Serological evidence of epizootic hemorrhagic disease and serotypes of epizootic hemorrhagic disease virus in Pakistan

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-05-25 DOI:10.1016/j.actatropica.2025.107675

Muhammad Ishaq , Syed M. Jamal , Liana Teodori , Alessandra Leone , Barbara Bonfini , Massimo Spedicato , Giovanni Savini

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Abstract

This study investigated seroprevalence and serotypes of Epizootic hemorrhagic disease virus (EHDV) in ruminants in North-Western Pakistan. A total of 616 serum samples were tested for the presence of antibodies against EHDV and neutralizing antibodies against 7 EHDV serotypes (EHDV-1, 2, 4, 5, 6, 7 and 8) using competitive ELISA and VNT, respectively. The overall seroprevalence of EHDV was found to be 35.9 %. Seroprevalence of EHDV in cattle, buffalo and goats was found as 66.9 %, 21.3 % and 4.1 %, respectively. In multivariate analysis, the seroprevalence of EHDV was found 12.8 (95 % CI = 6.1–27) times higher in cattle than buffalo and 3.3 (95 % CI = 1.4–10) times higher in buffalo than goats. The seroprevalence was found to be 6 (95 % CI = 3.2–11.3) times higher in old animals than in young ones. The seroprevalence was recorded as 4.8 (95 % CI = 2.3–10) and 2.1 (95 % CI = 1.3–3.6) times higher in the Southern and Central zones, respectively than in the Northern zone of the study area. Neutralizing antibodies against six different serotypes of EHDV (EHDV-1, 2, 5, 6, 7 and 8) were detected. Neutralizing antibodies against EHDV-6 was found to be the most prevalent (62.1 %) and those against EHDV-1 as the least prevalent (11.2 %). This study shows that Epizootic hemorrhagic disease (EHD) is prevalent in Pakistan and at least six different serotypes of EHDV have circulated or are circulating in the country. Virological studies are required to investigate the currently circulating serotypes of EHDV in the country.

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巴基斯坦流行性出血热的血清学证据和流行性出血热病毒的血清型。

本研究调查了巴基斯坦西北部反刍动物流行性出血病病毒（EHDV）的血清流行率和血清型。采用竞争性ELISA和VNT分别检测616份血清样本中EHDV抗体和7种EHDV血清型（ehdv1、2、4、5、6、7和8）的中和抗体。EHDV血清总阳性率为35.9%。牛、水牛和山羊EHDV血清阳性率分别为66.9、21.3和4.1%。在多变量分析中，发现牛的EHDV血清患病率是水牛的12.8倍（95% CI = 6.1-27），是山羊的3.3倍（95% CI = 1.4-10）。发现老年动物的血清患病率是年轻动物的6倍（95% CI = 3.2-11.3）。南部和中部地区的血清患病率分别是研究区北部地区的4.8倍（95% CI = 2.3-10）和2.1倍（95% CI = 1.3-3.6）。检测到6种不同血清型EHDV （EHDV-1、2、5、6、7和8）的中和抗体。edhv -6中和抗体最多（62.1%），edhv -1中和抗体最少（11.2%）。这项研究表明，动物流行性出血病（EHD）在巴基斯坦流行，至少有六种不同的EHDV血清型已经或正在该国流行。需要进行病毒学研究，以调查该国目前流行的EHDV血清型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.