-onset diabetes after kidney transplantation: Assessing urinary Wilm's tumor-1 protein to predict renal allograft dysfunction","authors":"César Endrigo Silva de Andrade ,&nbsp;Karla Simone Costa de Souza ,&nbsp;Ony Araújo Galdino ,&nbsp;Mabelle Alves Ferreira de Lima ,&nbsp;Paulo José de Medeiros ,&nbsp;Marcela Abbott Galvão Ururahy ,&nbsp;Maurício Galvão Pereira ,&nbsp;José Bruno de Almeida ,&nbsp;Adriana Augusto de Rezende","doi":"10.1016/j.advms.2024.03.002","DOIUrl":"10.1016/j.advms.2024.03.002","url":null,"abstract":"<div><h3>Purpose</h3><p>New-onset diabetes after transplantation (NODAT) is a frequent metabolic complication associated with podocyte damage and renal allograft dysfunction. Thus, Wilm's tumor-1 (WT-1) protein, as a podocyte marker, holds promise as an option to evaluate renal allograft dysfunction in NODAT. Therefore, the study aimed to investigate urinary WT-1 levels in NODAT patients during the first year after kidney transplantation (KTx).</p></div><div><h3>Materials and methods</h3><p>KTx patients were categorized into non-NODAT and NODAT groups. Fasting blood glucose, glycated hemoglobin (HbA1c), urinary albumin/creatinine ratio (ACR), serum creatinine, estimated glomerular filtration rate (eGFR), and urinary WT-1 were measured at 3, 6, 9, and 12-months post-KTx.</p></div><div><h3>Results</h3><p>The NODAT group manifested elevated levels of blood glucose and HbA1c during the first year post-KTx. Also, exhibited elevations in ACR and serum creatinine levels at 6, 9, and 12-months post-KTx when compared to non-NODAT group. Conversely, eGFR values in the NODAT group demonstrated significant declines at 3, 6, and 9-months post-KTx relative to non-NODAT. Furthermore, NODAT group exhibited a median annual eGFR of 47 ​mL/min/1.73 ​m². Urinary WT-1 levels at 3, 6, 9, and 12-months post-KTx were significantly higher in the NODAT group compared to non-NODAT. Additionally, noteworthy positive correlations were identified between urinary WT-1 and HbA1c levels, along with significant negative correlations between urinary WT-1 and eGFR at the 3, 6, 9, and 12-months post-KTx.</p></div><div><h3>Conclusion</h3><p>The increased urinary WT-1 levels from 3-months post-KTx in NODAT patients may indicate the first sign of podocyte injury, predicting a renal allograft dysfunction in these patients.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 153-159"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140136369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host genetic variants associated with COVID-19 reconsidered in a Slovak cohort","authors":"Maria Skerenova ,&nbsp;Michal Cibulka ,&nbsp;Zuzana Dankova ,&nbsp;Veronika Holubekova ,&nbsp;Zuzana Kolkova ,&nbsp;Vincent Lucansky ,&nbsp;Dana Dvorska ,&nbsp;Andrea Kapinova ,&nbsp;Michaela Krivosova ,&nbsp;Martin Petras ,&nbsp;Eva Baranovicova ,&nbsp;Ivana Baranova ,&nbsp;Elena Novakova ,&nbsp;Peter Liptak ,&nbsp;Peter Banovcin ,&nbsp;Anna Bobcakova ,&nbsp;Robert Rosolanka ,&nbsp;Maria Janickova ,&nbsp;Andrea Stanclova ,&nbsp;Ludovit Gaspar ,&nbsp;Erika Halasova","doi":"10.1016/j.advms.2024.03.007","DOIUrl":"10.1016/j.advms.2024.03.007","url":null,"abstract":"<div><p>We present the results of an association study involving hospitalized coronavirus disease 2019 (COVID-19) patients with a clinical background during the 3rd pandemic wave of COVID-19 in Slovakia. Seventeen single nucleotide variants (SNVs) in the eleven most relevant genes, according to the COVID-19 Host Genetics Initiative, were investigated. Our study confirms the validity of the influence of <em>LZTFL1</em> and 2′-5′-oligoadenylate synthetase (<em>OAS)1/OAS3</em> genetic variants on the severity of COVID-19. For two <em>LZTFL1</em> SNVs in complete linkage disequilibrium, rs17713054 and rs73064425, the odds ratios of baseline allelic associations and logistic regressions (LR) adjusted for age and sex ranged in the four tested designs from 2.04 to 2.41 and from 2.05 to 3.98, respectively. The <em>OAS1/OAS3</em> haplotype ‘gttg’ carrying a functional allele G of splice-acceptor variant rs10774671 manifested its protective function in the Delta pandemic wave. Significant baseline allelic associations of two <em>DPP9</em> variants in all tested designs and two <em>IFNAR2</em> variants in the Omicron pandemic wave were not confirmed by adjusted LR. Nevertheless, adjusted LR showed significant associations of <em>NOTCH4</em> rs3131294 and <em>TYK2</em> rs2304256 variants with severity of COVID-19. Hospitalized patients' reported comorbidities were not correlated with genetic variants, except for obesity, smoking (<em>IFNAR2</em>), and hypertension (<em>NOTCH4</em>). The results of our study suggest that host genetic variations have an impact on the severity and duration of acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Considering the differences in allelic associations between pandemic waves, they support the hypothesis that every new SARS-CoV-2 variant may modify the host immune response by reconfiguring involved pathways.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 198-207"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of NMDA receptors block versus GABA-A receptors modulation on synaptic plasticity and brain electrical activity in metabolic syndrome","authors":"Shaimaa Nasr Amin ,&nbsp;Sherif Ahmed Shaltout ,&nbsp;Walaa Bayoumie El Gazzar ,&nbsp;Noha Samir Abdel Latif ,&nbsp;Ghadah Nazar Al-jussani ,&nbsp;Yasmeen Jamal Alabdallat ,&nbsp;Khaled Anwer Albakri ,&nbsp;Dalia Azmy Elberry","doi":"10.1016/j.advms.2024.03.008","DOIUrl":"10.1016/j.advms.2024.03.008","url":null,"abstract":"<div><h3>Purpose</h3><p>Metabolic syndrome (MetS) is a common disorder associated with disturbed neurotransmitter homeostasis. Memantine, an <em>N</em>-methyl-<span>d</span>-aspartate receptor (NMDAR) antagonist, was first used in Alzheimer's disease. Allopregnanolone (Allo), a potent positive allosteric modulator of the Gamma-Amino-Butyric Acid (GABA)-A receptors, decreases in neurodegenerative diseases. The study investigated the impact of Memantine versus Allo administration on the animal model of MetS to clarify whether the mechanism of abnormalities is related more to excitatory or inhibitory neurotransmitter dysfunction.</p></div><div><h3>Materials and methods</h3><p>Fifty-six male rats were allocated into 7 groups: 4 control groups, 1 MetS group, and 2 treated MetS groups. They underwent assessment of cognition-related behavior by open field and forced swimming tests, electroencephalogram (EEG) recording, serum markers confirming the establishment of MetS model and hippocampal Glial Fibrillary Acidic Protein (GFAP) and Brain-Derived Neurotrophic Factor (BDNF).</p></div><div><h3>Results</h3><p>Allo improved anxiety-like behavior and decreased grooming frequency compared to Memantine. Both drugs increased GFAP and BDNF expression, improving synaptic plasticity and cognition-related behaviors. The therapeutic effect of Allo was more beneficial regarding lipid profile and anxiety. We reported progressive slowing of EEG waves in the MetS group with Memantine and Allo treatment with increased relative theta and decreased relative delta rhythms.</p></div><div><h3>Conclusions</h3><p>Both Allo and Memantine boosted the outcome parameters in the animal model of MetS. Allo markedly improved the anxiety-like behavior in the form of significantly decreased grooming frequency compared to the Memantine-treated groups. Both drugs were associated with increased hippocampal GFAP and BDNF expression, indicating an improvement in synaptic plasticity and so, cognition-related behaviors.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 1","pages":"Pages 176-189"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}