Engineered regeneration最新文献_第2页

Comparison of two hemostatic skin adhesive dressings, incorporating multi-metal bioactive glass 两种含有多金属生物活性玻璃的止血皮肤粘合敷料的比较

Engineered regeneration Pub Date : 2024-07-01 DOI: 10.1016/j.engreg.2024.06.003

Melina Ghasemian, N. Alasvand, Ali Samadikuchaksaraei, Hajir Bahrami, Mahmoud Azami, Farzad Ramroudi, Soheila Naderi Gharegheshlaghi, Hajar Nasiri, Soroush Taherkhani, P. B. Milan

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Intelligent hydrogels for treating malignant melanoma 治疗恶性黑色素瘤的智能水凝胶

Engineered regeneration Pub Date : 2024-06-25 DOI: 10.1016/j.engreg.2024.05.004

Guopu Chen , Xiyu Wang , Jiaye Li , Ye Xu , Yue Lin , Fengyuan Wang

{"title":"Intelligent hydrogels for treating malignant melanoma","authors":"Guopu Chen , Xiyu Wang , Jiaye Li , Ye Xu , Yue Lin , Fengyuan Wang","doi":"10.1016/j.engreg.2024.05.004","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.05.004","url":null,"abstract":"<div><p>Malignant melanoma (MM) is an extremely aggressive and fatal form of skin cancer that primarily affects the bottom layer of the epidermis and is associated with poor clinical outcomes. Early-stage MM is typically treated through surgical removal, while chemotherapy and radiotherapy are common conventional treatment options that come with harmful side effects. Emerging therapies such as immunotherapy, photodynamic therapy, biologic therapy, and photothermal therapy present hopeful options for treatment due to their effective and secure drug delivery methods. To address the limitations of current treatment options, advanced methods of drug delivery for subcutaneous MM are being developed, with hydrogels emerging as a promising alternative. To date, significant advancements have been made in the treatment of MM through the use of hydrogels-based drug delivery systems through focal plastering, injection, implantation, and microneedles. Recent research on hydrogel-based drug delivery systems that integrate multiple therapies for the treatment of subcutaneous MM is discussed in this review.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 295-305"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000343/pdfft?md5=be9fd21c46e2f1979b1f682e961bce43&pid=1-s2.0-S2666138124000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rb1 improves human nonalcoholic fatty liver disease with liver organoids-on-a-chip 人参皂苷 Rb1 通过芯片上的肝脏器官改善人类非酒精性脂肪肝

Engineered regeneration Pub Date : 2024-06-21 DOI: 10.1016/j.engreg.2024.06.002

Hui Wang , Yue Zhu , Pengcheng Shi , Xiangyang Li , Qingyun Bu , Yachun Li , Xiaoyan You , Guoping Zhao

{"title":"Ginsenoside Rb1 improves human nonalcoholic fatty liver disease with liver organoids-on-a-chip","authors":"Hui Wang , Yue Zhu , Pengcheng Shi , Xiangyang Li , Qingyun Bu , Yachun Li , Xiaoyan You , Guoping Zhao","doi":"10.1016/j.engreg.2024.06.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.06.002","url":null,"abstract":"<div><p>Non-alcoholic fatty liver disease (NAFLD), a type of liver disease for which no treatment is currently approved, remains a major concern worldwide. It is manifested as simple hepatocyte steatosis and can develop into inflammation, fibrosis, cirrhosis and liver cancer in severe cases. However, due to the lack of appropriate <em>in vitro</em> drug testing platforms, an in-depth understanding of the therapeutic activity of ginsenoside Rb<sub>1</sub> in NAFLD remains challenging. Here, we proposed a NAFLD model on a liver organoids (LOs)-on-a-chip platform to evaluate the therapeutic effect of ginsenoside Rb<sub>1</sub> in a dynamic, multi-condition and high-throughput manner. This platform allowed us to reshape certain features such as multicellular types and liver-specific functions of the physiology of the human-relative liver. Free fatty acids (FFAs)-induced LOs displayed typical pathological characteristics of NAFLD progression, including steatosis, oxidative stress, lipid peroxidation, inflammation and fibrosis. With ginsenoside Rb<sub>1</sub> intervention, these pathological features can be significantly improved, which may provide new insights into the potential mechanisms of NAFLD progression and treatment and suggest the clinical implications for humans. The proposed system enables the formation, differentiation, and function of LOs to serve as a scalable, high-throughput and sensitive drug testing model, to potentially expedite the NAFLD drug discovery.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 283-294"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000355/pdfft?md5=9242bd2656ea0d7f1e8b7950d5896503&pid=1-s2.0-S2666138124000355-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum regarding updating Declaration of Competing Interest statements in previously published articles 关于更新以往发表文章中的竞争利益声明的勘误

Engineered regeneration Pub Date : 2024-06-01 DOI: 10.1016/j.engreg.2024.02.003

引用次数: 0

Immobilization of hUC-MSCs conditioned medium on 3D PLLA collagen-coated matrix enhances diabetic wound healing progression 将 hUC-MSCs 条件培养基固定在三维 PLLA 胶原包覆基质上可促进糖尿病伤口愈合进展

Engineered regeneration Pub Date : 2024-05-08 DOI: 10.1016/j.engreg.2024.04.005

Siufui Hendrawan , Olivia Marcelina , Sukmawati Tansil Tan , Hans Ulrich Baer

{"title":"Immobilization of hUC-MSCs conditioned medium on 3D PLLA collagen-coated matrix enhances diabetic wound healing progression","authors":"Siufui Hendrawan , Olivia Marcelina , Sukmawati Tansil Tan , Hans Ulrich Baer","doi":"10.1016/j.engreg.2024.04.005","DOIUrl":"10.1016/j.engreg.2024.04.005","url":null,"abstract":"<div><p>Conditioned medium (CM) derived from human umbilical cord-mesenchymal stem cells (hUC-MSCs) which contains numerous amounts of growth factors, has demonstrated potential in treatment of diabetic wounds. However, for practical application, a biodegradable supporting material is needed to hold the CM and fill in the injury site, where deep cavity wounds are often present in diabetic patients. Poly-<span>l</span>-lactic acid matrix coated with collagen (PLLA/CC) is a suitable carrier due to its biodegradability and biocompatibility. Thus, we present a method to immobilize the hUC-MSCs CM on PLLA/CC through freeze-drying process (PLLA/CC CM FD). When seeded on PLLA/CC CM FD, fibroblasts had an increased cellular function in producing collagen; although no enhancement in cell viability was observed. Moreover, implantation of PLLA/CC CM FD on the wound of diabetic rats showed improvement in wound closure and collagen deposition in the wound area. Altogether, this study exhibits the potential of PLLA/CC CM FD as a therapy for diabetic deep cavity wound.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 421-431"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000239/pdfft?md5=39d2a2a4e1fdbcf5b4cc3dfedc2e22ca&pid=1-s2.0-S2666138124000239-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141046404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution 人工椎间盘核及其他髓核替代策略：新兴手术解决方案的过去、现在和未来设计

Engineered regeneration Pub Date : 2024-05-08 DOI: 10.1016/j.engreg.2024.04.006

Greg Sacks, Vincent DeStefano, Claire Parker, Ryan Lebens, Harry Mushlin

{"title":"The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution","authors":"Greg Sacks, Vincent DeStefano, Claire Parker, Ryan Lebens, Harry Mushlin","doi":"10.1016/j.engreg.2024.04.006","DOIUrl":"10.1016/j.engreg.2024.04.006","url":null,"abstract":"<div><p>Nucleus Pulposus (NP) Replacement is a developing surgical methodology for the treatment of pathology related to degeneration of intervertebral discs (IVDs). This article provides necessary context regarding the pathologies treated with this technology, the biomechanical structure and function of the IVD, and the procedures this technology aims to replace. Primarily, it provides an overview and discussion of commercial and experimental preformed and in situ curing prosthesis designs reported in the scientific literature and summarizes the results of biomechanical and clinical studies evaluating their efficacy. Contextual and updated information on the most recent research into NP replacement with novel hydrogel and tissue engineering (TE) strategies is described. Replacement of the NP allows for potential improvement in the treatment of degenerative spinal pathologies through minimally invasive surgical techniques.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 269-281"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000240/pdfft?md5=8b9369e4f3903991157a218650dcbc79&pid=1-s2.0-S2666138124000240-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma LSM6 受 HMGB1 调控，促进喉鳞状细胞癌细胞的增殖和迁移

Engineered regeneration Pub Date : 2024-04-24 DOI: 10.1016/j.engreg.2024.04.004

Dengbin Ma , Jiayi Li , Hui Li , Yao Tang , Xia Gao , Hong Chen , Xiaoyun Qian , Xiaohui Shen

{"title":"LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma","authors":"Dengbin Ma , Jiayi Li , Hui Li , Yao Tang , Xia Gao , Hong Chen , Xiaoyun Qian , Xiaohui Shen","doi":"10.1016/j.engreg.2024.04.004","DOIUrl":"10.1016/j.engreg.2024.04.004","url":null,"abstract":"<div><p>Elevated levels of high mobility group protein B1 (HMGB1) play a significant role in the pathogenesis of many diseases, but is particularly important for the formation of malignant tumors. Nonetheless, the function of HMGB1 and the underlying mechanism of laryngeal squamous cell carcinoma (LSCC) remain incompletely understood, causing uncertainty. Here we found immunohistochemistry from 97 LSCC tissues showed HMGB1 was upregulated, which was associated with poor differentiation. HMGB1 knockdown could significantly inhibit wound closure and colony formation. The full-genome gene expression microarray was performed to investigate the mechanism. After knockdown of HMGB1 by siRNA, among the expressed differential genes, 10 genes were randomly selected for validation. Then, shRNA lentivirus targeting these genes were constructed to explore their role in LSCC by cell proliferation assay. LSM6 downregulation was dramatically promoted by HMGB1 knockdown, resulting in higher expression in LSCC tissues. Furthermore, downregulation of LSM6 could significantly suppress cell proliferation, migration and colony formation. This study indicated that HMGB1 promoted LSCC cell malignant phenotypes through regulation of LSM6. We anticipate that HMGB1-LSM6 could be a putative therapeutic target for LSCC.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 247-254"},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000227/pdfft?md5=9818316e958ae374afc05d8675caad55&pid=1-s2.0-S2666138124000227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140758603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of gut microbiota associated metabolites in digestive disorders 肠道微生物群相关代谢物在消化系统疾病中的作用

Engineered regeneration Pub Date : 2024-04-16 DOI: 10.1016/j.engreg.2024.04.003

Na Li , Cheng Zhao , Pingnan Zhang , Songting Wu , Xiaotan Dou , Saifei Xu , Xiaoqi Zhang , Chunyan Peng , Ying Xie , Shuling Huang , Lin Zhou , Yonghua Shen , Lei Wang , Jinglin Wang , Chenggong Yu

{"title":"The role of gut microbiota associated metabolites in digestive disorders","authors":"Na Li , Cheng Zhao , Pingnan Zhang , Songting Wu , Xiaotan Dou , Saifei Xu , Xiaoqi Zhang , Chunyan Peng , Ying Xie , Shuling Huang , Lin Zhou , Yonghua Shen , Lei Wang , Jinglin Wang , Chenggong Yu","doi":"10.1016/j.engreg.2024.04.003","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.04.003","url":null,"abstract":"<div><p>The gut has been a focal point in the research of digestive system disorders. The internal microbiota generates metabolites that function as signaling molecules and substrates, interacting with the intestinal wall and influencing host physiology and pathology. Besides, the gut microbiota and metabolites owe highly diverse types and quantities, posing challenges for quantitative analysis, and monitoring frequent interactions between digestive tract metabolites and the intestinal wall remains a challenge. However, research targeting gut microbiota metabolites has elucidated their relevance to digestive diseases. By modulating metabolites such as short-chain fatty acids, bile acids, and lipopolysaccharides, it is possible to intervene in the progression of diseases such as inflammatory bowel disease and non-alcoholic fatty liver disease. Currently, research on gut microbiota is advancing, and more work is required to explore the interactions between host, microbes and underlying mechanisms. In this review, we have revisited the generation of gut microbiota-related metabolites, their impact on diseases, and modes of interaction, emphasizing the significant role of metabolites in digestive system disorders. It is believed that the linkage between gut microbiota and diseases in current research can be established through metabolites, providing a framework and foundation for research in the field of metabolomics and fundamental mechanisms.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 228-246"},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000215/pdfft?md5=15251ba8b0411969ab5a166a0be25dad&pid=1-s2.0-S2666138124000215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140649206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained adenosine release: Revealing its impact on osteogenic signalling pathways of human mesenchymal stromal cells 持续腺苷释放：揭示其对人类间充质基质细胞成骨信号通路的影响

Engineered regeneration Pub Date : 2024-04-12 DOI: 10.1016/j.engreg.2024.04.002

Hadi Hajiali , Jane McLaren , Cristina Gonzalez-García , Salah Abdelrazig , Dong-Hyun Kim , Matthew J. Dalby , Manuel Salmerón-Sánchez , Felicity R.A.J. Rose

{"title":"Sustained adenosine release: Revealing its impact on osteogenic signalling pathways of human mesenchymal stromal cells","authors":"Hadi Hajiali , Jane McLaren , Cristina Gonzalez-García , Salah Abdelrazig , Dong-Hyun Kim , Matthew J. Dalby , Manuel Salmerón-Sánchez , Felicity R.A.J. Rose","doi":"10.1016/j.engreg.2024.04.002","DOIUrl":"10.1016/j.engreg.2024.04.002","url":null,"abstract":"<div><p>Non-healing fractures, a global health concern arising from trauma, osteoporosis, and tumours, can lead to severe disabilities. Adenosine, integral to cellular energy metabolism, gains prominence in bone regeneration via adenosine A<sub>2</sub>B receptor activation. This study introduces a controlled-release system for localized adenosine delivery, fostering human mesenchymal stromal cell (hMSC) differentiation into functional bone cells. The study investigates how the ratio of lactic acid to glycolic acid in microparticles can influence adenosine release and explores the downstream effects on gene expression and metabolic profiles of osteogenic differentiation in hMSCs cultured in growth and osteoinductive media. Insights into adenosine-modulated signalling pathways during MSC differentiation, with osteogenic factors, provide a comprehensive understanding of the pathways involved. Analysing gene expression and metabolic profiles unravels adenosine's regulatory mechanisms in MSC differentiation. Sustained adenosine release from microparticles induces mineralization, synergizing with osteogenic media supplements, showcasing the potential of adenosine for treating critical bone defects and metabolic disorders. This study highlights the efficacy of a polymeric microparticle-based delivery system, offering novel strategies for bone repair. Unveiling adenosine's roles and associated signalling pathways advances our comprehension of molecular mechanisms steering bone regeneration, propelling innovative biomaterial, combined with metabolites, approaches for clinical use.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 255-268"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000197/pdfft?md5=17905847100ef2d7f7ea28343ba27e41&pid=1-s2.0-S2666138124000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140764298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted delivery of factors and cells for improving cardiac tissue regeneration and heart function following myocardial infarction 靶向输送因子和细胞，改善心肌梗死后的心脏组织再生和心脏功能

Engineered regeneration Pub Date : 2024-04-12 DOI: 10.1016/j.engreg.2024.04.001

Kamila Raziyeva, Zharylkasyn Zharkinbekov , Yevgeniy Kim , Arman Saparov

{"title":"Targeted delivery of factors and cells for improving cardiac tissue regeneration and heart function following myocardial infarction","authors":"Kamila Raziyeva, Zharylkasyn Zharkinbekov , Yevgeniy Kim , Arman Saparov","doi":"10.1016/j.engreg.2024.04.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.04.001","url":null,"abstract":"<div><p>Following myocardial infarction (MI), cardiac tissue undergoes irreversible cellular alterations, with cardiomyocytes being replaced by fibrotic tissue. In order to improve tissue regeneration, a previously characterized chitosan-based cryogel, which was designed by our group, was used. The treatment regimen involved the sequential delivery of the cryogel loaded with specific cytokines and growth factors, followed by a separate injection of pre-differentiated cells. Initially, the cryogel loaded with interleukin-10 and transforming growth factor-β was injected into infarcted tissue immediately after MI induction, targeting the acute inflammatory response. On day four post-MI, a second injection was administered, this time utilizing cryogel loaded with vascular endothelial growth factor and fibroblast growth factor-2, aimed at promoting tissue regeneration and angiogenesis. Subsequently, on day six post-MI, the experimental group received cardiomyocyte-like cells, smooth muscle cells, and endothelial cells. The purpose of these cells, in synergy with cytokines and growth factors, was to repopulate the lost cellular populations, thereby enhancing myocardial repair. The treatment improved myocardial tissue regeneration, increased cardiac output, ejection fraction, and reduced fibrotic regions. Thus, the chitosan-based cryogel, enriched with anti-inflammatory and proangiogenic factors and supplemented with pre-differentiated cells, offers a promising platform for controlled release of therapeutics, promoting substantial tissue repair and regeneration following MI.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 210-227"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000203/pdfft?md5=47705ae25b613b976958c8c325ea45b2&pid=1-s2.0-S2666138124000203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140551241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0