Advances in Clinical and Experimental Medicine最新文献

{"title":"Impact of magnesium on intraperitoneal adhesion in an experimental rat model.","authors":"Hakan Balbaloglu, Emre Isik, Ilhan Tasdoven, Inci Turan, Guldeniz Karadeniz Cakmak","doi":"10.17219/acem/187876","DOIUrl":"10.17219/acem/187876","url":null,"abstract":"<p><strong>Background: </strong>Intraperitoneal adhesions are fibrous bands that form between tissues and organs in the abdominal cavity, which can result from the body's healing process after surgery, leading to pain, bowel obstruction, and infertility in severe cases. Magnesium (Mg), known for its anti-inflammatory and anticoagulant properties, has been hypothesized to influence adhesion formation.</p><p><strong>Objectives: </strong>This study is designed to explore the hypothesized benefits of Mg, known for its anti-inflammatory and anticoagulant properties, on the prevention of intraperitoneal adhesions that commonly occur following abdominal surgeries. It seeks to provide a comprehensive understanding of Mg's potential role in mitigating adhesion formation, aiming to contribute valuable insights into postoperative recovery processes and outcomes.</p><p><strong>Material and methods: </strong>We employed an experimental model of intestinal abrasion in male Wistar rats. The rats were categorized into control and treatment groups, with the latter receiving varying doses of Mg sulfate. Intraperitoneal adhesions were induced using a multi-abrasion model.</p><p><strong>Results: </strong>Based on both the Evans model and histopathological evaluations, it was observed that there were significant differences in adhesion scores between the groups. Magnesium-treated groups showed significantly fewer adhesions than the control group. Histopathological analyses indicated variations in adhesion characteristics and inflammatory responses among the groups.</p><p><strong>Conclusions: </strong>Preliminary results indicated the potential role of Mg in mitigating postoperative intraperitoneal adhesions. These findings suggest the need for further research to confirm the efficacy of Mg and to explore its mechanisms of action in clinical settings.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"777-785"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An umbrella analysis assessing the risk of acute kidney injury in COVID-19 patients.","authors":"Qinyan Gu, Yongzhen Zhang, Xijun He","doi":"10.17219/acem/187998","DOIUrl":"10.17219/acem/187998","url":null,"abstract":"<p><p>A number of research studies have indicated a potential association between COVID-19 and acute kidney injury (AKI). However, the methodologies employed and the risk estimates derived from these studies vary. Therefore, an umbrella review of systematic reviews and meta-analyses was conducted to determine the incidence of AKI in COVID-19 patients and AKI-associated mortality. A complete literature search was undertaken in PubMed, Embase, Scopus, and the Cochrane Library. The methodological rigor of the included papers was evaluated using the Assessment of Multiple Systematic Reviews (AMSTAR-2) instrument. The pooled risk ratio (RR) and odds ratio (OR) of the included studies were calculated to establish the strength of the association between AKI cases and COVID-19 infections. This umbrella review included 20 studies. Two of the 20 studies assessed adult COVID-19 patient risk factors for AKI, 1 examined survival rates and 7 examined the incidence of AKI. The remaining 10 investigations revealed that patients with coronavirus were susceptible to AKI. The umbrella analysis comprised reviews that contained a range of 6 to 54 papers. The AMSTAR-2 ratings yielded a total of 14 studies deemed to be of high quality, with 6 studies classified as intermediate quality. Statistical analysis of included reviews revealed a 1.50 RR for AKI incidence in COVID-19 patients (95% confidence interval (95% CI): 1.40-1.60, I2 69%, p < 0.0001) and a 2.02 RR (95% CI: 1.79-2.29, I2 56%, p < 0.0001) for AKI-associated death. This umbrella review revealed that individuals infected with the novel coronavirus often develop AKI. SARS-CoV-2 infections were associated with AKI due to advanced age, male gender, coronary artery disease, diabetes, and hypertension. However, AKI and a renal replacement therapy (RRT) requirement independently predicted unfavorable COVID-19 results.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"831-845"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis of the clinical significance of the TKI-resistant gene ZNF687 for hepatocellular carcinoma patients.","authors":"Guan-Lan Zhang, Jian-Di Li, Ji-Feng He, Kun-Jun Wu, Ying-Yu Mo, Song-Yang Zhong, Xuan-Fei Wang, Fei-Fei Wu, Yi-Si Qin, Hong Zhao, Zhi-Guang Huang, Gang Chen, Rong-Quan He","doi":"10.17219/acem/188425","DOIUrl":"10.17219/acem/188425","url":null,"abstract":"<p><strong>Background: </strong>Novel treatments such as monotherapy and combined immunotherapy significantly extend overall survival (OS) for hepatocellular carcinoma (HCC) patients, but HCC is susceptible to treatment resistance during long-term therapy. The resistance mechanism to targeted drugs in HCC remains ambiguous, making research on HCC drug resistance targets crucial for the development of precision medicine.</p><p><strong>Objectives: </strong>To investigate the transcriptional features, biological functions and potential clinical value of the tyrosine kinase inhibitor (TKI)-resistant gene ZNF687 in HCC.</p><p><strong>Material and methods: </strong>The TKI-resistant genes of HCC were identified using clustered regularly interspaced short palindromic repeats (CRISPR) in vitro screening. Then, the dependence of HCC cell lines on ZNF687 was investigated in silico. We collected global mRNA datasets of HCC tissue, integrated the mRNA expression characteristics of ZNF687 in HCC and explored the impact of ZNF687 on HCC patient prognoses using the Kaplan-Meier method (in silico). The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses were then conducted, and a connectivity map and molecular docking technology were applied to find the underlying agent opposing ZNF687.</p><p><strong>Results: </strong>In vitro, the guide RNA corresponding to ZNF687 was weakly detected in HCC cells, and ZNF687 deficiency was found to inhibit growth in HCC cell lines. ZNF687 mRNA was overexpressed and had a high discriminatory ability for HCC in 2,975 HCC samples, contrasting with 2,340 non-HCC samples. Moreover, an excessive ZNF687 transcript level was related to a worse overall survival (OS) prognosis. Histone modification, spliceosome, transcription coregulator activity, and nucleocytoplasmic transport were the most significant pathways for ZNF687 differential-related gene enrichment. Chaetocin was found to be a candidate compound and presented a strong affinity to ZNF687.</p><p><strong>Conclusions: </strong>ZNF687 represents a TKI-resistant and growth-dependent gene for HCC, the overexpression of which indicates poor OS for HCC patients. Additionally, ZNF687 is expected to be a druggable target for overcoming TKI resistance, and chaetocin may be a candidate therapeutic compound for ZNF687.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"787-801"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between rheumatoid arthritis and epicardial fat thickness, and serum levels of chemerin, adropin, and betatrophin.","authors":"Bilge Ekinci, Cuma Mertoglu, Resit Coskun, Yusuf Kemal Arslan, Taha Abdulkadir Coban, Fatih Ozcicek","doi":"10.17219/acem/190059","DOIUrl":"10.17219/acem/190059","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular diseases (CVDs) are highly prevalent among patients with rheumatoid arthritis (RA). Epicardial adipose tissue, serum betatrophin, chemerin, and adropin levels are factors associated with atherosclerosis and cardiovascular involvement.</p><p><strong>Objectives: </strong>This study aimed to investigate the relationship between RA and epicardial fat thickness (EFT), as well as serum betatrophin, chemerin and adropin levels.</p><p><strong>Material and methods: </strong>This cross-sectional study included 80 patients (62 women and 18 men) diagnosed with RA according to the American College of Rheumatology/The European Alliance of Associations for Rheumatology (ACR/EULAR) 2010 RA classification criteria and 80 healthy controls (64 women and 16 men). Exclusion criteria comprised other autoimmune diseases, CVDs, diabetes mellitus, other endocrine disorders, acute or chronic pancreatic disorders, malignancy, pregnancy, breastfeeding, or antihyperlipidemic drug usage. Serum betatrophin, chemerin and adropin concentrations were measured. Epicardial fat thickness was evaluated with transthoracic echocardiography.</p><p><strong>Results: </strong>Adropin levels were significantly lower in the RA group compared to the control group (p < 0.001). Chemerin levels and EFT were significantly higher in the RA group than in the control group (p = 0.016, p < 0.001, respectively). When assessing the relationship between biomarkers and EFT in RA patients, a strong positive correlation was observed between chemerin and EFT (r = 0.73, p = 0.046) in patients with high disease activity.</p><p><strong>Conclusions: </strong>Epicardial fat thickness, as an indicator of cardiovascular involvement, is higher in patients with RA. Moreover, high chemerin levels and low adropin levels in these patients may be indicative of cardiovascular involvement.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"709-715"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of systemic and topical antimicrobial therapy combined with non-surgical periodontal therapy: A meta-analysis.","authors":"Guangping Sun","doi":"10.17219/acem/188258","DOIUrl":"10.17219/acem/188258","url":null,"abstract":"<p><strong>Background: </strong>Combining antibiotics with non-surgical periodontal therapy has a beneficial impact in case of infection while its role for dental-related outcomes is still unclear.</p><p><strong>Objectives: </strong>The current study's main objective was to evaluate the impact of adding adjuvant systemic and topical antimicrobial therapy to non-surgical periodontal therapy.</p><p><strong>Material and methods: </strong>A systematic literature search was accomplished and 1,093 study participants with periodontal diseases were recruited to the current study; 541 of them were treated with adjuvant systemic or topical antimicrobial agents and 552 with non-surgical interventions. The inclusion criteria of the current study took into account only randomized clinical trials.</p><p><strong>Results: </strong>Adding systemic antibiotics to non-surgical intervention resulted in a significant enhancement regarding probing pocket depth reduction (PPD). Metronidazole/amoxicillin showed a significant impact on PPD and the clinical attachment level (CAL), while doxycycline showed no significant impact regarding CAL. Using topical antimicrobial agents showed a significant beneficial role in reducing PPD regarding doxycycline, while non-significant effects were seen with metronidazole.</p><p><strong>Conclusion: </strong>Adding adjuvant systemic and topical antimicrobial agents to non-surgical periodontal therapy showed a beneficial impact regarding PPD and CAL (metronidazole/amoxicillin and doxycycline). In addition, using doxycycline as a topical agent showed a beneficial impact on the reduction of PPD.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"681-692"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the use of an antigravity device in leveling functional inequalities of the lower limbs and inhibiting the progression of idiopathic scoliosis.","authors":"Marek Kluszczyński, Ilona Karpiel, Aneta Piechaczek","doi":"10.17219/acem/191598","DOIUrl":"10.17219/acem/191598","url":null,"abstract":"<p><strong>Background: </strong>The conservative treatment of idiopathic scoliosis (IS) may be enhanced through a combination of specialized physiotherapy, bracing, and the utilization of assistive devices.</p><p><strong>Objectives: </strong>This study aims to evaluate the efficacy of the GraviSpine device in supporting the conservative treatment of IS in children.</p><p><strong>Material and methods: </strong>A cohort of 142 patients, aged 10-17 years with an average age of 12.76 ±1.75 years, undergoing treatment for IS with specific physiotherapy and bracing, received additional treatment with the GraviSpine device. The participants, selected based on inclusion and exclusion criteria, were divided into 2 age groups: group A (10-12 years) and group B (13-17 years). The mean follow-up period was 28.71 ±10.98 months. The assessment involved changes in post-treatment trunk rotation angles (ATR), Cobb angles, and functional lower limb length discrepancies (FLLDs) concerning age groups and scoliosis location.</p><p><strong>Results: </strong>The proportion of patients showing improvement and stabilization was high in both groups A and B, at 71% and 90%, respectively. In group B, a significant reduction in the mean Cobb angle of -1.83° ±6.88°, p < 0.002, was observed. Furthermore, a significant decrease in the incidence of FLLDs was noted in thoracic and lumbar scoliosis locations, p < 0.002.</p><p><strong>Conclusions: </strong>To enhance the effectiveness of conservative treatment for IS, the utilization of an assistive device such as GraviSpine may be considered, particularly when the child presents functional inequality of the lower limbs.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"737-746"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of education and mental health of pharmacy students in Poland.","authors":"Dorota Bęben, Monika Birska, Łukasz Gądek, Dominik Marciniak, Katarzyna Karłowicz-Bodalska","doi":"10.17219/acem/189228","DOIUrl":"10.17219/acem/189228","url":null,"abstract":"<p><strong>Background: </strong>Pharmacists in the healthcare system play an important role in providing safe, optimal pharmacotherapy and patient education. During their studies, in which they acquire the competencies to fulfill the pharmacist's future tasks, pharmacy students are exposed to significant stress and pressure.</p><p><strong>Objectives: </strong>This study aims to demonstrate the extent to which the unique demands and obstacles of Polish pharmacy schools contribute to the deterioration of students' mental health and overall wellbeing.</p><p><strong>Material and methods: </strong>A cross-sectional study of 420 pharmacy students in Poland evaluates the quality of education at Polish universities and presents the impact of studying on students' mental health. The criteria for choosing the field of study, the particular major, the university itself, the quality of education, the academic work, and their impact on students' wellbeing were evaluated. The evaluation of the quality of education was influenced by mentoring and tutoring at the university. Pearson's ÷2 test and principal component analysis (PCA) were used in the statistical analyses.</p><p><strong>Results: </strong>Unequal treatment of pharmacy students relative to students in other areas of medical study was marked by 90.2% of respondents, and opportunities for scientific development were indicated as good by 60.0% of pharmacy students. It was shown that 82.1% of the students rated studying as very stressful; the level of test difficulty and exams, as well as an inadequate level of knowledge imparted during classes contributed to this response. According to 75.2% of the respondents, the perceived stress had long-term effects in the form of anxiety and depression, with the need for pharmacotherapy.</p><p><strong>Conclusions: </strong>It was shown that studies contributed to the onset and/or exacerbation of depressive and anxiety symptoms. The results indicate the need to support psychological care and extend it to to pharmacy students.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"753-762"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing cancer treatment: Navigating the intricate landscape of cellular signaling networks.","authors":"Hao Zhang","doi":"10.17219/acem/205024","DOIUrl":"10.17219/acem/205024","url":null,"abstract":"<p><p>Cancer progression and therapeutic resistance are propelled by the remarkable plasticity of signaling networks, which dynamically rewire under selective pressures to maintain proliferation, enable immune evasion and promote metastasis. Despite advances in precision oncology, the dynamic crosstalk between tumor cells, non-coding genomes and the microenvironment continues to undermine treatment efficacy. This call for submissions, Revolutionizing Cancer Treatment: Navigating the Intricate Landscape of Cellular Signaling Networks, seeks cutting-edge research that dissects these adaptive mechanisms through innovative technologies - from single-cell multi-omics and spatial transcriptomics to AI-powered network modeling. We welcome studies leveraging physiomimetic models (e.g., organoids, 3D-bioprinted ecosystems) to decode tumor heterogeneity, as well as translational work targeting emergent vulnerabilities at the intersection of epigenetics, metabolic reprogramming and stromal interactions. By integrating systems biology with computational and experimental approaches, this collection aims to catalyze the design of adaptive therapies that outmaneuver cancer's evolutionary resilience.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"669-671"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrition-related needs and considerations in the transgender and gender non-conforming (TGNC) population: Current gaps and future directions in research.","authors":"Anthony Dissen, Christina Riccardo","doi":"10.17219/acem/204177","DOIUrl":"10.17219/acem/204177","url":null,"abstract":"<p><p>Transgender and gender non-conforming (TGNC) individuals face significant health disparities despite growing visibility and awareness. Nutrition-related disparities are particularly concerning, with TGNC individuals experiencing higher rates of food insecurity, eating disorders, body dissatisfaction, and overweight or obesity. Gender-affirming hormone therapy (GAHT) and other medical interventions lead to significant physiological changes that can influence nutritional needs, as well as body composition and bone mineral density, yet existing daily energy estimation equations do not account for TGNC individuals or those undergoing GAHT. Perioperative nutrition is also vital, as gender-affirming surgeries increase metabolic demands and risk of muscle loss due to immobility and catabolism. Moreover, TGNC individuals report higher rates of food insecurity, with transgender men being particularly affected, largely due to economic disparities and systemic discrimination. Eating disorders are likely under reported in this population due to limited access to competent, inclusive care. Most research combines transgender and gender non-conforming individuals, limiting insight into subgroup differences. To improve health outcomes among TGNC individuals, there is a critical need for more inclusive, longitudinal research, particularly in the areas of nutrition, metabolism, and post-surgical care. Such research could inform the development of tailored interventions, enhance healthcare provider competence, and support the creation of clinical guidelines that address the specific health and nutrition needs of TGNC individuals. Ultimately, this would help reduce disparities and promote long-term well-being for TGNC individuals.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"663-667"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematologists' state of knowledge on practical aspects of hemophagocytic lymphohistiocytosis (HLH) in adult patients: A Young Hematologists' Club survey.","authors":"Magdalena Witkowska, Przemysław Kasiak, Kamila Kruczkowska-Tarantowicz, Marcin Jońca, Agnieszka Walczyk, Michał Górka, Monika Sidor, Bartosz Puła, Rafał Machowicz","doi":"10.17219/acem/189945","DOIUrl":"10.17219/acem/189945","url":null,"abstract":"<p><strong>Background: </strong>Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening hyperinflammatory syndrome with various etiologies. Its treatment is complicated by several important but not immediately obvious issues (e.g., HLH-2004 criteria are the most commonly used for diagnosis, but the recommended therapeutic regimen is HLH-94).</p><p><strong>Objectives: </strong>The study aimed to assess hematologists' practical knowledge of HLH.</p><p><strong>Material and methods: </strong>A survey was conducted among physicians treating adult hematological patients. A 10-question paper questionnaire was distributed to physicians from various hematology centers. A total of 126 questionnaires were analyzed.</p><p><strong>Results: </strong>Most respondents had little-to-moderate experience in caring for HLH patients: 59% treated 0-2 patients and 36% treated between 3-5 patients. Among the respondents, the preferred diagnostic criteria were HLH-2004, either in its original form (5 out of 8 criteria) for 70 respondents or its modified version (4 out of 6 available criteria when ferritin exceeds 2,000 ng/mL) for 56 respondents. The preferred treatment regimen was HLH-2004, with a full dose of etoposide in 72 responses or reduced in 39 responses. Fifty percent of respondents incorrectly answered that meeting the HLH-2004 criteria necessitates the use of the HLH-94/2004 regimen in full doses and duration. Sixty-four percent of respondents correctly identified that hemophagocytosis is not necessary for the diagnosis.</p><p><strong>Conclusions: </strong>This survey reveals that the majority of surveyed physicians adhere to international HLH recommendations. However, there are instances where these guidelines are not fully implemented, which underlines the need for further efforts to raise awareness and share clinical experiences about this patient group.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"747-752"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}