Engineered regeneration最新文献

{"title":"Novel injectable composite incorporating denosumab promotes bone regeneration via bone homeostasis regulation","authors":"Guang Yang ,&nbsp;Zili Guo ,&nbsp;Xiangfeng Zhang ,&nbsp;Jiayu Chen ,&nbsp;Jie Weng ,&nbsp;Jiapeng Bao ,&nbsp;Xiaohua Yu","doi":"10.1016/j.engreg.2024.05.001","DOIUrl":"10.1016/j.engreg.2024.05.001","url":null,"abstract":"<div><div>Repair of large bone defects remains to be clinically challenging, yet current bone repair strategies focus on optimizing the osteogenic capacity of bone grafts, while the role of osteoclasts in bone regeneration has been largely ignored. Herein, we designed a injectable self-curing bone grafting paste capable of regulating both anabolic/catabolic activities during bone healing by immobilizing the RANKL inhibitor denosumab on dermal-derived extracellular matrix (ECM) microfibres, which were then incorporated into an injectable paste via a hydration reaction between β-tricalcium phosphate (β-TCP), monocalcium phosphate monohydrate (MCPM) and calcium sulfate hemihydrate (CSH). The incorporation of ECM microfibres not only serves as a sustained-release denosumab carrier to inhibit osteoclastogenesis but also improves the mechanical properties of the resulting composite by increasing the interaction between the organic and inorganic phases. <em>In vitro</em>, calcium supply from the composite along with ECM enhanced osteogenic differentiation of BMSC while release of denosumab effectively inhibits osteoclast fusion and alleviate osteoclastic activity. <em>In vivo</em>, it was observed that CSH/CP@ECM-Deno significantly reduced the number of osteoclasts, slowed down the process of bone resorption, and accelerated collagen deposition to promote new bone generation. These results suggest that modulation of osteoclastogenesis by interfering with bone homeostasis may be an effective bone repair strategy.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 482-494"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A patch comprising human umbilical cord-derived hydrogel and mesenchymal stem cells promotes pressure ulcer wound healing","authors":"Liqin Chen ,&nbsp;Ying Zhang ,&nbsp;Kexin Wang ,&nbsp;Meixian Jin ,&nbsp;Qi Chen ,&nbsp;Simin Wang ,&nbsp;Wei Hu ,&nbsp;Zhai Cai ,&nbsp;Yang Li ,&nbsp;Shao Li ,&nbsp;Yi Gao ,&nbsp;Shuqin Zhou ,&nbsp;Qing Peng","doi":"10.1016/j.engreg.2024.10.002","DOIUrl":"10.1016/j.engreg.2024.10.002","url":null,"abstract":"<div><div>Pressure ulcers (PUs) are common skin injuries known for their high morbidity, rapid onset, susceptibility to infection, and challenging healing process. One potential therapy for PUs is cell-based therapy using mesenchymal stem cells (MSCs). However, poor survival and low cell retention of MSCs on skin lesions limit their therapeutic effects and applications. In this study, we prepared an extracellular matrix (dECM) hydrogel decellularized from the human umbilical cord (UC). A patch composed of UC-dECM and UC-MSCs was employed in the treatment of PUs in C57BL/6 mice. Our results indicate that the UC-dECM hydrogel effectively sustains cell viability, enhances the stemness-related gene expression in UC-MSCs, and promotes human umbilical vein endothelial cells (HUVECs) migration and angiogenesis. Compared to the groups treated with the patch containing only UC-dECM, injection of UC-MSCs or gauze dressing, the patch combining UC-dECM hydrogel with UC-MSCs significantly accelerated PU healing. This positive outcome can be attributed to the promotion of tissue re-epithelialization, collagen deposition, angiogenesis, and inflammation inhibition. Our results suggest that the composite patch, comprised of UC-dECM hydrogel and UC-MSCs, may be a promising therapeutic approach for PU treatment.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 433-442"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cochlear implant/MXene-based electroacoustic stimulation modulates the growth and maturation of spiral ganglion neurons","authors":"Yangnan Hu ,&nbsp;Hao Wei ,&nbsp;Menghui Liao ,&nbsp;Shanying Han ,&nbsp;Xin Gao ,&nbsp;Yusong Wang ,&nbsp;Shan Zhou ,&nbsp;Dongyu Xu ,&nbsp;Xugang Zhuang ,&nbsp;Ye Yang ,&nbsp;Hong Cheng ,&nbsp;Bin Zhang ,&nbsp;Qingyue Cui ,&nbsp;Jieyu Qi ,&nbsp;Lei Tian ,&nbsp;Wenyan Li ,&nbsp;Xia Gao ,&nbsp;Renjie Chai","doi":"10.1016/j.engreg.2024.10.001","DOIUrl":"10.1016/j.engreg.2024.10.001","url":null,"abstract":"<div><div>Cochlear implantation (CI) offers a dependable treatment for sensorineural hearing loss, with precision electroacoustic stimulation parameters showing great potential in improving auditory outcomes in CI patients. Here, we report the attachment of MXene into CI systems which effectively mimic the neural electrode interface due to MXene's excellent electrical conductivity and biocompatibility. Low-frequency short-term biphasic electrical pulses emitted by the MXenes-based CI promoted the outgrowth of spiral ganglion neuron (SGN) neurites and growth cones, substantially boosting the calcium activity in SGNs. This study lays a theoretical foundation for the precision medicine approaches in CI patient care, and informs the selection of materials for cochlear implant electrode materials in the future.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 443-451"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing engineered approaches for sustainable wound regeneration and repair: Harnessing the potential of green synthesized silver nanoparticles","authors":"J. Nandhini ,&nbsp;E. Karthikeyan ,&nbsp;E. Elizabeth Rani ,&nbsp;V.S. Karthikha ,&nbsp;D. Sakthi Sanjana ,&nbsp;H. Jeevitha ,&nbsp;S. Rajeshkumar ,&nbsp;Vijayan Venugopal ,&nbsp;A. Priyadharshan","doi":"10.1016/j.engreg.2024.06.004","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.06.004","url":null,"abstract":"<div><p>Wound healing is a crucial biological process for tissue repair and regeneration, preventing infections and complications. There's been a growing interest in exploring sustainable wound healing strategies in recent years. This review examines the use of green-synthesized silver nanoparticles (AgNPs) in sustainable wound healing strategies. It highlights the need for innovative approaches and the challenges posed by infections. The current wound therapies and treatments, highlighting gaps in existing methodologies, are evaluated. This review provides a comprehensive overview of the current state-of-the-art in green synthesis techniques for the synthesis of AgNPs. The properties and characterization of AgNPs are elucidated, providing insights into their efficacy. The biocompatibility of AgNPs in wound healing is also explored, emphasizing safety in medical applications. Green synthesized AgNPs incorporated wound dressings are detailed, showcasing their potential in clinical settings. Challenges and future perspectives are discussed, addressing hurdles to widespread implementation. The conclusion consolidates key findings, offering a synthesized perspective on the potential of green-synthesized AgNPs in revolutionizing current knowledge on innovative approaches for sustainable wound healing practices.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 306-325"},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000379/pdfft?md5=4d51fa5cd78a53e4abd401c3ebcd1952&pid=1-s2.0-S2666138124000379-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141594912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in nano silver-based biomaterials and their biomedical applications","authors":"Punuri Jayasekhar Babu ,&nbsp;Akriti Tirkey ,&nbsp;Abraham Abbey Paul ,&nbsp;Kathelina Kristollari ,&nbsp;Jugal Barman ,&nbsp;Kingshuk Panda ,&nbsp;Neha Sinha ,&nbsp;Birudu Ravi Babu ,&nbsp;Robert S. Marks","doi":"10.1016/j.engreg.2024.07.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.07.001","url":null,"abstract":"<div><p>Silver nanoparticles are among the most widely researched and used for nanotechnology-derived structures due to their extraordinary inherent optical properties, chemical stability, catalytic activity, and high conductivity. These idiosyncratic properties can be attributed to their unique physico-chemical characteristics, such as ultrafine sizes, high surface area, diverse shapes, and strong localized surface plasmon resonance. These distinctive features can be tailored using various physical, chemical, and biological synthesis methods. Various physical techniques are viable for producing silver nanoparticles on a large scale, but they suffer from drawbacks such as high-power consumption, expensive set-up, and limited control over nanoparticle size distribution. Chemical methods provide benefits like high yield, consistent shape and size distribution, and cost efficiency, but the residual toxicity of the chemicals involved hinders their biological applications. Biological synthesis approaches effectively overcome the limitations of both physical and chemical methods by eliminating the need for hazardous chemicals, requiring less energy, enabling diverse nanoparticle morphologies, and offering eco-friendliness and exceptional biocompatibility. The novel and promising properties of nanosilver-based biomaterials have been demonstrated to be suitable for a wide range of pharmacological and therapeutic biomedical applications. Their extensive application in wound healing, dentistry, cardiovascular disease treatment, nerve tissue engineering, cancer treatment, and biosensing can be attributed to their inherent antimicrobial and antibiofilm activity, antithrombotic properties, potential for nerve regeneration, photothermal conversion efficiency and sensitivity, respectively. This review discusses the different methods employed for synthesising silver nanoparticles and focuses on using nanosilver-based biomaterials for various biomedical applications.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 326-341"},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000380/pdfft?md5=03491503b1860689dc45fea734a0f5e4&pid=1-s2.0-S2666138124000380-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141594913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent hydrogels for treating malignant melanoma","authors":"Guopu Chen ,&nbsp;Xiyu Wang ,&nbsp;Jiaye Li ,&nbsp;Ye Xu ,&nbsp;Yue Lin ,&nbsp;Fengyuan Wang","doi":"10.1016/j.engreg.2024.05.004","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.05.004","url":null,"abstract":"<div><p>Malignant melanoma (MM) is an extremely aggressive and fatal form of skin cancer that primarily affects the bottom layer of the epidermis and is associated with poor clinical outcomes. Early-stage MM is typically treated through surgical removal, while chemotherapy and radiotherapy are common conventional treatment options that come with harmful side effects. Emerging therapies such as immunotherapy, photodynamic therapy, biologic therapy, and photothermal therapy present hopeful options for treatment due to their effective and secure drug delivery methods. To address the limitations of current treatment options, advanced methods of drug delivery for subcutaneous MM are being developed, with hydrogels emerging as a promising alternative. To date, significant advancements have been made in the treatment of MM through the use of hydrogels-based drug delivery systems through focal plastering, injection, implantation, and microneedles. Recent research on hydrogel-based drug delivery systems that integrate multiple therapies for the treatment of subcutaneous MM is discussed in this review.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 295-305"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000343/pdfft?md5=be9fd21c46e2f1979b1f682e961bce43&pid=1-s2.0-S2666138124000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rb1 improves human nonalcoholic fatty liver disease with liver organoids-on-a-chip","authors":"Hui Wang ,&nbsp;Yue Zhu ,&nbsp;Pengcheng Shi ,&nbsp;Xiangyang Li ,&nbsp;Qingyun Bu ,&nbsp;Yachun Li ,&nbsp;Xiaoyan You ,&nbsp;Guoping Zhao","doi":"10.1016/j.engreg.2024.06.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2024.06.002","url":null,"abstract":"<div><p>Non-alcoholic fatty liver disease (NAFLD), a type of liver disease for which no treatment is currently approved, remains a major concern worldwide. It is manifested as simple hepatocyte steatosis and can develop into inflammation, fibrosis, cirrhosis and liver cancer in severe cases. However, due to the lack of appropriate <em>in vitro</em> drug testing platforms, an in-depth understanding of the therapeutic activity of ginsenoside Rb₁ in NAFLD remains challenging. Here, we proposed a NAFLD model on a liver organoids (LOs)-on-a-chip platform to evaluate the therapeutic effect of ginsenoside Rb₁ in a dynamic, multi-condition and high-throughput manner. This platform allowed us to reshape certain features such as multicellular types and liver-specific functions of the physiology of the human-relative liver. Free fatty acids (FFAs)-induced LOs displayed typical pathological characteristics of NAFLD progression, including steatosis, oxidative stress, lipid peroxidation, inflammation and fibrosis. With ginsenoside Rb₁ intervention, these pathological features can be significantly improved, which may provide new insights into the potential mechanisms of NAFLD progression and treatment and suggest the clinical implications for humans. The proposed system enables the formation, differentiation, and function of LOs to serve as a scalable, high-throughput and sensitive drug testing model, to potentially expedite the NAFLD drug discovery.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 283-294"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000355/pdfft?md5=9242bd2656ea0d7f1e8b7950d5896503&pid=1-s2.0-S2666138124000355-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding updating Declaration of Competing Interest statements in previously published articles","authors":"","doi":"10.1016/j.engreg.2024.02.003","DOIUrl":"10.1016/j.engreg.2024.02.003","url":null,"abstract":"","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Page 282"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000136/pdfft?md5=2966807c57c23f2f2440ed5819e9bb3d&pid=1-s2.0-S2666138124000136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139891076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of hUC-MSCs conditioned medium on 3D PLLA collagen-coated matrix enhances diabetic wound healing progression","authors":"Siufui Hendrawan ,&nbsp;Olivia Marcelina ,&nbsp;Sukmawati Tansil Tan ,&nbsp;Hans Ulrich Baer","doi":"10.1016/j.engreg.2024.04.005","DOIUrl":"10.1016/j.engreg.2024.04.005","url":null,"abstract":"<div><p>Conditioned medium (CM) derived from human umbilical cord-mesenchymal stem cells (hUC-MSCs) which contains numerous amounts of growth factors, has demonstrated potential in treatment of diabetic wounds. However, for practical application, a biodegradable supporting material is needed to hold the CM and fill in the injury site, where deep cavity wounds are often present in diabetic patients. Poly-<span>l</span>-lactic acid matrix coated with collagen (PLLA/CC) is a suitable carrier due to its biodegradability and biocompatibility. Thus, we present a method to immobilize the hUC-MSCs CM on PLLA/CC through freeze-drying process (PLLA/CC CM FD). When seeded on PLLA/CC CM FD, fibroblasts had an increased cellular function in producing collagen; although no enhancement in cell viability was observed. Moreover, implantation of PLLA/CC CM FD on the wound of diabetic rats showed improvement in wound closure and collagen deposition in the wound area. Altogether, this study exhibits the potential of PLLA/CC CM FD as a therapy for diabetic deep cavity wound.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 3","pages":"Pages 421-431"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000239/pdfft?md5=39d2a2a4e1fdbcf5b4cc3dfedc2e22ca&pid=1-s2.0-S2666138124000239-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141046404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution","authors":"Greg Sacks,&nbsp;Vincent DeStefano,&nbsp;Claire Parker,&nbsp;Ryan Lebens,&nbsp;Harry Mushlin","doi":"10.1016/j.engreg.2024.04.006","DOIUrl":"10.1016/j.engreg.2024.04.006","url":null,"abstract":"<div><p>Nucleus Pulposus (NP) Replacement is a developing surgical methodology for the treatment of pathology related to degeneration of intervertebral discs (IVDs). This article provides necessary context regarding the pathologies treated with this technology, the biomechanical structure and function of the IVD, and the procedures this technology aims to replace. Primarily, it provides an overview and discussion of commercial and experimental preformed and in situ curing prosthesis designs reported in the scientific literature and summarizes the results of biomechanical and clinical studies evaluating their efficacy. Contextual and updated information on the most recent research into NP replacement with novel hydrogel and tissue engineering (TE) strategies is described. Replacement of the NP allows for potential improvement in the treatment of degenerative spinal pathologies through minimally invasive surgical techniques.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 2","pages":"Pages 269-281"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138124000240/pdfft?md5=8b9369e4f3903991157a218650dcbc79&pid=1-s2.0-S2666138124000240-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}