Acta tropica最新文献

{"title":"Eco-epidemiological Insights into Psammolestes arthuri: Trypanosoma cruzi Infection and Feeding Behavior in Casanare, Colombia","authors":"Tatiana M. Cáceres ,&nbsp;Carolina Hernandez ,&nbsp;Luz Helena Patiño ,&nbsp;Plutarco Urbano ,&nbsp;Karen Barragan ,&nbsp;Efren Ponare ,&nbsp;Juan David Ramírez","doi":"10.1016/j.actatropica.2025.107790","DOIUrl":"10.1016/j.actatropica.2025.107790","url":null,"abstract":"<div><div>Chagas disease, caused by the protozoan parasite <em>Trypanosoma cruzi</em>, affects millions of people and is primarily transmitted by triatomine bugs. Although <em>Psammolestes arthuri</em> has been traditionally classified as a secondary vector due to its predominantly ornithophilic feeding habits, recent findings suggest that its ecological and behavioral traits may enhance its epidemiological relevance. We hypothesized that <em>P. arthuri</em> exhibits natural infection rates, flexible feeding behavior with frequent mammalian contacts, and spatial variation in parasite load that challenge its conventional classification. To test this, we assessed <em>T. cruzi</em> infection prevalence, parasite load, and blood meal sources in 61 specimens collected from two municipalities in Casanare, eastern Colombia—San Luis de Palenque (<em>n</em> = 43) and Tauramena (<em>n</em> = 18)—between April and October 2023. Infection was determined by qPCR, and feeding sources were identified by 12S rRNA gene sequencing. The results revealed a 68.8 % infection rate with a mean parasite load of 70.40 parasites/mL, with adult males showing the highest loads. Feeding analysis indicated a predominance of <em>Anhinga anhinga</em> (64.9 %), followed by <em>Phalacrocorax brasilianus</em> (6.8 %), <em>Homo sapiens</em>, and other species. The detection of human blood, particularly in nymphs, suggests behavioral adaptation toward opportunistic feeding in proximity to human dwellings. These findings support the hypothesis that <em>P. arthuri</em> may contribute to local transmission dynamics of <em>T. cruzi</em> and highlight the need to consider both avian and mammalian hosts in Chagas disease surveillance.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107790"},"PeriodicalIF":2.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metatranscriptomic profiling of the bacterial and fungal microbiota of field-collected Anopheles darlingi from Colombia: Community composition and functional insights","authors":"Paola Muñoz-Laiton ,&nbsp;Juan C. Hernandez-Valencia ,&nbsp;Juan Pablo Isaza ,&nbsp;Margarita M. Correa","doi":"10.1016/j.actatropica.2025.107795","DOIUrl":"10.1016/j.actatropica.2025.107795","url":null,"abstract":"<div><div>Mosquitoes harbor diverse microorganisms that can influence host biology. While microbiota research in <em>Anopheles</em> has primarily focused on the metabarcoding approach, knowledge regarding the transcriptionally active microbiota and its functional roles in the host remains limited. This study aimed to characterize the transcriptionally active bacterial and fungal microbiota in field-collected <em>Anopheles darlingi</em>, a primary malaria vector in the Neotropics, and in addition, to explore its functional profile. Mosquitoes were collected in malaria-endemic regions of Colombia. Total RNA was extracted from pooled specimens to prepare cDNA libraries and sequenced by Illumina NovaSeq 6000. Results on microbiota composition showed predominance of bacterial (65 %) over fungal reads (35 %). The most abundant bacterial families included Enterobacteriaceae, Pseudomonadaceae, Acetobacteraceae and Moraxellaceae; the dominant genera were <em>Halopseudomonas, Acinetobacter, Klebsiella, Escherichia</em> and <em>Asaia</em>. In the fungal microbiota composition predominated Aspergillaceae and Hypoxylaceae, primarily represented by <em>Aspergillus</em> and <em>Hypoxylon</em>. Functional analysis showed transcripts encoding an MFS family transporter - an arabinose efflux permease, an ABC-type branched-chain amino acid transport system, diaminopimelate decarboxylase and O-succinylbenzoic acid-CoA ligase (MenE). These transcripts were associated with functional categories related to carbohydrate transport and metabolism, as well as the transport and biosynthesis of essential amino acids and vitamins. These findings offer valuable insights into the microbiota-mosquito dynamic and establish a foundation for further research into the role of microbiota in mosquito biology.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107795"},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First experimental evidence of the transovarial transmission of lumpy skin disease virus by Aedes aegypti (Diptera: Culicidae)","authors":"Chalida Sri-in ,&nbsp;Aunyaratana Thontiravong ,&nbsp;Patchana Kamkong ,&nbsp;Tapanut Songkasupa ,&nbsp;Lyric C. Bartholomay ,&nbsp;Sonthaya Tiawsirisup","doi":"10.1016/j.actatropica.2025.107794","DOIUrl":"10.1016/j.actatropica.2025.107794","url":null,"abstract":"<div><div>Lumpy skin disease virus (LSDV), which belongs to the family Poxviridae, causes severe illness in livestock. Although the exact mechanisms of LSDV transmission remain unclear, it is thought to spread through various routes, including direct and indirect contact, as well as an arthropod-borne viral disease. Although biological vectors are considered the most effective virus reservoirs, few studies have explored potential LSDV vectors. <em>Aedes</em> (<em>Ae.</em>) <em>aegypti</em> is a well-known vector of several significant diseases, including lumpy skin disease, which is known to cause widespread infections in animals and livestock each year. Therefore, understanding the interaction between LSDV and mosquito vectors can lead to the development of alternative strategies for controlling LSDV outbreaks naturally by blocking virus transmission within its vectors. This study aimed to investigate the potential for the transovarial transmission of LSDV by <em>Ae. aegypti</em>. Viral infection in the subsequent generation of mosquitoes was evaluated. This research is expected to clarify the role of <em>Ae. aegypti</em> in LSDV viability and assess the potential for transovarial transmission in mosquito vectors. The findings indicated that <em>Ae. aegypti</em> is a potential biological vector of LSDV. This is the first study to demonstrate the potential transovarial transmission of LSDV in <em>Ae. aegypti</em> when infected with a sufficiently high viral load at an appropriate time.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107794"},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in recombinant protein vaccines against Leishmania infantum: a systematic review of vaccinal constructs and delivery strategies","authors":"André Zaidan Martins ,&nbsp;Ana Laura Grossi de Oliveira ,&nbsp;Lilian Lacerda Bueno ,&nbsp;Fabrício Marcus Silva Oliveira ,&nbsp;Ricardo Toshio Fujiwara","doi":"10.1016/j.actatropica.2025.107796","DOIUrl":"10.1016/j.actatropica.2025.107796","url":null,"abstract":"<div><div>Leishmaniasis remains one of the most prevalent neglected tropical diseases, with <em>Leishmania infantum</em> being a major cause of visceral leishmaniasis, a potentially fatal condition if left untreated. Despite advances in vaccine development, no human vaccine is currently licensed, highlighting the urgent need for innovative immunization strategies. Recombinant protein-based vaccines have emerged as promising candidates due to their capacity to induce targeted immune responses. This review synthesizes findings from 30 studies, identified through a systematic search in PubMed, Scopus, and Web of Science (January 2000 to February 2025) following PRISMA guidelines, evaluating recombinant protein vaccines in Leishmania infantum-infected BALB/c mice, with emphasis on vaccine formulations, delivery methods, and immunological outcomes. Most studies employed subcutaneous immunization and saponin-based adjuvants, testing a variety of antigens, including full-length proteins, chimeric constructs, and unique epitopes. Consistently, these vaccines elicited a Th1-skewed immune response marked by elevated IFN-γ, IL-12, and GM-CSF levels, along with a high IgG2a/IgG1 ratio, immunological indicators of effective protection. Significant reductions in parasitic load were observed in key organs such as the liver, spleen, and bone marrow across vaccinated groups. While all constructs demonstrated protective efficacy, none showed clear superiority. Although modern delivery platforms like nanoparticles, liposomes, and polymer-based systems have shown promise in enhancing antigen stability and immunogenicity, their exploration remains limited, signaling a critical research gap. To advance the development of recombinant protein vaccines against <em>Leishmania infantum</em>, future efforts should prioritize the discovery of novel antigens, optimization of adjuvant formulations, and integration of innovative delivery platforms.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107796"},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrogenome analysis of rodents from Heilongjiang Province, China","authors":"Qing Liu ,&nbsp;Ming Wang ,&nbsp;Jing-Wen Xie ,&nbsp;Yu-Tong Du ,&nbsp;Zi-Ge Yin ,&nbsp;Jing-Hong Cai ,&nbsp;Ming-Hui Zhao ,&nbsp;Yu-Ting Jiang ,&nbsp;Heng-Duan Zhang","doi":"10.1016/j.actatropica.2025.107789","DOIUrl":"10.1016/j.actatropica.2025.107789","url":null,"abstract":"<div><div>Rodents are carriers and hosts of numerous zoonotic pathogens, analysing the diversity of viruses and bacteria carried by rodents is important for predicting and reducing the risk of future outbreaks of zoonotic diseases. Heilongjiang is a border province in China with rich ecological resources. In this study, we characterized the zoonotic microbiota (viruses and bacteria) in wild rodents. Sixty-seven rodents representing two species (20 <em>Apodemus peninsulae</em>; 47 <em>Myodes rufocanus</em>) were collected in Hengdaohezi Town, Heilongjiang Province, China. Metagenomic sequencing was employed to characterize pathogen carriage in these reservoirs. The sequencing results showed that the annotated viruses covered 21 viral families, including family <em>Arenaviridae</em> and family <em>Hantaviridae</em>. The annotated bacteria cover 1051 bacterial genera, including genus <em>Salmonella</em> and genus <em>Yersinia</em>. Results of de novo assembly of extracted viral sequences using Megahit showed that 154 contigs were assembled from these two hosts, and 110 of these contigs were mapped to 19 viruses from eight families, including Rat arterivirus1, Amur virus and Lassa mammarenavirus, these results can provide a certain reference for the monitoring and control of rodents in Heilongjiang Province.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107789"},"PeriodicalIF":2.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal dynamics of deltamethrin resistance in Triatoma infestans: Challenges for chagas disease vector control in Argentina","authors":"Patricia A. Lobbia ,&nbsp;Carolina Remón ,&nbsp;Agustina Tello ,&nbsp;Alasco López ,&nbsp;Leonardo Iula ,&nbsp;Martín Amigo ,&nbsp;Ana Laura Carbajal-de-la-Fuente ,&nbsp;Mariana Manteca-Acosta","doi":"10.1016/j.actatropica.2025.107791","DOIUrl":"10.1016/j.actatropica.2025.107791","url":null,"abstract":"<div><div>The emergence of insecticide resistance in <em>Triatoma infestans</em>, driven by extensive pyrethroid use, represents a major obstacle to effective Chagas disease vector control in Argentina. Given the spread of resistance and the impact on control efforts, understanding its geographical and temporal patterns is crucial for guiding resilient and effective intervention efforts. This study presents a spatio-temporal analysis of deltamethrin resistance across nine Argentine provinces during 2013–2023 period, based on biotoxicological assays of <em>T. infestans</em> populations collected from 155 locations, complemented by historical resistance data from the literature and analysis of insecticide application. Resistance levels were classified as low, medium, or high based on resistance ratios calculated from DL₅₀ values. Results show that 46.45 % of the populations analyzed were resistant to deltamethrin, with resistant populations found in five provinces, indicating a notable geographic expansion into new ecoregions. We report the first widespread emergence of medium resistance levels (10 &lt; RR₅₀ &lt; 100) in four provinces, alongside geographic expansion into new ecoregions, highlighting an active process of resistance evolution with notable regional variability. Combining resistance data with insecticide application we observed three distinct resistance patterns in vector populations: stable susceptible, stable resistance, and variable resistance levels over time. Our findings suggest that conventional pyrethroid treatments may be insufficient, especially in high-resistance regions. This study underscores the need for adaptive, region-specific management approaches and the integration of continuous monitoring efforts, as initiated by Argentina’s National Pesticide Resistance Surveillance Network (RAReP). These efforts are essential for enhancing vector control efficacy and mitigating the spread of Chagas disease.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107791"},"PeriodicalIF":2.5,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal activity of purified compounds and their derivatives from Ocimum americanum L. against dengue virus vector Aedes aegypti","authors":"Ramachandran Maduraiveeran ,&nbsp;Balakrishna Kedike ,&nbsp;Shakila Ramachandran ,&nbsp;Jayakumar Manickkam","doi":"10.1016/j.actatropica.2025.107793","DOIUrl":"10.1016/j.actatropica.2025.107793","url":null,"abstract":"<div><div><em>Ocimum americanum</em> is commonly known as hoary basil, wild basil and American basil. It is used in traditional medicine for the treatment of microbial infections, fever, cold, tuberculosis and constipation. The insecticidal activity in <em>Ocimum americanum</em> is very limited. The aim of this study is to isolate the effective mosquitocidal compounds against <em>Aedes aegypti</em>. To isolate the bioassay-guided mosquitocidal compounds and their derivatives against <em>Aedes aegypti</em> larvae. The successive extracts of the aerial parts of <em>Ocimum americanum</em> (Lamiaceae), were tested against the third instar larvae of <em>Aedes aegypti</em>. Bioactivity guided fractionation of the most active extract gave the active constituents. Further, activities of the derivatives, viz.<em>,</em> acetate and propionates, were also investigated. Of the successive extracts of the aerial parts of the plant, viz.<em>,</em> hexane, chloroform and methanol, the chloroform extract showed the highest activity. The LC₅₀ and LC₉₀ values after 48 h were 337.94 and 918.79 ppm, respectively. Bioassay-guided fractionation of the active chloroform extract resulted in the isolation of the active principles viz.<em>,</em> oleanolic acid and ursolic acid. The LC₅₀ and LC₉₀ values of the compounds were 42.41, 78.39 and 26.09, 62.06 ppm, respectively. In the case of the acetate derivative, both compounds showed higher activity; among them, oleanolic acid acetate showed the highest activity; LC₅₀ and LC₉₀ values were 4.97 and 9.31 ppm, respectively. The pentacyclic triterpenoids oleanolic acid and ursolic acid were identified as the active larvicidal constituents against the third instar larvae of <em>Aedes aegypti</em>. Oleanolic acid acetate being the most active derivative.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107793"},"PeriodicalIF":2.5,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Access to water, sanitation, and hygiene (WASH) facilities and key influencing factors in coastal households, Bangladesh","authors":"Shuvagato Mondal ,&nbsp;Kinley Wangdi ,&nbsp;Darren James Gray ,&nbsp;Matthew Kelly ,&nbsp;Haribondhu Sarma","doi":"10.1016/j.actatropica.2025.107792","DOIUrl":"10.1016/j.actatropica.2025.107792","url":null,"abstract":"<div><div>This study assessed the accessibility and determinants of water, sanitation, and hygiene (WASH) facilities in Bangladesh’s climate-sensitive coastal region, which is highly vulnerable to sea-level rise, salinity intrusion and extreme weather events. A cross-sectional survey was conducted from March to July 2023, involving 471 households across nine subdistricts within three coastal zones using a three-stage cluster sampling technique. Multivariable logistic regression was performed to identify the associated factors, considering WASH indicators (as defined by the WHO/UNICEF Joint Monitoring Program) as outcomes and sociodemographic characteristics as exposure variables. Findings revealed that, 56.9 % of households had limited access to drinking water, 43.3 % lacked improved sanitation, and 48.2 % were without basic hygiene facilities, while only 10.6 % had basic access to all three WASH services. Compared to the richest households, poor households (asset-based index) were 76 % less likely (adjusted odds ratio [AOR]: 0.24, 95 % confidence interval [CI]: 0.16–0.54) to have water access. Households located in the southeastern region had 1.67 (AOR: 1.67, 95 % CI: 1.02–2.74) times higher odds of having water access than those in the southwestern part. Larger households and those headed by fishermen were 39 % (AOR: 0.61, 95 % CI: 0.40–0.93) and 70 % (AOR: 0.30, 95 % CI: 0.14–0.65) less likely to have sanitation access compared with smaller households and those headed by service holders, respectively. Households having adults with primary incomplete or only primary education were significantly associated with reduced access to hygiene and combined WASH facilities, respectively, whereas wealth was a significant factor for WASH facilities. The findings thus underscore the need for targeted interventions including subsidised disadvantaged occupational groups and community-based education programs to improve WASH access in this vulnerable zone.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107792"},"PeriodicalIF":2.5,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of miRNAs dictates the susceptibility/resistance during malaria pathogenesis","authors":"Poonam Kataria ,&nbsp;Amrendra Chaudhary ,&nbsp;Reva Sharan Thakur ,&nbsp;Indu Sharma ,&nbsp;Meenu Kalkal ,&nbsp;Rubika Chauhan ,&nbsp;Waseem Akram Malla ,&nbsp;Kuldeep Singh ,&nbsp;Jyoti Das","doi":"10.1016/j.actatropica.2025.107787","DOIUrl":"10.1016/j.actatropica.2025.107787","url":null,"abstract":"<div><div>Malaria continues to pose a significant global health challenge, with the severity of the disease being influenced by intricate host–parasite interactions. MicroRNAs (miRNAs), which function as post-transcriptional regulators, are pivotal in modulating host immune responses during infection. This study conducted a comparative analysis of miRNA expression profiles in splenocytes of mice infected with two closely related <em>Plasmodium yoelii</em> strains- <em>Py</em>17XL (lethal) and <em>Py</em>17XNL (non-lethal), utilizing small RNA sequencing on day 13 post-infection. A total of 171 and 220 miRNAs were differentially expressed in the <em>Py</em>17XL and <em>Py</em>17XNL groups, respectively, in comparison to uninfected controls. Among these, 79 miRNAs were found commonly dysregulated, while 53 and 86 miRNAs were unique to the lethal and non-lethal <em>P. yoelii</em> infection, respectively. Further, RT-qPCR validation corroborated the expression patterns of selected miRNAs. In <em>Py</em> 17XNL group, miR-875–3p, miR-30a-3p, miR-499–5p, and miR-212–3p were significantly upregulated, whereas miR-6992–5p, miR-704, miR-7676–3p, and miR-1933–3p were predominantly elevated in the <em>Py</em>17XL group. Pathway enrichment analysis indicated that these miRNAs target genes involved in immune-related signalling pathways, including MAPK, mTOR, FoxO, Th-17 cell differentiation and TGF-β signalling. These findings suggest distinct miRNA-mediated regulatory mechanisms underlying lethal and non-lethal malaria outcomes and offer potential molecular targets for therapeutic intervention and biomarker development.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107787"},"PeriodicalIF":2.5,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of Eurytrema coelomaticum found in bovine pancreas in Argentina","authors":"JP Díaz ,&nbsp;LH Olmos ,&nbsp;A Avellaneda-Cáceres ,&nbsp;AJ Pantiu ,&nbsp;EI Figueredo ,&nbsp;JF Micheloud","doi":"10.1016/j.actatropica.2025.107788","DOIUrl":"10.1016/j.actatropica.2025.107788","url":null,"abstract":"<div><div><em>Eurytrema</em> spp. flukes are responsible for economic losses in ruminant production, leading to hyperplasia and hypertrophy of the bile ducts, periductal fibrosis and loss of pancreatic parenchyma. These flukes affect ruminants, small carnivores and occasionally humans. The aim of this study was to perform a precise identification by morphometry and molecular markers in <em>Eurytrema</em> flukes from Argentina, also clarify their phylogenetic relationships. <em>Eurytrema</em> flukes were collected from the pancreatic ducts of breed cattle examined during necropsies in Montecarlo, Misiones province, Argentina. Precise identification of species of the genus <em>Eurytrema</em> is important because some of them can affect humans. Reports of <em>Eurytrema</em> species have in Argentina, Brazil and several Asian countries. In this study, we identified 20 <em>Eurytrema</em> flukes as <em>Eurytrema coelomaticum</em> by morphological characters, collected from Montecarlo. Four individuals were selected for molecular analyses targeted to 18S rRNA and ITS2 genes, with sequence analyses conducted to confirm identification due to the conflicting morphological classification among <em>Eurytrema</em> species. For both genes, we performed multiple alignments with reference sequences, constructed phylogenetic trees and realized an analysis of genetic divergence, based on a pairwise comparison. Phylogenetic analyses based on 18S rRNA sequences revealed that <em>E. coelomaticum</em> from Argentina shows high similarity with sequences from Brazil with low percent of 18S rRNA gene divergence, forming a separate group from <em>E. coelomaticum</em> found in China and Vietnam, no sequence variation was observed among this Asian <em>E. coelomaticum</em> isolates. This indicates there is evolutionary divergence between <em>E. coelomaticum</em> from South America and Asia. This study presents, for the first time, the sequence of the ITS2 gene for <em>E. coelomaticum</em> from South America, which showed 100% similarity with <em>E. coelomaticum</em> isolated from Vietnam. Findings confirmed that <em>E. coelomaticum</em> is circulating in Argentina, as the presence of <em>E. pancreaticum</em> poses a potential public health risk due to its zoonotic potential. Continued monitoring of <em>Eurytrema</em> species in the country is essential through epidemiological and molecular surveillance.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107788"},"PeriodicalIF":2.5,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}