Advanced biology最新文献_第7页

Flow Cytometry and Gene Expression Modulation by Euphorbia rigida Methanol Extract in A549 Lung Cancer Cells: Induction of Apoptosis Through Bax, Caspase-9, and Bcl-2 Pathways 大大麻甲醇提取物对A549肺癌细胞的流式细胞术和基因表达调控：通过Bax、Caspase-9和Bcl-2途径诱导凋亡

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-05-28 DOI: 10.1002/adbi.202500136

Zeynep Betül Sarı, Muhammed Emin Sarı, Erdi Can Aytar, Abidin Gümrükçüoğlu, Emine Incilay Torunoğlu, Tuba Ozdemir-Sanci, Gamze Demirel, Alper Durmaz

{"title":"Flow Cytometry and Gene Expression Modulation by Euphorbia rigida Methanol Extract in A549 Lung Cancer Cells: Induction of Apoptosis Through Bax, Caspase-9, and Bcl-2 Pathways","authors":"Zeynep Betül Sarı, Muhammed Emin Sarı, Erdi Can Aytar, Abidin Gümrükçüoğlu, Emine Incilay Torunoğlu, Tuba Ozdemir-Sanci, Gamze Demirel, Alper Durmaz","doi":"10.1002/adbi.202500136","DOIUrl":"10.1002/adbi.202500136","url":null,"abstract":"Non-small cell lung cancer (NSCLC) remains a major cause of cancer-related mortality. This study investigates the cytotoxic effects of Euphorbia rigida extract on A549 NSCLC cells and its potential as a therapeutic agent. Cellular morphology was observed microscopically, and cell viability was evaluated using dose-dependent proliferation assays. Apoptosis-related gene expression—including Bax, Bcl-2, and Caspase-9—was analyzed via quantitative PCR (qPCR). Chromatographic methods identified bioactive flavonoids, and molecular docking assessed their binding to cancer-related proteins. Additionally, absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles were evaluated. The extract induced apoptotic morphological changes such as cell shrinkage and loss of intercellular contact. A dose-dependent reduction in A549 viability was observed, with an IC50 of 0.5 mg mL−1. Gene expression indicated activation of the intrinsic mitochondrial apoptotic pathway, with increased Bax and Caspase-9 and decreased Bcl-2 expression. Flow cytometry using Annexin V-allophycocyanin (V-APC) staining revealed selective cytotoxicity: significant apoptosis in A549 cells while preserving viability in BEAS-2B normal lung epithelial cells. Identified flavonoids included quercetin, apigenin, and myricetin, which showed strong binding affinities in docking studies. ADMET profiling supported their drug-likeness. These findings highlight E. rigida potential in NSCLC treatment via apoptosis induction and selective cytotoxicity.\u0000","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guilongwan Ameliorates Experimental Diabetic Foot Ulcer in Rats via the Inhibition of Delta-Like 4/Notch1 Signaling in M1 Macrophages 桂龙丸通过抑制M1巨噬细胞中delta -样4/Notch1信号通路改善实验性糖尿病足溃疡大鼠

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-05-19 DOI: 10.1002/adbi.202400819

Xi-Ting Lv, Wen-Xiu Yang, Xiao Zhang, Bao-Ling Li, Wen-Ying Wang, Guo-En Wang

{"title":"Guilongwan Ameliorates Experimental Diabetic Foot Ulcer in Rats via the Inhibition of Delta-Like 4/Notch1 Signaling in M1 Macrophages","authors":"Xi-Ting Lv, Wen-Xiu Yang, Xiao Zhang, Bao-Ling Li, Wen-Ying Wang, Guo-En Wang","doi":"10.1002/adbi.202400819","DOIUrl":"10.1002/adbi.202400819","url":null,"abstract":"Guilongwan (GLW), a representative of traditional Chinese Medicine (TCM) has been utilized to treating diabetic foot ulcer (DFU)-related syndrome including an intolerance of cold with cold limbs, blood circulation disorder, and immune dysfunction for decades. However, the chemical and biological mechanisms of GLW remain unclear. This study aims to discover the biological mechanisms of GLW on DFU by using streptozotocin- and skin-puncher-induced DFU rat models, in vitro macrophage models, and in silico analysis. The alterations in pathology, Notch1 signaling, and macrophage polarization are detected. The results indicated that GLW promoted wound healing, cutaneous cell proliferation, and angiogenesis in DFU rats by inhibiting delta-like (DLL) 4/Notch1 signaling. In addition, GLW inhibited M1 polarization and promoted M2 polarization in diabetic wounds. Seventeen chemical compounds in GLW-medicated serum are identified. In silico analysis and in vitro experiments demonstrated that GLW-medicated serum and its main compounds inhibited the expression of DLL4 in matrix metalloproteinase-9-induced M1 macrophages. In conclusion, GLW ameliorated experimental DFU rats via the inhibition of DLL4/Notch1 signaling in M1 macrophages. This study provided a new biologic mechanism for GLW in the treatment of DFU.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaling Up Synthetic Cell Production Using Robotics and Machine Learning Toward Therapeutic Applications (Adv. Biology 5/2025) 利用机器人技术和机器学习扩大合成细胞生产的治疗应用（Adv. Biology 5/2025）

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-05-14 DOI: 10.1002/adbi.202570100

Noga Sharf-Pauker, Ido Galil, Omer Kfir, Gal Chen, Rotem Menachem, Jeny Shklover, Avi Schroeder, Shanny Ackerman

引用次数: 0

Artificial Biology – Assemble, Imitate, Adapt (Adv. Biology 5/2025) 人工生物学-组装，模仿，适应（生物5/2025）

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-05-14 DOI: 10.1002/adbi.202570102

Brigitte Städler, Alexander N. Zelikin, Julián Valero, Ebbe Sloth Andersen, Samuel Sánchez

引用次数: 0

Artificial Biology – Assemble, Imitate, Adapt 人工生物学-组装，模仿，适应

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-05-14 DOI: 10.1002/adbi.202500236

Brigitte Städler, Alexander N. Zelikin, Julián Valero, Ebbe Sloth Andersen, Samuel Sánchez

引用次数: 0

Paliperidone Inhibits Ferroptosis Mediated by Autophagy in Renal Tubular Epithelial Cells by Targeting CHAC1 帕利哌酮通过靶向CHAC1抑制肾小管上皮细胞自噬介导的铁下垂。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-04-21 DOI: 10.1002/adbi.202400648

Xiangrong Ying, Ke Gao, Zhengang Luo, Yu Ren, Chong Shen, Haojie Zhang, Chuanchuan Zhan, Zibin Xu, Jintao Wu, Gangfeng Wu

{"title":"Paliperidone Inhibits Ferroptosis Mediated by Autophagy in Renal Tubular Epithelial Cells by Targeting CHAC1","authors":"Xiangrong Ying, Ke Gao, Zhengang Luo, Yu Ren, Chong Shen, Haojie Zhang, Chuanchuan Zhan, Zibin Xu, Jintao Wu, Gangfeng Wu","doi":"10.1002/adbi.202400648","DOIUrl":"10.1002/adbi.202400648","url":null,"abstract":"Renal tubular epithelial cell injury is a significant factor in the formation of kidney stones. However, the regulatory mechanisms behind this injury, especially the association with autophagy-mediated ferroptosis, remain unclear. This study first identified the upregulated ferroptosis related gene ChaC Glutathione Specific Gamma-Glutamylcyclotransferase 1 (CHAC1) in kidney stone samples through bioinformatics analysis. Subsequently, a damage model is established by treating renal tubular epithelial cells (HK-2) cells with calcium oxalate (CaOx) and investigated its function by downregulating CHAC1 expression through shRNA transfection. Autophagy status and oxidative stress are evaluated by detecting autophagy (LC3I, LC3II, Beclin 1) and ferroptosis (GPX4) related protein expression using GFP-LC3 adenovirus and Western Blot. In addition, the interaction between small molecule drug Paliperidone (Pali) and CHAC1 is also investigated through molecular docking and cell thermal migration assays to explore therapeutic potential. CHAC1 is upregulated in kidney stones and associated with ferroptosis. Knockdown of CHAC1 weakened CaOx-induced autophagy and ferroptosis. Moreover, Pali can target CHAC1 protein, reduce CHAC1 activity, and inhibit autophagy-mediated ferroptosis during cellular injury. Pali can inhibit autophagy-mediated ferroptosis in renal tubular epithelial cells by targeting CHAC1, offering a new direction for the treatment of kidney stones.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Biologically Active Glass Fiber Functionalized Using Magnesium Phosphate Cement Promotes Bone and Vascular Regeneration 磷酸镁水泥功能化新型生物活性玻璃纤维促进骨和血管再生。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-04-17 DOI: 10.1002/adbi.202400800

Yuzheng Lu, Yanbo Shan, Yingjie Xiong, Jianting Ye, Yanbin Wu, Jipeng Song, Yao Zhang, Wancheng Lin, Haoye Meng, Wenjing Xu, Jiang Peng, Qiang Lu, Lixiang Ding

{"title":"Novel Biologically Active Glass Fiber Functionalized Using Magnesium Phosphate Cement Promotes Bone and Vascular Regeneration","authors":"Yuzheng Lu, Yanbo Shan, Yingjie Xiong, Jianting Ye, Yanbin Wu, Jipeng Song, Yao Zhang, Wancheng Lin, Haoye Meng, Wenjing Xu, Jiang Peng, Qiang Lu, Lixiang Ding","doi":"10.1002/adbi.202400800","DOIUrl":"10.1002/adbi.202400800","url":null,"abstract":"Magnesium phosphate cement (MPC) continues to gain attention in the field of biomedicine. However, its suboptimal mechanical strength and weak biological activity hinder its wider clinical application. Given the excellent biological characteristics of bioglass fiber (BGF), In this study, magnesium phosphate bone cement (BMPC) containing MPC and BGF with different concentrations (0%, 10%, 20%) are fabricated. Called (MPC, 10BMPC, 20BMPC) respectively. BGF-induced mechanical strengthening is verified through physical and chemical performance tests. In vitro experiments showed that BMPC have better osteogenic properties than MPC and can enhance the proliferation and adhesion capacity of human umbilical vein endothelial cells. In vivo experiment, 20BMPC can significantly promote bone regeneration and vascular network formation, and histological analysis further confirmed the osteogenic capacity of 20BMPC. Transcriptomic analyses confirmed that the activities of the Notch pathway and Hif1 pathway are upregulated in the 20BMPC group, reflecting the strong interconnection between osteogenesis and angiogenesis. 20BMPC, which have the highest BGF content, showed the best performance among all the tested materials. This study showed that BGF improved the mechanical strength of bone cement and enhanced its osteogenic and angiogenic abilities. Therefore, 20BMPC can be used as a new bone repair material.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400800","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucus Physically Restricts Influenza A Viral Particle Access to the Epithelium (Adv. Biology 4/2025) 黏液物理限制甲型流感病毒颗粒进入上皮（Adv. Biology 4/2025）

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-04-16 DOI: 10.1002/adbi.202570097

Logan Kaler, Elizabeth M. Engle, Maria Corkran, Ethan Iverson, Allison Boboltz, Maxinne A. Ignacio, Taj Yeruva, Margaret A. Scull, Gregg A. Duncan

引用次数: 0

3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function (Adv. Biology 4/2025) 3D机械约束指导肌肉干细胞的命运和功能（Adv. Biology 4/2025）

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-04-16 DOI: 10.1002/adbi.202570098

GaYoung Park, Josh A. Grey, Foteini Mourkioti, Woojin M. Han

引用次数: 0

PTEN Regulates Myofibroblast Activation in Valvular Interstitial Cells Based on Subcellular Localization 基于亚细胞定位的PTEN调节瓣膜间质细胞中肌成纤维细胞的激活。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-04-14 DOI: 10.1002/adbi.202400540

Dilara Batan, Georgios Tseropoulos, Bruce E. Kirkpatrick, Carrie Bishop, Kaustav Bera, Alex Khang, Mary Weiser-Evans, Kristi S. Anseth

{"title":"PTEN Regulates Myofibroblast Activation in Valvular Interstitial Cells Based on Subcellular Localization","authors":"Dilara Batan, Georgios Tseropoulos, Bruce E. Kirkpatrick, Carrie Bishop, Kaustav Bera, Alex Khang, Mary Weiser-Evans, Kristi S. Anseth","doi":"10.1002/adbi.202400540","DOIUrl":"10.1002/adbi.202400540","url":null,"abstract":"Aortic valve stenosis (AVS) is characterized by altered mechanics of the valve leaflets, which disrupts blood flow through the aorta and can cause left ventricle hypotrophy. These changes in the valve tissue result in the activation of resident valvular interstitial cells (VICs) into myofibroblasts, which have increased levels of αSMA in their stress fibers. The persistence of VIC myofibroblast activation is a hallmark of AVS. In recent years, the tumor suppressor gene phosphatase and tensin homolog (PTEN) has emerged as an important player in the regulation of fibrosis in various tissues (e.g., lung, skin), which motivated to investigate PTEN as a potential protective factor against matrix-induced myofibroblast activation in VICs. In aortic valve samples from humans, high levels of PTEN are found in healthy tissue and low levels of PTEN in diseased tissue. Then, using pharmacological inducers to treat VIC cultures, it is observed that PTEN overexpression prevented stiffness-induced myofibroblast activation, whereas genetic and pharmacological inhibition of PTEN further activated myofibroblasts. The increased nuclear PTEN localization is also observed in VICs cultured on stiff matrices, and nuclear PTEN also correlated with smaller nuclei, altered expression of histones, and a quiescent fibroblast phenotype. Together, these results suggest that PTEN not only suppresses VIC activation, but functions to promote quiescence, and can serve as a potential pharmacological target for the treatment of AVS.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0