Acta tropica最新文献

{"title":"Multi-technology parasite detection: The intersection of tradition and innovation","authors":"ChenYu Wang ,&nbsp;RuoFeng Xu ,&nbsp;YuXiang Zeng ,&nbsp;RuiNing He ,&nbsp;Bo Zhang ,&nbsp;YongKang Huang ,&nbsp;ShuCheng Ma ,&nbsp;YuXuan Zuo ,&nbsp;YuJun Deng ,&nbsp;YanYu Wang ,&nbsp;Tao Xiong","doi":"10.1016/j.actatropica.2025.107652","DOIUrl":"10.1016/j.actatropica.2025.107652","url":null,"abstract":"<div><div>Parasitic diseases remain a serious global public health problem, especially in the Asian, African, and Latin American regions with relatively poor medical conditions. Timely, accurate, and rapid detection is the key to parasitic disease prevention and control, and the use of novel technologies to study worm resistance is of great significance for the clinical treatment of patients. This study provides an overview of the research progress in parasite detection technologies, encompassing both traditional and emerging methods. In the future, integrating multiple technologies and adopting intelligent detection approaches will be instrumental in advancing parasitic disease detection. Future technologies aim to provide a reference for enhancing the detection of parasitic infections and contribute to the protection of human health and public health safety while offering new ideas and methods for diagnosing parasitic diseases.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107652"},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum nitric oxide in chronic Chagas disease: a scoping review","authors":"Patrícia Carla de Oliveira ,&nbsp;Karen Dyminski Parente Ribeiro ,&nbsp;Lilian Pereira Ferrari ,&nbsp;Fernanda Tomiotto-Pellissier ,&nbsp;Iara Jose de Messias Reason","doi":"10.1016/j.actatropica.2025.107673","DOIUrl":"10.1016/j.actatropica.2025.107673","url":null,"abstract":"<div><div>Nitric oxide (NO) plays a complex role in the pathophysiology of chronic Chagas disease (CD), contributing to both host defense and tissue damage. Thus, understanding its clinical implications may support therapeutic strategies. The objective of this scoping review was to map and synthesize the available evidence on the clinical impact of serum NO levels in individuals with chronic CD. A scoping review was conducted following PRISMA-ScR guidelines, in which a systematic search of PubMed, Scopus, and Web of Science was performed. The inclusion criteria were studies that quantified NO or its synthetizing enzymes (NOS) in individuals with chronic CD irrespective of the publication year or the language. Studies that did not assess the levels of NO, its metabolites or NOS, in addition to <em>in vivo</em> and <em>in vitro</em> experimental studies, were excluded. The findings from this scoping review showed that individuals with chronic CD have high serum NO levels, suggesting that excessive production of this marker may lead to oxidative stress and play a role in the pathophysiological process associated with cardiac and digestive complications. Combined therapies using antiparasitic drugs with antioxidants have shown a greater reduction in NO levels compared to conventional drug therapy, indicating a potential to reduce oxidative damage and consequently slow disease progression. Nonetheless, additional studies at various disease stages are necessary to confirm the effectiveness of this approach.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107673"},"PeriodicalIF":2.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype patterns and evolutionary rates: uncovering Japanese encephalitis virus spread across Asia's climate regions","authors":"Shijili Mambully ,&nbsp;Varsha Ramesh ,&nbsp;Swati Rani ,&nbsp;Mehnaj Khatoon ,&nbsp;Jayashree A ,&nbsp;Archana V Patil ,&nbsp;Azhahianambi Palavesam ,&nbsp;Pinaki Prasad Sengupta ,&nbsp;Sharanagouda S Patil ,&nbsp;Suresh KP","doi":"10.1016/j.actatropica.2025.107676","DOIUrl":"10.1016/j.actatropica.2025.107676","url":null,"abstract":"<div><div>Japanese Encephalitis Virus (JEV) is a highly endemic zoonotic virus, consistently found in Asia and parts of the Western Pacific, and it’s a major cause of human encephalitis. JEV belongs to a family of antigenically related viruses such as West Nile Virus (WNV), Murray Valley encephalitis virus (MVEV), and Aichi Lake Fever Virus (ALFV) and is transmitted by mosquitoes. Persistent outbreaks of the disease necessitate detailed studies to understand their transmission dynamics and develop effective prevention strategies. This study explores the evolutionary dynamics and spatial transmission of JEV, concentrating on the envelope protein (E) structural gene sequences obtained from across Asia’s diverse climatic regions. Evolutionary modeling of the JEV E gene revealed a higher evolutionary rate in tropical regions compared to temperate regions, with nucleotide substitution rates estimated at 1.12 × 10⁻³ per site per year for tropical regions and 5.284 × 10⁻⁴ for temperate regions. The time to the most recent common ancestor (tMRCA) was traced to 1796 from Korea for temperate regions, and 1865 from Indonesia for tropical regions. Among the five genotypes of JEV, Genotype I (GI) and III (GIII) were established all over Southeast Asia; moreover, GI revealed a higher evolutionary rate, reflecting its adaptability to diverse ecological niches. The phylogeographic analysis highlighted significant contributions to virus diffusion by China, Korea, and Japan in temperate zones and by Vietnam in tropical zones. By analyzing genetic sequences from various regions and time periods, this study delivered valuable intuitions into transmission pathways. The findings highlighted the necessity of ongoing surveillance and evolutionary monitoring to track the spread and emergence of novel variations of JEV, which are crucial not just for managing JEV outbreaks but also for guiding immunization programs and public health initiatives.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107676"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-155-chitosan polyplex as a novel therapeutic modality against Leishmania major: A feasibility study","authors":"Yasamin Rahmani ,&nbsp;Samira Mohammadi-Yeganeh ,&nbsp;Farshid Yeganeh ,&nbsp;Azam Pourabbasi Ardekan ,&nbsp;Ameneh Koochaki ,&nbsp;Milad Taghizadeh-Anvar ,&nbsp;Mostafa Haji Molla Hoseini","doi":"10.1016/j.actatropica.2025.107674","DOIUrl":"10.1016/j.actatropica.2025.107674","url":null,"abstract":"<div><h3>Background</h3><div>Leishmaniasis is a neglected tropical disease affecting millions of people worldwide. Macrophages play a crucial role in the life cycle of <em>Leishmania</em> and host-parasite interactions. <em>Leishmania</em> parasites have evolved mechanisms to modulate macrophage miRNA, including miR-155, in order to evade immune responses. Modulation of miR-155 levels could potentially restore balanced immune responses while enhancing the host's ability to clear parasites.</div></div><div><h3>Objective</h3><div>This study aims to evaluate the antileishmanial effects of the miR-155 chitosan polyplex (miR-155 CP) <em>in vitro</em>.</div></div><div><h3>Methods</h3><div>The antileishmanial activity of miR-155 CP synthesized by the coacervation method was assessed against <em>Leishmania major</em> by analyzing the IL-12 and IL-10 secretion, nitric oxide (NO) and urea production, as well as cell death rate on infected RAW 264.7 cells <em>in vitro.</em></div></div><div><h3>Results</h3><div>The polyplexes were produced with high transfection efficiency. MiR-155 was up-regulated over 20-fold in healthy macrophages and 6-fold among infected macrophages. The mean cell death index among treated infected cells (65.5 ± 4.5) increased significantly compared to the infected control group (25±5). Treatment with miR-155 CP triggered the production of IL-12 and NO among uninfected and infected macrophages.</div></div><div><h3>Conclusion</h3><div>Collectively, our findings suggest that induction of miR-155 expression via miR-155 CP may influence cellular mechanisms in two ways: eliminating infected macrophages and enabling uninfected ones to manage the parasite.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107674"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of epizootic hemorrhagic disease and serotypes of epizootic hemorrhagic disease virus in Pakistan","authors":"Muhammad Ishaq ,&nbsp;Syed M. Jamal ,&nbsp;Liana Teodori ,&nbsp;Alessandra Leone ,&nbsp;Barbara Bonfini ,&nbsp;Massimo Spedicato ,&nbsp;Giovanni Savini","doi":"10.1016/j.actatropica.2025.107675","DOIUrl":"10.1016/j.actatropica.2025.107675","url":null,"abstract":"<div><div>This study investigated seroprevalence and serotypes of Epizootic hemorrhagic disease virus (EHDV) in ruminants in North-Western Pakistan. A total of 616 serum samples were tested for the presence of antibodies against EHDV and neutralizing antibodies against 7 EHDV serotypes (EHDV-1, 2, 4, 5, 6, 7 and 8) using competitive ELISA and VNT, respectively. The overall seroprevalence of EHDV was found to be 35.9 %. Seroprevalence of EHDV in cattle, buffalo and goats was found as 66.9 %, 21.3 % and 4.1 %, respectively. In multivariate analysis, the seroprevalence of EHDV was found 12.8 (95 % CI = 6.1–27) times higher in cattle than buffalo and 3.3 (95 % CI = 1.4–10) times higher in buffalo than goats. The seroprevalence was found to be 6 (95 % CI = 3.2–11.3) times higher in old animals than in young ones. The seroprevalence was recorded as 4.8 (95 % CI = 2.3–10) and 2.1 (95 % CI = 1.3–3.6) times higher in the Southern and Central zones, respectively than in the Northern zone of the study area. Neutralizing antibodies against six different serotypes of EHDV (EHDV-1, 2, 5, 6, 7 and 8) were detected. Neutralizing antibodies against EHDV-6 was found to be the most prevalent (62.1 %) and those against EHDV-1 as the least prevalent (11.2 %). This study shows that Epizootic hemorrhagic disease (EHD) is prevalent in Pakistan and at least six different serotypes of EHDV have circulated or are circulating in the country. Virological studies are required to investigate the currently circulating serotypes of EHDV in the country.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107675"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Comparative analysis of population genetics between Schistosoma japonicum-infected and uninfected Oncomelania hupensis snails using microsatellites”","authors":"Meng-Rui Huang ,&nbsp;Yu-Xin Qi ,&nbsp;Qing Xu,&nbsp;Ze-Ting Liu,&nbsp;Xiao-Yan Wu,&nbsp;Han-Xiang Zhang,&nbsp;Da-Bing Lu","doi":"10.1016/j.actatropica.2025.107658","DOIUrl":"10.1016/j.actatropica.2025.107658","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107658"},"PeriodicalIF":2.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neglecting animal sporotrichosis notification in Brazil: A challenge for one health surveillance","authors":"Paulo Ricardo Martins-Filho ,&nbsp;Silvia Maria da Silva Sant'Ana Rodrigues ,&nbsp;Thialla Andrade Carvalho ,&nbsp;Francy Waltilia Cruz Araújo ,&nbsp;André Luiz de Jesus Morais ,&nbsp;Ana Fátima Souza Melo de Andrade ,&nbsp;Danilo Santos de Sousa ,&nbsp;Martha Débora Lira Tenório ,&nbsp;Janiele de Sá Ferreira ,&nbsp;Roseane Nunes de Santana Campos","doi":"10.1016/j.actatropica.2025.107665","DOIUrl":"10.1016/j.actatropica.2025.107665","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107665"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of Clonorchis sinensis infection and subsequent treatment: a qualitative study in Guangxi, China","authors":"Yu-Ying Zhu ,&nbsp;Jia-Guang Zhao ,&nbsp;Lu-Yuan Zhao ,&nbsp;Chang-Hai Zhou ,&nbsp;Jun Meng ,&nbsp;Zhi-Hua Jiang ,&nbsp;Shi-Zhu Li ,&nbsp;Men-Bao Qian","doi":"10.1016/j.actatropica.2025.107664","DOIUrl":"10.1016/j.actatropica.2025.107664","url":null,"abstract":"<div><div>Clonorchiasis is caused by the ingestion of raw freshwater fish containing the metacercariae of <em>Clonorchis sinensis</em>. High burden is exerted by clonorchiasis in East Asia, especially in China. Treatment is compulsory to control the morbidity, while mass drug administration and selective drug administration are recommended in high endemic areas. Few qualitative studies have been implemented to explore the knowledge, attitudes, and practices (KAP) towards clonorchiasis as well as the treatment. In 2024, a qualitative cross-sectional study was conducted in two clonorchiasis-endemic villages of Binyang county, Guangxi, China. A total of ten focus group discussions (FGDs) were carried out, involving 52 participants (10 village leaders, 2 village doctors and 40 villagers). Interview focused on the knowledge on harm, transmission, treatment and prevention of clonorchiasis, and attitudes and practices to ingesting raw freshwater fish (<em>“yusheng”</em>) and treatment. Recorded data were transcribed and analyzed manually by using thematic analysis. About half of the participants (25/52) reported the practice of consuming <em>“yusheng”</em>, with a higher percentage in males (84.00 %, 21/25) than in females (16.00 %, 4/25), which is relevant to the differences in dietary predilection and social roles. Especially, a dinner party at festivals is an important contributor in male’s ingestion of “<em>yusheng</em>”. Although 76.92 % of the participants (40/52) acknowledged the potential health risks associated with the ingestion of <em>“yusheng”</em>, most were unaware of the severe morbidities caused by <em>C. sinensis</em>. Furthermore, some people hold a misconception on the additional nutritional value of “<em>yusheng</em>”. Most of the participants (88.46 %, 46/52) had not undergone treatment for clonorchiasis. The participants tended to take medicine while a definite diagnosis of infection was made, and especially low compliance was demonstrated in the individuals without the practice of ingesting <em>“yusheng”</em>. Additionally, the price of the drug and delivery approach also impacted their choices. Village leaders and doctors believed the importance to implement control for clonorchiasis, and health education was suggested to increase the compliance in treatment. In the studied areas, the endemicity of clonorchiasis is driven by the cultural identification, inadequate knowledge, as well as misconception, which demonstrate significant difference in different populations. The choice of treatment is highly impacted by the definite diagnosis and individual’s dietary habit, which may hinder the adoption of drug administration at population level. To control and prevent clonorchiasis, health education is suggested to increase the knowledge including the harm and importance of subsequent drug administration.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107664"},"PeriodicalIF":2.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-resolution GIS and machine learning approach for targeted disease management and localized risk assessment in an urban setup: A case study from Bhopal, Central India","authors":"Deepanker Das ,&nbsp;Siddhartha Maiti ,&nbsp;Devojit Kumar Sarma","doi":"10.1016/j.actatropica.2025.107662","DOIUrl":"10.1016/j.actatropica.2025.107662","url":null,"abstract":"<div><div>Predicting dengue distribution based on environmental factors is crucial for effective vector control and management as environmental factors like temperature, demographics, and artificial changes such as roads and buildings significantly influence dengue distribution. The use of new, emerging machine-learning techniques can aid in accurately predicting these cases and developing early warning systems. In this study, we divided our study area, Bhopal city, into 643 polygons of one square kilometre area and collected data on environmental and other factors. Dengue cases from 2012 to 2022 were mapped into these units and divided them into five categories. To find the best predictive model, we evaluated popular machine learning algorithms such as support vector machine (SVM), logistic regression, neural networks, random forest, k-Nearest Neighbors (kNN), and tree using parameters like area under the receiver operating characteristic (ROC) curve (AUC), classification accuracy (CA), F1 score, precision, and recall. The neural network performed the best, with an AUC of 0.921, CA of 0.755, F1 score of 0.740, precision of 0.732, and recall value of 0.755 and was thus selected for future predictions. Among the predictors, building area, population and road density had the highest influence, followed by minimum, maximum, and average temperatures in decreasing order of importance. The machine learning approach neural network effectively predicted the historical dengue distribution considering both landscape and climatic variables for an urban settings like Bhopal. This approach holds potential for application in other cities as well, highlighting the increasing importance of machine learning and predictive modelling in public health.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107662"},"PeriodicalIF":2.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dendrimer-based dual delivery system for artesunate and fluconazole: Effective malaria treatment in a murine model of Plasmodium berghei","authors":"Fatemeh Babapour ,&nbsp;Faride Khanabadi ,&nbsp;Shohreh Fahimirad ,&nbsp;Seyedeh Shaghayegh Hosseini ,&nbsp;Taher Elmi","doi":"10.1016/j.actatropica.2025.107663","DOIUrl":"10.1016/j.actatropica.2025.107663","url":null,"abstract":"<div><h3>Purpose</h3><div>The increasing prevalence of <em>Plasmodium</em> resistance to conventional artemisinin-based combination therapies (ACTs) presents a significant threat to global malaria control. To address this, we developed a nanocarrier-based co-delivery system (ND-ARS/FLZ) encapsulating artesunate (ARS) and fluconazole (FLZ), aimed at enhancing therapeutic efficacy and overcoming drug resistance in a murine model of <em>Plasmodium berghei</em> infection. The rationale for combining ARS, an antimalarial, with FLZ, an antifungal, stems from recent studies indicating potential synergistic effects on <em>Plasmodium</em> metabolism and drug resistance mechanisms.</div></div><div><h3>Methods</h3><div>The ND-ARS/FLZ nanocarrier was synthesized using a second-generation dendrimer platform, and its structural characteristics were analyzed using Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and scanning electron microscopy (SEM). Encapsulation efficiency and drug loading were also determined. Antiplasmodial efficacy was assessed by quantifying parasitemia on day 4 post-treatment in infected mice. Histopathological analysis of liver tissue was performed to evaluate safety and biocompatibility. Statistical significance was determined using SPSS.</div></div><div><h3>Results</h3><div>The ND-ARS/FLZ nanocarrier exhibited a spherical morphology with a mean diameter of 137 nm and demonstrated sustained drug release, achieving 98 % FLZ and 92 % ARS release within 42 h. At a dose of 400 mg/kg (80 mg ARS + 44 mg FLZ), ND-ARS/FLZ achieved complete parasitemia clearance (100 %), significantly outperforming monotherapies (<em>P</em> &lt; 0.01). The estimated ED₅₀ was 146 mg/kg, indicating potent antimalarial activity. No histopathological signs of hepatotoxicity were observed (<em>P</em> &gt; 0.05), supporting the formulation’s favorable safety profile. The PEGylated dendrimer core, combined with a citric acid-functionalized surface, enhanced the antimalarial efficacy of encapsulated drugs while concurrently minimizing off-target toxicity.</div></div><div><h3>Conclusion</h3><div>The ND-ARS/FLZ nanocarrier exhibits potent antimalarial activity and a promising safety profile, positioning it as a potential candidate for treating drug-resistant malaria. The synergistic drug combination, coupled with nanocarrier-mediated delivery, offers a novel approach to overcoming resistance. Future studies should further explore the pharmacokinetics, host-parasite interactions, and mechanisms of action using techniques such as mass spectrometry, NMR, and in vivo imaging to facilitate preclinical advancement.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107663"},"PeriodicalIF":2.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}