Engineered regeneration最新文献_第4页

ORM1 mediates osteoblast/osteoclast crosstalk in adolescent idiopathic scoliosis via RANKL/OPG ratio alteration ORM1通过改变RANKL/OPG比值介导青少年特发性脊柱侧凸的成骨细胞/破骨细胞串扰

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2024-08-06 DOI: 10.1016/j.engreg.2024.07.002

Kai Chen , Mingyuan Yang , Beier Luo , Xiaolong Li , Xiao Zhai, Xiaoyi Zhou, Yushu Bai, Ziqiang Chen, Kai Chen, Ming Li

{"title":"ORM1 mediates osteoblast/osteoclast crosstalk in adolescent idiopathic scoliosis via RANKL/OPG ratio alteration","authors":"Kai Chen , Mingyuan Yang , Beier Luo , Xiaolong Li , Xiao Zhai, Xiaoyi Zhou, Yushu Bai, Ziqiang Chen, Kai Chen, Ming Li","doi":"10.1016/j.engreg.2024.07.002","DOIUrl":"10.1016/j.engreg.2024.07.002","url":null,"abstract":"<div><div>Adolescent idiopathic scoliosis (AIS), a complex early-onset three-dimensional spinal deformity, remains etiologically ambiguous despite extensive ongoing investigations. Currently, braces and surgeries are primary treatments of AIS, which come with inherent risks and costs. Therefore, there is an urgent need for biotherapeutic targets for AIS. Using human specimens obtained from the clinic, we discovered that ORM1 was expressed in AIS bone tissues. Also, immune cells were found to interact with osteoclasts through the LTB-LTBR pathway, resulting in elevated ORM1 expression, proliferation promotion and differentiation of monocytes/osteoclasts. Protein analysis showed that in ORM1-positive AIS patient-derived osteoblasts, there was an increased expression of RANKL, decreased expression of OPG, and an increased RANKL/OPG ratio. Furthermore, osteoclasts overexpressing ORM1 promoted their own differentiation while inhibiting osteoblast proliferation and function. ORM1 knockdown osteoclasts co-cultured with osteoblasts, along with the addition of leptin, significantly inhibited osteoclast differentiation while promoting osteoblast proliferation and function-related protein expression. In conclusion, ORM1 acts as a detrimental factor in the pathogenesis of Adolescent Idiopathic Scoliosis (AIS) by promoting osteoclast differentiation and inhibiting both the proliferation and function of osteoblasts. This suggests that ORM1 may represent a valuable therapeutic target for AIS.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 45-53"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Force induces axon growth in inhibitory conditions 力在抑制条件下诱导轴突生长

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-06-06 DOI: 10.1016/j.engreg.2025.05.003

Elena Capitanini , Laura Talarico , Sara De Vincentiis , Chiara Giacomelli , Sara Vitolo , Lorenzo Da Palmata , Laura Marchetti , Elisabetta Ferraro , Maria Letizia Trincavelli , Vittoria Raffa

{"title":"Force induces axon growth in inhibitory conditions","authors":"Elena Capitanini , Laura Talarico , Sara De Vincentiis , Chiara Giacomelli , Sara Vitolo , Lorenzo Da Palmata , Laura Marchetti , Elisabetta Ferraro , Maria Letizia Trincavelli , Vittoria Raffa","doi":"10.1016/j.engreg.2025.05.003","DOIUrl":"10.1016/j.engreg.2025.05.003","url":null,"abstract":"<div><div>Axon navigation is guided by spatial patterns of chemical and physical cues in the developing central nervous system. Following injury, these patterns are disrupted, the microenvironment evolves rapidly, and inhibitory molecules create a barrier to the regeneration of severed axons. We have recently developed a technology called nano-pulling designed to stimulate axon growth and regeneration by modulating neuronal mechanotransduction. In this paper, we demonstrate that nano-pulling can induce axon growth in hippocampal neurons even in the presence of repulsive cues, such as chondroitin sulfate proteoglycans, semaphorin 3A, microglial activation, and pro-inflammatory cytokines. Nano-pulling can also enhance the elongation of neural processes in neural progenitors transplanted into an organotypic spinal cord injury model that mimics the tissue complexity and inflammation seen in <em>in vivo</em> models. Our data suggest that nano-pulling could be used as a strategy to manipulate axon growth, overcoming certain extrinsic inhibitory factors.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 133-145"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering neuroblastoma models for clinical translation 用于临床翻译的神经母细胞瘤工程模型

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-06-06 DOI: 10.1016/j.engreg.2025.05.002

Taiyu Song , Rui Liu , Feika Bian , Bin Kong , Jingjing Gan

{"title":"Engineering neuroblastoma models for clinical translation","authors":"Taiyu Song , Rui Liu , Feika Bian , Bin Kong , Jingjing Gan","doi":"10.1016/j.engreg.2025.05.002","DOIUrl":"10.1016/j.engreg.2025.05.002","url":null,"abstract":"<div><div>Neuroblastoma is a profoundly heterogenous extracranial solid tumor in pediatric patients. Elevated risk grade of neuroblastoma has been correlated with unsatisfactory prognosis and resistance to chemotherapy. Despite multimodal therapies exploited for killing neuroblastoma tumor cells, in high-risk neuroblastoma patients, the long-term survival is currently less than 50%. Promising approaches to evaluating the extent of heterogeneity via gene expression profiling of cell subpopulations within individual tumors are still lacking. There is uncertainty about the cross-talk between neuroblastoma cells and other non-neoplastic cell components in the tumor microenvironment. Recently, concerns about individualized eradication therapies have advanced the demand for the diversified construction of neuroblastoma tumor models. This review briefly introduces the genetic variation, subpopulations, and tumor microenvironment aspects of neuroblastoma heterogeneity. Then, we summarize recent methods of constructing tumor models to mimic the biological features of neuroblastoma tumors <em>in vitro</em>. Finally, we conclude the future trends and perspectives in neuroblastoma tumor therapy.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 146-159"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs and mRNAs: A comparative analysis in translation and commercialization MicroRNAs和mrna：翻译和商业化的比较分析

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1016/j.engreg.2025.04.001

Jamie Cheng , Wubin Bai , Thomas Caranasos

{"title":"MicroRNAs and mRNAs: A comparative analysis in translation and commercialization","authors":"Jamie Cheng , Wubin Bai , Thomas Caranasos","doi":"10.1016/j.engreg.2025.04.001","DOIUrl":"10.1016/j.engreg.2025.04.001","url":null,"abstract":"<div><div>Success with mRNA vaccines has positioned microRNAs as possibly the nextgeneration therapeutics. While mRNAs have demonstrated rapid translation into effective vaccines, microRNAs possess certain advantages in gene regulation, particularly in targeting multiple genes concurrently. Therefore, they are promising for the treatment of cancer and cardiovascular disorders. This paper provides information about microRNAs with regard to their therapeutic applications, based on their stability, tissue distribution, and specific gene modulation. However, commercialization of microRNA also faces challenges, especially in aspects of delivery and regulatory approval. Unlike the mRNAs, which benefit from the delivery method of lipid nanoparticles, the microRNAs need an innovative delivery method that guarantees target efficacy and ensures minimal off-target effects. Additionally, regulatory pathways for microRNAs have not been fully developed and have to be well-assessed for their safety and effectiveness. We propose that microRNAs and mRNAs may exert complementary therapeutic functions, as mRNAs in vaccines are superior while microRNAs offer new roads to treatments in multifactorial diseases. Since these challenges are being overcome, microRNA-based therapies have the potential to revolutionize treatments for gene regulation and thereby affect the future of RNA therapeutics. This paper outlines the therapeutic potential of microRNAs and the steps involved in translation into clinical application.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 230-234"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulating red blood cell coating for mitigation of foreign body reactions 免疫调节红血球涂层减轻异物反应

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-10-10 DOI: 10.1016/j.engreg.2025.09.001

Yi Ye , Jinyun Wu , Tao Sun , Minghong Huang , Jiaping Pan , Danjing Hu , Keying Chen , Dawei Wang , Chenxi Wang , Yangkun Shen , Qi Chen , Baolin Huang

{"title":"Immunomodulating red blood cell coating for mitigation of foreign body reactions","authors":"Yi Ye , Jinyun Wu , Tao Sun , Minghong Huang , Jiaping Pan , Danjing Hu , Keying Chen , Dawei Wang , Chenxi Wang , Yangkun Shen , Qi Chen , Baolin Huang","doi":"10.1016/j.engreg.2025.09.001","DOIUrl":"10.1016/j.engreg.2025.09.001","url":null,"abstract":"<div><div>Chronic inflammation of biomedical implants usually leads to fibrosis and device malfunction in the long term. To address these issues, a cell-crosslinked coating of red blood cells (RBCs) was developed to imitate a self-friendly biological membrane and camouflage the implants from immune system. Using the widely applied poly(dimethylsiloxane) (PDMS) as a model substrate, a natural polymer hyaluronic acid (HA) layer was constructed upon PDMS (PDMS-HA), which was further decorated with RBCs (PDMS-HA-RBC). Compared to pristine PDMS, both PDMS-HA and PDMS-HA-RBC notably polarized the original macrophages into an anti-inflammatory phenotype (M2) rather than a pro-inflammatory phenotype (M1). Especially, PDMS-HA-RBC exhibited the highest M2/M1 ratios of macrophages, suggesting efficient modulation effects of inflammation reactions by the RBCs coating. Moreover, <em>in vivo</em> results found that PDMS induced considerable foreign body reactions (FBRs) and extensive fibrosis formation. In contrast, PDMS-HA revealed a significantly thinner fibrotic layer while PDMS-HA-RBC induced the least amount of fibrosis. In addition, PDMS-HA-RBC exhibited the highest fluorescent intensity of CD206 (M2 antigen) and the lowest fluorescent intensity of CD86 (M1 antigen). It was speculated that the RBCs coating-mediated macrophage polarization was mainly attributed to the presence of immune escape antigens (such as CD47 and CD59) upon the cell coating. Altogether, our living RBCs coating demonstrated significant potentials in mitigating FBRs of PDMS, indicating their promising applications in surface engineering of various biomedical implants.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 218-229"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonic manipulation in tissue engineering 组织工程中的超声操作

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-03-14 DOI: 10.1016/j.engreg.2025.03.001

Zijun Guan, Jianbo Huang, Yang Gao, Hongju Zhou, Liyun Wang, Lang Ma, Li Qiu

{"title":"Ultrasonic manipulation in tissue engineering","authors":"Zijun Guan, Jianbo Huang, Yang Gao, Hongju Zhou, Liyun Wang, Lang Ma, Li Qiu","doi":"10.1016/j.engreg.2025.03.001","DOIUrl":"10.1016/j.engreg.2025.03.001","url":null,"abstract":"<div><div>Ultrasonic waves exert radiation force on cells and other cell size particles, applied in particle manipulation, growth factor delivery, substance cavitation, and single cell tweezing. Featuring in the safe, contactless, precise, and tunable properties and advantages, ultrasonic waves can be used to control cell's locations aiding in the morphogenesis of complex cell systems, which will be widely used in the future generation tissue engineering. In this review, we summarized current application of ultrasonic waves in the field of cell manipulation and tissue engineering. First, we briefly introduced the physical mechanisms of cell manipulation and described the five kinds of device designs including holographic device, tweezer device, stream standing wave device, surface acoustic wave device, and bulk acoustic wave device. Secondly, we concluded recent works to culture tissue cells in certain spatial patterns using ultrasonic device including bone tissue, cartilage, cardiac muscle, skeletal muscle, endothelial, and neurons. Finally, we systematically highlighted the current challenges and future perspectives. It is believed that this cutting review will substantially stimulate the development and widespread utilization of ultrasonic standing wave in future tissue engineering applications.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 74-84"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Mapping the trajectory of organoid research: Insights into complex models and advanced technologies 绘制类器官研究的轨迹：对复杂模型和先进技术的见解

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-11-06 DOI: 10.1016/j.engreg.2025.11.003

Yiming Zhang , Lianyong Wang , Qingqian Zhao , Jinhao Gong , Ye Tian , Yang Liu , Shanfeng Chen , Xin Zhao , Qiang Yang , Yanhong Zhao

{"title":"Mapping the trajectory of organoid research: Insights into complex models and advanced technologies","authors":"Yiming Zhang , Lianyong Wang , Qingqian Zhao , Jinhao Gong , Ye Tian , Yang Liu , Shanfeng Chen , Xin Zhao , Qiang Yang , Yanhong Zhao","doi":"10.1016/j.engreg.2025.11.003","DOIUrl":"10.1016/j.engreg.2025.11.003","url":null,"abstract":"<div><div>In recent years, organoid technology has become a pivotal advancement in stem cell research, offering an unprecedented experimental platform that mimics the morphology and function of human organs, pushing modern life sciences to the forefront. This technology has successfully developed complex three-dimensional (3D) structures derived from human pluripotent or adult stem cells, closely resembling natural organs in tissue and function. Organoid models facilitate in-depth studies of organ development, disease progression, and drug interactions, effectively addressing ethical and practical limitations in traditional biomedical research. Despite its promising development, organoid technology faces challenges in practical applications, particularly in constructing complex organs like the brain, heart, and bone tissue. Therefore, we conducted a bibliometric analysis of 8051 publications on organoids from the Web of Science Core Collection (WoSCC) database (2000-2025). These studies were categorized into five clusters related to current situations and future application directions. Based on our bibliometric analysis, systematically summarize the development of organoid technologies, from laboratory to preclinical applications, exploration of complex organoids, and advanced technologies, while analyzing their limitations and prospects. This review will provide an objective perspective to identify current trends in organoid research and provide new insights into the future research and clinical applications of organoids by teasing out the past-present of organoid research.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 264-276"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Conductive PS inverse opals for regulating proliferation and differentiation of neural stem cells” [Engineered Regeneration 4 (2023) 214-221] “用于调节神经干细胞增殖和分化的导电PS逆蛋白石”的勘误表[工程再生4 (2023)214-221]

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-12-21 DOI: 10.1016/j.engreg.2025.12.001

Yangnan Hu , Han Zhang , Hao Wei , Menghui Liao , Xiaoyan Chen , Jiayue Xing , Lian Duan , Cuntu Cheng , Weicheng Lu , Xuechun Yang , Peina Wu , Huan Wang , Jingdun Xie , Renjie Chai

引用次数: 0

Transcript-activated collagen matrix for enhanced bone marrow stem cell differentiation and osteochondral repair 转录活化胶原基质增强骨髓干细胞分化和骨软骨修复

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-04-03 DOI: 10.1016/j.engreg.2025.03.002

Gang Zhong , Yixuan Luo , Meng Wang , Zhengran Yu , Xuenong Zou , Gang Wang , Fei Chen , Yin Yu

{"title":"Transcript-activated collagen matrix for enhanced bone marrow stem cell differentiation and osteochondral repair","authors":"Gang Zhong , Yixuan Luo , Meng Wang , Zhengran Yu , Xuenong Zou , Gang Wang , Fei Chen , Yin Yu","doi":"10.1016/j.engreg.2025.03.002","DOIUrl":"10.1016/j.engreg.2025.03.002","url":null,"abstract":"<div><div>The regeneration of critical-sized osteochondral defects remains a significant challenge due to the limited self-healing capacity of cartilage. Traditional approaches, such as autologous chondrocyte implantation (ACI) and matrix-induced autologous chondrocyte implantation (MACI), have shown promise but are limited by issues like insufficient cell availability, dedifferentiation of chondrocytes during expansion, and the formation of fibrocartilage rather than functional hyaline cartilage. This study presents a promising approach utilizing transcript-activated matrices (TAMs) with mRNA to enhance the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) in situ. Chemically modified mRNA (cmRNA) encoding transforming growth factor β3 (TGF-β3) was encapsulated in a collagen hydrogel to provide localized, sustained delivery of chondrogenic signals. In a rat model of critical-sized osteochondral defects, this strategy significantly promoted cartilage regeneration, achieving structural and molecular restoration within six weeks. Histological and biochemical analyses revealed robust chondrogenesis, enhanced extracellular matrix deposition, and superior mechanical properties. Moreover, TAM therapy maintained subchondral bone integrity This work highlights the transformative potential of mRNA-activated matrices as a platform technology that not only addresses key limitations of existing cartilage repair strategies but also provides a biomimetic microenvironment that guides stem cell differentiation and tissue regeneration.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 111-120"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current advances and prospects in biomaterials-guided tools for liver organoids research 生物材料导向类肝器官研究工具的现状与展望

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.1016/j.engreg.2025.07.004

Sana Ahmed , Eman Alshehri , Sarah Nazneen , Fayrouz Attia , Dalia Obeid , Hanan Almuzaini , Alaa Alzahrani , Jahan Salma , Iriya Fujitsuka , Abdullah M. Assiri , Dieter C. Broering , Raja Chinnappan , Ahmed Yaqinuddin , Tanveer Ahmad Mir

{"title":"Current advances and prospects in biomaterials-guided tools for liver organoids research","authors":"Sana Ahmed , Eman Alshehri , Sarah Nazneen , Fayrouz Attia , Dalia Obeid , Hanan Almuzaini , Alaa Alzahrani , Jahan Salma , Iriya Fujitsuka , Abdullah M. Assiri , Dieter C. Broering , Raja Chinnappan , Ahmed Yaqinuddin , Tanveer Ahmad Mir","doi":"10.1016/j.engreg.2025.07.004","DOIUrl":"10.1016/j.engreg.2025.07.004","url":null,"abstract":"<div><div>Liver is the largest solid organ in the human body engaged in an array of critical physiological activities that primarily support metabolism, digestion, nutrient storage, detoxification. Liver dysfunction due to disease or surgical intervention often leads to severe life-threatening complications or death in humans. Therefore, in vitro liver models that mimic key functional characteristics are considered a reliable option for the study of liver diseases and the development of new therapeutic agents. Furthermore, they can overcome the limitations of conventional monolayer cultures and animal related experiments in assessing the response of new therapeutic agents and drug molecules. In recent years, the emergence and advancement of organoid technology has greatly facilitated the development of reliable in vitro liver models for a variety of biomedical and pharmacological applications. However, organoid culture primarily relies on tumor-derived extracellular matrix, such as Matrigel, which pose challenges due to its xenogeneic nature and variable composition. Therefore, creating organoid models using Matrigel-free hydrogel materials could significantly improve the outcomes of regenerative medicine and experimental studies. In this review, we provide an overview of rapidly evolving biomaterials for organoid research. We then outline preparation methods and the most relevant studies applying different hydrogels for engineering liver organoid models. Finally, we discuss the challenges, future perspectives, and opportunities of hydrogels in engineering next-generation liver organoid models for translational applications.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 203-217"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0