In vivo evaluation of three isoxazolines against Cx. tritaeniorhynchus (Diptera: Culicidae): A novel approach to control Japanese encephalitis vector

As Culex tritaeniorhynchus the primary vector of JE is a highly zoophagic and exophilic nature, a novel vector control approach use of isoxazolines drugs (Sarolaner, Fluralaner and Afoxolane) to reduce the survival of blood-fed mosquito was studied by in vivo model using Rattus norvegicus as an experimental animal. All the drugs are orally administered to the animal with therapeutic dosage. The drugs Fluralaner, Sarolaner and Afoxolaner induced 100 % mortality of Cx. tritaeniorhynchus up to 32, 7 and 20 days respectively after administered to the R. norvegicus. When Cx. tritaeniorhynchus was fed on animals treated with Fluralaner, the median survival duration was 12 h from days 1 to 7 of post-treatment and 24 h from days 8 to 28.