Acta tropica最新文献

{"title":"Improved antiparasitic effects of mebendazole using chitosan and zinc oxide nanocomposites for drug delivery in Trichinella spiralis infected mice during the muscular phase","authors":"Nabila M. Mira ,&nbsp;Aya M. Henaish ,&nbsp;Eman A. Moussa ,&nbsp;Ibrahim B. Helal ,&nbsp;Shaimaa M. Kasem","doi":"10.1016/j.actatropica.2025.107565","DOIUrl":"10.1016/j.actatropica.2025.107565","url":null,"abstract":"<div><div>This study was performed to assess the use of chitosan (Cs) and zinc oxide nanocomposites ZnO NCP with full and half dose mebendazole (MBZ) during the muscular phases of <em>Trichinella spiralis</em> infection. Sixty Swiss Albino male mice were divided into six groups: G1 (negative control), G2 (positive control), G3 (MBZ at 200 mg/kg/day), G4 (Cs@MBZ NCP at 400 mg/kg/day), G5 ([email protected] NCP), and G6 ([email protected] NCP). Mice were infected orally with 200 <em>T. spiralis</em> larvae and received treatments starting on day 35 post-infection for five consecutive days. Treatment outcomes were evaluated by counting total muscular larvae, conducting blood biochemical measurements, and performing histopathological examinations of the liver and hip joint muscles. ELISA was used to measure serum levels of transforming growth factor-beta1 (TGF-β1), tumor necrosis factor-alpha (TNF-α), and vascular endothelial growth factor (VEGF). Results indicated that both [email protected] NCP and [email protected] NCP groups exhibited significant reductions in muscular larval counts (96.4 % and 96.1 %, respectively). Treated mice also showed reduced AST and ALT levels, increased total protein and albumin, and decreased globulin levels compared to positive controls. Cytokines levels of TNF-α, TGF-β1, and VEGF were lower in treated groups. Histopathological examination revealed that [email protected] and [email protected] NCP restored up to 90 % of normal tissue architecture. In conclusion, chitosan and zinc oxide nanocomposites enhanced the therapeutic ability of mebendazole against <em>T. spiralis</em> muscular stage as these nanocomposites had the highest effect on reducing parasite burden, improving blood biochemical, decreasing cytokines levels and restoring normal histological architecture.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107565"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the immunogenic potential of recombinant Mycobacterium bovis BCG expressing the ASP-2 and TC24 proteins from Trypanosoma cruzi","authors":"Guilherme Senna dos Santos ,&nbsp;Bárbara da Rocha Fonseca ,&nbsp;Fernanda Severo Sabedra Sousa ,&nbsp;Fabiana Kommling Seixas ,&nbsp;Sibele Borsuk","doi":"10.1016/j.actatropica.2025.107569","DOIUrl":"10.1016/j.actatropica.2025.107569","url":null,"abstract":"<div><div>Although drugs have been employed over the past years to treat Chagas disease, they work mostly on the acute phase of infection, where diagnosis is hardly ever made, and present a plethora of negative side effects that ends in discontinuation of treatment. Consequently, to deal with this disease, prophylaxis seems to be a better strategy, with recombinant subunit vaccines showing promising results. Among those, <em>Mycobaterium bovis</em> Bacillus Calmette-Guérin (BCG) has recently been employed as vector for delivering <em>T. cruzi</em> antigens with positive results on immune response stimulus and protection against the infection. Following this perspective, this study aimed to characterize the immune response elicited by recombinant BCG expressing a fraction of the amastigote surface protein 2 (ASP-2) and the 24 kDa flagellar calcium-binding protein (TC24) of <em>T. cruzi</em>. To accomplish this, four groups of BALB/c female mice (<em>n</em> = 10) were vaccinated with 0.9% saline solution (Group 1), non-transformed BCG Pasteur (Group 2), rBCG/pUS977/ASP-2 (Group 3) or rBCG/pUS977/TC24 (Group 4). Cellular responses, assessed by cytokine expression from cultured and protein stimulated splenocytes, were statistically higher for both vaccinal formulations when compared with basal levels (Group 1) and non-transformed BCG (Group 2). Group 3 achieved better results for interleukins 10 and 17, while interferon γ was greatly stimulated by vaccination with Group 4. Even though further analyses are needed to evaluate the full efficacy of the constructions, the here presented results exhibit the potential of BCG vectored vaccines in eliciting Th1/Th2/Th17 mixed immune responses.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107569"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Period of time and movement distances between feeding and postfeeding defecation in Triatoma pallidipennis (Heteroptera: Reduviidae)","authors":"Benjamín Nogueda-Torres ,&nbsp;Fernando Cervantes-Hernández ,&nbsp;Yunuen Grant-Guillén ,&nbsp;J. Guadalupe Michel-Parra ,&nbsp;José Alejandro Martínez-Ibarra","doi":"10.1016/j.actatropica.2025.107563","DOIUrl":"10.1016/j.actatropica.2025.107563","url":null,"abstract":"<div><div>Defecation time is one of the key parameters used to estimate the efficiency of a Triatomine species as a vector of <em>Trypanosoma cruzi</em>. There is controversy among experts as to whether triatomines that defecate 1–10 min after feeding are efficient vectors, with a different perspective on the distance moved from the host to defecate in the case of triatomines after feeding. The aim of the present study was to determine the period of time and movement distances between feeding and defecation of third-, fourth- and fifth- nymphal instars and adults of <em>Triatoma pallidipennis</em> specimens that defecated between 1 and 10 min and to estimate the distance where the host could still be in contact with deposited feces and potentially become infected with <em>T. cruzi</em>. Twelve drops of non-toxic coloring were deposited to simulate triatomine feces close (&lt; 10 cm) to 20 dogs, and their movements were observed to estimate the chances of becoming “infected”. The mean postfeeding defecation delay was between 2 and 10 min in the five studied instars, with significantly (<em>p</em> = 0.03) longer times in the fourth-instar nymphs and males. The mean distance moved after feeding until defecation was ranged from 5.3 cm in third-instar nymphs to 19.6 cm in males, with significant differences (<em>p</em> &lt; 0.01) only in males. All the studied dogs had accidental contact with at least three drops (mean of five drops) during their movements. <em>Triatoma pallidipennis</em> remains at its host for only a few seconds postfeeding; however, it defecates close enough to a host to expose it to its feces due to host movement. According to the results obtained, the delay should be complemented by the observation of distance moved to defecate to more accurately estimate the efficiency as a vector of <em>T. cruzi</em> of a triatomine species.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107563"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The proteome of human adult whipworm Trichuris trichiura: A source of potential immunomodulatory molecules","authors":"Leonardo F. Santiago ,&nbsp;Eduardo S. da Silva ,&nbsp;Priscila S. dos Santos ,&nbsp;Luis F. Salazar- Garcés ,&nbsp;Sara P.O. Santos ,&nbsp;Antônio M.S. Fernandes ,&nbsp;Raphael C. Silva ,&nbsp;Vitor S. Alves ,&nbsp;Peter Briza ,&nbsp;Fatima Ferreira ,&nbsp;Luis G.C. Pacheco ,&nbsp;Neuza M. Alcantara-Neves ,&nbsp;Carina S. Pinheiro","doi":"10.1016/j.actatropica.2025.107566","DOIUrl":"10.1016/j.actatropica.2025.107566","url":null,"abstract":"<div><div>Soil-transmitted helminths (STHs), including <em>Trichuris trichiura</em>, pose a major global health challenge, particularly in children, causing significant morbidity. However, <em>T. trichiura</em>'s ability to modulate host immune responses offers a unique opportunity to discover biomolecules with therapeutic potential for inflammatory, allergic, and autoimmune disorders. This study conducted a proteomic analysis of adult male and female <em>T. trichiura</em> using liquid chromatography-tandem mass spectrometry (LC-MS/MS), identifying 810 parasite proteins. Of these, 177 were exclusive to females, 277 to males, and 356 shared.</div><div>Gene ontology analysis showed similar cellular component profiles in males and females, mostly involving intracellular structures. However, female-exclusive proteins exhibited more diverse components. Molecular function analysis highlighted hydrolytic and catalytic activities, suggesting enzymatic strategies for nutrition and immune modulation. Notably, immunomodulatory proteins were identified in both sexes, showing therapeutic potential, including a Kunitz protease inhibitor and glutamate dehydrogenase.</div><div>To evaluate immunomodulatory properties, one identified protein (rc4299) was tested on cultures of peripheral blood mononuclear cells (PBMCs) from allergic individuals. The recombinantly produced rc4299 increased IL-10 secretion, indicating potential for treating autoimmune and allergic diseases. This study uncovers the <em>T. trichiura</em> proteome and highlights promising therapeutic targets, emphasizing the parasite's complex interactions with the host immune system.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107566"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the immunogenicity of elephant endotheliotropic herpesvirus glycoprotein B (EEHV-gB) subunit vaccines in a mouse model","authors":"Tidaratt Sittisak ,&nbsp;Thunyamas Guntawang ,&nbsp;Saralee Srivorakul ,&nbsp;Kornravee Photichai ,&nbsp;Anucha Muenthaisong ,&nbsp;Amarin Rittipornlertrak ,&nbsp;Varankpicha Kochagul ,&nbsp;Naricha Khamluang ,&nbsp;Nattawooti Sthitmatee ,&nbsp;Phongsakorn Chuammitri ,&nbsp;Chatchote Thitaram ,&nbsp;Wei-Li Hsu ,&nbsp;Kidsadagon Pringproa","doi":"10.1016/j.actatropica.2025.107571","DOIUrl":"10.1016/j.actatropica.2025.107571","url":null,"abstract":"<div><div>Elephant endotheliotropic herpesvirus (EEHV), a persistent threat, has caused significant mortality among young Asian elephants (<em>Elephas maximus</em>), raising serious concerns for the conservation of this endangered species. Given the urgent need for protective measures, research into EEHV vaccine development has become increasingly critical. This study evaluated the immune response in mice following immunization with an EEHV1A-glycoprotein B (gB) subunit vaccine. The vaccine incorporated gBF1 and gBF2, corresponding to segments of the gB ectodomains I and IV, respectively, along with emulsion adjuvants Montanide™ ISA 206 VG and incomplete Freund's adjuvant (IFA). The findings revealed that both gBF1 and gBF2, when paired with these adjuvants, were capable of inducing strong humoral immune responses against EEHV-gB, as demonstrated by the ability of sera from immunized mice to detect EEHV-gB <em>ex vivo</em>. Additionally, in terms of cellular immunity, the vaccine formulations predominantly activated CD4+ T cells, including both Th1 (IFN-γ+) and Th2 (IL-4+) cells, with no significant activation of CD8+ T cells. If also applicable in elephants, gB-based vaccines would be a significant step forward in the fight against EEHV.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107571"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential impact of climate change on Opisthorchis viverrini and Opisthorchis felineus transmission in Eurasia","authors":"Banchob Sripa ,&nbsp;Natalia Yurlova ,&nbsp;Apiporn T. Suwannatrai ,&nbsp;Elena Serbina ,&nbsp;Sirikachorn Tangkawattana ,&nbsp;Somphou Sayasone ,&nbsp;Pariwate Varnakovida","doi":"10.1016/j.actatropica.2025.107574","DOIUrl":"10.1016/j.actatropica.2025.107574","url":null,"abstract":"<div><div>Human liver flukes of the family Opisthorchiidae, particularly <em>Opisthorchis viverrini</em> and <em>Opisthorchis felineus</em>, are major foodborne trematode parasites endemic to the Lower Mekong River Basin (Southeast Asia) and the Ob-Irtysh Basins (southern Western Siberia, Russian Federation), respectively. Together, these parasites infect over 14 million people, with an estimated 300 million at risk. Their transmission cycles involve <em>Bithynia</em> snails and Cyprinidae fish as first and second intermediate hosts, respectively, with humans and other fish-eating mammals serving as definitive hosts. The geographical distribution of these flukes is shaped by specific <em>Bithynia</em> species: <em>B. siamensis, B.</em> s<em>. goniomphalos</em>, and <em>B. funiculata</em> for <em>O. viverrini</em>, and <em>B. troschelii, B. leachi</em>, and <em>B. inflata</em> for <em>O. felineus</em>. Climate change directly influences liver fluke transmission by affecting parasite survival, host biology, and environmental conditions. <em>Bithynia</em> snails are highly temperature-sensitive, and <em>O. viverrini</em> transmission is notably temperature-dependent, with a 1 °C increase raising infection odds by 5.4 %. Temperatures exceeding 30 °C reduce cercarial survival and infectivity. In Western Siberia, favorable water temperatures for O. felineus transmission start at +15 °C, with higher temperatures leading to an increased infection rate in snails while permafrost regions lack <em>Bithynia</em> snails entirely. Projections from the Intergovernmental Panel on Climate Change (IPCC) indicate that warming will exceed the global average in northern Asia (affecting <em>O. felineus</em>) and approach the global average in Southeast Asia (impacting <em>O. viverrini</em>). These trends suggest that climate change may have a more pronounced impact on <em>O. felineus</em> transmission in Siberia than on <em>O. viverrini</em> transmission in Southeast Asia. This review provides an in-depth discussion of <em>Bithynia</em> biology and the effects of temperature on snail growth, cercarial release, survival, infection, and aestivation, emphasizing how global warming could influence the transmission dynamics of these liver flukes.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107574"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Evaluation of Wolbachia infection in Aedes aegypti suggests low prevalence and highly heterogeneous distribution in Medellín, Colombia” [Acta Tropica, Volume 260, December 2024, 107423]","authors":"Arley Calle-Tobón ,&nbsp;Raúl Rojo-Ospina ,&nbsp;Sara Zuluaga ,&nbsp;Juan F. Giraldo-Muñoz ,&nbsp;Jorge Mario Cadavid","doi":"10.1016/j.actatropica.2025.107572","DOIUrl":"10.1016/j.actatropica.2025.107572","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107572"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic toxoplasmosis induces depression-like behaviors and neuroinflammatory responses in mice","authors":"Wen-Qian Shi ,&nbsp;Shao-Yuan Bai ,&nbsp;Ming Pan ,&nbsp;Qi-Wang Jin ,&nbsp;Zuohua Liu ,&nbsp;Xinwen Bo ,&nbsp;Si-Yang Huang","doi":"10.1016/j.actatropica.2025.107568","DOIUrl":"10.1016/j.actatropica.2025.107568","url":null,"abstract":"<div><div>Approximately one-third of the global population is infected with <em>Toxoplasma gondii</em>, which can lead to chronic infection and induce a persistent inflammatory response. This inflammation is known to increase the risk of depression-like behaviors. To investigate the impact of chronic toxoplasmosis on depression, a model of chronic infection was established, and behavioral changes were evaluated using a series of tests, including the Tail Suspension Test (TST), Sucrose Preference Test (SPT), Olfactory Sensitivity Test (OST), and Tail Immersion Test (TIT). The results showed that chronically infected mice exhibited significant depression-like behaviors and impaired olfactory sensitivity, while their pain perception remained unaffected. Additionally, the levels of astrocytes and neuroinflammation in the mouse brain were further assessed. It was observed that the expression of GFAP and proinflammatory cytokines (TNF-α, IL-1β, IL-6, IDO) was upregulated. These findings suggest that chronic toxoplasmosis induces neuroinflammation and astrocyte activation, which may contribute to the manifestation of depression-like behaviors.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"264 ","pages":"Article 107568"},"PeriodicalIF":2.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of meteorological and socio-economic factors on malaria in mainland China (2006–2015): A study using spatiotemporal analysis and Bayesian model","authors":"Lili Liu ,&nbsp;Zhenyi Wang ,&nbsp;Yanni Tian ,&nbsp;Yiwen Tao ,&nbsp;Yazhi Li","doi":"10.1016/j.actatropica.2025.107554","DOIUrl":"10.1016/j.actatropica.2025.107554","url":null,"abstract":"<div><div>Nowadays malaria still is a significant and prevalent infectious disease in mainland China. How meteorological and socio-economic factors affect the distribution of malaria in mainland China? It is worthy to explore their impact on malaria to guide other serious regions. This study selects the total number of malaria cases included in both local and imported cases of malaria in mainland China from 2006 to 2015. According to integrating 6 meteorological and socio-economic factors, we use the spatiotemporal analysis and Bayesian model to quantify the impact of multi-factors on malaria. The results show that malaria is distributed in a northeast southwest direction, with hotspots mainly located in Shandong, Anhui, Jiangsu etc. Precipitation has a significant impact on the incidence of malaria, while socio-economic factors have no significant impact on the incidence of malaria. In addition, malaria in mainland China exhibits significant spatial heterogeneity, with significant differences in <span><math><mrow><mi>R</mi><mi>R</mi></mrow></math></span>(Relative Risk) of different regions. The related results aim to provide the theoretical guidance for formulating the prevention and control policy.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107554"},"PeriodicalIF":2.1,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA barcoding and host blood meal identification of Culicoides Latreille (Diptera, Ceratopogonidae) from Malaysia","authors":"Zubaidah Ya'cob ,&nbsp;Ronnalit Mintara ,&nbsp;Daicus M. Belabut ,&nbsp;Muhammed Rasul Abdullah Halim ,&nbsp;Pairot Pramual","doi":"10.1016/j.actatropica.2025.107564","DOIUrl":"10.1016/j.actatropica.2025.107564","url":null,"abstract":"<div><div>Mitochondrial cytochrome oxidase I (COI) sequences were used to determine the genetic diversity and species identification efficacy of <em>Culicoides</em> from Malaysia. In total, 100 COI sequences were obtained from 13 morphologically identified species. Intraspecific genetic divergence varied from 0.48 % in <em>C. parahumeralis</em> to 14.88 % in <em>C. palpifer</em>. However, most (8 of 13) had low (&lt;3 %) intraspecific genetic divergence. Identification of these species based on best match (BM) and best close match (BCM) methods revealed a high efficiency of the COI sequences with 100 % and 99 % success for BM and BCM, respectively. Identification in the BOLD database revealed that 10 species were successfully determined and agreed with morphological identifications. There remained three taxa which were ambiguous (<em>C. jacobsoni</em>) or had no species level identity match (<em>C. palpifer</em> and <em>C. flavescens</em>). Phylogenetic analyses found that all Malaysian specimens were clustered with conspecifics except <em>C. palpifer</em>. Specimens of this taxon separated into two divergent clades, one with members of a BIN (BOLD:ADT9601) of <em>C. palpifer</em> whereas the other formed a novel genetic lineage. Molecular species delimitation identified the morphologically identified species of the Malaysian <em>Culicoides</em> in the respective species. The exceptions were two divergent lineages found in <em>C. palpifer</em> because they were treated as two different taxa. Molecular identification of the host blood meal source revealed that all were from water buffalo (<em>Bubalus bubalis</em>).</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107564"},"PeriodicalIF":2.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}