Advances in Clinical and Experimental Medicine最新文献

{"title":"Utilizing laser therapy to manage oral potentially malignant disorders in older adults at the primary care level.","authors":"Sajee Sattayut, Patcharawan Srisilapanan, Piyachat Patcharanuchat","doi":"10.17219/acem/193604","DOIUrl":"https://doi.org/10.17219/acem/193604","url":null,"abstract":"<p><p>Oral lesions are a significant concern among older adults because they can progress to oral cancer if not diagnosed and treated promptly and effectively. Transportation to the hospital is a major barrier to oral healthcare for many older adults. The purpose of this editorial is to address the challenges of managing oral potentially malignant disorders in the older population, highlighting the barriers they face in accessing healthcare services, the potential use of laser therapy for management, and the direction of research in this area. Due to the limited access of the older to healthcare services, primary healthcare facilities within communities serve as their primary providers. Laser therapy is recommended for the management of oral potentially malignant disorders due to its favorable outcomes. This approach has been tested in several primary healthcare centers in Thailand. In our project, laser therapy was used to treat oral potentially malignant disorders in primary and secondary healthcare services. This includes photodynamic therapy for older patients with extensive lesions, as well as individuals with oral leukoplakia and erythroplakia who have declined curative surgery. It has also been used in cases of recalcitrant lichen planus to steroid or photobiomodulation therapy. This approach has been well accepted by both oral healthcare providers and patients. To expand access to these treatment options in such settings, it is critical to empower healthcare professionals, particularly dentists and dental nurses, to integrate laser techniques into geriatric care and oral cancer screening. Establishing a network foundation for orofacial laserology would also enhance the potential of such settings.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":"33 10","pages":"1033-1037"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fc-gamma receptor expression and cytokine responses to intravenous human immunoglobulin in whole blood from non-pregnant and pregnant women and newborns.","authors":"Stephania Vázquez-Rodríguez, Lourdes A Arriaga-Pizano, Ismael Mancilla-Herrera, Jessica Prieto-Chávez, Roberto Arizmendi-Villanueva, Rafael Torres-Rosas, Ana Flisser, Ethel García-Latorre, Arturo Cérbulo-Vázquez","doi":"10.17219/acem/174566","DOIUrl":"10.17219/acem/174566","url":null,"abstract":"<p><strong>Background: </strong>Intravenous immunoglobulin (IVIG) can suppress the inflammatory response in adults, but its role in pregnant women and newborns is poorly studied. While the adult immune system is considered mature, it is immature in neonates and suppressed in pregnancy. Since the immune response differs in these 3 groups, the use of IVIG could differentially modulate the immune response.</p><p><strong>Objectives: </strong>We aimed to explore the effect of IVIG on myeloid blood cells from non-pregnant women, pregnant women and newborns.</p><p><strong>Material and methods: </strong>Whole blood from healthy donors was incubated with lipopolysaccharide (LPS) and/or IVIG. After 0 h, 24 h and 48 h of culture, Fc-gamma receptor (CD16, CD32 and CD64) expression, monocyte and neutrophil bacterial phagocytosis, and cytokine and chemokine concentrations were determined in the supernatant.</p><p><strong>Results: </strong>The baseline expression of monocyte CD16 was higher in newborns than in adult women, but the expression of CD32 and CD64 was similar between groups. Furthermore, LPS and IVIG stimulation, together or separately, did not change Fc-gamma receptor expression in monocytes or neutrophils and did not modify their phagocytosis capacity. On the other hand, IVIG did not downregulate the proinflammatory cytokine response induced by LPS in any group. Interestingly, IVIG induced a strong interleukin 8 (IL-8) response in neonates but not in non-pregnant or pregnant women.</p><p><strong>Conclusions: </strong>Our results show that IVIG did not induce changes in Fc-gamma receptor expression, phagocytic ability, or the cytokine response to LPS in blood cells from neonates, non-pregnant or pregnant women. However, IVIG induced a strong IL-8 response in neonates that could improve immunity.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"1115-1122"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The roles of autophagy in the treatment of diabetic nephropathy with rapamycin.","authors":"Ya Fu, Liang Zhang, Shupei Qin, Meng Tang, Yanxia Hao, Xuedong Chen, Yan Wang, Ting Zhou, Yuemei Xue, Long Cheng, Na Liu, Qifeng Jia, Yangyang Chen, Li Li","doi":"10.17219/acem/175776","DOIUrl":"10.17219/acem/175776","url":null,"abstract":"<p><strong>Background: </strong>Rapamycin is known to induce autophagy, promote cell survival and inhibit the progression of diabetic nephropathy (DN).</p><p><strong>Objectives: </strong>The aim of this study was to examine the role of autophagy in the treatment of DN with rapamycin to provide the basis for the DN treatment with rapamycin.</p><p><strong>Material and methods: </strong>Human mesangial cells (HMC) were cultured in a constant temperature incubator with 5% CO2, at 37°C and saturated humidity. Cells were divided into 5 groups and the 5-ethynyl-2-deoxyuridine (EdU) cell proliferation assay was used to determine cell proliferation. Flow cytometry was used to determine cell apoptosis, while GFP-RFP-LC3 showed autophagy flow. Western blot was employed to detect the expression of autophagy-related proteins LC3-II/LC3-I and P62. Enzyme-linked immunosorbent assay (ELISA) was used to determine the contents of type IV collagen fiber (Col4), hyaluronic acid (HA) and laminin (LA) in the extracellular matrix (ECM).</p><p><strong>Results: </strong>Cell proliferation was the lowest in the hyperglycemic group. Additionally, the hyperglycemic group displayed the lowest number of autolysosomes compared to other groups. In contrast, the rapamycin group exhibited the highest number of autolysosomes. The LC3-II/LC3-I ratio was also the lowest in the hyperglycemic group, measuring 0.53 (0.50-0.58), while the expression level of P62 was significantly higher in that group at 0.98 (0.95-1.01) compared to other groups. Upon the introduction of rapamycin, the LC3-II/LC3-I ratio was significantly increased at 2.21 (1.95-2.21), and P62 was significantly decreased 0.38 (0.38-0.39) compared to the hyperglycemic group. Both changes were statistically significant, with p-values of 0.034 and 0.010, respectively. Enzyme-linked immunosorbent assay was employed to detect Col4, HA and LA content. The study findings demonstrated significantly higher levels of glucose in the hyperglycemic group in comparison to other groups. In contrast, the rapamycin group exhibited significantly lower levels of glucose than the hyperglycemic group, yet the difference was not statistically significant.</p><p><strong>Conclusions: </strong>Hyperglycemic can inhibit the autophagic activity of HMC, promote cell apoptosis, enhance ECM accumulation, and facilitate the DN progression. In contrast, rapamycin can elicit autophagy, decrease mesangial matrix proliferation, and therefore impede DN progression.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"1141-1152"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139690968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contemporary methods of treating venous lake lesions on the oral mucosa: A literature review.","authors":"Mateusz Trafalski, Kamil Jurczyszyn","doi":"10.17219/acem/188464","DOIUrl":"https://doi.org/10.17219/acem/188464","url":null,"abstract":"<p><p>A venous lake (VL) is a vascular lesion arising from dilated venous vessels surrounded by thick fibrous tissue, located in the upper layers of the dermis. It can also appear in the oral cavity, especially on the lips, buccal mucosa and tongue. Recurrent bleeding or aesthetic complaints are the most common reasons for the treatment of these lesions. This review aims to present the current state of knowledge regarding the treatment of VL lesions in the oral cavity. PRISMA guidelines were followed. Articles were searched in the following databases: Pubmed, Medline and Scopus. The authors of this study analyzed scientific works concerning VL treatment. Keywords searched included \"venous lake\", \"venous lake treatment\", \"sclerotherapy\", \"laser\", \"laser photocoagulation\", \"infrared coagulation\", and \"diathermocoagulation\". Two articles described electrocoagulation, 10 articles focused on photocoagulation using laser devices, 2 articles studied photocoagulation with infrared, and 4 articles described sclerotherapy for the treatment of VL lesions. The most effective therapeutic options were electrocoagulation, 808 nm diode laser photocoagulation and 1064 nm Nd:YAG.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between rheumatoid arthritis and epicardial fat thickness, and serum levels of chemerin, adropin, and betatrophin.","authors":"Bilge Ekinci, Cuma Mertoglu, Resit Coskun, Yusuf Kemal Arslan, Taha Abdulkadir Coban, Fatih Ozcicek","doi":"10.17219/acem/190059","DOIUrl":"https://doi.org/10.17219/acem/190059","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular diseases (CVDs) are highly prevalent among patients with rheumatoid arthritis (RA). Epicardial adipose tissue, serum betatrophin, chemerin, and adropin levels are factors associated with atherosclerosis and cardiovascular involvement.</p><p><strong>Objectives: </strong>This study aimed to investigate the relationship between RA and epicardial fat thickness (EFT), as well as serum betatrophin, chemerin and adropin levels.</p><p><strong>Material and methods: </strong>This cross-sectional study included 80 patients (62 women and 18 men) diagnosed with RA according to the American College of Rheumatology/The European Alliance of Associations for Rheumatology (ACR/EULAR) 2010 RA classification criteria and 80 healthy controls (64 women and 16 men). Exclusion criteria comprised other autoimmune diseases, CVDs, diabetes mellitus, other endocrine disorders, acute or chronic pancreatic disorders, malignancy, pregnancy, breastfeeding, or antihyperlipidemic drug usage. Serum betatrophin, chemerin and adropin concentrations were measured. Epicardial fat thickness was evaluated with transthoracic echocardiography.</p><p><strong>Results: </strong>Adropin levels were significantly lower in the RA group compared to the control group (p < 0.001). Chemerin levels and EFT were significantly higher in the RA group than in the control group (p = 0.016, p < 0.001, respectively). When assessing the relationship between biomarkers and EFT in RA patients, a strong positive correlation was observed between chemerin and EFT (r = 0.73, p = 0.046) in patients with high disease activity.</p><p><strong>Conclusions: </strong>Epicardial fat thickness, as an indicator of cardiovascular involvement, is higher in patients with RA. Moreover, high chemerin levels and low adropin levels in these patients may be indicative of cardiovascular involvement.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel strategies of glutathione depletion in photodynamic and chemodynamic therapy: A review.","authors":"Daniel Wolny, Mateusz Stojko, Alicja Zajdel","doi":"10.17219/acem/191025","DOIUrl":"https://doi.org/10.17219/acem/191025","url":null,"abstract":"<p><p>Cancer remains a health problem worldwide; therefore, developing new therapies to increase the effectiveness of anticancer treatments is necessary. Two such methods are photodynamic therapy (PDT) and chemodynamic therapy (CDT). The intensive growth and increased metabolism of tumors lead to elevated levels of reactive oxygen species (ROS) within cancer cells. These cells develop several antioxidant mechanisms to protect them from this oxidative stress. Antioxidants also make tumors more resistant to chemotherapy and radiation. Glutathione (GSH) is an important and the most abundant endogenous cellular antioxidant. Photodynamic therapy and CDT are new methods that are based on the production of ROS,‑ therefore increasing oxidative stress in cancer cells. A significant problem with these therapies is the increased GSH levels, which is an adaptation of cancer cells to augmented metabolic processes. This paper presents various GSH depletion strategies that are used to improve PDT and CDT. While the main goal of GSH depletion in both PDT and CDT is to prevent its interaction with the ROS generated by these therapies, it should be remembered that the reduction of its level itself may initiate pathways leading to cancer cell death.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of magnesium on intraperitoneal adhesion in an experimental rat model.","authors":"Hakan Balbaloglu, Emre Isik, Ilhan Tasdoven, Inci Turan, Guldeniz Karadeniz Cakmak","doi":"10.17219/acem/187876","DOIUrl":"https://doi.org/10.17219/acem/187876","url":null,"abstract":"<p><strong>Background: </strong>Intraperitoneal adhesions are fibrous bands that form between tissues and organs in the abdominal cavity, which can result from the body's healing process after surgery, leading to pain, bowel obstruction, and infertility in severe cases. Magnesium (Mg), known for its anti-inflammatory and anticoagulant properties, has been hypothesized to influence adhesion formation.</p><p><strong>Objectives: </strong>This study is designed to explore the hypothesized benefits of Mg, known for its anti-inflammatory and anticoagulant properties, on the prevention of intraperitoneal adhesions that commonly occur following abdominal surgeries. It seeks to provide a comprehensive understanding of Mg's potential role in mitigating adhesion formation, aiming to contribute valuable insights into postoperative recovery processes and outcomes.</p><p><strong>Material and methods: </strong>We employed an experimental model of intestinal abrasion in male Wistar rats. The rats were categorized into control and treatment groups, with the latter receiving varying doses of Mg sulfate. Intraperitoneal adhesions were induced using a multi-abrasion model.</p><p><strong>Results: </strong>Based on both the Evans model and histopathological evaluations, it was observed that there were significant differences in adhesion scores between the groups. Magnesium-treated groups showed significantly fewer adhesions than the control group. Histopathological analyses indicated variations in adhesion characteristics and inflammatory responses among the groups.</p><p><strong>Conclusions: </strong>Preliminary results indicated the potential role of Mg in mitigating postoperative intraperitoneal adhesions. These findings suggest the need for further research to confirm the efficacy of Mg and to explore its mechanisms of action in clinical settings.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethoxyquin mediates lung fibrosis and cellular immunity in BLM-CIA mice by inhibiting HSP90.","authors":"Jie-Rou Huang, Liang Chen, Chao-Qian Li","doi":"10.17219/acem/186365","DOIUrl":"https://doi.org/10.17219/acem/186365","url":null,"abstract":"<p><strong>Background: </strong>Patients with rheumatoid arthritis-associated interstitial lung disease (RA-ILD) are characterized by severe pulmonary fibrosis and immune dysregulation. Heat shock protein 90 (HSP90) is involved in the progression of pulmonary fibrosis and the immune response.</p><p><strong>Objectives: </strong>This study aimed to explore whether HSP90 regulates the development of RA-ILD and its underlying mechanism.</p><p><strong>Material and methods: </strong>In vivo, collagen-induced arthritis (CIA)-mice were treated with bleomycin (BLM) to establish an arthritic mouse model of pulmonary fibrosis. In vitro, human lung fibroblast 1 (HLF1) was exposed to transforming growth factor beta 1 (TGF-β1) to simulate an RA-ILD model. The RA-ILD models were treated with the HSP90 inhibitor ethoxyquin (EQ) to explore the potential mechanism of HSP90 in RA-ILD. Histopathological analysis was performed, and pulmonary fibrosis was evaluated. The differentiation of M1/M2 macrophages and Th1/Th17/Treg cells was assessed. The role of the TGF-β/Smad2/3 pathway in EQ-mediated RA-ILD progression was also explored.</p><p><strong>Results: </strong>HSP90α and HSP90β were upregulated in the RA-ILD models. Ethoxyquin mitigated arthritis in BLM-CIA mice, and reduced the expression of alpha-smooth muscle actin (α-SMA), collagen I (Col-1) and fibronectin (FN), as well as hydroxyproline content, thereby relieving pulmonary fibrosis. In addition, EQ increased M1 macrophages and inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNF-α) levels; conversely, EQ decreased M2 macrophages and vascular endothelial growth factor (VEGF)-A and TGF-β1 contents. It also decreased Th17 (interleukin (IL)-17) while increasing Th1 (interferon gamma (IFN-γ)) and Treg (Foxp3), and restricted the expression of transforming growth factor beta type receptor I and II (TGF-βRI and TGF-βRII) and the phosphorylation of Smad2 and Smad3.</p><p><strong>Conclusions: </strong>This study revealed that EQ regulated pulmonary fibrosis and cellular immunity by inhibiting HSP90, appearing to act through the TGF-β/Smad2/3 pathway. These findings suggest that EQ holds potential as a therapeutic agent for treating RA-ILD.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of systemic and topical antimicrobial therapy combined with non-surgical periodontal therapy: A meta-analysis.","authors":"Guangping Sun","doi":"10.17219/acem/188258","DOIUrl":"https://doi.org/10.17219/acem/188258","url":null,"abstract":"<p><strong>Background: </strong>Combining antibiotics with non-surgical periodontal therapy has a beneficial impact in case of infection while its role for dental-related outcomes is still unclear.</p><p><strong>Objectives: </strong>The current study's main objective was to evaluate the impact of adding adjuvant systemic and topical antimicrobial therapy to non-surgical periodontal therapy.</p><p><strong>Material and methods: </strong>A systematic literature search was accomplished and 1,093 study participants with periodontal diseases were recruited to the current study; 541 of them were treated with adjuvant systemic or topical antimicrobial agents and 552 with non-surgical interventions. The inclusion criteria of the current study took into account only randomized clinical trials.</p><p><strong>Results: </strong>Adding systemic antibiotics to non-surgical intervention resulted in a significant enhancement regarding probing pocket depth reduction (PPD). Metronidazole/amoxicillin showed a significant impact on PPD and the clinical attachment level (CAL), while doxycycline showed no significant impact regarding CAL. Using topical antimicrobial agents showed a significant beneficial role in reducing PPD regarding doxycycline, while non-significant effects were seen with metronidazole.</p><p><strong>Conclusion: </strong>Adding adjuvant systemic and topical antimicrobial agents to non-surgical periodontal therapy showed a beneficial impact regarding PPD and CAL (metronidazole/amoxicillin and doxycycline). In addition, using doxycycline as a topical agent showed a beneficial impact on the reduction of PPD.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of education and mental health of pharmacy students in Poland.","authors":"Dorota Bęben, Monika Birska, Łukasz Gądek, Dominik Marciniak, Katarzyna Karłowicz-Bodalska","doi":"10.17219/acem/189228","DOIUrl":"https://doi.org/10.17219/acem/189228","url":null,"abstract":"<p><strong>Background: </strong>Pharmacists in the healthcare system play an important role in providing safe, optimal pharmacotherapy and patient education. During their studies, in which they acquire the competencies to fulfill the pharmacist's future tasks, pharmacy students are exposed to significant stress and pressure.</p><p><strong>Objectives: </strong>This study aims to demonstrate the extent to which the unique demands and obstacles of Polish pharmacy schools contribute to the deterioration of students' mental health and overall wellbeing.</p><p><strong>Material and methods: </strong>A cross-sectional study of 420 pharmacy students in Poland evaluates the quality of education at Polish universities and presents the impact of studying on students' mental health. The criteria for choosing the field of study, the particular major, the university itself, the quality of education, the academic work, and their impact on students' wellbeing were evaluated. The evaluation of the quality of education was influenced by mentoring and tutoring at the university. Pearson's ÷2 test and principal component analysis (PCA) were used in the statistical analyses.</p><p><strong>Results: </strong>Unequal treatment of pharmacy students relative to students in other areas of medical study was marked by 90.2% of respondents, and opportunities for scientific development were indicated as good by 60.0% of pharmacy students. It was shown that 82.1% of the students rated studying as very stressful; the level of test difficulty and exams, as well as an inadequate level of knowledge imparted during classes contributed to this response. According to 75.2% of the respondents, the perceived stress had long-term effects in the form of anxiety and depression, with the need for pharmacotherapy.</p><p><strong>Conclusions: </strong>It was shown that studies contributed to the onset and/or exacerbation of depressive and anxiety symptoms. The results indicate the need to support psychological care and extend it to to pharmacy students.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}