Exploration (Beijing, China)最新文献_第5页

Facile construction of dual-response super-resolution probes for tracking organelles dynamics 轻松构建用于跟踪细胞器动态的双响应超分辨率探针

Exploration (Beijing, China) Pub Date : 2024-03-12 DOI: 10.1002/EXP.20230145

Daili Liu, Guiqian Fang, Yanfeng Wang, Caicai Meng, Zhidong Liu, Qixin Chen, Xintian Shao

{"title":"Facile construction of dual-response super-resolution probes for tracking organelles dynamics","authors":"Daili Liu, Guiqian Fang, Yanfeng Wang, Caicai Meng, Zhidong Liu, Qixin Chen, Xintian Shao","doi":"10.1002/EXP.20230145","DOIUrl":"10.1002/EXP.20230145","url":null,"abstract":"Super-resolution imaging techniques, such as structured illumination microscopy (SIM), have enabled researchers to obtain nanoscale organelle-level outputs in living systems, but they impose additional stringent requirements on fluorescence probes. However, high-performance, custom-designed SIM probes that can explain underlying biological processes remain unavailable. Herein, a customizable engineering toolkit is developed for the facile assembly of SIM probes suitable for subcellular component detection. This toolkit is used to customize a fluorescent molecule, CPC (coumarin–phenylhydrazine–carboxyl), capable of simultaneously monitoring peroxynitrite (ONOO−) and polarity distribution in mitochondria and lipid droplets (LDs), respectively, through functional ON–OFF mechanisms. The customized CPC molecule demonstrated excellent imaging capabilities under SIM, enabled the successful localization of multiple organelles, and reliably tracked the distribution of different components, thus facilitating the study of the interplay between organelles. Using CPC, the physical transition of intracellular LDs is demonstrated from heterogeneity to homogeneity. This was specifically observed during ferroptosis where the polarity of the LDs increased and their morphology became more contracted. Furthermore, the loss of LDs functionality could not counteract the accumulation of ONOO− within the mitochondria, leading to the decoupling of mitochondrial LDs during ferroptosis. These results confirmed the potential mechanism of LDs dysfunction and decoupling triggered via cumulative mitochondrial oxidative stress during ferroptosis. To summarize, this toolkit will be a powerful tool for examining subtle variations among components during the interplay between different organelles, thus offering novel avenues for understanding and treating related diseases.","PeriodicalId":72997,"journal":{"name":"Exploration (Beijing, China)","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/EXP.20230145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140251247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-assembled, genetically engineered, irradiated tumor cell debris vaccine 自组装的基因工程辐照肿瘤细胞碎片疫苗

Exploration (Beijing, China) Pub Date : 2024-03-06 DOI: 10.1002/EXP.20220170

Yajie Sun, Yan Hu, Yuanyuan Geng, Chao Wan, Yang Liu, Yifei Liao, Xiujuan Shi, Jonathan F. Lovell, Kunyu Yang, Honglin Jin

{"title":"A self-assembled, genetically engineered, irradiated tumor cell debris vaccine","authors":"Yajie Sun, Yan Hu, Yuanyuan Geng, Chao Wan, Yang Liu, Yifei Liao, Xiujuan Shi, Jonathan F. Lovell, Kunyu Yang, Honglin Jin","doi":"10.1002/EXP.20220170","DOIUrl":"10.1002/EXP.20220170","url":null,"abstract":"Vaccine-based therapeutics for cancers face several challenges including lack of immunogenicity and tumor escape pathways for single antigen targets. It has been reported that radiotherapy has an in situ vaccine effect that provides tumor antigens following irradiation, helping to activate antigen-presenting cells (APCs). Herein, a new vaccine approach is developed by combining genetically engineered irradiated tumor cell debris (RTD) and hyaluronic acid (HA), termed HA@RTD. A cancer cell line is developed that overexpresses granulocyte-macrophage colony-stimulating factor (GM-CSF). A hydrogel was developed by covalent conjugation of HA with RTD proteins that acted as a potent vaccine system, the effects which were probed with T cell receptor sequencing. The engineered vaccine activated antitumor immunity responses and prevented tumor growth in mice even with a single immunization. HA@RTD vaccine efficacy was also assessed in therapeutic settings with established tumors and in combination with immune checkpoint blockade.","PeriodicalId":72997,"journal":{"name":"Exploration (Beijing, China)","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/EXP.20220170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140078530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in mitochondria-targeting theranostic agents 线粒体靶向治疗药物的最新进展

Exploration (Beijing, China) Pub Date : 2024-03-05 DOI: 10.1002/EXP.20230063

Kun Qian, Shu Gao, Zhaoning Jiang, Qihang Ding, Zhen Cheng

引用次数: 0

Recent advances in bacteria-based platforms for inflammatory bowel diseases treatment 基于细菌的炎症性肠病治疗平台的最新进展

Exploration (Beijing, China) Pub Date : 2024-03-05 DOI: 10.1002/EXP.20230142

Jiaoying Lu, Xinyuan Shen, Hongjun Li, Juan Du

引用次数: 0

Impact of diabetes mellitus on tuberculosis prevention, diagnosis, and treatment from an immunologic perspective 从免疫学角度看糖尿病对结核病预防、诊断和治疗的影响

Exploration (Beijing, China) Pub Date : 2024-03-05 DOI: 10.1002/EXP.20230138

Zhaoyang Ye, Linsheng Li, Ling Yang, Li Zhuang, Ashok Aspatwar, Liang Wang, Wenping Gong

{"title":"Impact of diabetes mellitus on tuberculosis prevention, diagnosis, and treatment from an immunologic perspective","authors":"Zhaoyang Ye, Linsheng Li, Ling Yang, Li Zhuang, Ashok Aspatwar, Liang Wang, Wenping Gong","doi":"10.1002/EXP.20230138","DOIUrl":"10.1002/EXP.20230138","url":null,"abstract":"The coexistence of diabetes mellitus (DM) and tuberculosis (TB) presents a significant global burden, with DM being recognized as a major risk factor for TB. This review comprehensively analyzes the immunological aspects of DM-TB comorbidity, shedding light on the impact of DM on TB pathogenesis and immune responses. It reveals that high blood glucose levels in TB patients contribute to reduced innate immune cell count, compromised phagocytic function, and delayed antigen presentation. These factors ultimately impair the clearance of Mycobacterium tuberculosis (MTB) and delay adaptive immune responses. With the interaction between TB and DM, there is an increase in inflammation and elevated secretion of pro-inflammatory cytokines by immune cells. This exacerbates the inflammatory response and contributes to poor treatment outcomes in TB. Moreover, the review explores the effects of DM on TB prevention, diagnosis, and treatment. It highlights how poor glycemic control, insulin resistance (IR), DM complications, and genetic factors increase the risk of MTB infection in individuals with DM. Additionally, DM-related immune suppression adversely affects the sensitivity of traditional diagnostic tests for TB, potentially resulting in underdiagnosis and delayed intervention. To mitigate the burden of TB in DM patients, the review emphasizes the need for further research on the mechanisms underlying DM reactivation in latent TB infection (LTBI). It shows how important it is to find and treat LTBI in DM patients as soon as possible and suggests looking into biomarkers that are specific to DM to make diagnosis more accurate.","PeriodicalId":72997,"journal":{"name":"Exploration (Beijing, China)","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/EXP.20230138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140265295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress and application of high efficiency organic solar cells based on benzodithiophene donor materials 基于苯并二噻吩给体材料的高效有机太阳能电池的研究进展与应用

Exploration (Beijing, China) Pub Date : 2024-02-29 DOI: 10.1002/EXP.20230122

Congqi Lin, Ruixiang Peng, Jingyu Shi, Ziyi Ge

引用次数: 0

Bioprinted research models of urological malignancy 泌尿系统恶性肿瘤生物打印研究模型

Exploration (Beijing, China) Pub Date : 2024-02-20 DOI: 10.1002/EXP.20230126

Guanyi Wang, Xiongmin Mao, Wang Wang, Xiaolong Wang, Sheng Li, Zijian Wang

{"title":"Bioprinted research models of urological malignancy","authors":"Guanyi Wang, Xiongmin Mao, Wang Wang, Xiaolong Wang, Sheng Li, Zijian Wang","doi":"10.1002/EXP.20230126","DOIUrl":"10.1002/EXP.20230126","url":null,"abstract":"Urological malignancy (UM) is among the leading threats to health care worldwide. Recent years have seen much investment in fundamental UM research, including mechanistic investigation, early diagnosis, immunotherapy, and nanomedicine. However, the results are not fully satisfactory. Bioprinted research models (BRMs) with programmed spatial structures and functions can serve as powerful research tools and are likely to disrupt traditional UM research paradigms. Herein, a comprehensive review of BRMs of UM is presented. It begins with a brief introduction and comparison of existing UM research models, emphasizing the advantages of BRMs, such as modeling real tissues and organs. Six kinds of mainstream bioprinting techniques used to fabricate such BRMs are summarized with examples. Thereafter, research advances in the applications of UM BRMs, such as culturing tumor spheroids and organoids, modeling cancer metastasis, mimicking the tumor microenvironment, constructing organ chips for drug screening, and isolating circulating tumor cells, are comprehensively discussed. At the end of this review, current challenges and future development directions of BRMs and UM are highlighted from the perspective of interdisciplinary science.","PeriodicalId":72997,"journal":{"name":"Exploration (Beijing, China)","volume":"4 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/EXP.20230126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140445316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen-carrying semiconducting polymer nanoprodrugs induce sono-pyroptosis for deep-tissue tumor treatment 载氧半导体聚合物纳米药物诱导声突变，用于深层组织肿瘤治疗

Exploration (Beijing, China) Pub Date : 2024-02-19 DOI: 10.1002/EXP.20230100

Fengshuo Wang, Yongliang Fan, Yue Liu, Xiangxin Lou, Linawati Sutrisno, Shaojun Peng, Jingchao Li

{"title":"Oxygen-carrying semiconducting polymer nanoprodrugs induce sono-pyroptosis for deep-tissue tumor treatment","authors":"Fengshuo Wang, Yongliang Fan, Yue Liu, Xiangxin Lou, Linawati Sutrisno, Shaojun Peng, Jingchao Li","doi":"10.1002/EXP.20230100","DOIUrl":"10.1002/EXP.20230100","url":null,"abstract":"Sonodynamic therapy (SDT) has been explored for cancer therapy, especially for deep tumors due to its low tissue penetration restriction. The therapeutic efficacy of SDT is limited due to the complicated tumor microenvironment. This study reports the construction of oxygen-carrying semiconducting polymer nanoprodrugs (OSPNpro) for deep tumor treatment via combining amplified SDT with pyroptosis. An oxygen carrier perfluorohexane, sonodynamic semiconducting polymer as the sonosensitizer, and reactive oxygen species (ROS)-responsive prodrug are co-loaded into a nanoparticle system, leading to the formation of these polymer nanoprodrugs. Such OSPNpro show an effective accumulation in tumor tissues after systemic administration, in which they deliver oxygen to relieve tumor hypoxia microenvironment and thus mediate amplified SDT via producing ROS under ultrasound (US) irradiation, even when the tumors are covered with a 2-cm chicken breast tissue. In addition, the ROS-responsive prodrugs are activated by the generated ROS to trigger pyroptosis of tumor cells. Such a sono-pyroptosis induces a strong antitumor immunity with obviously higher level infiltrations of effector immune cells into tumors. Therefore, OSPNpro-based combinational therapy can greatly inhibit the growth of 2-cm chicken breast tissue-covered deep tumors and suppress tumor metastasis. This study offers a prodrug nanoplatform for treatment of deep tumor via sono-pyroptosis strategy.","PeriodicalId":72997,"journal":{"name":"Exploration (Beijing, China)","volume":"4 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/EXP.20230100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139958488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frontispiece: Viscoelastic blood coagulation testing system enabled by a non-contact triboelectric angle sensor (EXP2 1/2024) 正面图：由非接触式三电角度传感器支持的粘弹性血液凝固测试系统（EXP2 1/2024）

Exploration (Beijing, China) Pub Date : 2024-02-14 DOI: 10.1002/EXP.20240105

Baocheng Wang, Xuelian Wei, Hanlin Zhou, Xiaole Cao, Enyang Zhang, Zhong Lin Wang, Zhiyi Wu

引用次数: 0

Inside Front Cover: Recent advances in battery characterization using in situ XAFS, SAXS, XRD, and their combining techniques: From single scale to multiscale structure detection (EXP2 1/2024) 封面内页：使用原位 XAFS、SAXS、XRD 及其组合技术进行电池表征的最新进展：从单一尺度到多尺度结构检测（EXP2 1/2024）

Exploration (Beijing, China) Pub Date : 2024-02-14 DOI: 10.1002/EXP.20240102

Weidong Cheng, Mengyuan Zhao, Yuecheng Lai, Xin Wang, Huanyan Liu, Peng Xiao, Guang Mo, Bin Liu, Yunpeng Liu

引用次数: 0