Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)最新文献_第3页

MODULATION OF THE MONOSYNAPTIC REFLEX POTENTIALSIN THE DECEREBRATED RATS UNDER THE INFLUENCE OF HYDROXYTRYPTOPHAN. 羟色氨酸对失脑大鼠单突触反射电位的调节作用。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.041

I V Vereshchaka, N V Bulgakova, V V Kornieiev, K A Chaikovskii, A V Maznychenko

{"title":"MODULATION OF THE MONOSYNAPTIC REFLEX POTENTIALSIN THE DECEREBRATED RATS UNDER THE INFLUENCE OF HYDROXYTRYPTOPHAN.","authors":"I V Vereshchaka, N V Bulgakova, V V Kornieiev, K A Chaikovskii, A V Maznychenko","doi":"10.15407/fz62.02.041","DOIUrl":"https://doi.org/10.15407/fz62.02.041","url":null,"abstract":"We studied the serotonin effect on monosynaptic reflex potentials (MSR) of spinal motorneurons in the decerebrated rats in control and after intraperitoneal administration of serotonin precursor – 5-Hydroxytryptophan (5-HTP). MSR of motorneurons in the lumbar spinal cord were registered using electrical stimulation of dorsal root of the 5th lumbar section. During stimulation physiological saline or 5-hydroxytryptophan was injected intraperitoneally. In comparison with average amplitude of the control MSR there were registered significant increase in amplitudes of the MSR (169% and +172%, P <0,001) in animals with injection 5-HTP. These data suggest that serotonin release after 5-HTP administration leads to activation of motorneurons in the lumbar spinal cord. The mechanism of this activation may be related to the weakening of the inhibitory control of interneurons in the transmission pathways of the excitatory influences from muscle afferent to motorneurons and to the postural (antigravity) reflex reactions which necessary for the initiation of locomotion.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"41-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXPRESSION OF REG1Α, GAST AND TGFB1 GENES IN CONDITIONS OF STRESSINDUCED GASTRIC MUCOUS LESIONS DEVELOPMENT AND HEALING IN RATS. reg1Α、gast和tgfb1基因在应激诱导大鼠胃粘膜病变发生和愈合中的表达。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.01.053

A S Dranitsina, O O Morgaienko, D M Grebinyk, L I Ostapchenko

引用次数: 0

DOXORUBICIN-INDUSED DISTURBANCES OF CARDIOMYOCYTE CONTRACTILE ACTIVITY. 阿霉素引起的心肌细胞收缩活性紊乱。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.06.065

O Linnik, T Drevytska, K Tarasova, G Portnichenko, V Dosenko, I Mankovska

{"title":"DOXORUBICIN-INDUSED DISTURBANCES OF CARDIOMYOCYTE CONTRACTILE ACTIVITY.","authors":"O Linnik, T Drevytska, K Tarasova, G Portnichenko, V Dosenko, I Mankovska","doi":"10.15407/fz62.06.065","DOIUrl":"https://doi.org/10.15407/fz62.06.065","url":null,"abstract":"The aim of our study was to find out the mechanisms of cardiomyocyte contractile activity disturbances under the influence of anthracycline antibiotics and curcumin correction capabilities, to clarify the role of the mitochondrial membrane potential changes in cardiomyocyte contractile activity. It was investigated disturbances of contractile activity of the rat neonatal cardiomyocytes under the influence of anthracycline antibiotics (doxorubicin), and the possibility of correcting these violations using antioxidant curcumin. In addition, there was researched the role of mitochondrial membrane potential changes in reducing contractile activity of the cardiomyocytes. We established that doxorubicin causes significant changes in all reduction parameters compared to control: increased frequency of spontaneous contractions, violation of rhythm, lower amplitude and the shortening %, increase in the maximum speed of contraction and relaxation without changes in duration of these processes. Compatible incubation with doxorubicin and curcumin caused a consid- erable decrease of the incidence of spontaneous emission (3 times), restored their rhythm, increasing the amplitude of 2 times and the shortening % (8,15 ± 0,37), rising the maximum speed reduction to 1,8 times and the relaxation speed of 16 %, with no significant change in duration of these processes compared to monoincubation with doxorubicin. In the fluorescence study of neonatal cardiomyocytes mitochondrial potential was detected a significant reduction in mitochondria color brightness under the influence of doxorubicin - in 1,7 times compared to control. A compatible incubation of cells with curcumin and doxorubicin resulted in an elevation of mitochondria fluorescence (2,2 times compared to using of doxorubicin only).","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"65-71"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40436921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Disorders of carbohydrate metabolism in experimental brain injury. 实验性脑损伤中碳水化合物代谢紊乱。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.04.018

S V Ziablitsev, S V Pishchulina, S V Kolesnikova, R N Boris, Yuzkiv Ya S

引用次数: 3

EXCITABILITY PROPERTIES OF TRIGEMINAL GANGLION NEURONS. 三叉神经节神经元的兴奋性特性。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.024

M V Telka, O V Rikhalsky, N S Veselovsky

{"title":"EXCITABILITY PROPERTIES OF TRIGEMINAL GANGLION NEURONS.","authors":"M V Telka, O V Rikhalsky, N S Veselovsky","doi":"10.15407/fz62.02.024","DOIUrl":"https://doi.org/10.15407/fz62.02.024","url":null,"abstract":"The firing properties of small neurons (with diameters of soma less than 25 µm) were investigated using patch-clamp technique in whole-cell configuration in primary culture of trigeminal ganglia (TG) of postnatal rats. TG neurons were divided into three groups according to their firing responses to long-lasting depolarizing pulses: adaptive neurons (AN) characterized by a strongly adaptive responses; tonic neurons (TN) characterized by a multiple tonic firing; neurons with a delay before initiation of AP generation, namely, NDG. AN, TN and NDG also differed in AP electrophysiological and pharmacological characteristics. TN was distinguished by responses to hyperpolarization and the greatest value of input resistance. TN, AN and NDG were characterized by different active properties (amplitude of action potential and afterhyperpolarization, reobase, threshold). Each group of neurons was characterized by heterogeneity of AP duration and of frequency properties for TN. The application of tetrodotoxin (TTX) (250 nM) resulted in full or partial inhibition of AP generation and some neurons had TTX – insensitive firing responses. Neurons that were not affected by TTX had markedly longer AP. TTX had no effect on electrical activity of some AN and NDG. Based on sensitivity to TTX and their electrophysiological properties, AN and NDG seem to be C-fiber nococeptors.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"24-34"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Surface charge impact in nonsynaptic model of epilepsy in rat hippocampus. 大鼠海马非突触性癫痫模型的表面电荷影响。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.035

O S Zapukhliak, V O Kachanovska, E V Isaeva, O V Netsyk, D S Isaev

引用次数: 2

CHANGES IN ERYTHROCYTE SURFACE MARKER CD44 DURING HYPOTHERMIC AND LOW TEMPERATURE STORAGE. 低温和低温贮藏过程中红细胞表面标志物cd44的变化。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.094

N G Zemlianskykh, L A Babijchuk

{"title":"CHANGES IN ERYTHROCYTE SURFACE MARKER CD44 DURING HYPOTHERMIC AND LOW TEMPERATURE STORAGE.","authors":"N G Zemlianskykh, L A Babijchuk","doi":"10.15407/fz62.02.094","DOIUrl":"https://doi.org/10.15407/fz62.02.094","url":null,"abstract":"We studied the changes in surface marker CD44 in erythrocytes, cryopreserved under the protection of glycerol and PEG–1500, or stored in hypothermic conditions. It was shown that during hypothermic storage the CD44 characteristics in erythrocyte suspension were unchanged within 10 days. In cryopreserved erythrocytes a reduction in CD44–positive cells and in the level of expression of the surface marker were marked. Using PEG–1500 resulted in more pronounced change in erythrocyte CD44 characteristics after freeze–thawing in comparison with glycerol. Removal of cryoprotectants and the loss of a part of cells during the washing process led to the restoration of the CD44 characteristics in freeze–thawed erythrocytes suspension which successfully survived after the stresses. The results indicate that revealed changes in cryopreserved erythrocytes cover only a part of the cells, and they are associated with the instability of the population of erythrocytes with altered CD44 characteristics wherethrough after the removal of cryoprotectants with concomitant hemolysis of unstable cells the CD44 parameters in erythrocyte suspensions recovered. The mechanisms underlying the changes in the parameters of the surface marker CD44 in freeze–thawed erythrocyte may be related to the disruption of intermolecular interactions in the membrane under the influence of physical and chemical environmental factors, followed by the membrane vesiculation with the inclusion of the CD44 into the vesicles.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"94-102"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35911859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MITOCHONDRIAL DYSFUNCTION IN THE AGING HEART IS ACCOMPANIED BY CONSTITUTIVE NO-SYNTHASES UNCOUPLING ON THE BACKGROUND OF OXIDATIVE AND NITROSATIVE STRESS. 老化心脏中的线粒体功能障碍伴随着氧化和亚硝化应激背景下的组成性无合酶解偶联。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.003

N A Strutynska, A V Kotsiuruba, A Yu Budko, L A Mys, V F Sagach

{"title":"MITOCHONDRIAL DYSFUNCTION IN THE AGING HEART IS ACCOMPANIED BY CONSTITUTIVE NO-SYNTHASES UNCOUPLING ON THE BACKGROUND OF OXIDATIVE AND NITROSATIVE STRESS.","authors":"N A Strutynska, A V Kotsiuruba, A Yu Budko, L A Mys, V F Sagach","doi":"10.15407/fz62.02.003","DOIUrl":"https://doi.org/10.15407/fz62.02.003","url":null,"abstract":"We investigated the sensitivity of mitochondrial permeability transition pore (MPTP) opening to its inductor calcium, as well as indicators of oxidative and nitrosative stress in adult and old rat heart mitochondria and heart tissues. The coupling index of constitutive Ca2+/calmodulin-dependent NO-synthase (сNOS) was calculated based on experimentally found parameters. The aging is characterized by oxidative and nitrosative stress, which accompanied by a decrease of the cNOS coupling index and an increased sensitivity of MPTP to calcium. We found that in the heart mitochondria of old rats such oxidative stress indicators as the rate of generation of superoxide (• O2 - ) and hydroxyl (• OH) anion radicals were significantly increased (in 4 and 2,7 times respectively). Also, increased levels of urea and products of early lipid peroxidation – conjugated dienes display the intensification of oxidative stress. Such indicators of nitrosative stress as nitrate reductase and iNOS activity were also enhanced. At the same time under aging the NO2 - pools, generated when the heart oxygenation is normal, nitrosothiols pools and the activity of cNOS were decreased. It is due to the enhanced level of cNOS uncoupling, resulting in increased oxidative stress. It was also shown the lower level of hydrogen sulfide (H2S) in old rat heart mitochondria. Thus, we observed the increased sensitivity of MPTP to calcium, due to decreased concentration of its inhibitors nitric oxide and hydrogen sulfide and increased levels of its inducers ROS and RNS in old rat heart mitochondria.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35911970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

EVALUATION OF THE FUNCTIONAL STATE OF LIVER AND THE EFFICIENCY OF THERAPY FOR ENTEROPATHY OF CALVES. 犊牛肠病肝脏功能状态及治疗效果评价。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.06.102

V A Gryshchenko, T M Chernyshenko, O V Gornitska, T M Platonova

{"title":"EVALUATION OF THE FUNCTIONAL STATE OF LIVER AND THE EFFICIENCY OF THERAPY FOR ENTEROPATHY OF CALVES.","authors":"V A Gryshchenko, T M Chernyshenko, O V Gornitska, T M Platonova","doi":"10.15407/fz62.06.102","DOIUrl":"https://doi.org/10.15407/fz62.06.102","url":null,"abstract":"Comprehensive study of hepatospecific biochemical blood markers and haemostatic system in calves which recovered from gastrointestinal pathology at age 2-7 days, was con- ducted. Implementation of a complex of tests for evaluation of the liver's functional state and the efficiency of therapeutic regimens shown that the experimental animals compared to control at the 30th day of life demonstrated significantly increased conjugated bilirubin concentration (1.6 times), aspartate- and alanin aminotransferase activity (1.2 times), gamma-glutamyl transpeptidase (1.5 times) and alkaline phosphatase (1.4 times), and content of soluble fibrin monomer complexes (4 times). Such deviation from the norm of the mentioned parameters of liver's functional state suggests the need to monitor the liver state even 3 weeks after their clinical health is confirmed. To prevent the development of secondary hepatopathology it is recommended to conduct further medical correction of the functional state of the liver. It is found that to stimulate recovery of liver function in case of toxic dyspepsia in newborn calves, it is advisable to implement a phospholipid containing supplement <FLP-MDn as a measure ofreparative therapy till the animals are 30 days of life to ensure successful establishing of studied parameters.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"102-109"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40437875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

EFFECT OF HYDROGEN SULPHIDE DONOR ON INTRAOCULAR PRESSURE IN RATS. 硫化氢供体对大鼠眼压的影响。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01

L N Mikheytseva, T I Siroshtanenko

引用次数: 0