Engineered regeneration最新文献

{"title":"Flexible wearable sensors: An emerging platform for monitoring of bacterial infection in skin wounds","authors":"Hao Meng ,&nbsp;Weicheng Zhong ,&nbsp;Kui Ma ,&nbsp;Jianlong Su ,&nbsp;Liqian Ma ,&nbsp;Yaying Hao ,&nbsp;Yufeng Jiang ,&nbsp;Xi Liu ,&nbsp;Xiaobing Fu ,&nbsp;Cuiping Zhang","doi":"10.1016/j.engreg.2024.03.003","DOIUrl":"10.1016/j.engreg.2024.03.003","url":null,"abstract":"<div><p>Persistent inflammatory responses often occur when bacteria and other microorganisms frequently invade and colonize open wounds and eventually result in the formation of chronic wounds. Therefore, achieving real-time detection of invasive bacteria accurately and promptly is essential for efficient wound management and accelerating the healing process. Recently, flexible wearable sensors have garnered significant attention, especially those designed for monitoring real-time biophysical or biochemical signals in wound sites in a minimally invasive manner. They provide more precise and continuous monitoring data, making them as emerging tools for clinical diagnostics. In this review, we first discuss the species and community distribution of different types of bacteria in chronic wounds. Next, we introduce currently developed techniques for detecting bacteria at wound sites. Following that, we discuss the recent progress and unresolved issues of various flexible wearable sensors in detecting bacteria at wound sites. We believe that this review can provide meaningful guidance for the development of flexible wearable sensors for bacteria detection.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 186-198"},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000173/pdfft?md5=2241e9cc26637373093e4812a2c1d06d&pid=1-s2.0-S2666138124000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140269284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma","authors":"Feng Liang ,&nbsp;Xueying An ,&nbsp;Ruoxi Wang ,&nbsp;Wenshu Wu ,&nbsp;Lin Yang ,&nbsp;Yixin Zheng ,&nbsp;Qing Jiang ,&nbsp;Xingquan Xu ,&nbsp;Danni Zhong ,&nbsp;Min Zhou","doi":"10.1016/j.engreg.2024.03.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.03.002","url":null,"abstract":"<div><p>Osteosarcoma (OS) is one of the most common malignant tumors in children and young adults. As chemotherapy and other therapies are limited by low therapeutic efficiency, severe side effects and single therapeutic function, it is of high value to develop innovative therapies for precise and efficient treatment of OS. Herein, natural photosynthetic microalgae (<em>C. vulgaris,</em> CV) were utilized as carriers for the chemotherapeutic agent doxorubicin (DOX) to create a multifunctional therapeutic platform (CV@DOX) for the photo-modulation of the tumor microenvironment (TME) and synergistic chemo-photodynamic therapy of osteosarcoma. CV@DOX exhibited rapid drug release behavior in the acidic TME, improving the efficiency of chemotherapy against tumors and reducing side effects on other normal tissues. Under 650 nm laser irradiation, CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species (ROS), thereby enhancing photodynamic therapy (PDT). CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis, inhibiting tumor proliferation and angiogenesis. Moreover, chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after administration. Finally, CV@DOX did not cause significant hematological and tissue toxicity, and prevented DOX-induced cardiotoxicity, showing good <em>in vivo</em> biocompatibility. Overall, this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosarcoma treatment.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 199-209"},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000161/pdfft?md5=1c51e142d1f961dce12e5ebd7ecf0d4d&pid=1-s2.0-S2666138124000161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140534985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging microfluidics for the modeling and treatment of arthritis","authors":"Nengjie Yang ,&nbsp;Chi Sun ,&nbsp;Chen Dong ,&nbsp;Yuting Huang ,&nbsp;Yujuan Zhu ,&nbsp;Zhifeng Gu","doi":"10.1016/j.engreg.2024.02.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.02.002","url":null,"abstract":"<div><p>Microfluidic is a technology that allows the precise control of fluid in a micro-channel. With its advantages of high throughput and low cost, microfluidic technology has achieved good performance in various fields in recent years. Arthritis is a general term for a variety of joint diseases, which can be clinically manifested as joint pain and swelling, seriously affecting people's physical and mental health. At present, the causes of arthritis disease are still unknown, and existing disease models and treatment methods are still limited, so more treatments need to be developed. Microfluidic organ chip is a cutting-edge technology to build a bionic human organ model, which can reflect the structure and function characteristics of human organs by simulating the physiological environment of tissues and cells in vitro. This paper reviews the application of microfluidic technology in the modeling and treatment of arthritis, hoping to open up a new vision for the study of arthritis.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 153-169"},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000124/pdfft?md5=6414d4f1e22121b9b306c0f401d788ff&pid=1-s2.0-S2666138124000124-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140052530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Piezo1 and Piezo2 proteins in tissue engineering: A Comprehensive review","authors":"Tejaswini Tadge ,&nbsp;Ashwini Pattewar ,&nbsp;Namdev More ,&nbsp;Srivalliputtur Sarath Babu ,&nbsp;Ravichandiran Velyutham ,&nbsp;Govinda Kapusetti","doi":"10.1016/j.engreg.2024.03.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.03.001","url":null,"abstract":"<div><p>Almost every life form, from the tiniest bacterium to humans, is mechanosensitive, implying it can use mechanical stresses to trigger certain physiological responses in the form of electric signals. Mechanotransduction largely relies on ion channels that respond to mechanical forces, such as the epithelial sodium channels/degenerins, transient receptor potential channel, and the two-pore domain potassium channel. Piezo1 and Piezo2 proteins were discovered to be the biggest non-selective mechanosensitive cation channels in the cell membrane. A substantial amount of research has previously been published on the Piezo channel's function in touch sensation, balance, and cardiovascular regression. However, the mechanistic perspective must be refined to fully understand the role of Piezo proteins in tissue engineering. This review centers on the latest insights into the structure of Piezo channels, activation mechanisms, and its interactions with cytoskeletal components, by emphasizing the physiological activities of Piezo channels in different tissues. The study also places focus on the possibilities of targeting this cation channel family as a tissue regeneration aid.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 170-185"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266613812400015X/pdfft?md5=5badeb709871ca7eeeff6029118218a2&pid=1-s2.0-S266613812400015X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering neuroregenerative microenvironment via aligned hydrogel-assisted magnetic stimulation for complete spinal cord injury repair","authors":"Chun-Yi Yang ,&nbsp;Zhe Meng ,&nbsp;Zhijun He ,&nbsp;Pengchao Ma ,&nbsp;Zhaohui Hou ,&nbsp;Kunkoo Kim ,&nbsp;Jingsong Lu ,&nbsp;Kaiyuan Yang ,&nbsp;Guihuai Wang ,&nbsp;Xiumei Wang","doi":"10.1016/j.engreg.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.02.001","url":null,"abstract":"<div><p>Utilizing biomaterials in tissue engineering has shown considerable promise for tissue regeneration, particularly through delivering multimodel cell-regulatory signals, including the material-related signals and extrinsic stimuli. In this research, we developed a magnetic-responsive aligned nanofiber fibrin hydrogel (MAFG), integrating the structured alignment of nanofibers and the pliability of fibrin hydrogel with an external magnetic field. This design aimed to enhance the regenerative response in spinal cord injury treatment. A medium-strength magnetic field, aligned with the spinal cord, was applied to aid motor function recovery in rats with spinal cord injuries. The use of MAFG in this context not only intensified the effect of the magnetic field but also encouraged the activation and differentiation of native neural stem cells. Furthermore, this method effectively steered macrophage polarization towards a beneficial M2 phenotype, addressing immune dysregulation at the injury site. The parallel application of magnetic field stimulation through MAFG in a spinal cord injury model contributed to the concurrent promotion of neurogenesis, angiogenesis, and immunomodulation, resulting in marked improvement in motor function in rats. This investigation underscores the therapeutic potential of magnetic field stimulation and highlights how aligning this stimulation with the spinal cord can significantly enhance the regenerative milieu at the injury site.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 139-152"},"PeriodicalIF":0.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000112/pdfft?md5=df0eb571f273092c0489ff3db3501fbb&pid=1-s2.0-S2666138124000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding updating Declaration of Competing Interest statements in previously published articles","authors":" ","doi":"10.1016/j.engreg.2024.02.003","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.02.003","url":null,"abstract":"","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"1525 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139830997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of NFATc1 through NF-kB/PI3K signaling pathway by Oleandrin to inhibit osteoclastogenesis and bone resorption","authors":"Zhikun Li ,&nbsp;Kai Chen ,&nbsp;Qifeng Yu ,&nbsp;Yifan Li ,&nbsp;Shichao Tong ,&nbsp;Ruijun Xu ,&nbsp;Ruixi Hu ,&nbsp;Yi Zhang ,&nbsp;Wei Xu","doi":"10.1016/j.engreg.2024.01.005","DOIUrl":"10.1016/j.engreg.2024.01.005","url":null,"abstract":"<div><p>Inflammation can initiate osteolysis, which is the breakdown of bone by fully developed osteoclasts. The compound Oleandrin is recognized for its effects against inflammation and tumors. Our objective was to examine the effects of Oleandrin on osteoclastogenesis and osteolysis, both in vitro and in vivo. In vitro, the impact of Oleandrin on osteoclastogenesis was assessed using CCK-8 assays, TRAP staining, and bone resorption assays. Additionally, a mouse model of osteolysis caused by LPS injection into the calvaria was used to conduct an in vivo investigation, examining bone histomorphology, histology, and immunohistochemistry. In vitro, concentrations of 5 nM and 10 nM of Oleandrin were found to be non-cytotoxic based on the results obtained. In vitro, Oleandrin hindered the osteoclastogenesis and bone resorption induced by RANKL. Oleandrin successfully inhibited the phosphorylation of NF-κB p65 and PI3K p85 in osteolytic tissue, thereby suppressing LPS-induced inflammatory osteolysis in mice calvaria during the in vivo study. Furthermore, the Oleandrin-treated group exhibited a noteworthy decrease in the expression level of NFATc1, which is a crucial controller of osteoclastogenesis. To sum up, our discoveries indicate that Oleandrin could hinder osteoclastogenesis and bone resorption, thereby having the ability to suppress inflammation-induced osteolysis. The underlying mechanism involves the NF-κB/PI3K pathway and inhibition of NFATc1 activation. Therefore, the findings suggest that Oleandrin holds potential as a therapeutic remedy for osteolytic ailments.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 342-349"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000057/pdfft?md5=78bcdfed64502d036c962f4dfed847e1&pid=1-s2.0-S2666138124000057-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139686754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Muscle Tendon Junction in vitro Using Aligned Electrospun PCL Fibres","authors":"Nodoka Iwasaki ,&nbsp;Marta Roldo ,&nbsp;Aikaterina Karali ,&nbsp;Alberto Sensini ,&nbsp;Gordon Blunn","doi":"10.1016/j.engreg.2024.01.004","DOIUrl":"10.1016/j.engreg.2024.01.004","url":null,"abstract":"<div><p>The muscle tendon junction (MTJ) transmits the force generated by the muscle to the tendon and ultimately to the bone. Tears and strains commonly occur at the MTJ where regeneration is limited due poor vascularisation and the complexity of the tissue. Currently treatments for a complete MTJ tear are often unsuccessful. The creation of a tissue engineered MTJ would therefore be beneficial in the development of a novel treatment. In this study, aligned electrospun polycaprolactone fibres were fabricated and human myoblasts and tenocytes were cultured on the scaffold. The effect of 10 % cyclic strain and co-culture of myoblasts and tenocytes on the MTJ formation was investigated. The application of strain significantly increased cell elongation, and MTJ marker gene expression. Co-culture of myoblasts and tenocytes with strain induced higher MTJ marker gene expression compared with myoblasts and tenocytes cultured separately. Paxillin and collagen 22, naturally found in the MTJ, were also produced when cells were combined and grown in a 10 % strain environment. For the first time these results showed that the combination of the strain and co-culture of myoblasts and tenocytes promotes gene expression and production of proteins that are found in the MTJ.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 409-420"},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000045/pdfft?md5=55ab791f447bb5a77a770af7438e5fa5&pid=1-s2.0-S2666138124000045-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139634864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-talk between biometal ions and immune cells for bone repair","authors":"Shubo Liu ,&nbsp;Zhengjie Lin ,&nbsp;Wei Qiao ,&nbsp;Bin Chen ,&nbsp;Jie Shen","doi":"10.1016/j.engreg.2024.01.003","DOIUrl":"10.1016/j.engreg.2024.01.003","url":null,"abstract":"<div><p>Biometal ions are crucial in the structure and function of living organisms and have extensively been employed to promote bone tissue regeneration. Nevertheless, the biological functions of biometal ions and the underlying mechanisms responsible for their pro-regenerative effects remain incompletely understood, since bone repair is an intricate physiological process involving multiple cell types and signals. Recent accomplishments in the osteoimmunological field have revealed the momentous involvement of the immune system in mediating the therapeutic effects of biometal ions. The inflammatory factors secreted by immune cells contribute to bone cell migration, activation, and proliferation. This review summarizes the immune system and its constituent cells, followed by the current perspective on immunomodulation during bone healing. Next, the physicochemical and physiological properties of various biometal ions, including lithium, sodium, potassium, magnesium, calcium, strontium, vanadium, iron, cobalt, copper, and zinc, are thoroughly reviewed. In addition, the interactions between biometal ions, immune cells, and bone tissue are discussed, aiming to provide insights into the prospective development of novel approaches to bone tissue regeneration by harnessing the therapeutic potential of these biometal ions.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 375-408"},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000021/pdfft?md5=317cfda685dd368b344b03045d820968&pid=1-s2.0-S2666138124000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae-based drug delivery systems in biomedical applications","authors":"Hui Huang ,&nbsp;Yutong Lang ,&nbsp;Shoujie Wang ,&nbsp;Min Zhou","doi":"10.1016/j.engreg.2024.01.002","DOIUrl":"10.1016/j.engreg.2024.01.002","url":null,"abstract":"<div><p>Over decades of development, the modern drug delivery system continues to grapple with numerous challenges, including drug loading inefficiencies, issues of immunogenicity, and cytotoxicity. These limitations restrict its application across various systems. Microalgae, as a natural resource, are not only abundant in bioactive compounds but also possess multiple biological properties, including active surface, photosynthesis capabilities, and excellent biocompatibility. These attributes make microalgae highly promising as carriers for targeted drug delivery, offering significant potential for the diagnosis and treatment of various diseases. Therefore, leveraging the exceptional properties of microalgae for drug delivery and optimizing their qualities is of paramount importance. This article focuses on elucidating the biological characteristics of microalgae and their applications in drug delivery, with a particular emphasis on emerging strategies for efficient drug loading and precise targeted delivery. Microalgae, as a natural biomaterial, hold immense potential for both commercial and clinical applications.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 361-374"},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000033/pdfft?md5=a493ff0d8eca7ab0559446389aa566d9&pid=1-s2.0-S2666138124000033-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}