Morphological and molecular characterization of Triatominae bugs collected in Sonora, Mexico

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-05-15 DOI:10.1016/j.actatropica.2025.107654

Olivia Valenzuela , Lesley Bogarin , Idalia Paredes , Mónica Resendiz-Sandoval , Judith Valdez , Heriberto Torres , Sebastián Pita , Joel Moo-Millan , Etienne Waleckx

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引用次数: 0

Abstract

Triatomines (Hemiptera: Reduviidae: Triatominae) are vectors of Trypanosoma cruzi, the causative agent of Chagas disease. This study aimed to assess the diversity of triatomines collected in 2023 from various regions of the State of Sonora, in Northwestern Mexico, from both wild (mainly rodent nests) and urban sites. Overall, 75 wild nests were dissected, with 36 % (27/75) positive for triatomines. A total of 180 triatomine specimens were collected, including 170 collected in wild nests, and ten collected in human dwellings. Identification was performed using taxonomic keys (for adults only) as well as sequencing of a fragment of the nuclear internal transcribed spacer 2 (ITS-2) (for both adults and nymphs). Our results confirmed the presence of at least four triatomine species: Hospesneotomae sinaloensis (formerly Triatoma sinaloensis), Triatoma rubida, Hospesneotomae protracta (formerly Triatoma protracta), and Triatoma recurva. Notably, T. rubida and H. protracta (T. protracta), on the one hand, as well as T. rubida and H. sinaloensis (T. sinaloensis) on the other hand, were frequently found cohabiting in the same nest. Furthermore, this work provides the first ITS-2 molecular characterization of H. sinaloensis (T. sinaloensis), and its phylogenetic relationships with other members of the Triatomini tribe. Divergent haplotypes were found, that could be related to the presence of cryptic species in our study area.

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墨西哥索诺拉地区三角蝽科昆虫的形态和分子特征。

锥虫（半翅目：锥虫科：锥虫科）是恰加斯病病原体克氏锥虫的媒介。本研究旨在评估2023年在墨西哥西北部索诺拉州不同地区（主要是啮齿动物巢穴）和城市地点收集的三爪鼠的多样性。总的来说，75个野生鸟巢被解剖，36%（27/75）的三聚氰胺阳性。共采集了180只三角蝽标本，其中野生巢采集170只，人类住所采集10只。鉴定使用分类键（仅适用于成虫）以及核内部转录间隔段2 （ITS-2）片段测序（适用于成虫和稚虫）。我们的研究结果证实了至少四种三角蝽的存在：sinaloensis Hospesneotomae（原Triatoma sinaloensis）， T. rubida, Hospesneotomae protracta（原Triatoma protracta）和T. recurva。值得注意的是，在同一巢穴中，rubida和H. protracta （T. protracta）以及T. sinaloensis （T. sinaloensis）经常被发现在同一巢穴中同居。此外，本研究首次提供了H. sinaloensis （T. sinaloensis）的its -2分子特征，以及它与Triatomini部落其他成员的系统发育关系。发现了不同的单倍型，这可能与我们研究区存在隐种有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.