EXCITABILITY PROPERTIES OF TRIGEMINAL GANGLION NEURONS.

Abstract

The firing properties of small neurons (with diameters of soma less than 25 µm) were investigated using patch-clamp technique in whole-cell configuration in primary culture of trigeminal ganglia (TG) of postnatal rats. TG neurons were divided into three groups according to their firing responses to long-lasting depolarizing pulses: adaptive neurons (AN) characterized by a strongly adaptive responses; tonic neurons (TN) characterized by a multiple tonic firing; neurons with a delay before initiation of AP generation, namely, NDG. AN, TN and NDG also differed in AP electrophysiological and pharmacological characteristics. TN was distinguished by responses to hyperpolarization and the greatest value of input resistance. TN, AN and NDG were characterized by different active properties (amplitude of action potential and afterhyperpolarization, reobase, threshold). Each group of neurons was characterized by heterogeneity of AP duration and of frequency properties for TN. The application of tetrodotoxin (TTX) (250 nM) resulted in full or partial inhibition of AP generation and some neurons had TTX – insensitive firing responses. Neurons that were not affected by TTX had markedly longer AP. TTX had no effect on electrical activity of some AN and NDG. Based on sensitivity to TTX and their electrophysiological properties, AN and NDG seem to be C-fiber nococeptors.