Acta tropica最新文献

{"title":"First detection of Nyssorhynchus rondoniensis (Diptera: Culicidae) in southern Venezuela and its infection with Plasmodium falciparum","authors":"Jan E. Conn ,&nbsp;Sara A. Bickersmith ,&nbsp;Jorge E. Moreno ,&nbsp;Maria Anice Mureb Sallum ,&nbsp;Victor Sanchez ,&nbsp;Nelson Moncada ,&nbsp;María Eugenia Grillet","doi":"10.1016/j.actatropica.2025.107802","DOIUrl":"10.1016/j.actatropica.2025.107802","url":null,"abstract":"<div><div>Ongoing efforts to control vectors and eliminate malaria in the Neotropics are hampered by challenges that include accurate mosquito identification and acquisition of relevant local ecological data. In Bolivar state, southern Venezuela, 88 specimens of a potential malaria vector, <em>Nyssorhynchus rondoniensis,</em> were detected in several villages, identified by <em>COI</em> barcode sequences, and one mosquito was found infected with <em>Plasmodium falciparum</em> by real-time PCR, for an infection rate of 1.14 %. Increasingly, deforestation for a range of anthropogenic activities, especially mining, urbanization, and agriculture, leads to change in anopheline diversity. The main reasons for concern in the detection of an infected specimen of a new potential vector species in the highly modified gold-mining environment of southern Venezuela are its detection in different years, in both rainy and dry seasons, in peridomestic and forest fringe habitat, its anthropophilic behavior, and early and late evening biting times. This mosquito species was recently described for the first time and is only known from Acre and Rondônia states, western Brazil, until now. The most likely explanation for the large geographical gap between southern Venezuela and western Brazil is that <em>Ny. rondoniensis</em> is present but has been misidentified as another morphologically similar species.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107802"},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amazonian sandflies (Diptera: Psychodidae) in a forest extraction area, and the first detection of Leishmania DNA in Pintomyia serrana","authors":"Eduardo Alcici de Souza ,&nbsp;Ana Carolina Gomes Carneiro ,&nbsp;Edmilson Pereira Barroso ,&nbsp;Márcia Moreira de Ávila ,&nbsp;Jailson Ferreira de Souza ,&nbsp;Janaína Estevo de Oliveira ,&nbsp;Leonardo Augusto Kohara Melchior ,&nbsp;Paloma Helena Fernandes Shimabukuro ,&nbsp;Eunice Aparecida Bianchi Galati ,&nbsp;Bruno Leite Rodrigues ,&nbsp;Andreia Fernandes Brilhante","doi":"10.1016/j.actatropica.2025.107806","DOIUrl":"10.1016/j.actatropica.2025.107806","url":null,"abstract":"<div><h3>Introduction</h3><div>In Brazil, the sandfly species' greatest diversity and richness is concentrated in the Amazon region. This is also the greatest diversity of causative agents of American Cutaneous Leishmaniasis (ACL). The present study aimed to identify aspects of the sandfly fauna and the detection of <em>Leishmania</em> in the forest extraction area in the municipality of Xapuri, Acre, Western Amazon, complementing previous studies that were undertaken in the same region.</div></div><div><h3>Methods</h3><div>To collect the insects, four methods were used. These were Center for Disease Control and Prevention (CDC) type light traps, white Shannon traps, manual aspiration in tree trunks, and sticky traps. For the investigation of infection with <em>Leishmania</em>, DNA samples from females were submitted to polymerase chain reactions (PCR), with amplification of the Internal Transcribed Spacer 1 (ITS1) and <em>cytochrome b (cytb</em>) regions to detect <em>Leishmania</em> DNA and blood meal sources, respectively.</div></div><div><h3>Results</h3><div>A total of 4377 insects that were collected belonged to 15 genera and 46 species, with <em>Nyssomyia shawi, Trichophoromyia</em> sp. and <em>Psychodopygus carrerai</em> the most abundant. The highest population density of specimens collected was in CDC light traps (<em>n</em> = 2459), and the lowest was in sticky traps (<em>n</em> = 16). As for the molecular analysis, <em>Ps. davisi</em> was positive for a species of Trypanosomatidae, while <em>Pintomyia serrana</em> showed <em>Leishmania</em> (<em>Viannia</em>) <em>guyanensis</em> DNA. For blood meal sources, we detected the DNA of <em>Tamandua tetradactyla</em> in <em>Psathyromyia dendrophyla</em>, and <em>Homo sapiens</em> in <em>Lutzomyia sherlocki, Ps. carrerai</em> and <em>Ps. davisi.</em></div></div><div><h3>Conclusion</h3><div>The diversity of the sandfly fauna in Xapuri remains high, with new records of phlebotomine species and infections in different species of sandflies, so the region, as it is a hotspot for cutaneous leishmaniasis in Acre, must be constantly monitored.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107806"},"PeriodicalIF":2.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-emulsifying and lyotropic nanocarriers enhance solubility and antiparasitic efficacy of artesunic acid against different strains of Schistosoma mansoni","authors":"Sheila de Andrade Penteado Corrêa ,&nbsp;Tiago Manuel Fernandes Mendes ,&nbsp;Rosimeire Nunes de Oliveira ,&nbsp;Marilia Bergamini Valentini ,&nbsp;Thaís F.R. Alves ,&nbsp;Juliana Ferreira de Souza ,&nbsp;Marco Vinícius Chaud ,&nbsp;Silmara Marques Allegretti","doi":"10.1016/j.actatropica.2025.107808","DOIUrl":"10.1016/j.actatropica.2025.107808","url":null,"abstract":"<div><div>Schistosomiasis currently relies on the long-term use of a single drug, praziquantel (PZQ). Artesunic acid (AcART) has emerged as a promising alternative, as it is efficient against juvenile worms. However, like PZQ, it has low aqueous solubility. Self-Emulsifying Drug Delivery Systems (SEDDS) and Lyotropic Liquid Crystal Nanoparticles (NPs-LLC) present advantages for oral administration, including enhanced solubility and protection of the drug from first-pass metabolism. This study compared the <em>in vitro</em> and <em>in vivo</em> efficacy of AcART incorporated into SEDDS and NPs-LLC against juvenile and adult worms of two different <em>Schistosoma mansoni</em> strains. <em>In vitro</em> phenotypic assays with AcART incorporated into both SEDDS and NPs-LLC demonstrated a progressive decrease in parasite viability, significant tegumental changes, inhibition of oviposition, and 100 % mortality at a concentration 6.25 µg/mL. <em>In vivo</em> treatments performed at 21- and 60-days post-infection with AcART formulated in SEDDS and NPs-LLC led to a significant reduction in worms’ burden and eggs eliminated in feces compared to the control group. The SEDDS nanosystems (drug-free) influenced the <em>in vitro</em> results, whereas the NPs-LLC (drug- free) did not influence any of the assays. Our findings demonstrate that these nanosystems enhance the efficacy of AcART against both BH and SE strains of <em>S. mansoni</em>, even at substantially lower concentrations than conventional formulations. This represents an innovative approach for the treatment of schistosomiasis, with potential applications to other neglected diseases.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107808"},"PeriodicalIF":2.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological assessment of the first year of mandatory notification of chronic Chagas disease in Alagoas, Northeast Brazil","authors":"Julyane Corato da Silva ,&nbsp;Marina Taissa Santiago da Silva ,&nbsp;Laryssa Bezerra dos Santos ,&nbsp;Maria Gabriela Gomes Abdala ,&nbsp;Anthony Batista de Oliveira Lopes ,&nbsp;Gilsan Aparecida de Oliveira ,&nbsp;Francisca Hildemagna Guedes-da-Silva ,&nbsp;Thaís da Silva Rigoni ,&nbsp;Flávia S. Damasceno ,&nbsp;João Araújo Barros-Neto ,&nbsp;Arnaldo Cézar Couto ,&nbsp;Andrey Ferreira da Silva ,&nbsp;Müller Ribeiro-Andrade ,&nbsp;Maria Cláudia Silva","doi":"10.1016/j.actatropica.2025.107805","DOIUrl":"10.1016/j.actatropica.2025.107805","url":null,"abstract":"<div><div>Chagas disease (CD), caused by the protozoan parasite <em>Trypanosoma cruzi</em>, affects &gt;7 million individuals worldwide. The disease progresses from an acute phase to a chronic phase, which may manifest as indeterminate, cardiac, digestive or cardiodigestive forms. Although CD is endemic in Brazil, only acute cases were subject to mandatory notification. In 2020, Brazil expanded mandatory notifications to include chronic cases; however, implementation in the State of Alagoas (AL), Northeast of Brazil, began only in January 2023. Here, we performed an epidemiological assessment of the first year (January 2023 to January 2024) of the mandatory notification of chronic CD in AL. Our data show that most of the 104 reported cases occurred among Afro-Brazilian women aged 40–59 with no or low levels of formal education. Despite the chronic nature of the disease, most diagnoses were recent and based on serological methods, primarily ELISA in combination with immunofluorescence assays. The cardiac form was the most frequently documented clinical manifestation, and 12 individuals succumbed to cardiac complications associated with the disease. These findings highlight the significant prevalence of chronic CD among adults in the State of Alagoas (AL) and underscores its role in elevating mortality risk due to cardiac and cardiodigestive complications in this population.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107805"},"PeriodicalIF":2.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital PCR-based detection of Cryptosporidium in Pancreatic Tissue and Saliva Samples of Cancer patients; Pancreatic cryptosporidiosis","authors":"Tufan Gumus ,&nbsp;Deniz Ece ,&nbsp;Can Muftuoglu ,&nbsp;Ufuk Mert ,&nbsp;Ecem Kalemoglu ,&nbsp;Goksever Akpinar ,&nbsp;Milad Asadi ,&nbsp;Tolga Coskun ,&nbsp;Hamid Alizadeh ,&nbsp;Alper Uguz ,&nbsp;Ayse Caner","doi":"10.1016/j.actatropica.2025.107804","DOIUrl":"10.1016/j.actatropica.2025.107804","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Cryptosporidium</em> spp. is a protozoan parasite known to cause gastrointestinal infections, particularly in immunocompromised individuals such as cancer patients. It typically infects the small intestine but has been reported in extraintestinal sites, including the biliary tract, and lungs. While <em>Cryptosporidium</em> has been implicated in the pathogenesis of several gastrointestinal cancers, there is very limited data its association with pancreatic ductal adenocarcinoma (PDAC). This study aimed to investigate the presence of <em>Cryptosporidium</em> in pancreatic tissue and saliva samples from PDAC patients using digital PCR (dPCR), a highly sensitive diagnostic tool, and to compare findings with those in distal cholangiocarcinoma (DCC) patients and healthy controls.</div></div><div><h3>Methods</h3><div>A total of 50 participants were enrolled, including 20 PDAC patients, 10 DCC patients, and 20 healthy controls. Pancreatic tissue and saliva samples were collected from PDAC and DCC patients, while only saliva was collected from healthy controls. DNA was extracted from all samples, and the presence of <em>Cryptosporidium</em> was investigated using both quantitative PCR (qPCR) and dPCR. Cancer patients were also analyzed for clinical findings and patients positive for <em>Cryptosporidium</em> spp. in saliva and tissue samples were evaluated clinically.</div></div><div><h3>Results</h3><div><em>Cryptosporidium</em> DNA was detected by qPCR in the saliva of one DCC patient. In contrast, dPCR revealed <em>Cryptosporidium</em> spp. in the saliva of one PDAC and two DCC patients (one overlapping with qPCR), and in the pancreatic tissue of two PDAC patients (whose saliva was negative). No <em>Cryptosporidium</em> spp. was detected in the healthy control group. Although the differences between groups were not statistically significant, the presence of <em>Cryptosporidium</em> in pancreatic tissue was demonstrated for the first time using dPCR. Some positive patients showed respiratory symptoms or were asymptomatic, raising the possibility of subclinical or extraintestinal infection.</div></div><div><h3>Conclusion</h3><div>This study provides the first dPCR-based evidence of <em>Cryptosporidium</em> in pancreatic tissue, suggesting its potential for extraintestinal dissemination in cancer patients. The findings highlight the superiority of dPCR over qPCR for detecting low-abundance pathogens in clinical samples. Although no direct causal link with PDAC was established, the detection of <em>Cryptosporidium</em> in pancreatic tissues warrants further investigation into its potential role in pancreatic carcinogenesis.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107804"},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel invasive virulence of chymotrypsin-like elastase family member 2A from Trichinella spiralis","authors":"Xin Guo,&nbsp;Xin Zhuo Zhang,&nbsp;Yao Zhang,&nbsp;Ru Zhang,&nbsp;Shao Rong Long,&nbsp;Ruo Dan Liu,&nbsp;Jing Cui,&nbsp;Zhong Quan Wang","doi":"10.1016/j.actatropica.2025.107803","DOIUrl":"10.1016/j.actatropica.2025.107803","url":null,"abstract":"<div><div>A novel chymotrypsin-like elastase family member 2A (TsCELA2A) was identified in <em>Trichinella spiralis</em> intestinal infectious larvae (IIL) excretion/secretion protein (ESP), but its properties and function are unclear. The objective of this study was to identify TsCELA2A’s biological characteristics and functions at the process of <em>T. spiralis</em> invasion of enteral epithelium. The results revealed that TsCELA2A has a Tryp_SPc conserved functional domain at 30-273aa, and contains three active sites and three substrate binding sites. rTsCELA2A was expressed and purified. On Western blotting, rTsCELA2A was recognized by anti-rTsCELA2A serum and <em>Trichinella</em>-infected murine serum, and native TsCELA2A in crude proteins of diverse stage worms was recognized by anti-TsCELA2A serum. The results of RT-PCR, Western blotting and immunofluorescence test (IFT) showed that TsCELA2A was expressed at diverse worm stages, and principally located in external cuticle, stichosome and intrauterine embryos of the nematode. rTsCELA2A had enzymatic activity of natural serine protease, which could hydrolyze the substrate N-benzoyl-L-arginine-ethylester (BAEE). rTsCELA2A was specifically combined with enteral epithelium cells, and binding location was primarily located in cellular membrane and cytoplasm. rTsCELA2A mediated larva intrusion of gut epithelium; whereas anti-TsCELA2A antibodies obviously impeded the invasion. Silencing of the TsCELA2A gene significantly decreased the expression and activity of TsCELA2A, and the larval invasion and intestinal adult burdens were reduced respectively by 28.71 and 35.78%. The results indicated that TsCELA2A was an invasive virulence factor and participated in <em>T. spiralis</em> larval intrusion of enteral mucosa, and it might be a candidate vaccine molecule against <em>T. spiralis</em> infection</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107803"},"PeriodicalIF":2.5,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and gametocyte carriage in Plasmodium falciparum cases treated with artemether-lumefantrine alone versus with single-dose primaquine in Ethiopia: A randomized controlled study","authors":"Tinsae Kumsa ,&nbsp;Betelihem Jima ,&nbsp;Girum Tamiru ,&nbsp;Ribka Getu ,&nbsp;Mesay Melaku ,&nbsp;Yilikal Tesfaye ,&nbsp;Bernt Lindtjørn ,&nbsp;Fekadu Massebo","doi":"10.1016/j.actatropica.2025.107800","DOIUrl":"10.1016/j.actatropica.2025.107800","url":null,"abstract":"<div><div>This study evaluated the efficacy of adding primaquine (PQ) to artemether-lumefantrine (AL) for treating uncomplicated <em>Plasmodium falciparum</em> malaria in Ethiopia. Asexual parasite clearance was monitored with microscopy and molecular techniques, while gametocyte clearance was observed solely through microscopy. Genotyping to distinguish recrudescence from reinfection was performed using nested polymerase chain reaction (PCR). A parallel, two-arm, randomized controlled trial with a 1:1 allocation ratio was conducted involving 146 symptomatic patients, with 106 included in the final analysis. The study compared the outcomes of AL plus PQ with those of AL alone over 28 days. PQ treatment was initiated simultaneously with AL therapy. Both treatment groups showed no asexual parasites by day 2 when assessed by microscopy. However, PCR testing detected parasite DNA in 5.8 % (3/52) of the patients in the AL plus PQ group, compared to 18.5 % (10/54) in the AL alone group by day 7 (<em>P</em> = 0.09). PCR-uncorrected cure rate was 100 % for the AL alone and 98.1 % for the AL plus PQ groups (<em>P</em> = 0.31). Gametocyte clearance was faster in the AL plus PQ group, with 100 % clearance by day 7, compared to day 14 in the AL alone group. Notably, only 9.4 % (10/106) of cases had gametocytes at baseline, while this percentage increased to 11.3 % (12/106) by day 1 of post-treatment. The recrudescence rate was 2.8 % (3/106). Although AL alone was as effective as AL plus PQ in eliminating asexual parasites, adding PQ may accelerate gametocyte clearance. However, the low prevalence of gametocyte carriers among symptomatic cases at the study site, combined with the restriction of PQ administration to only those seeking treatment, may limit its overall impact on malaria transmission.</div><div>Trial registration number: PACTR202502642820967</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107800"},"PeriodicalIF":2.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New species, country records, DNA barcoding and host blood meal analysis of some Indian species of Culicoides Latreille subgenus Trithecoides Wirth and Hubert (Diptera: Ceratopogonidae)","authors":"Nabanita Banerjee ,&nbsp;Shuddhasattwa Maitra Mazumdar ,&nbsp;Glenn Bellis ,&nbsp;Abhijit Mazumdar","doi":"10.1016/j.actatropica.2025.107798","DOIUrl":"10.1016/j.actatropica.2025.107798","url":null,"abstract":"<div><div>Light trap collections from four states in India revealed the presence of four species belonging to <em>Culicoides</em> (<em>Trithecoides</em>). Included were a new species from the <em>macfiei</em> group, <em>Culicoides tenebrus</em> sp. nov., which is diagnosed with a dark mid-knee and a yellow scutum featuring a dark anterior quarter, two new country records for India: <em>Culicoides paraflavescens</em> Wirth and Hubert, 1959, and <em>Culicoides laoensis</em> Howarth, 1985, and some variants of the widely distributed species <em>Culicoides palpifer</em> Das Gupta and Ghosh, 1956. Collections contained blood-fed specimens of each of these species, and host blood meal analysis using 16S rRNA revealed that all specimens had fed on cattle. DNA barcodes obtained from some specimens of these species were analysed along with other species from <em>Culicoides</em> (<em>Trithecoides</em>), and a phylogenetic tree based on these sequences supported the status of each of these species. Considerable variation was observed within <em>C. palpifer</em> both in morphology and DNA barcodes, suggesting that this species requires some taxonomic revision. The apparent preference of these four species for cattle hosts suggests that studies into their vector potential are warranted; however, the status of the species of <em>Trithecoides</em> requires clarification before any vector potential studies.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107798"},"PeriodicalIF":2.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhodnius montenegrensis Rosa et al., 2012 (Hemiptera, Triatominae) in Peru: a warning for the transmission of Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) in periurban areas","authors":"Mariane A.L. Ribeiro ,&nbsp;Yago V. dos Reis ,&nbsp;André L.R. Menezes ,&nbsp;Vinicius F. de Paiva ,&nbsp;Jociel Klleyton S. Santana ,&nbsp;João Paulo S. Oliveira-Correia ,&nbsp;Carlos Emilio M. Benavides ,&nbsp;Jader de Oliveira ,&nbsp;Marta Maria G. Teixeira ,&nbsp;Kaio Cesar C. Alevi ,&nbsp;Cleber Galvão ,&nbsp;Dionatas U.de O. Meneguetti ,&nbsp;João Aristeu da Rosa","doi":"10.1016/j.actatropica.2025.107799","DOIUrl":"10.1016/j.actatropica.2025.107799","url":null,"abstract":"<div><div><em>Rhodnius montenegrensis</em> is a sylvatic triatomine and a vector of <em>Trypanosoma cruzi</em>, with records of its invasion into human dwellings. This study reports the first record of <em>R. montenegrensis</em> in Peru. Triatomines were collected from <em>Attalea phalerata</em> palm trees in peri-urban areas of the city of Iñapari, Peru. Seven specimens of <em>R. montenegrensis</em> were collected, and they were found to be infected with <em>T. cruzi</em> and/or <em>T. rangeli</em>. Additionally, some specimens showed human DNA in their gut contents. With this report, the number of triatomine species in Peru increased from 18 to 19, and the number of countries with the presence of <em>R. montenegrensis</em> rose from two to three (Brazil, Bolivia and Peru). The notification of <em>R. montenegrensis</em>, as well as the information related to the infection rate by <em>T. cruzi</em> and the presence of human blood as a food source for this insect vector, highlight the need for attention from the country's vector control programs to this new species recorded in Peru.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107799"},"PeriodicalIF":2.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Against the flow: flooding and its impact on phlebotomine bionomics in the Chaco region, Argentina","authors":"Gomez Gallipoliti Sofia Florencia ,&nbsp;Szelag Enrique Alejandro ,&nbsp;Faraone Janinna ,&nbsp;Ellena Miriam ,&nbsp;Parras Matías ,&nbsp;Salomón Oscar Daniel ,&nbsp;Quintana María Gabriela ,&nbsp;Rosa Juan","doi":"10.1016/j.actatropica.2025.107797","DOIUrl":"10.1016/j.actatropica.2025.107797","url":null,"abstract":"<div><div>Tegumentary leishmaniasis in Argentina presents multiple hotspots and sporadic outbreaks, especially in the Humid Chaco. This study examined ecological and behavioral adaptations of phlebotomine sandflies in flood-affected areas, focusing on breeding sites, vertical stratification, and seasonal abundance, with emphasis on the dominant vector <em>Nyssomyia neivai</em>. The influence of flooding, climate variables, and environmental change on disease risk and control strategies was assessed. Sampling was conducted (2011–2014) in Puerto Antequeras, Chaco, across domicile, peridomicile, and extradomicile ecotopes. From September 2011 to December 2012, eight mini-CDC light traps were installed monthly at ground level (1.5 m) and in canopies (10 m), also soil samples were collected. From January 2013 to December 2014, ground-level mini-CDC and emergence traps were used. Meteorological data informed negative binomial models relating sandfly abundance to climatic and ecological variables. A total of 28462 phlebotomines were captured in the first period and 32009 in the second, with <em>Ny. neivai</em> dominant, particularly in the extradomicile. Abundance was greater at ground level, but canopy fauna was more diverse. Peaks occurred in summer and spring, and abundance increased post-flooding. Minimum temperature positively affected abundance, whereas river height and precipitation were negative predictors. In the first period, soil samples produced only one <em>Ny. neivai</em> male; emergence traps produced 15 individuals emerged from five traps, mainly in the extradomicile, peaking in autumn. Emergence increases with minimum temperature and decreases with precipitation. Flooding may shift breeding sites, and climate change could increase urban transmission risk. Control should integrate vector surveillance with habitat management.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"270 ","pages":"Article 107797"},"PeriodicalIF":2.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}