Engineered regeneration最新文献_第2页

Cell electrowriting: An advanced biofabrication approach for micrometre-scale living tissue fabrication 细胞电写作：一种用于微米尺度活组织制造的先进生物制造方法

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-01-19 DOI: 10.1016/j.engreg.2026.01.002

Harshavardhan Budharaju

引用次数: 0

Asini Corii Colla exhibits anti-photoaging efficacy via activating collagen synthesis and maintaining redox & extracellular matrix homeostasis Asini Corii Colla通过激活胶原合成，维持氧化还原和细胞外基质稳态，具有抗光老化的功效

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-07-14 DOI: 10.1016/j.engreg.2026.07.001

Xiao Xie, Zhijie Chen, Zesong Zhang, Zailin Chen, Cong Li, Jianling Zhang, Jiahao Yang, Zhihui Lu, Lei Jia, Meimei Fu, Jinshan Guo

{"title":"Asini Corii Colla exhibits anti-photoaging efficacy via activating collagen synthesis and maintaining redox & extracellular matrix homeostasis","authors":"Xiao Xie, Zhijie Chen, Zesong Zhang, Zailin Chen, Cong Li, Jianling Zhang, Jiahao Yang, Zhihui Lu, Lei Jia, Meimei Fu, Jinshan Guo","doi":"10.1016/j.engreg.2026.07.001","DOIUrl":"10.1016/j.engreg.2026.07.001","url":null,"abstract":"<div><div>Skin photoaging, primarily induced by chronic ultraviolet (UV) irradiation, is characterized by wrinkle formation, skin laxity, and collagen loss. As a traditional Chinese medicine, the potential of Asini Corii Colla (ACO) in protecting against skin photoaging has not been systematically investigated. This study aimed to comprehensively evaluate the anti-photoaging efficacy of ACO and elucidate its underlying mechanisms. Cytotoxicity and <em>in vitro</em> bioactivity of ACO were initially assessed in cultured human keratinocytes and dermal fibroblasts, followed by examination of its effects on cell proliferation and migration under normal conditions, and on SA-β-Gal activity, collagen, and elastin synthesis under UV-induced photoaging. Subsequently, a UV-induced skin photoaging mouse model was established to further validate the efficacy <em>in vivo</em>. Pathological changes, collagen content, and senescence-related markers in skin tissues were analyzed. <em>In vitro</em> results demonstrated that ACO, within a safe concentration range below 20 mg/mL, significantly promoted cell proliferation and migration, inhibited cellular senescence, enhanced collagen synthesis, and suppressed extracellular matrix (ECM) degradation. <em>In vivo</em> experiments confirmed that intervention with ACO markedly ameliorated UV-induced photoaging phenotypes in mice, including wrinkle formation and epidermal thickening, while maintaining dermal collagen density and content, and reducing oxidative stress in skin tissue. Collectively, ACO effectively mitigates UV-induced skin photoaging by promoting skin cell repair and regeneration, enhancing collagen synthesis, and maintaining redox homeostasis. These findings provide a solid scientific foundation for its potential application as an anti-photoaging agent.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 176-187"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148770018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Self-assembling peptide amphiphiles inhibit inflammation in a murine model of colitis 自组装肽两亲体在小鼠结肠炎模型中抑制炎症

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-09-12 DOI: 10.1016/j.engreg.2026.08.006

Amitha B. Shajahan, Matthew I. Bury, Neeti N. Gandhi, Simran M. Sharma, Brooke Hilderbrant, Norah M. Zimmerman, Aitran Le, Arun K. Sharma

{"title":"Self-assembling peptide amphiphiles inhibit inflammation in a murine model of colitis","authors":"Amitha B. Shajahan, Matthew I. Bury, Neeti N. Gandhi, Simran M. Sharma, Brooke Hilderbrant, Norah M. Zimmerman, Aitran Le, Arun K. Sharma","doi":"10.1016/j.engreg.2026.08.006","DOIUrl":"10.1016/j.engreg.2026.08.006","url":null,"abstract":"<div><div>Ulcerative colitis (UC) arises from complex interactions between genetic susceptibility, environmental triggers, and immune dysregulation, posing significant clinical and socioeconomic challenges. Current therapeutics cannot precisely target intestinal inflammation and cause deleterious side effects. Here, we investigate the local delivery of supramolecular anti-inflammatory peptide amphiphiles (AIF-PAs) as a biocompatible and comprehensive therapeutic for UC by administering these nanomolecules to an established IL-2 knockout (IL-2−/−) murine model that simulates human UC-like disease. Data derived from this study demonstrated that colonic lavage with AIF-PA1 and AIF-PA2 exhibit improved anatomical and physiological function including restoration of normal colonic architecture and transit times in the UC model compared to control groups. In particular, AIF-PA1 exhibited superior tissue repair characterized by enhanced epithelial regeneration, restoration of crypt architecture, and reduced inflammatory infiltrates compared to other treatments. Both AIF-PA treatments reduced pro-inflammatory cytokine secretion, including TNF-α, IFN-γ, and IL-1β up to 3-fold. Notably, AIF-PA1 resulted in a concomitant 5-fold increase in anti-inflammatory cytokine IL-10. Altogether, this data demonstrates that AIF-PAs can target and repair several aspects of UC pathology serving as a potential novel therapeutic for precisely mitigating an inflammatory intestinal milieu.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 276-285"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Synergistic interplay of dECM and exosomes in shaping the cartilage matrix microenvironment: A new paradigm for regenerative medicine dECM和外泌体在形成软骨基质微环境中的协同相互作用：再生医学的新范式

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-01-19 DOI: 10.1016/j.engreg.2026.01.003

Asif Shahriar , Song Chen , Yixuan Amy Pei , Ming Pei

{"title":"Synergistic interplay of dECM and exosomes in shaping the cartilage matrix microenvironment: A new paradigm for regenerative medicine","authors":"Asif Shahriar , Song Chen , Yixuan Amy Pei , Ming Pei","doi":"10.1016/j.engreg.2026.01.003","DOIUrl":"10.1016/j.engreg.2026.01.003","url":null,"abstract":"<div><div>This comprehensive review explores integrative approaches combining decellularized extracellular matrix (dECM) with exosome-based therapies to target structural integrity and biochemical signaling to promote cartilage regeneration. dECM is a biomimetic scaffold that retains essential components such as collagen and proteoglycans, which are crucial for cellular adhesion, matrix maintenance, and cartilage formation. Exosomes, on the other hand, derived from mesenchymal stem cells, enhance intercellular communication by delivering bioactive molecules such as growth factors and microRNAs. Combining dECM with exosomes can precisely mimic the cartilage matrix microenvironment, simultaneously promoting cell growth and the production of matrix components and modulating responses. This review delves into the mechanisms of dECM-exosome interactions, innovative integration technologies, and their impact on gene expression and cartilage-specific cellular responses. We demonstrate the potential of dECM-exosome therapy to enhance cartilage repair through <em>in vitro</em> and <em>in vivo</em> studies. Ultimately, these findings lay the foundation for the next generation of cell matrix-based therapeutics, which will provide advanced therapeutic strategies to improve clinical outcomes for cartilage damage and degenerative joint diseases.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 37-57"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147422956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

State-of-the-art molecular compounds in artificial salivary substitutes tackling xerostomia and oral mucositis 最先进的分子化合物在人工唾液替代品治疗口干症和口腔黏膜炎

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-07-18 DOI: 10.1016/j.engreg.2026.07.004

Jaggaiah N. Gorantla, Toan V. Phan, Chayada Piantham, Pirawish Limlawan, James R. Ketudat-Cairns, Jae-Yol Lim, Risa Chaisuparat, Joao N. Ferreira

{"title":"State-of-the-art molecular compounds in artificial salivary substitutes tackling xerostomia and oral mucositis","authors":"Jaggaiah N. Gorantla, Toan V. Phan, Chayada Piantham, Pirawish Limlawan, James R. Ketudat-Cairns, Jae-Yol Lim, Risa Chaisuparat, Joao N. Ferreira","doi":"10.1016/j.engreg.2026.07.004","DOIUrl":"10.1016/j.engreg.2026.07.004","url":null,"abstract":"<div><div>The oral mucosa relies on constant salivary lubrication as well as on epithelial and immune barriers to maintain its homeostasis and normal function. Disruption of these can be triggered by salivary gland (SG) damage following radiotherapy and/or chemotherapy for head and neck cancer (HNC) patients. This SG injury leads to major complications, particularly dry mouth (also known as xerostomia, XM) and oral mucositis (OM). Xerostomia with hyposalivation is associated with oral complications such as dental caries, dysphagia, candidiasis, and taste disturbances. In contrast, OM develops as a result of epithelial injury and inflammatory cascades, leading to pain, burning, altered dietary intake, and poor nutrition. Together, XM and OM severely compromise the quality of life of HNC patients worldwide. Currently, effective curative treatments remain limited due to the lack of functional SG tissue after HNC therapy, highlighting the urgent need for innovative strategies. Artificial salivary substitutes, ranging from biological to synthetic, provide a safe and effective means of enhancing oral hydration and lubrication. These formulations encompass traditional components like mucins and growth factors, as well as emerging agents based on lipids, carbohydrates, and plant-derived compounds. In this narrative review, we provide current insights into the protective biochemistry barriers of the oral mucosa elicited by XM and OM, and discuss emerging salivary mimetic compounds aimed at restoring mucosal homeostasis and immunoepithelial barrier functions.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 156-175"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148735529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Comparison of two hemostatic skin adhesive dressings, incorporating multi-metal bioactive glass” [Engineered Regeneration 6 (2025) 54-73] “两种含有多金属生物活性玻璃的止血皮肤粘合敷料的比较”[工程再生6(2025)54-73]的勘误表

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-04-03 DOI: 10.1016/j.engreg.2026.03.002

Melina Ghasemian, Neda Alasvand, Ali Samadikuchaksaraei, Hajir Bahrami, Mahmoud Azami, Farzad Ramroudi, Soheila Naderi Gharahgheshlagh, Hajar Nasiri, Soroush Taherkhani, Peiman Brouki Milan

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Nanogrooved plasma-activated PDMS functionalized with Argiope modesta spider silk extract enhances hWJ-MSC adhesion and chondrogenic differentiation 纳米槽等离子体激活PDMS与麻豆蛛丝提取物功能化增强hWJ-MSC粘附和软骨分化

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-09-08 DOI: 10.1016/j.engreg.2026.08.004

Candrani Khoirinaya, Karlia Meitha, Arie Wibowo, Arief Boediono, Toto Sudiro, Anggraini Barlian

{"title":"Nanogrooved plasma-activated PDMS functionalized with Argiope modesta spider silk extract enhances hWJ-MSC adhesion and chondrogenic differentiation","authors":"Candrani Khoirinaya, Karlia Meitha, Arie Wibowo, Arief Boediono, Toto Sudiro, Anggraini Barlian","doi":"10.1016/j.engreg.2026.08.004","DOIUrl":"10.1016/j.engreg.2026.08.004","url":null,"abstract":"<div><div>Cartilage tissue engineering requires biomaterial surfaces that support mesenchymal stem cell (MSC) adhesion and chondrogenic differentiation. This study investigated the effects of nanogrooved polydimethylsiloxane (PDMS) functionalized with <em>Argiope modesta</em> spider silk extract (SSE) following oxygen plasma treatment on the behavior of human Wharton’s Jelly-derived MSCs (hWJ-MSCs). Surface characterization by scanning electron microscopy (SEM), atomic force microscopy (AFM), water contact angle analysis, and X-ray photoelectron spectroscopy (XPS) confirmed the preservation of the nanogroove architecture, increased surface wettability following plasma treatment, and surface chemical changes associated with SSE functionalization. Liquid chromatography-high resolution mass spectrometry (LC<img>HRMS) analysis provisionally identified 189 protein accessions in the SSE. SSE-coated plasma-treated nanogrooved PDMS exhibited increased protein adsorption and higher cellular metabolic activity during early culture compared with uncoated controls, with 50 µg/mL SSE showing optimal cytocompatibility. SEM and confocal microscopy demonstrated enhanced cell attachment, spreading, and alignment along the nanogrooves, particularly on the 350-nm pattern. Under chondrogenic culture conditions, the NG-350-P-SSE substrate showed greater glycosaminoglycan accumulation, increased expression of SOX9, COL2A1, and ACAN, relatively lower COL10A1 expression, and stronger collagen II immunoreactivity than the control substrates. Collectively, these findings suggest that plasma-treated nanogrooved PDMS functionalized with SSE provides a favorable surface for hWJ-MSC chondrogenic differentiation and represents a promising biomaterial platform for further investigation in cartilage tissue engineering.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 241-258"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Physical properties of scaffold materials promote tissue repair and regeneration by influencing the paracrine action of mesenchymal stem cells 支架材料的物理特性通过影响间充质干细胞的旁分泌作用来促进组织修复和再生

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-09-03 DOI: 10.1016/j.engreg.2026.08.001

Yingjia Zhu, Chuanquan Liu, Ludvig J. Backman, Wei Zhang, Jialin Chen

{"title":"Physical properties of scaffold materials promote tissue repair and regeneration by influencing the paracrine action of mesenchymal stem cells","authors":"Yingjia Zhu, Chuanquan Liu, Ludvig J. Backman, Wei Zhang, Jialin Chen","doi":"10.1016/j.engreg.2026.08.001","DOIUrl":"10.1016/j.engreg.2026.08.001","url":null,"abstract":"<div><div>Mesenchymal stem cells (MSCs) have gained considerable attention in tissue engineering due to their remarkable multilineage differentiation potential, recruitment of endogenous cells, and orchestration of a regenerative microenvironment via the secretion of trophic factors. Contemporary research increasingly underscores that, rather than direct differentiation into injured tissue cells, MSCs primarily facilitate tissue repair through the secretion of bioactive paracrine factors. Notably, the physical properties of scaffold materials have emerged as key regulators of MSC paracrine function. This review provides a comprehensive overview of how diverse physical characteristics of scaffold materials modulate the composition and concentration of MSC-derived paracrine factors, thereby shaping the cellular microenvironment to enhance tissue regeneration. By elucidating the mechanistic interplay between scaffold physicality and MSC paracrine function, we aim to inform the rational design of biomaterials for optimized regenerative therapies.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 228-240"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Erratum regarding duplicated references in previously published articles in Volume 5- Volume 7 关于在第5卷-第7卷以前发表的文章中重复引用的勘误

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-09-17 DOI: 10.1016/j.engreg.2026.09.001

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Granular materials for biomedical applications: structural design, functional properties, advanced manufacturing and clinical application 生物医用颗粒材料：结构设计、功能特性、先进制造及临床应用

Engineered regeneration Pub Date : 2026-01-01 Epub Date: 2026-07-03 DOI: 10.1016/j.engreg.2026.06.001

Qifeng Guan, Jiakun Li, Kai Wang, Yufan Wang, Jiebo Li, Ping Li, Xufeng Niu, Sen Hou

{"title":"Granular materials for biomedical applications: structural design, functional properties, advanced manufacturing and clinical application","authors":"Qifeng Guan, Jiakun Li, Kai Wang, Yufan Wang, Jiebo Li, Ping Li, Xufeng Niu, Sen Hou","doi":"10.1016/j.engreg.2026.06.001","DOIUrl":"10.1016/j.engreg.2026.06.001","url":null,"abstract":"<div><div>Granular materials, comprising discrete macro/micro-particles exhibiting both solid- and fluid-like behaviors, have emerged as a transformative class of biomaterials owing to their tunable multiscale architectures and stimuli-responsive dynamics. Unlike bulk materials, their jamming-to-flow transition and shear-thinning behavior enable minimally invasive delivery through injection or spraying. These unique features drive transformative applications in biomedical area: (1) Tissue engineering and cell encapsulation leverage injectable granular scaffolds that endow porosity for nutrient diffusion and cell migration; (2) 3D bioprinting employs granular suspensions as self-supportive bioinks or self-healing matrices to stabilize fragile bioinks; (3) Drug delivery utilizes their modular feature for payload release with enhanced spatiotemporal precision; (4) Immunotherapy exploits engineered granular materials as synthetic antigen-presenting platforms to direct immune cell activation in vitro and in vivo. Key challenges in the application of granular materials include scalable monodisperse production of particles with designed microstructure, collective property prediction emerging from inter-particle interactions, expansion of their biomedical applications and translation of these materials to clinical practice. Emerging computational models and biohybrid designs promise advances in applying granular materials in organ-on-chip systems and adaptive medical implants. This review highlights the mechanistic synergy between granular physics and biomedical requirements, proposing a roadmap for designing next-generation therapeutic biomaterials.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"7 ","pages":"Pages 94-118"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148523842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0