Acta tropica最新文献

{"title":"Epidemiology of bluetongue virus infection among goat populations in central and western Thailand: Prevalence, risk factors, and serotype analysis","authors":"Thanyaporn Homat ,&nbsp;Yuki Fujisawa ,&nbsp;Chowalit Nakthong ,&nbsp;Phirom Prompiram ,&nbsp;Rassmeepen Phonaknguen ,&nbsp;Tapanut Songkasupa ,&nbsp;Pratana Satitvipawee ,&nbsp;Kridsada Chaichoun ,&nbsp;Kripitch Sutummaporn ,&nbsp;Sudsaijai Kornmatitsuk ,&nbsp;Bunlue Kornmatitsuk","doi":"10.1016/j.actatropica.2025.107558","DOIUrl":"10.1016/j.actatropica.2025.107558","url":null,"abstract":"<div><div>The current status of bluetongue (BT) in Thailand remains unclear, and the serotypes of BT virus (BTV) have not been determined. This study aimed to 1) investigate the epidemiology of BTV infection among goat populations in central and western Thailand; 2) evaluate the test performance of conventional RT-PCR for BTV detection; and 3) identify the BTV serotypes presently circulating in the study area. The herd seroprevalence was 94.6 % (53/56, 95 % CI = 85.3–98.1), while the individual seroprevalence was 74.4 % (434/583, 95 % CI = 70.5–77.6). The odds of BTV infection were higher in goats in the western region (aOR = 10.16, 95 % CI = 5.02–20.55), with the presence of nearby ruminant farms (aOR = 2.21, 95 % CI = 1.23–4.00), and aged ≥3 years (aOR = 14.02, 95 % CI = 6.32–31.08) but lower in goats with an intensive rearing system (aOR = 0.18, 95 % CI = 0.09–0.39). For BTV detection, conventional RT-PCR presented 84.5 % sensitivity and 97.4 % specificity with an AUC of 0.910 (95 % CI = 0.852–0.967). Seven BTV serotypes (2, 3, 4, 7, 12, 16, and 21) were identified. Among these, BTV-2, 12, and 21 were the most prevalent, and BTV-4 was first detected in Southeast Asia. These results illustrated that BT is endemic and spreading widely among goat populations in the study area. Conventional RT-PCR developed in this study proved to be a reliable method for BTV detection. This research provides the first report of BTV serotype distribution in Thailand.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107558"},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melioidosis in people living with diabetes; clinical presentation, clinical course and implications for patient management","authors":"Laura Prideaux ,&nbsp;Megan Sandeman ,&nbsp;Hayley Stratton ,&nbsp;Anthony D Kelleher ,&nbsp;Simon Smith ,&nbsp;Josh Hanson","doi":"10.1016/j.actatropica.2025.107559","DOIUrl":"10.1016/j.actatropica.2025.107559","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Despite the well-established link between diabetes mellitus and melioidosis, the precise impact of diabetes, its complications, and its therapy on the presentation and clinical course of melioidosis is incompletely defined. The influence of glycaemic control on the diverse clinical manifestations and the clinical course of melioidosis in patients with diabetes has also not been examined in detail.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;We examined all cases of culture-confirmed melioidosis in Far North Queensland, Australia between October 1, 2016, and April 30, 2024. We hoped to define the impact of diabetes, its control and its therapy on the patients’ presentation and their clinical course.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;There were 321 cases of culture-confirmed melioidosis during the study period; the patients’ median (interquartile range (IQR)) age was 57 (46–69) years, 212/321 (66 %) were male, 130/321 (41 %) identified as First Nations Australians. Diabetes was the most common risk factor for melioidosis in the cohort (163/321, 51 %); in 19/163 (12 %) this was a new diagnosis. The median (IQR)) glycosylated haemoglobin prior to presentation was 9.1 % (7.2–11.5) and 96/162 (59 %) with complete data had established macrovascular or microvascular complications. People with diabetes were more likely – than people without diabetes – to have involvement of the liver (odds ratio (OR) 95 % confidence interval (CI): 9.68 (2.21–42.46), &lt;em&gt;p&lt;/em&gt; = 0.003), the spleen (OR (95 % CI): 7.32 (1.64–32.80), &lt;em&gt;p&lt;/em&gt; = 0.009) or to have disseminated disease (OR (95 % CI): 2.93 (1.26–6.78), &lt;em&gt;p&lt;/em&gt; = 0.01). However, people with diabetes were no more likely than people without diabetes to require intensive care unit admission (OR (95 % CI): 0.82 (0.47–1.42), &lt;em&gt;p&lt;/em&gt; = 0.48) or to die before hospital discharge (12/163 (7 %) versus 19/158 (12 %), OR (95 % CI): 0.58 (0.27–1.24), &lt;em&gt;p&lt;/em&gt; = 0.16). Only 58/163 (36 %) with diabetes had specialist endocrinology review during their hospitalisation and only 22/72 (31 %) with accessible data had good glycaemic control (glycosylated haemoglobin ≤7 %) in the 12 months after discharge, increasing their risk of subsequent diabetic complications. Of the 151 people with diabetes surviving their hospitalisation, 26 (17 %) died, at a median (IQR) of 1.0 (0.40–4.1) years after discharge. Of the individuals with diabetes who had completed five years of follow up, 21/60 (35 %) had died at a median (IQR) age of 67 (51–84) years.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;Individuals with diabetes and melioidosis are more likely to have liver and spleen abscesses and disseminated disease than individuals without diabetes, manifestations that appear to be linked directly to glycaemic control. In Australia's well-resourced health system &lt;10 % of patients with diabetes and melioidosis will die from their infection. However, five-year all-cause mortality in individuals with diabetes who survi","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107559"},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Wolbachia on mitochondrial DNA variation in Aedes albopictus (Diptera: Culicidae)","authors":"Zu Ma ,&nbsp;Jian Gao ,&nbsp;Ge Wang ,&nbsp;Minghui Zhao ,&nbsp;Dan Xing ,&nbsp;Tongyan Zhao ,&nbsp;Hengduan Zhang","doi":"10.1016/j.actatropica.2025.107561","DOIUrl":"10.1016/j.actatropica.2025.107561","url":null,"abstract":"<div><div><em>Wolbachia</em> species are symbiotic bacteria that are commonly found in arthropods and nematodes and live inside their cells. In nature, endosymbiont–host interactions and dynamics are complex, often depending on environmental conditions and evolutionary history. Both <em>Wolbachia</em> and mitochondrial DNA are maternally inherited in cells, and after a long period of coexistence, the presence of <em>Wolbachia</em> may have an impact on mitochondrial sequence diversity, thereby confounding mtDNA-based host phylogeny. The universal and typing primers for the <em>wsp</em> gene were used for PCR amplification, the number of positive samples was counted, and the infection pattern was analysed. The mitochondrial DNA diversity of four groups (<em>Wolbachia</em>-infected and uninfected samples, as well as between singly and double infected samples.) was analysed. PACo and ParaFitGlobal tests were used to explore evolutionary associations. The overall prevalence of <em>Wolbachia</em> in the 22 natural populations was 94.2 %, with Type A, Type B and <em>A</em> × <em>B</em> mixed infections detected in <em>Aedes albopictus</em> and coinfection between <em>w</em>AlbA and <em>w</em>AlbB prevalent. The mitochondrial DNA haplotype associated with <em>Wolbachia</em> (Hap1) became the dominant haplotype and was the most abundant and widely distributed in the population. The linkage map showed the predominant haplotype, Hap1, was more closely associated with <em>w</em>AlbA than with <em>w</em>AlbB. Neutral evolution deviated significantly from zero. The diversity of mtDNA COI genes associated with <em>Wolbachia</em> infection was reduced. <em>Wolbachia</em> infection may lead to the selective sweep of mitochondrial DNA in <em>Ae. albopictus</em>.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107561"},"PeriodicalIF":2.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the prevalence of Echinococcus multilocularis in free-roaming dogs in the human alveolar echinococcosis endemic region of Turkey","authors":"Muzaffer Akyuz,&nbsp;Hamza Avcioglu","doi":"10.1016/j.actatropica.2025.107560","DOIUrl":"10.1016/j.actatropica.2025.107560","url":null,"abstract":"<div><div>As free-roaming dogs may play a pivotal role in the transmission of alveolar echinococcosis (AE) to humans, the detection of the parasite in dogs is important for the control of the disease. The objective of this study was to ascertain the current situation of <em>E. multilocularis</em> in free-roaming dogs in the northeastern region of Türkiye which is endemic for human AE.</div><div>The study area comprises the provinces of Ağrı, Ardahan, Bayburt, Erzincan, Erzurum, Iğdır and Kars. Between 2019 and 2020, 1069 individual fecal samples collected from free-roaming dogs were analyzed by sequential flotation/sieving method. Each taeniid egg-positive sample was then subjected to PCR for the amplification of partial fragments of 12S rRNA and NAD1 of <em>E. multilocularis</em>. The regional prevalence of <em>E. multilocularis</em> was 8.7 % (93/1069), while the rates of provinces were as follows: Bayburt [14.4 % (15/104)], Iğdır [13.2 % (14/106)], Kars [10.4 % (12/115)], Erzurum [8.3 % (35/420)], Erzincan [6.9 % (7/101)], Ağrı [6.9 % (8/116)] and Ardahan [1.9 % (2/107)].</div><div>This study demonstrates the high burden of <em>E. multilocularis</em> in free-roaming dogs and highlights the potential crucial role of dogs in the transmission of AE, which is a significant public health concern for humans in the region. These findings emphasize the necessity to design control strategies for AE in the region that cover both rural and urban areas and focus specifically on free-roaming dogs.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"264 ","pages":"Article 107560"},"PeriodicalIF":2.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disease burden of brucellosis among the elderly in Inner Mongolia, China: A cross-sectional data study from 2016 to 2020","authors":"Zhenjie Wu ,&nbsp;Yifan Zhao ,&nbsp;Yanling Li,&nbsp;Xinyan Wang,&nbsp;Xuemei Wang","doi":"10.1016/j.actatropica.2025.107556","DOIUrl":"10.1016/j.actatropica.2025.107556","url":null,"abstract":"<div><div>Brucellosis is a zoonotic infection with an annually increasing incidence in the elderly. However, few research teams studied the brucellosis disease burden on this elderly population. This study assesses the brucellosis disease burden of elderly patients in Inner Mongolia, to explore its influencing factors, and provide a scientific basis for effectively reducing the disease burden in this demographic. This study described the disease burden of brucellosis in the elderly population in Inner Mongolia from 2016 to 2020. The incidence of brucellosis among the elderly showed an annually increasing trend, with concentrated incidences from April to July each year. The male and 60–69 age groups exhibit a high prevalence of brucellosis, while Xilingol, Ulanqab, and Hohhot exhibited high disease burdens. Meanwhile, Bayannur exhibited the fastest disease burden growth. Precipitation and the value of total pastoral production were positively correlated with the disease burden. In contrast, the sunshine duration, the number of health institutions, and GDP per capita exhibited negative correlations with the disease burden. The growing brucellosis incidence and disease burden among the elderly in Inner Mongolia is a serious issue. Relevant departments and agencies should focus on protecting the male population aged 60 to 69. Continuous attention should be directed to the western regions to prevent the emergence of new high-risk areas. Strengthening the rural healthcare system through increased medical personnel and financial investment is crucial to lowering the risk of brucellosis and alleviating its impact on the elderly population.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107556"},"PeriodicalIF":2.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasma gondii in urban and rural bats from São Paulo state, Brazil","authors":"Danilo Alves de França,&nbsp;Benedito Donizete Menozzi,&nbsp;Helio Langoni","doi":"10.1016/j.actatropica.2025.107557","DOIUrl":"10.1016/j.actatropica.2025.107557","url":null,"abstract":"<div><div>The aim of this study was to detect <em>Toxoplasma gondii</em> in organs of urban and rural bats from 17 cities in São Paulo, Brazil. A total of 203 bats, including insectivorous, frugivorous and hematophagous species, were collected from urban and rural areas between 2020 and 2022. DNA was extracted from heart and kidney samples and analyzed using real-time PCR as a screening method, followed by conventional PCR to obtain a viable product for genetic sequencing and phylogenetic analysis. In total, 14.8 % of the samples were positive in real-time PCR and 9.4 % were sequenced. Phylogenetic analysis indicated low genetic proximity between the sequences detected in bats and those associated with domestic species, suggesting that bats may be exposed to specific sources of infection in the wild. The lack of statistical association with variables such as feeding habits and type of shelter suggests that multiple environmental factors contribute to infection. This study emphasizes the potential role of bats as intermediate hosts in the <em>T. gondii</em> transmission cycle, acting as environmental sentinels and possibly as ecological vectors. These findings contribute to understanding the distribution of <em>T. gondii</em> in bat populations and its implications for public health, especially in vulnerable communities, where poor sanitation and close contact with farm animals increase the risk of zoonotic transmission.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107557"},"PeriodicalIF":2.1,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural infection with Trypanosoma cruzi and feeding habits of Triatominae (Hemiptera: Reduviidae) from the state of Durango, Mexico","authors":"Rosa Lilián Gómez-Palacios ,&nbsp;Karina Ruiz-Tovar ,&nbsp;Herón Huerta ,&nbsp;Mario Octavio González-Reyes ,&nbsp;Irma Padilla-Medina ,&nbsp;Jorge Luis de-la-Rosa-Arana ,&nbsp;Salvador Fonseca-Coronado","doi":"10.1016/j.actatropica.2025.107555","DOIUrl":"10.1016/j.actatropica.2025.107555","url":null,"abstract":"<div><div>Chagas disease is a chronic and systemic condition caused by the protozoan <em>Trypanosoma cruzi</em>, acquired via abrasions or wounds on the skin or mucous membranes, through contact with feces or urine of hematophagous bugs belonging to the family Reduviidae, subfamily Triatominae. Many regions in Mexico are endemic for Chagas disease, but entomological and parasitological data are still limited. This study identified the incidence of natural infection with <em>T. cruzi,</em> as well as the feeding habits of the different species of triatomines circulating in the state of Durango, in the north-central region of Mexico. One hundred and ninety triatomines were taxonomically identified between February 2023 and May 2024 and a sample of intestinal content was obtained to identify the presence of <em>T. cruzi</em> by using microscopy and endpoint PCR<em>.</em> In addition, a 382 bp product of vertebrate cytochrome B (CytB) was amplified and sequenced to obtain the profile of feeding habits of this triatomines. Of the specimens collected, 121 were adults and 69 nymphs ranging from stage I to stage V. Of the collected adults, 115 were identified as <em>Triatoma longipennis</em> and 6 as <em>Triatoma recurva</em>. The specimens were collected both indoors (116) and in the peridomicile (74) showing a high overall percentage of positivity to natural infection with <em>T.cruzi</em> (54.5 %). Considering the total number of specimens collected, the colonization index was 36.32 %. In 27 of the 190 samples (14.22 %) CytB was amplified, revealing humans as the main feeding source for the triatomines. Our findings reveal that both species of triatomines were infected and predominantly found indoors, with humans as their primary feeding source. Comprehensive knowledge of the behavior of triatomine species that transmit <em>T. cruzi</em> allows their integrated control and the implementation of molecular tools for precise identification that strengthens the algorithms for epidemiological surveillance.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107555"},"PeriodicalIF":2.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics of Amblyomma ticks in South America: A meta-analytical approach","authors":"Rafael M. Nascimento ,&nbsp;Renato C. Macedo-Rego ,&nbsp;Ralph Maturano ,&nbsp;Kátia M. Famadas","doi":"10.1016/j.actatropica.2025.107552","DOIUrl":"10.1016/j.actatropica.2025.107552","url":null,"abstract":"<div><div>In this study, we carried out a detailed analysis of the seasonality of ticks of the genus <em>Amblyomma</em> in the Neotropical region by means of a meta-analysis. Our aim was to identify temporal patterns and factors that influence the seasonality of these ectoparasites, considering different developmental stages (larvae, nymphs and adults) to provide a comprehensive understanding of the population dynamics of this group. To do this, we carried out a systematic review in databases such as <em>Scopus</em> and <em>Web of Science</em>. Studies that assessed the prevalence, abundance, or seasonality of <em>Amblyomma</em> ticks in the Neotropical region were included in our data collection. Despite our focus on the Neotropics, we only obtained data on South American populations. Multilevel meta-analytical models were used to test whether larvae, nymphs and adults of <em>Amblyomma</em> ticks show higher mean abundance, mean intensity and prevalence in the dry or in the rainy season. Our results validate that <em>Amblyomma</em> larvae, nymphs, and adults show distinct patterns of seasonality. During the dry season, larvae and nymphs occur more frequently, while adults predominate in the rainy season. We also observed variations in seasonal occurrence at the species level, highlighting the complexity and variability of these patterns. Thus, in summary, the data we provide here contributes to a better understanding on the temporal distribution of these ectoparasites, as well as the factors contributing for such distribution, thus providing subsidies for the development of more effective strategies for the control and prevention of tick-borne diseases. By showing research gaps in the literature, we also argue that further research on the seasonal patterns of ticks is needed to improve our understanding of the factors that influence the population dynamics of these parasites and to implementing more effective public health measures.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107552"},"PeriodicalIF":2.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evidence of Bartonella spp. in biting midges of the genus Culicoides Latreille (Diptera: Ceratopogonidae) from Brazil","authors":"Paulo Vitor Cadina Arantes ,&nbsp;Israel de Souza Pinto ,&nbsp;Maria Clara Alves Santarém ,&nbsp;Maria Luiza Felippe-Bauer ,&nbsp;Daniel Antônio Braga Lee ,&nbsp;Rosangela Zacarias Machado ,&nbsp;Marcos Rogério André","doi":"10.1016/j.actatropica.2025.107553","DOIUrl":"10.1016/j.actatropica.2025.107553","url":null,"abstract":"<div><div><em>Bartonella</em> spp. are typical vector-borne pathogens, whose transmission has been primarily associated with a wide variety of hematophagous arthropods. These bacteria are responsible for several clinical syndromes in both humans and animals. The present study aimed to investigate the occurrence and molecular identity of <em>Bartonella</em> spp. in 345 specimens of <em>Culicoides</em> Latreille from the Brazilian Amazon. Out of 345 <em>Culicoides</em> specimens collected in the Amazon National Park, state of Pará, northern Brazil, 86.7 % (299/345) were positive for the endogenous cytochrome c oxidase subunit 1 gene. We detected a 24 % (72/299) prevalence rate for <em>Bartonella</em> spp. using a quantitative real-time PCR targeting the 16S-23S rRNA internal transcribed spacer (ITS) region. <em>Culicoides foxi</em> was statistically more associated with <em>Bartonella</em> positivity when compared to <em>C. hylas</em> and C. <em>leopoldoi. Culicoides</em> specimens collected at the ground level were more associated with positivity for <em>Bartonella</em> spp. when compared to those collected at the canopy. Further molecular characterization was performed using conventional PCR assays targeting seven molecular markers (<em>nuoG, gltA, groEL, ftsZ, rpoB, pap31</em>, and <em>ribC</em>). The BLASTn analysis revealed four <em>gltA</em> sequences with 99.72–100 % identity to <em>Bartonella bovis</em> previously detected in cattle from Brazil. One <em>ribC</em> sequence showed 100 % identity to <em>Bartonella henselae</em> previously detected in a cat from Germany. Six <em>pap31</em> sequences showed 90–91 % identity to a <em>Bartonella</em> sp. sequence previously detected in a bat (<em>Carollia perspicillata</em>) from Brazil. This study represents the first molecular evidence of <em>Bartonella</em> spp. DNA in biting midges and contributes to understanding their role in the epidemiological cycles of these agents.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107553"},"PeriodicalIF":2.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral metagenomics of hematophagous insects collected in the Carajas mining complex, Pará State, Brazil","authors":"Camila Margalho Braga ,&nbsp;Sandro Patroca da Silva ,&nbsp;Joaquim Pinto Nunes Neto ,&nbsp;Daniele Barbosa de Almeida Medeiros ,&nbsp;Ana Cecília Ribeiro Cruz ,&nbsp;Bruna Laís Sena do Nascimento ,&nbsp;Lucas Rafael Santana Pinheiro ,&nbsp;Lívia Carício Martins","doi":"10.1016/j.actatropica.2025.107551","DOIUrl":"10.1016/j.actatropica.2025.107551","url":null,"abstract":"<div><div>Hematophagous insects are vectors of viruses that cause diseases in humans and animals worldwide. Mosquitoes (<em>Culicidae</em>), biting midges (<em>Ceratopogonidae</em>), and sandflies (<em>Psychodidae</em>) were collected in three municipalities (Marabá, Canaã dos Carajás, and Curionópolis) in the state of Pará, Brazil, in 2019. Morphological keys were used for the taxonomic identification of insect species. High-throughput sequencing and metagenomic analysis were employed to characterize the viromes of the hematophagous insects. We characterized the virome of 839 insects grouped into 14 pools. A total of 729 million paired reads were generated, with 12 million viral sequences (3 % of the reads). The families <em>Reoviridae, Myoviridae, Retroviridae</em>, and <em>Poxviridae</em> were found in all samples of this study. Phylogenies of RNA-dependent RNA polymerase (RdRp) from viruses of the families <em>Chuviridae, Dicistroviridae, Flaviviridae, Iflaviridae, Mesoniviridae, Phenuiviridae,</em> and <em>Rhabdoviridae</em> were performed. In this study, the first isolation of the <em>Guaico Culex Virus</em> (GCXV) in the northern region of Brazil was obtained from a pool of <em>Culex (Melanoconion)</em> spp. mosquitoes collected in Curionópolis. The data obtained in this study demonstrate that the Carajás region has an ecosystem rich in viruses. Additional studies are needed to understand the dynamics of viruses in vectors, vertebrates, and the human population in the region.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107551"},"PeriodicalIF":2.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}