Acta tropica最新文献

{"title":"Corrigendum to \"Identification and characterization of Jingmen tick virus from Rhipicephalus microplus in Hunan, China\" [Acta Tropica 260 (2024) 107378].","authors":"Li Zhang, Wenbo Xu, Yinghua Zhao, Liyan Sui, Mingxin Song, Quan Liu","doi":"10.1016/j.actatropica.2024.107443","DOIUrl":"10.1016/j.actatropica.2024.107443","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107443"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen.","authors":"Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang","doi":"10.1016/j.actatropica.2024.107489","DOIUrl":"10.1016/j.actatropica.2024.107489","url":null,"abstract":"<p><strong>Background: </strong>Trichomonas vaginalis (T. vaginalis) is a common sexually transmitted parasite that colonizes the human urogenital tract. Programmed and precise detection of T. vaginalis is a key step in preventing and treating trichomoniasis. However, the current detection methods of T. vaginalis, including wet mount microscopy, culture, nested PCR, loop-mediated isothermal amplification, and recombinant enzyme polymerase amplification, have some shortcomings. Therefore, it is urgent to establish a programmed, sensitive, and specific method for detecting T. vaginalis.</p><p><strong>Methods: </strong>T. vaginalis cysteine protease 39 (TvCP39) was expressed in segments as TvCP39-1 and TvCP39-2, and the polyclonal antibodies were prepared by immunizing rats and rabbits. The concentration of the polyclonal antibodies of anti-rTvCP39-2 and anti-rTvCP39-1 was determined by square matrix titration. The sensitivity and specificity of double antibody sandwich ELISA were analyzed and evaluated by detecting rTvCP39 and T. vaginalis excretory-secretory proteins (TvESPs) diluted in multiple ratios and detecting excretory-secretory proteins of T. vaginalis and other pathogens, respectively. The detection efficiency of wet mount microscopy, nested PCR, and double antibody sandwich ELISA was compared by testing sixty-two clinical samples from vaginal secretions.</p><p><strong>Results: </strong>The natural TvCP39 protein could be specifically recognized by anti-rTvCP39-1 and anti-rTvCP39-2 antibodies. The concentrations of anti-rTvCP39-2 and anti-rTvCP39-1 polyclonal antibodies were determined to be 0.58 μg/mL and 0.45 μg/mL, respectively. The results of the sensitivity test showed that the detection limits of rTvCP39 and TvESPs by double antibody sandwich ELISA were 1.76 ng/mL and 107.125 μg/mL, respectively. The specificity test results showed that the double antibody sandwich ELISA had a high specificity for the detection of T. vaginalis and did not cross-react with Escherichia coli, Staphylococcus aureus, Candida albicans, and Lactobacillus. The positive detection rate of clinical samples by double antibody sandwich ELISA was higher than that by wet mount microscopy, and was the same as nested PCR. The sensitivity of double antibody sandwich ELISA was consistent with that of nested PCR. The coincidence rate between double antibody sandwich ELISA and nested PCR was 100% (Kappa=1, P < 0.001).</p><p><strong>Conclusion: </strong>The double antibody sandwich ELISA detection method for T. vaginalis established in this study had the advantages of high sensitivity and specificity, and did not require the extraction of genomic DNA. This programmatic and simple detection method was suitable for batch testing of clinical samples and exhibited the potential value in the treatment and prevention of trichomoniasis.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107489"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Techniques and Tools for Mosquito Control.","authors":"Rui-De Xue, Tong-Yan Zhao, Chun-Xiao Li","doi":"10.1016/j.actatropica.2024.107425","DOIUrl":"10.1016/j.actatropica.2024.107425","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107425"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repellent activity of icaridin-impregnated horsecloth against horse flies","authors":"Keisuke Suganuma ,&nbsp;Go Fujita ,&nbsp;Adrian Miki C. Macalanda ,&nbsp;Maria Angenica F. Regilme ,&nbsp;Hiroshi Izumida ,&nbsp;Noboru Inoue ,&nbsp;Tomas J. Acosta","doi":"10.1016/j.actatropica.2024.107485","DOIUrl":"10.1016/j.actatropica.2024.107485","url":null,"abstract":"<div><div>Horseflies are pests that cause discomfort from blood-sucking and disease transmission, and economic losses in the equine industry. This study evaluated the efficacy of horsecloth impregnated with icaridin in reducing horsefly attacks and deterring horseflies. Repellent activities were evaluated under three conditions: treatment 1 (no horsecloth), 2 (horsecloth without icaridin), and 3 (horsecloth impregnated with icaridin), using three native Hokkaido horses (Dosanko) and three mixed-breed horses (Dosanko and Haflinger) in July 2023 at a riding horse club in Hokkaido, Japan. Treatment 3 significantly reduced the number of horseflies. Treatment 2 did not significantly reduce horsefly numbers. Treatments 2 and 3 significantly reduced the number of avoidance actions. The reduction in avoidance actions in treatment 3 was greater than that in treatment 2. Lighter-colored horses experienced fewer fly attacks and avoidance actions than darker-colored horses. Overall, using icaridin-impregnated horsecloths (treatment 3) was more effective for repelling horseflies than the use of physical barriers alone (treatment 2). This study suggests that integrating chemical repellents with physical protection can enhance horsefly control strategies, improve horse welfare, and improve the safety of horses interacting with them. Further research is recommended to assess the generalizability of these findings to different horse breeds and environments with a greater number of horses.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107485"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence of endogenous non-retroviral RNA virus sequences into the genome and transcriptome of the malaria vector Anopheles darlingi","authors":"Juan C. Hernandez-Valencia ,&nbsp;Paola Muñoz-Laiton ,&nbsp;Giovan F. Gómez ,&nbsp;Margarita M. Correa","doi":"10.1016/j.actatropica.2024.107469","DOIUrl":"10.1016/j.actatropica.2024.107469","url":null,"abstract":"<div><div>The characterization of non-retroviral integrated RNA virus sequences (NIRVS) in mosquitoes has emerged as a significant area of research that could yield insight into virus-host interactions. This study aimed to characterize NIRVS in the <em>Anopheles darlingi</em> reference genome and identify putative transcribed NIRVS in field-collected mosquitoes from Colombia. The <em>An. darlingi</em> reference genome was analyzed to identify and characterize NIRVS by conducting a BLAST query with all the virus sequences previously identified in arthropods available in the NCBI-virus repository. In addition, <em>An. darlingi</em> field-collected mosquitoes were examined for NIRVS using a metatranscriptomic approach. As a result, 44 NIRVS were identified in the <em>An. darlingi</em> genome, constituting integrations of negative single-stranded RNA viruses (ssRNA-) from the families <em>Rhabdoviridae, Chuviridae</em> and <em>Phasmaviridae</em>, and integrations of double-stranded RNA viruses (dsRNA) from the families <em>Partitiviridae</em> and <em>Sedoreoviridae</em>. These NIRVS were not randomly distributed but clustered in specific regions of the genome enriched with BEL/Pao and Ty3/Gypsy long terminal repeat elements. Furthermore, putative NIRVS-like sequences were present in the transcriptomic data from all the Colombian <em>An. darlingi</em> natural populations. This study is significant as it represents the first identification of NIRVS in the most important malaria vector of the Neotropics. The findings help in understanding the intricate relationship between the mosquito and its virome, and the regulation of viruses’ mechanisms in the <em>Anopheles</em> genus.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107469"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and age-period-cohort effect on incidence of brucellosis from 2006 to 2020 in China.","authors":"Weihao Li, Hanqi Ouyang, Ziyu Zhao, Liying Wang, Weiwei Meng, Sanji Zhou, Guojing Yang","doi":"10.1016/j.actatropica.2024.107475","DOIUrl":"10.1016/j.actatropica.2024.107475","url":null,"abstract":"<p><p>Brucellosis remains a major public health challenge in China and globally. This study analyzed long-term trends in brucellosis incidence in China from 2006 to 2020, assessed the effects of age, period, and birth cohort, and projected future incidence up to 2030. Data on brucellosis were obtained from the Data-center of China Public Health Science, and temporal trends in incidence rates were analyzed using joinpoint regression, while an age-period-cohort model evaluated the effects of age, period, and cohort. A Bayesian age-period-cohort model was applied for future projections. From 2006 to 2020, 586,371 brucellosis cases were reported, with an upward trend in age-standardized incidence rates for both males and females, showing average annual percent changes of 3.37 % and 4.61 %, respectively. The age-period-cohort model revealed that age, period, and cohort all influenced incidence, with males facing higher rates. High-risk groups were identified among those aged 50-84, particularly in the 65-69 age range, where incidence was highest and showed the most significant annual increase. Period risk initially rose then declined, while later-born cohorts had higher risks. Projections indicate a continued rise in brucellosis incidence. Targeted prevention and control measures are recommended, especially for older adults and males.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107475"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction of Chlamydia psittaci into a hospital area by feral pigeons","authors":"Ricardo Lustosa ,&nbsp;Maria Catalina Ospina-Pinto ,&nbsp;Tânia Barros ,&nbsp;Pedro Cerqueira Lima ,&nbsp;Carlos Roberto Franke ,&nbsp;Tânia Freitas Raso","doi":"10.1016/j.actatropica.2024.107479","DOIUrl":"10.1016/j.actatropica.2024.107479","url":null,"abstract":"<div><div>Pigeons are associated with zoonotic pathogens such as <em>Chlamydia psittaci</em>, the main causative agent of avian chlamydiosis, and related to psittacosis cases in humans worldwide. The aim of the present study was to investigate the occurrence of <em>C. psittaci</em> in feral pigeons (<em>Columba livia</em>) and environmental samples from places frequented by pigeons in a Brazilian hospital area. A cross-sectional study was carried out sampling feral pigeons, their droppings and nest material in a hospital area. Squares in a nearby region with a high density of pigeons were also sampled. Pigeon cloacal swabs (n=123) were collected from each bird, as well as pigeon droppings from the environment (n=77) and material from pigeon's nests (n=28). <em>Chlamydiaceae</em>-PCR targeting the 23S rRNA gene was used as screening. Positive samples were submitted to another PCR targeting the <em>omp</em>A gene of <em>C. psittaci</em>, followed by sequencing and phylogenetic analysis. <em>C. psittaci</em> was detected in 7.5% (17/228) of the samples, 7.3% (12/164) from the hospital area and 7.8% (5/64) from the squares. By sample type, 9.8% (12/123) of the pigeon cloacal swabs, 5.2% of droppings (4/77) and 3.6% of nest material (1/28) were positive for <em>C. psittaci</em>. All sequenced samples corresponded to <em>C. psittaci</em> genotype B. These results demonstrate the occurrence of <em>C. psittaci</em> in urban areas, with emphasis on a hospital area where immunocompromised individuals are present. Adopting a One health approach to prevent the proliferation of the pigeons, health education campaigns and specific recommendations for the hospital administration are essential. Guidance on practices such as not feeding the pigeons, avoiding contact or proximity and removing available areas for roosting and nesting, are measures that will reduce environmental contamination and risk of human exposure to <em>C. psittaci</em>.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107479"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mosquito diversity, abundance and phenology in Mexico City: A heavily urbanized environment","authors":"Stephany Rodríguez-González ,&nbsp;Daniel Sánchez-Ochoa ,&nbsp;Herón Huerta ,&nbsp;Manuel Edday Farfán-Beltrán ,&nbsp;Alex Córdoba-Aguilar","doi":"10.1016/j.actatropica.2024.107484","DOIUrl":"10.1016/j.actatropica.2024.107484","url":null,"abstract":"<div><div>We need to understand how zoonotic diseases make their way in urbanized areas, and one way is via gathering well-structured temporal and spatial accounts of zoonotic disease vectors. Due to its large biotic and abiotic heterogeneity, Mexico City provides favorable environmental conditions for the proliferation of mosquitoes including vector species of medical importance. Hereby we present the results of one year sampling (May 2023-April 2024) in eight contrasting locations in Mexico City. In total, 4,861 mosquitoes belonging to 11 species were captured, with <em>Culex stigmatosoma</em> and <em>Culex restuans</em> emerging as the most dominant, while <em>Anopheles aztecus, Culex erythrothorax</em> and <em>Aedes trivittatus</em> were the least abundant. Monthly fluctuations in the composition and abundance of species were observed at each site, highlighting cemeteries as the places with the highest mosquito density, making them key areas for monitoring. This research should serve local health authorities to guide mosquito control strategies, and two key candidates are <em>Culex quinquefasciatus</em> and <em>Cx. restuans</em>, particularly from June to November which is when their abundance reaches a peak.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107484"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype","authors":"Anna Claudia Baumel Mongruel ,&nbsp;Emília Patrícia Medici ,&nbsp;Ariel Costa Canena ,&nbsp;Amir Salvador Alabi Cordova ,&nbsp;Lorena Freitas das Neves ,&nbsp;Eliz de Oliveira Franco ,&nbsp;Rosangela Zacarias Machado ,&nbsp;Marcos Rogério André","doi":"10.1016/j.actatropica.2024.107476","DOIUrl":"10.1016/j.actatropica.2024.107476","url":null,"abstract":"<div><div>Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (<em>Tapirus terrestris),</em> the largest land mammals in Brazil, are scarce. The aim of the present study was to investigate the occurrence and molecular identity of <em>Anaplasmataceae</em> agents, <em>Coxiella burnetii</em> and <em>Hepatozoon</em> spp. in blood samples of wild <em>T. terrestris</em> from two biomes (Cerrado and Pantanal) in Brazil. A total of 122 blood samples from 99 tapirs were analyzed. Sixty-one tapirs were sampled in Pantanal, whereas 38 were from Cerrado biome. DNA was extracted from blood samples and subjected to conventional and/or quantitative PCR assays for molecular screening and characterization of DNA from <em>Anaplasmataceae</em> agents (<em>Anaplasma, Ehrlichia</em>, and <em>Neorickettsia</em>), <em>C. burnetii</em> and <em>Hepatozoon</em> spp. None of the samples were positive for <em>Ehrlichia, C. burnetii</em> or <em>Hepatozoon</em> spp. Twenty-two samples (22/122; 18%) amplified fragments from the expected size for the <em>Anaplasma</em> 16S rRNA fragment tested herein. Out of these samples, 2 (9.1%) presented amplification for the <em>Anaplasma</em> ITS 23S-5S. Nine positive samples for the 16S rRNA assay were selected for cloning and sequencing. Phylogenetically, distance and haplotype analyses based on large fragments (&gt;1,200 bp) of the 16S rRNA suggest that tapir-related <em>Anaplasma</em> and <em>Anaplasma odocoilei</em> are genetically similar species. Moreover, 31 (25.4%) samples were positive for <em>Neorickettsia</em> based on amplification of partial 16S rRNA. Phylogenetic assessment of the three obtained sequences demonstrated relatedness to <em>Neorickettsia risticii</em>, the causative of Potomac fever in horses. This is the first report of <em>Neorickettsia</em> sp. and description of a new <em>Anaplasma</em> genotype in tapirs.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107476"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142742947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial prediction of human brucellosis susceptibility using an explainable optimized adaptive neuro fuzzy inference system.","authors":"Ali Jafari, Ali Asghar Alesheikh, Iman Zandi, Aynaz Lotfata","doi":"10.1016/j.actatropica.2024.107483","DOIUrl":"10.1016/j.actatropica.2024.107483","url":null,"abstract":"<p><p>Brucellosis, a zoonotic disease caused by Brucella bacteria, poses significant risks to human, livestock, and wildlife health, alongside economic losses from livestock morbidity and mortality. This study improves Human Brucellosis Susceptibility Mapping (HBSM) by integrating the Adaptive Neuro-Fuzzy Inference System (ANFIS) with meta-heuristic algorithms, including Genetic Algorithm (GA) and Particle Swarm Optimization (PSO). Boruta-XGBoost identified key covariates, while VIF and tolerance tests addressed collinearity, and Shapley additive explanation (SHAP) values enhanced model interpretability. In Mazandaran province, Iran (2012-2018), the hybrid ANFIS-PSO model demonstrated superior performance (RMSE: 0.5076; R²: 0.6980). SHAP analysis highlighted mean elevation, NDVI, and relative humidity as the most impactful covariates, while max evaporation and precipitation had minimal influence. ANFIS-based models outperformed Support Vector Regression (SVR), offering a robust framework for brucellosis control. This approach enables effective interventions and resource allocation, with potential for improvement through advanced algorithms and greater interpretability.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107483"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}