Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)最新文献_第4页

Role of matrix metalloproteinase 9 and its tissue inhibitor 1 in development and prognosis of diabetic retinopathy. 基质金属蛋白酶9及其组织抑制剂1在糖尿病视网膜病变发生及预后中的作用

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01

S V Ziablitsev, A V Korobova, O V Petrenko, V N Serduk, S U Mogilevsky

{"title":"Role of matrix metalloproteinase 9 and its tissue inhibitor 1 in development and prognosis of diabetic retinopathy.","authors":"S V Ziablitsev, A V Korobova, O V Petrenko, V N Serduk, S U Mogilevsky","doi":"","DOIUrl":"","url":null,"abstract":"The article presents the results of investigation of violations in the proteolysis system in patients with type 2 diabetes and diabetic retinopathy (DR). We studied the levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-I (TIMP-1) in the blood and in the intraocularfluid ofpatients with type 2 diabetes depending upon the stage of DR. We have established that the level of MMP-9 and TIMP- in the blood and in the intraocular fluid increased in patients with type 2 diabetes without signs of DR and with any stage of DR compared to the levels of these parameters in patients without diabetes. Increasing the levels of MMP-9 and TIMP-J took place with increasing of DR severity and reached its maximum levels at the proliferative stage of DR. It was found that the levels of MMP-9 in the intraocular fluid and type 2 diabetes duration affected theprobability of developing diabetic macular edema. At the level of MMP-9 105 ng/ml and 2 diabetes duration 10 years, the probability of developing macular edema was 100%. As a result of the construction ofpredictive models it was found that the level of MMP-9 in the intraocular fluid, stage of DR at the beginning of observation and type 2 diabetes duration had influence on the probability of the development ofproliferative DR over 4 years offollow-up. If the level of MMP-9 100 ng/ml, type 2 diabetes duration 10 years and absence of DR at the beginning of observation were observed the probability of developing proliferative DR would compose 85.9%.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 5","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36482593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[SUBUNIT SPECIFIC MODULATION OF GLYCINE RECEPTORS BY GINKGOLIC ACID.] 银杏酸对甘氨酸受体的亚基特异性调节。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.04.040

G Maleeva, S Buldakova, P Bregestovski

{"title":"[SUBUNIT SPECIFIC MODULATION OF GLYCINE RECEPTORS BY GINKGOLIC ACID.]","authors":"G Maleeva, S Buldakova, P Bregestovski","doi":"10.15407/fz62.04.040","DOIUrl":"https://doi.org/10.15407/fz62.04.040","url":null,"abstract":"Ginkgo biloba extract is a multicomponent pharmacological agent widely used in neurological disorders therapy. It was shown that ginkgolic acid, a constituent of lipophylic Ginkgo biloba extract, has numerous biological activities. In the present study we have focused on the features of ginkgolic acid action on αl and α2 glycine receptors that make part of the inhibitory system of the brain. Using whole-cell configuration of patch-clamp recording we analysed effects of ginkgolic acid on different subunits of glycine receptors. Experiments were performed on cultured Chinese hamster ovary cells (CHO cells), transfected with αl and α2 glycine receptor subunits. Ionic currents were induced by the fast application of different glycine concentrations. After 20-40 sec of pre-treatment with ginkgolic acid (25μM) currents mediated by al glycine receptors reversibly increased from 364±49 pA, (n=34) to 846±134 pA, (n=34). EC(50) for glycine has changed from 36±6 μM (control) to 17±2 μM. In contrast, the application of ginkgolic acid on glycine receptors formed by α2 subunits did not provoke potentiation. Our results demonstrate that ginkgolic acid is a subunit specific modulator of glycine receptors. The mechanisms of the ginkgolic acid action on glycine receptors require further investigation.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"40-45"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MORPHOLOGICAL CHANGES IN MICE LIVER IN DYNAMICS OF CONCANAVALIN A - INDUCED HEPATITIS. 豆豆蛋白a致肝炎小鼠肝脏形态学变化。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.01.083

S I Pavlovych, N V Makogon, N G Grushka, T M Bryzgina, R I Janchiy

{"title":"MORPHOLOGICAL CHANGES IN MICE LIVER IN DYNAMICS OF CONCANAVALIN A - INDUCED HEPATITIS.","authors":"S I Pavlovych, N V Makogon, N G Grushka, T M Bryzgina, R I Janchiy","doi":"10.15407/fz62.01.083","DOIUrl":"https://doi.org/10.15407/fz62.01.083","url":null,"abstract":"The injure of the liver tissue and its infiltration by cells of the innate and adaptive immunity in dynamics of Con A-induced hepatitis in mice was studied. The semiquantitative method of damage rate of microcirculation channel and liver parenchyma was used, leukocyte liver infiltration and cellular composition of infiltrates were investigated also. Primary liver reaction to the Con-A was the inflammatory changes in the vascular bed, followed by disturbances in the parenchyma.The sufficient increasing of leukocyte migration to the liver was revealed. Besides, the neutrophile infiltration was increased first with a maximum at 6 hours of the experiment (63,9 ±4,6%, p<0,001 to the control level) ,and then the lymphocyte infiltration was increased with creation of manycellular lymphocytemacrophage infiltrates (62% at 48 hours comparing to 6 hours of experiment) and sufficient quantity of plasma cells population (4,9%, p<0,05 comparing to 6 hours of experiment). The obtained data gives the base to suggest that the elevated infiltration of liver tissue by leukocytes, particularly by lymphocytes and monocytes, together with necrotic death increasing creats the conditions for effective intracellular interaction and immune response to autoantigenes. This can be the essential pathogenic mechanism of development of autoimmune liver deseases.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 1","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SERUM LEVELS OF ENDOTHELIAL MONOCYTE ACTIVATING PEPTIDE II IN TYPE 1 DIABETES. 1型糖尿病患者血清内皮单核细胞活化肽ii的水平。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.01.102

L A Mogylnytska, O E Mogylnytska

引用次数: 0

Glucose tolerance in obese men is associated with dysregulation of some angiogenesis-related gene expressions in subcutaneous adipose tissue. 肥胖男性的糖耐量与皮下脂肪组织中一些血管生成相关基因表达的失调有关。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.02.012

O H Minchenko, Y M Bashta, D O Minchenko, O O Ratushna

{"title":"Glucose tolerance in obese men is associated with dysregulation of some angiogenesis-related gene expressions in subcutaneous adipose tissue.","authors":"O H Minchenko, Y M Bashta, D O Minchenko, O O Ratushna","doi":"10.15407/fz62.02.012","DOIUrl":"https://doi.org/10.15407/fz62.02.012","url":null,"abstract":"Obesity and its metabolic complications are one of the most profound public health problems and result from interactions between genes and environmental. The development of obesity is tightly connected with dysregulation of intrinsic gene expression mechanisms controlling majority of metabolic processes, which are essential for regulation many physiological functions, including insulin sensitivity, cellular proliferation and angiogenesis. Our objective was to evaluate if expression of angiogenesis related genes VEGF-A, CYR61, PDGFC, FGF1, FGF2, FGFR2, FGFRL1, E2F8, BAI2, HIF1A, and EPAS1 at mRNA level in adipose tissue could participate in the development of obesity and metabolic complications. We have shown that expression level of VEGF-A, PDGFC, FGF2, and FGFRL1 genes is decreased in adipose tissue of obese men with normal glucose tolerance (NGT) versus a group of control subjects. At the same time, in this group of obese individuals a significant up-regulation of CYR61, FGF1, FGFR2, E2F8, BAI2, and HIF1A gene expressions was observed. Impaired glucose tolerance (IGT) in obese patients associates with down-regulation of CYR61 and FGFR2 mRNA and up-regulations of E2F8, FGF1, FGF2, VEGF-A and its splice variant 189 mRNA expressions in adipose tissue versus obese (NGT) individuals. Thus, our data demonstrate that the expression of almost all studied genes is affected in subcutaneous adipose tissue of obese individuals with NGT and that glucose intolerance is associated with gene-specific changes in the expression of E2F8, FGF1, FGF2, VEGF-A, CYR61 and FGFR2 mRNAs. The data presented here provides evidence that VEGF-A, CYR61, PDGFC, FGF1, FGF2, FGFR2, FGFRL1, E2F8, BAI2, and HIF1A genes are possibly involved in the development of obesity and its complications.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"12-23"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35911974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[EFFECT OF L-ARGININE - NO ON PROOXIDANT-ANTIOXIDANT BALANCE IN ERYTHROCYTES OF RATS UNDER ALCOHOL INTOXICATION.] l -精氨酸- no对酒精中毒大鼠红细胞促氧化-抗氧化平衡的影响

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.04.076

N V Yefimenko, N O Sybirna

引用次数: 0

STATUS OF GLUTATHIONE-DEPENDENT LINK OF BLOOD ANTIOXIDANT SYSTEM UNDER APPLICATION OF FENUGREEK SEED POWDER IN ANIMALS WITH EXPERIMENTAL OBESITY. 胡芦巴籽粉对实验性肥胖动物血液抗氧化系统谷胱甘肽依赖环节的影响。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01

V V Konopelnyuk, L I Kot, T V Koval, T D Khilko, I V Yakubtsova, T D Preobrazhenska, L I Ostapchenko

引用次数: 0

BONE REGULATORY MECHANISMS DESTRUCTION IN EXPERIMENTAL CHRONIC KIDNEY DISEASE. 实验性慢性肾脏疾病的骨调节机制破坏。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.03.054

S B Pavlov, M V Kumechko, O B Litvinova, N M Babenko, A V Goncharova

{"title":"BONE REGULATORY MECHANISMS DESTRUCTION IN EXPERIMENTAL CHRONIC KIDNEY DISEASE.","authors":"S B Pavlov, M V Kumechko, O B Litvinova, N M Babenko, A V Goncharova","doi":"10.15407/fz62.03.054","DOIUrl":"https://doi.org/10.15407/fz62.03.054","url":null,"abstract":"The aim of our study was to investigate the role of intercellular\u0000mediators – interleukin-1 receptor antagonist (IL-1 RA),\u0000interleukin-17 (IL-17), receptor activator of nuclear factor kB\u0000ligand (RANKL) and osteoprotegerin in the mechanisms of\u0000metabolic regulation of renal and bone tissue on model of violations\u0000of bone remodeling in chronic kidney disease (CKD).\u0000It was found a significant increase in the content of cytokines\u0000IL-1 RA (4,207 ± 0,546 pg/ml), IL-17 (33,944 ± 0,938 pg/\u0000ml), osteoprotegerin (28,338 ± 1,223 pg/ml) and RANKL\u0000(0,184 ± 0,018 pmol/l) in the serum of animals in violation\u0000of bone remodeling in CKD compared with the contents of\u0000the studied cytokines in animals in the control group (2,529\u0000± 0,132 pg/ml, 28,166 ± 0,526 pg/ml, 21,588 ± 0,763 pg/ml\u0000and 0,131 ± 0,006 pmol/l, respectively) (P<0.05). The obtained\u0000correlations reflect the relationship between regulation of bone\u0000remodeling and the development of inflammation in CKD.\u0000The imbalance between pro- and anti-inflammatory cytokine\u0000plays an important role both in the development of CKD and\u0000in processes of bone remodeling.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 3","pages":"54-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Modulation of the hippocampal propensity to non- synaptic epileptiform synchronization in low-calcium model of epilepsy. 低钙癫痫模型海马非突触性癫痫样同步化倾向的调节。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01

O S Zapukhliak, O V Netsyk, O S Rasulova, D S Isaev

{"title":"Modulation of the hippocampal propensity to non- synaptic epileptiform synchronization in low-calcium model of epilepsy.","authors":"O S Zapukhliak, O V Netsyk, O S Rasulova, D S Isaev","doi":"","DOIUrl":"","url":null,"abstract":"The CA3 and CAI regions are the main stages of the \"three-synaptic pathway\", which plays a role in the generation of hyper-synchronous events in the hippocampus. Under certain experimental conditions, this brain structure might support pathological epileptiform synchronization that is independent of active chemical synaptic transmission. In present work, we estimated the conditions that would facilitate non- synaptic synchronization of the hippocampus. Non-synaptic epileptiform activity was induced in hippocampal slices by the omission calcium ions from the extracellular milieu. The propensity of hippocampal regions to nonsynaptic interactions was estimated by measuring the delay time neededfor the development of low-Ca²⁺ discharges in the CA3 and CAI. Next, an increase of neuronal excitability was induced by the pre- incubation ofhippocampal slices in 4-aminopyridine (4-AP) and by the reduction ofextracellular osmolarity. Pre-incubation of hippbcampal slices with 4-AP under normal osmotic conditions resulted in decreased latency for non-synaptic discharges in the CA3, but not in the CAl. However hypo-osmotic conditions caused increased excitability of the CA3 region, which resulted in decreased delay time for nonsynaptic discharges and this level of cellular excitability was not further enhanced by the pre-incubation with 4-AR.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40437376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MORPHOLOGICAL CHANGES PANCREAS AFTER OF MELATONIN'S EFFECT IN DIFFERENT SEASONS. 褪黑素在不同季节作用后胰腺的形态变化。

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Pub Date : 2016-01-01 DOI: 10.15407/fz62.06.088

R V Yanko

{"title":"MORPHOLOGICAL CHANGES PANCREAS AFTER OF MELATONIN'S EFFECT IN DIFFERENT SEASONS.","authors":"R V Yanko","doi":"10.15407/fz62.06.088","DOIUrl":"https://doi.org/10.15407/fz62.06.088","url":null,"abstract":"Morphological changes of the pancreas of young rats after exposure of exogenous melatonin in the spring and autumn periods was investigated. Exogenous melatonin (Unipharm Inc., USA) was administered to experimental group of animals daily'at a dose 5 mg/kg. The duration of the experiment was 28 days. It was revealed that the exocrine part of the.pancreas responds differently to the effects of melatonin at different times of the year. Thus, after administration of melatonin in the spring increase of the size of acinus, the height of the epithelium (by 7 %), area exocrinocytes (by 58 %), of their nucleus (by 20 %) and cytoplasm (69 %), the amount of nucleoli in cells (18 %), reduction the amount of connective tissue elements. Melatonin introduction in the autumn decrease in the size of acinus, height and area of exocrinocytes, growth the number of exocrinocytes in the acinus, nucleoli and width layers interlobular connective tissue in the gland. This may indicate that melatonin increases in the spring of the synthetic activity of the exocrine pancreas, whereas in the autumn (for the majority of the morphometric parameters) - somewhat reduces its functional state. The administration of melatonin in the spring (mostly) and in the autumn periods increased the functional activity of the endocrine pancreas. This is indicated by growth in the size of Langerhans islets, increasing the number and density of the (autumn) endocrinocytes.","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40437378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3