Acta tropica最新文献

{"title":"Serological evidence of Crimean-Congo haemorrhagic fever in domestic animals from eight regions of Namibia","authors":"Lourens de Villiers ,&nbsp;Umberto Molini ,&nbsp;Leandra van Zyl ,&nbsp;Siegfried Khaiseb ,&nbsp;Frank Busch ,&nbsp;Klaas Dietze ,&nbsp;Sascha Knauf ,&nbsp;Giovanni Franzo","doi":"10.1016/j.actatropica.2025.107524","DOIUrl":"10.1016/j.actatropica.2025.107524","url":null,"abstract":"<div><div>Crimean-Congo haemorrhagic fever (CCHF) is a viral zoonotic disease endemic to regions of Africa, the Balkans, the Middle East, and Asia, with increasing reports of cases in southern Europe. Human transmission occurs primarily through the bite of infected ticks and by body fluids from infected human. Crimean-Congo haemorrhagic fever virus (CCHFV) affects a broad host range, including both domestic and wild vertebrates. Recent studies have suggested a potential susceptibility of companion animals, posing an additional threat to public health. In the present study, the presence of CCHFV antibodies was evaluated by screening 374 dog and 238 cat serum samples collected from eight Namibian regions using a commercial enzyme-linked immunosorbent assay. Overall, dogs (11.50 %) showed significantly higher odds of seropositivity (OR = 7.60; 95 % CI = 3.02 - 25.51; <em>p</em>-value &lt; 0.001) compared to cats (1.68 %). Most of the positive dogs and all positive cats originated from the Kunene and Hardap regions. No other factors were found to affect seroprevalence. The limited development of farming systems in these regions, combined with the higher roaming activity of dogs, may suggest a greater exposure risk to wild animals or infected ticks. Tick species of the genus <em>Hyalomma</em> are not commonly reported to infest dogs in Namibia, and other tick species may also be involved in transmission. The detection of CCHFV antibodies in dogs and cats in Namibia should be considered by public health authorities as a potential threat, warranting further investigation to identify infection sources and risk factors. While the level and duration of viremia in companion animals, as well as their actual infectivity, remain unknown, efforts should focus on reducing contact between domestic pets, livestock, and wild animals, as well as regular prophylactic tick treatment of pets to prevent or minimise tick infestations.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107524"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific genetic variation in the lymphatic filariasis vector Mansonia dives (Diptera: Culicidae) in Thailand: Hidden species or genetically divergent populations?","authors":"Sedthapong Laojun ,&nbsp;Tanasak Changbunjong ,&nbsp;Tanawat Chaiphongpachara","doi":"10.1016/j.actatropica.2025.107526","DOIUrl":"10.1016/j.actatropica.2025.107526","url":null,"abstract":"<div><div><em>Mansonia dives</em> is recognized as a vector for brugian filariasis in Thailand. A recent study analyzing the cytochrome <em>c</em> oxidase subunit I (<em>COI</em>) gene revealed two distinct clades within the <em>Ma. dives</em> population in Thailand. This study aimed to examine the genetic diversity and structure of <em>Ma. dives</em> using the <em>COI</em> gene and the internal transcribed spacer 2 (ITS2) region to determine the presence of distinct species or genetically divergent populations. We analyzed 60 <em>COI</em> and 60 ITS2 sequences from <em>Ma. dives</em> populations in Narathiwat, Ranong, Tak, and Trat. The results showed a nucleotide diversity of 0.019 and a haplotype diversity of 0.979 for the <em>COI</em> gene, while the ITS2 region displayed a nucleotide diversity of 0.005 and a haplotype diversity of 0.545. Phylogenetic and haplotype network analyses of the <em>COI</em> gene identified two genetic lineages: one confined to Trat and another encompassing the other sites. However, species delimitation methods suggested that these genetic differences were insufficient to classify the lineages as distinct species. In contrast, the ITS2 analysis indicated a uniform genetic pattern across all populations. We conducted neutrality tests and mismatch distribution to examine the demographic history. For the <em>COI</em> gene, Tajima's <em>D</em> was slightly positive and non-significant (0.014), while Fu's <em>Fs</em> was negative (−9.750), indicating a potential expansion phase. Conversely, for the ITS2 region, Tajima's <em>D</em> and Fu's <em>Fs</em> were positive and non-significant, suggesting that the population might be in equilibrium or undergoing contraction. Moreover, the mismatch distribution analysis for the ITS2 region was inconclusive. The apparent discrepancies between these markers indicate the presence of genetically divergent populations, rather than distinct species.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107526"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odor masking to decrease the attraction of Triatoma infestans (Hemiptera: Reduviidae, Triatominae), a Chagas disease vector, towards a host","authors":"Andrea Martínez Beningaza ,&nbsp;I.M. Desiree Di Benedetto ,&nbsp;Lucía Babino ,&nbsp;Pablo G Guerenstein","doi":"10.1016/j.actatropica.2024.107520","DOIUrl":"10.1016/j.actatropica.2024.107520","url":null,"abstract":"<div><div>Chagas disease, vectored by kissing bugs, poses a public health problem across the Americas. The best way for reducing disease transmission is through vector control, which is currently based on the use of insecticides. However, insecticide resistance, and environmental and health issues, stress the need for new, environmentally-friendly methods for reducing vector-host contacts. Kissing bugs use the odor blend emitted by hosts to locate them. The proportions of the constituents of that blend are important to determine the attraction evoked by it. We aimed at assessing whether artificial alterations of those proportions could render an attractive host, unattractive for the kissing bug <em>Triatoma infestans</em>. Thus, the behavior of the bugs after adding a source of nonanal, a component of vertebrate odor detected by the bugs, to an attractive mouse was studied. For this, nymphs were tested using a dual-choice trap-olfactometer and different doses of nonanal. Addition of nonanal resulted in a dose- dependent reduction in the attraction of the bugs towards the mouse although it did not prevent the bugs from leaving their release zone nor it affected their (reduced) host preference near the host. Nonanal <em>per se</em> did not evoke any behavior at the doses tested. We suggest that the increase in the proportion of nonanal resulted in masking of the mouse odor. This approach could help reach a “push” effect within an effective push- pull strategy for kissing bugs. That strategy would help diminish the vector-host contacts and, therefore, Chagas disease incidence, in an environmentally-friendly manner. It remains to be established whether this strategy proves effective in the field when adequate odor dispensers and socially acceptable odors are used.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107520"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and serological investigation of Brucella species in kennel and farm dogs in Iran","authors":"Baharak Akhtardanesh ,&nbsp;Elham Mohammadi ,&nbsp;Soheil Sadr ,&nbsp;Asma Askari ,&nbsp;Zeinab Manzari Tavakoli ,&nbsp;Rozhin Ahmadi ,&nbsp;Shakiba Nazemian ,&nbsp;Hossein Rashidi ,&nbsp;Morteza Aghamiri ,&nbsp;Mehdi Golchin ,&nbsp;Masoud Imani","doi":"10.1016/j.actatropica.2025.107521","DOIUrl":"10.1016/j.actatropica.2025.107521","url":null,"abstract":"<div><h3>Introduction</h3><div>Brucellosis is still a significant emerging threat to public health, as it can infect humans, wild, domestic animals, and livestock. Hence, the current study aims to determine the frequency of canine brucellosis (CB), its relationship with clinical findings and reproductive disorders in kennel and farm dogs, and its importance on public health.</div></div><div><h3>Materials and methods</h3><div>From January 2022 to December 2023, a total of 150 blood samples were taken from 100 adult dogs in breeding kennels and 50 shepherd dogs in breeding farms in Kerman, Iran. Rose Bengal test (RBT) and Wright tests were used for the primary screening of <em>Brucella abortus</em> (<em>B. abortus</em>) and <em>Brucella melitensis</em> (<em>B. melitensis</em>) species, and conventional IS711-based PCR and real-time polymerase chain reaction (RT-PCR) were performed to determine the <em>Brucella</em> genus and species in all samples.</div></div><div><h3>Findings</h3><div>In kennel dogs, 34 % (34/100) had a history of breeding disorders, and 30 % (30/100) were RBT-positive. Moreover, <em>B. canis</em> and <em>B. abortus</em> species were detected in kennel dogs. Among farm dogs, 16 % (8/50) had a history of breeding disorders, and 28 % (14/50) were RBT positive. Additionally, <em>B. canis, B. abortus</em>, and <em>B. melitensis</em> species were detected in farm dogs. Feeding with unpasteurized milk was significantly related to a positive RBT in kennel dogs (<em>p</em> = 0.009), and there was a significant correlation between breeding disorders and seropositivity in kennel (<em>p</em> = 0.045) and farm dogs (<em>p</em> = 0.03).</div></div><div><h3>Conclusion</h3><div>The current study represents a significant advancement in understanding CB in Iran by the first molecular detection of <em>B. canis</em>, revealing <em>B. melitensis</em> and <em>B. abortus</em> as important pathogens in kennel and farm dogs and highlighting the public health significance of disease in Iran.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107521"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In search of pathogenic Leptospira species in Myotis and other vesper bats, Russia","authors":"Alex Lintu Viskontene ,&nbsp;Ekaterina V. Radyuk ,&nbsp;Oleg A. Shapkin ,&nbsp;Evgeniy A. Khizhkin ,&nbsp;Victoria P. Bulanenko ,&nbsp;Yana A. Voytsekhovskaya ,&nbsp;Sergey G. Medvedev ,&nbsp;Lyudmila S. Karan","doi":"10.1016/j.actatropica.2024.107506","DOIUrl":"10.1016/j.actatropica.2024.107506","url":null,"abstract":"<div><div>Various bat species worldwide have been identified as <em>Leptospira</em> carriers, especially in tropical regions. In this study, we investigated the infection of Vespertilionidae bats by pathogenic <em>Leptospira</em> in north-west Russia. Out of 264 bats from 13 species, the urine of 24 specimens tested positive according to a polymerase chain reaction test. The infected species were exclusively <em>Myotis</em> bats: <em>M. brandtii</em> (1/56; 1.8 %); <em>M. dasycneme</em> (9/40; 22.5 %); and <em>M. daubentonii</em> (14/47; 29.8 %). The detected <em>Leptospira</em> strains were similar to <em>L. kirschneri</em> and <em>L. borgpetersenii</em>.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107506"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wolbachia-based mosquito control: Environmental perspectives on population suppression and replacement strategies","authors":"Joydeb Bhattacharyya ,&nbsp;Daniel L. Roelke","doi":"10.1016/j.actatropica.2024.107517","DOIUrl":"10.1016/j.actatropica.2024.107517","url":null,"abstract":"<div><div>Mosquito-borne diseases pose a significant threat to global health, and traditional mosquito control methods often fall short of effectiveness. A promising alternative is the biological control strategy of transinfecting mosquitoes with <em>Wolbachia</em>, a bacterium capable of outcompeting harmful pathogens and reducing the ability of mosquitoes to transmit diseases. However, <em>Wolbachia</em> infections are sensitive to abiotic environmental factors such as temperature and humidity, which can affect their densities in mosquitoes and, consequently, their ability to block pathogens. This review evaluates the effectiveness of different <em>Wolbachia</em> strains transinfected into mosquitoes in reducing mosquito-borne diseases. It explores how <em>Wolbachia</em> contributes to mosquito population control and pathogen interference, highlighting the importance of mathematical models in understanding <em>Wolbachia</em> transmission dynamics. Additionally, the review addresses the potential impact on arboviral transmission and the challenges posed by environmental fluctuations in mosquito control programs.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107517"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and prevalence of Blastocystis subtypes in Alborz Province, Iran: A molecular epidemiological study","authors":"Ehsan Karimi ,&nbsp;Zohreh Momeni ,&nbsp;Vahid Nasiri ,&nbsp;Azar Sabokbar","doi":"10.1016/j.actatropica.2025.107537","DOIUrl":"10.1016/j.actatropica.2025.107537","url":null,"abstract":"<div><div>The primary objective of this study was to evaluate the genetic diversity of <em>Blastocystis</em>. Additionally, it aimed to explore, for the first time in Iran, the potential association between <em>Blastocystis</em> infection and ABO blood groups. Another focus was to examine the relationship between <em>Blastocystis</em> subtypes and blood groups, an often overlooked risk factor, within the population of Alborz Province, Iran. 450 stool samples were collected from diagnostic facilities across Alborz Province between June 2022 and April 2024. The presence of <em>Blastocystis</em> was confirmed in 64 out of 450 samples (14.22 %) using the Nested PCR-RFLP technique. Among the 64 positive samples, nine (2 %) were classified as indeterminate.</div><div>Two distinct <em>Blastocystis</em> subtypes were identified in 55 isolates, accounting for 12.22 % of the total samples. ST1 was detected in 44 isolates (9.77 %), rendering it the most prevalent subtype, while ST3 was identified in 11 isolates (2.44 %). Phylogenetic trees were constructed using sequencing data and compared against genotypes available in GenBank. Significant differences between infected and non-infected individuals were observed regarding educational attainment, marital status, and blood type (<em>p</em> &lt; 0.05). A significant association was found between ABO blood groups and the prevalence of <em>Blastocystis</em> infection (<em>p</em> = 0.019). However, when examining the correlation between <em>Blastocystis</em> subtypes (ST1 and ST3) and ABO blood groups, no significant overall association was observed. Nonetheless, specific associations were found for blood groups O+ and B-, with subtype ST1 being more prevalent in these groups.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107537"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new myxozoan parasites, Myxobolus mayarum n. sp. and Kudoa mayarum n. sp., infecting the neotropical fish Mayan Cichlid, Mayaheros urophthalmus (Günther, 1862) in the Yucatán Peninsula, Mexico","authors":"Graciela Colunga-Ramírez ,&nbsp;M. Leopoldina Aguirre-Macedo ,&nbsp;Kálmán Molnár ,&nbsp;Csaba Székely ,&nbsp;Boglárka Sellyei ,&nbsp;Gábor Cech","doi":"10.1016/j.actatropica.2025.107527","DOIUrl":"10.1016/j.actatropica.2025.107527","url":null,"abstract":"<div><div>The biodiversity of freshwater fishes is extensive in Mexico; however, knowledge of their associated myxozoan parasites is limited. This study aimed to recognize myxozoan parasites in the endemic fish <em>Mayaheros urophthalmus</em>. Two new species, <em>Myxobolus mayarum</em> n. sp. and <em>Kudoa mayarum</em> n. sp. were described from <em>M. urophthalmus</em> collected in the Celestún Coastal Lagoon and Baldiosera Freshwater Spring from the Yucatán Peninsula. <em>Myxobolus mayarum</em> n. sp. was found in 100 % of the examined fish, infecting the gills, kidney, pectoral fins, and spleen. The spores were round, 11.34 ± 0.67 μm length, 10.03 ± 0.44 μm width, and 7.22 ± 0.45 μm thickness. The polar capsules were pyriform and equal in size, 3.85 ± 0.29 μm length and 2.27 ± 0.20 μm width. The polar tubule 30.71 ± 2.10 μm long and coiled 5–6 times. <em>Kudoa mayarum</em> n. sp. was found in 30 % of fish, infecting the heart, oesophagus, and stomach. The spores were subspherical, 5.40 ± 0.40 μm length and 5.76 ± 0.37 μm width. The polar capsules were ellipsoidal and equal in size, 1.90 ± 0.27 μm length and 1.46 ± 0.20 μm width. The phylogenetic analyses based on the small and large subunits ribosomal DNA (18S rDNA and 28S rDNA) sequences demonstrated that these two species are distinct from other published myxozoans, providing evidence to support the description of two new species. This study constitutes the first record of myxozoans from the Yucatán Peninsula, contributing to increase the knowledge about the biodiversity of myxozoans in neotropical regions.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107527"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs","authors":"Lucía M. Campero ,&nbsp;Ignacio Gual ,&nbsp;Valeria A. Sander ,&nbsp;Luisa F. Mendoza Morales ,&nbsp;Victor A. Ramos Duarte ,&nbsp;Paula M. Formigo ,&nbsp;Emiliano Sosa ,&nbsp;Fermín Lázaro ,&nbsp;María Valeria Scioli ,&nbsp;Agustín Atela ,&nbsp;Ariel Legarralde ,&nbsp;Federico A. Hozbor ,&nbsp;Germán J. Cantón ,&nbsp;Sergio O. Angel ,&nbsp;Dadín P. Moore ,&nbsp;Marina Clemente","doi":"10.1016/j.actatropica.2025.107540","DOIUrl":"10.1016/j.actatropica.2025.107540","url":null,"abstract":"<div><div><em>Toxoplasma gondii</em> is a protozoan parasite causing toxoplasmosis, a principal concern for public health and livestock industries. Effective vaccination strategies are crucial for controlling toxoplasmosis, particularly in the lamb, which are significant reservoirs of <em>T. gondii</em>. In addition, ovine toxoplasmosis also causes economic losses due to abortions and reproductive complications. In this study, we evaluated two immunization strategies to elucidate the immune protective potential of <em>T. gondi</em> major surface protein SAG1 fused to the plant heat shock proteins 90-kDa (pHsp90) adjuvant against experimental toxoplasmosis in lambs. We performed an oral administration of fresh leaves homogenate infiltrated with a B- and T-cell antigenic epitope-containing surface protein SAG1 (SAG1HC) fused to <em>Arabidopsis thaliana</em> Hsp90 (AtHsp81.2-SAG1HC) (Plant Vaccine) and a subcutaneous administration of recombinant SAG1HC fused to <em>Nicotiana benthamiana</em> Hsp90 (NbHsp90.3-SAG1HC) produced in <em>Escherichia coli</em> (Recombinant Vaccine). Our results showed that only the Recombinant Vaccine significantly increased anti-rSAG1 total IgG values (∼ 4-fold more than the Vehicle and Control groups). In addition, only lambs immunized with the Plant Vaccine showed a significant increase (∼ 3-fold more than the Vehicle and Control groups) in IFN-γ serum levels after the experimental infection (evaluated 8 days post-challenge). On the other hand, we also observed a statistically significant decrease (∼ 80 % less) in histopathological lesions (injury score) in challenged vaccinated lambs compared to challenged but not vaccinated animals (Vehicle and Control groups). Previously, we showed that the chimera recombinant Gra4-Gra7 protein is an acute marker of human infection. Since Gra4-Gra7 is not connected to the SAG1 immunogen, this chimera allows us to monitor infection in challenged lambs early. All lambs from the Control and Vehicle groups showed higher rates of serological reactivity than lambs from the vaccinated groups, concurrently with increased severity of lesions. These results suggest that the Plant-based and Recombinant Vaccines are promising candidates for controlling <em>T. gondii</em> infection in lambs, with potential benefits for enhancing public health and animal welfare.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"262 ","pages":"Article 107540"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential B cell mediated immune response during Plasmodium yoelii infection in mice","authors":"Meenu Kalkal,&nbsp;Jyoti Das","doi":"10.1016/j.actatropica.2025.107533","DOIUrl":"10.1016/j.actatropica.2025.107533","url":null,"abstract":"<div><div>B lymphocytes are essential components of the humoral immune response and categorized into various subsets according to specific surface markers, functions, and developmental stages. Each subset of B cells plays a distinct role in the immune response, contributing to the overall effectiveness of the immune system. In this study, we investigated the modulation of different splenic subsets of B cells during <em>Plasmodium yoelii</em> infection. Balb/c mice infected with each <em>Plasmodium yoelii</em> XL and <em>Plasmodium yoelii</em> XNL parasite were used for phenotypic characterization of splenic B cell subsets through flow-cytometry. Our findings indicate that both lethal and non-lethal infections of <em>Plasmodium yoelii</em> result in significant alterations within the B cell compartment of the spleen in Balb/c mice during malaria infection. Notably, a differential expansion of immature B cell subsets T1 and T2 was noticed. A continuous reduction in frequency of both subsets (T1 and T2) during infection with lethal parasite while an increase in these subsets during the recovery from infection with non-lethal parasite was observed. Further, the frequencies of mature B cell subsets, follicular B cells and marginal zone B cells, were reduced during lethal infection which may be leading to susceptibility. Whereas non-lethal parasite infection resulted in increased frequency of follicular B cells in spleen which indicates towards establishment of germinal centre for generation of long-term immunity/resistance to infection. This differential expansion of splenic B cell subsets reflects the distinct characteristics of lethal and non-lethal parasite. Overall, these findings illustrate the potential role of B cells in resistance/susceptibility during malaria infection and further enhance our understanding of the B cell mediated immunological aspects of <em>Plasmodium</em> infection.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107533"},"PeriodicalIF":2.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}