Nanotechnologies in Russia最新文献

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Nondestructive Testing of Polymer Composites with a Honeycomb Filler Based on the Root Mean Square Temperature Deviatiosn Recorded in the Process of Active Thermography 基于主动热成像记录均方根温度偏差的蜂窝填料聚合物复合材料无损检测
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-24 DOI: 10.1134/S263516762460281X
D. Yu. Golovin, A. G. Divin, A. A. Samodurov, Yu. A. Zaharov, A. I. Tyurin, Yu. I. Golovin
{"title":"Nondestructive Testing of Polymer Composites with a Honeycomb Filler Based on the Root Mean Square Temperature Deviatiosn Recorded in the Process of Active Thermography","authors":"D. Yu. Golovin,&nbsp;A. G. Divin,&nbsp;A. A. Samodurov,&nbsp;Yu. A. Zaharov,&nbsp;A. I. Tyurin,&nbsp;Yu. I. Golovin","doi":"10.1134/S263516762460281X","DOIUrl":"10.1134/S263516762460281X","url":null,"abstract":"<p>Polymer composites and composites including nanoscale structural elements in particular are being increasingly applied in various fields of industry. One of the actively developing types of such materials is composites with a honeycomb filler. The specifics of applying these materials requires monitoring and defect detection. However, it is complicated by their anisotropy and significant heterogeneity. The work describes a method of nondestructive testing based on active scanning thermography with a linear heat source, which is suitable for the detection of subsurface defects in nanocomposites with a honeycomb filler. Experiments on model samples with artificially introduced defects are carried out. Mathematical methods and algorithms for defect identification on the basis of thermogram data are developed. Some sources of noise and errors are identified, and measures to reduce their impact are proposed.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 1 supplement","pages":"S175 - S182"},"PeriodicalIF":0.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Socio-Humanitarian Aspects of the Application of Nanobiotechnologies in the Health Care Industry 纳米生物技术在医疗保健行业应用的社会人道主义方面
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S263516762460069X
T. N. Gaeva, T. V. Smirnova, R. G. Vasilov
{"title":"Socio-Humanitarian Aspects of the Application of Nanobiotechnologies in the Health Care Industry","authors":"T. N. Gaeva,&nbsp;T. V. Smirnova,&nbsp;R. G. Vasilov","doi":"10.1134/S263516762460069X","DOIUrl":"10.1134/S263516762460069X","url":null,"abstract":"<p>This review is devoted to analyzing the different aspects of the application of nanobiotechnologies in the health care industry. An assessment of the socio-humanitarian risks that arise in contrast to the invaluable contribution of nanobiotechnologies in improving the health and quality of life of the population is carried out. A principle for the identification and systematization of emerging risks is proposed, taking into account prospects for the development of the transhumanistic strategy of human enhancement using nanotechnologies, which is increasingly popular abroad, together with other convergent technologies. The main factors explaining the lag in the formation of the regulatory framework for applying nanobiotechnologies compared to the pace of scientific and technological development of this area are identified.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"859 - 871"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of C-Phycocyanin on Isolated Mitochondria c -藻蓝蛋白对离体线粒体的影响
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602420
A. P. Kuvirchenkova, T. N. Goleva, D. V. Sukhinov, Ya. E. Sergeeva, R. G. Vasilov
{"title":"Effect of C-Phycocyanin on Isolated Mitochondria","authors":"A. P. Kuvirchenkova,&nbsp;T. N. Goleva,&nbsp;D. V. Sukhinov,&nbsp;Ya. E. Sergeeva,&nbsp;R. G. Vasilov","doi":"10.1134/S2635167624602420","DOIUrl":"10.1134/S2635167624602420","url":null,"abstract":"<p>C-phycocyanin, a water soluble light-harvesting pigment-protein complex of the cyanobacteria photosynthetic apparatus, demonstrates antioxidant, antitumor, and anti-inflammatory effects, and is often used in medical studies. In this work, the effect of C-phycocyanin isolated from the cyanobacterium <i>Arthrospira platensis</i> on the bioenergetic parameters of rat-liver mitochondria is examined. It is shown that C-phycocyanin does not exhibit uncoupling, depolarization or inhibition of the respiratory chain action for isolated rat-liver mitochondria, does not reduce the rate of ATP synthesis, but does exhibit a strong antioxidant effect.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"1023 - 1029"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the Electrochemical Synthesis of Superparamagnetic Iron Oxide Nanoparticles with Low Coercivity Using a Magnetic Field 磁场催化合成低矫顽力超顺磁性氧化铁纳米颗粒的研究
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602250
M. A. Gritsai, A. A. Kuzharov, V. A. Roldugin, V. V. Butova, A. V. Soldatov, M. A. Soldatov
{"title":"On the Electrochemical Synthesis of Superparamagnetic Iron Oxide Nanoparticles with Low Coercivity Using a Magnetic Field","authors":"M. A. Gritsai,&nbsp;A. A. Kuzharov,&nbsp;V. A. Roldugin,&nbsp;V. V. Butova,&nbsp;A. V. Soldatov,&nbsp;M. A. Soldatov","doi":"10.1134/S2635167624602250","DOIUrl":"10.1134/S2635167624602250","url":null,"abstract":"<p>An improved electrochemical method for the synthesis of superparamagnetic iron oxide nanoparticles (NPs) with low coercivity is presented, which provides a high yield and good potential for scalability. The electrochemical synthesis method is optimized by changing parameters such as the composition of the electrolyte solution, the configuration of the electrodes, sonication during the synthesis process, and the use of a magnetic field for the selective sampling of magnetic NPs from the reaction zone. The obtained NPs are studied by X-ray diffraction analysis, transmission electron microscopy, X-ray absorption spectroscopy, and vibrational magnetometry. The X-ray diffraction patterns show that the synthesized magnetic NPs are dominated by the cubic phase, and according to X-ray absorption spectroscopy data, iron is in the oxidation state Fe<sup>3+</sup>. Taken together, these results indicate the formation of the γ-Fe<sub>2</sub>O<sub>3</sub> phase. According to transmission electron microscopy and X-ray peak broadening data, the average particle size is 13 and 8.6 nm, respectively. Vibrational magnetometry measurements indicate a trend towards decreasing coercivity with decreasing particle size. The obtained magnetic NPs, due to their characteristics, can be applied in the field of biomedicine.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"1044 - 1050"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Analysis of Stabilization Methods for Silver Nanoprisms As Promising Nanobiomedicine Agents 纳米银片稳定方法的比较分析
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624601487
A. O. Antonova, O. A. Kolesnikova, P. M. Ivantcova, M. A. Gubaidullina, V. O. Shipunova
{"title":"Comparative Analysis of Stabilization Methods for Silver Nanoprisms As Promising Nanobiomedicine Agents","authors":"A. O. Antonova,&nbsp;O. A. Kolesnikova,&nbsp;P. M. Ivantcova,&nbsp;M. A. Gubaidullina,&nbsp;V. O. Shipunova","doi":"10.1134/S2635167624601487","DOIUrl":"10.1134/S2635167624601487","url":null,"abstract":"<p>Silver nanoprisms are promising agents for both diagnostic and therapeutic applications in photoinduced hyperthermia of tumors within the transparency window of biological tissues. However, one of the primary challenges in utilizing silver nanoparticles in biological systems is their instability in physiological environments. To address this issue, a comprehensive comparative analysis of various stabilizing coatings was conducted to develop an effective stabilization method for nanoprisms intended for biomedical applications. The results demonstrated that among the tested polymers, a sulfhydryl derivative of polyethylene glycol provided the most effective stabilization for the nanoprisms while preserving the localized surface plasmon resonance effect. Furthermore, the synthesized nanoprisms showed significant potential for biovisualization. Specifically, the technique of intravital optical tomography effectively visualized body tissues using fluorescently labeled nanoprisms. This advancement paves the way for the application of these colloid-stable nanoprisms as agents for bioimaging and photothermal therapy.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"921 - 928"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
α-Synuclein Expression Causes Mitochondria Fragmentation and Increased Sensitivity to Oxidative Stress in the Yeast Yarrowia lipolytica α-突触核蛋白的表达会导致线粒体碎裂并增加酵母脂溶性酵母对氧化应激的敏感性
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602377
V. O. Khvastunov, T. N. Goleva, Kh. Kh. Epremyan, A. G. Rogov
{"title":"α-Synuclein Expression Causes Mitochondria Fragmentation and Increased Sensitivity to Oxidative Stress in the Yeast Yarrowia lipolytica","authors":"V. O. Khvastunov,&nbsp;T. N. Goleva,&nbsp;Kh. Kh. Epremyan,&nbsp;A. G. Rogov","doi":"10.1134/S2635167624602377","DOIUrl":"10.1134/S2635167624602377","url":null,"abstract":"<p>Parkinson’s disease is a chronic neurodegenerative disease that is based on mitochondrial dysfunction. One of the factors in the development of Parkinson’s disease is aggregation of the α-synuclein protein, which makes it a strong biomarker of the disease. α-Synuclein itself is a neuronal protein involved in dopamine transport, but its overexpression or mutations negatively affect cellular homeostasis. Since the disease is complex, identifying the role of mitochondrial dysfunction in its pathogenesis is an important task, and many different models are being developed to study the intracellular features of the disease. The use of yeast seems to be a promising strategy for studying pathologies of this kind. The effect of α-synuclein expression in <i>Yarrowia lipolytica</i> yeast on the mitochondrial morphology and sensitivity to oxidative stress induced by a prooxidant is considered. It is shown that α-synuclein expression leads to the fragmentation of yeast mitochondria and reduces the tolerance of yeast cells to induced oxidative stress.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"1016 - 1022"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Silver-Containing Nanodispersions on the Basis of Polymer Derivatives of Aromatic Amino Acids: Synthesis, Morphology, Spectral Characteristics, and Antimicrobial Activity 基于芳香族氨基酸聚合物衍生物的含银纳米分散体:合成、形态、光谱特性和抗菌活性
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602298
S. V. Valueva, N. A. Nesterova, M. E. Vylegzhanina, P. Yu. Morozova, A. S. Ilchenko, E. P. Anan’eva, E. F. Panarin
{"title":"Silver-Containing Nanodispersions on the Basis of Polymer Derivatives of Aromatic Amino Acids: Synthesis, Morphology, Spectral Characteristics, and Antimicrobial Activity","authors":"S. V. Valueva,&nbsp;N. A. Nesterova,&nbsp;M. E. Vylegzhanina,&nbsp;P. Yu. Morozova,&nbsp;A. S. Ilchenko,&nbsp;E. P. Anan’eva,&nbsp;E. F. Panarin","doi":"10.1134/S2635167624602298","DOIUrl":"10.1134/S2635167624602298","url":null,"abstract":"<p>Nanodispersions based on silver nanoparticles (Ag<sup>0</sup>) are synthesized In the presence of polymer complexones (copolymers of <i>N</i>-vinylpyrrolidone (VP) with <i>p</i>-methacryloylaminobenzoic (MABA-Na) and <i>p</i>-methacryloylaminosalicylic acid (MASA-Na)). The spectral and morphological characteristics of the nano-dispersions are investigated using ultraviolet (UV) spectroscopy and atomic force microscopy (AFM); the minimum energy (<i>E</i><sub>g</sub>) of electron transfer from the valence band to the conduction band of the metal is estimated; and the influence of the nature of the chelate unit on the band gap <i>E</i><sub>g</sub>, as well as on the size of spherical nanoparticles is shown. The reduction of silver ions in water in the presence of copolymers VP-MABA-Na and VP-MASA-Na, due to fairly good screening, makes it possible to obtain spherical nanostructures with a diameter not exceeding 200 nm. In the case of Ag<sup>0</sup>/VP-MASA-Na, massive dense oval-shaped aggregates are formed, which consist of small densely packed nanostructures. The silver-containing dispersions Ag<sup>0</sup>/VPрMABA-Na and Ag<sup>0</sup>/VP-MASA-Na tested against <i>E. coli</i>, <i>P. aeruginosa</i>, and <i>S. aureus</i> bacteria show pronounced antimicrobial activity, significantly superior to the activity of the reference drug, Protargol.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"966 - 972"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure of Supramolecular Clusters of Creatine Kinases in the Intermembrane Space of Mitochondria 线粒体膜间隙肌酸激酶超分子簇的结构
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624601785
Yu. M. Chesnokov, S. V. Nesterov, K. S. Plokhikh, R. G. Vasilov
{"title":"Structure of Supramolecular Clusters of Creatine Kinases in the Intermembrane Space of Mitochondria","authors":"Yu. M. Chesnokov,&nbsp;S. V. Nesterov,&nbsp;K. S. Plokhikh,&nbsp;R. G. Vasilov","doi":"10.1134/S2635167624601785","DOIUrl":"10.1134/S2635167624601785","url":null,"abstract":"<p>The structure of rat-heart mitochondria and mitochondrial membrane preparations is studied using cryoelectron tomography. It is found that pseudocrystalline clusters formed by densely packed proteins exist between the inner and outer membranes. Using subtomogram averaging, the structure of these clusters is obtained and it is shown that they are formed by tens or even hundreds of densely packed octamers of mitochondrial creatine kinases (mCK). In intact mitochondria, mCK octamers form a single membrane-parallel layer in which they are tightly coupled to the outer and inner membranes, as well as to each other. On membrane fragments in which there is no compression of the intermembrane space, the supramolecular mCK clusters are organized differently: they form two layers in which there are fewer contacts with the membrane, but more protein–protein contacts. In addition, bilayer clusters generally lack curvature and are larger in size, which is indicative of their increased rigidity and possible role in stabilizing the membrane when it is damaged. The obtained results are discussed in the context of previously known data on the functional role, structure, and location of creatine kinases in mitochondria.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"1008 - 1015"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conductive-Hydrogel-Based Electrodes for Invasive Neural Interfaces 用于侵入性神经界面的导电水凝胶电极
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602535
D. A. Kirillova, N. A. Sharikova, A. O. Romanov, R. G. Vasilov, T. E. Grigoriev, P. M. Gotovtsev
{"title":"Conductive-Hydrogel-Based Electrodes for Invasive Neural Interfaces","authors":"D. A. Kirillova,&nbsp;N. A. Sharikova,&nbsp;A. O. Romanov,&nbsp;R. G. Vasilov,&nbsp;T. E. Grigoriev,&nbsp;P. M. Gotovtsev","doi":"10.1134/S2635167624602535","DOIUrl":"10.1134/S2635167624602535","url":null,"abstract":"<p>Currently, technologies based on the use of electrode systems implanted in the head interacting with the human brain are under active development. Such systems are used both for stimulation of the brain in various diseases and as full-fledged neural interfaces. Electrodes in this case are one of the key elements, since they provide contact with the nervous tissue. Recently, considerable attention has been paid to polymer electrodes and, in particular, hydrogels due to the fact that they have mechanical properties close to body tissues and can carry various functional molecules. At the same time, the electrical conductivity of polymer electrodes is lower than that of metal or carbon materials. In this regard, this work provides an overview of publications in this area and notes the most promising trends. Also, based on the publications reviewed, it is noted that an important task for such electrodes remains their miniaturization. It is concluded that despite their shortcomings, electrodes based on polymer hydrogels are an extremely promising system for creating invasive neural interfaces.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"872 - 878"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanomaterials in Biosensors for Medical Applications 纳米材料在医学生物传感器中的应用
IF 0.8
Nanotechnologies in Russia Pub Date : 2025-03-16 DOI: 10.1134/S2635167624602353
Y. V. Plekhanova, S. E. Tarasov, A. N. Reshetilov
{"title":"Nanomaterials in Biosensors for Medical Applications","authors":"Y. V. Plekhanova,&nbsp;S. E. Tarasov,&nbsp;A. N. Reshetilov","doi":"10.1134/S2635167624602353","DOIUrl":"10.1134/S2635167624602353","url":null,"abstract":"<p>Biosensors have long been an integral part of analytical laboratory equipment. Some developments have already been commercialized and mass produced for field-test analysis. The best-known biosensors at the moment are those for glucose determination, which are both disposable and long lasting. The use of biosensors for the early diagnosis of diseases would bring medicine to a qualitatively new level, allowing for the more accurate detection of a disease and its individual stages, the adjustment of treatment, and the monitoring of various parameters and biochemical indicators of health in the process of both treatment and during sports training. New electrode modifications, the application of new nanomaterials, the development of new analysis schemes, and the miniaturization of signal processing devices significantly expands the analytical capabilities of the developed biosensors. This paper presents new directions in the development of biosensors for the determination of both traditional markers of the human physiological state (glucose, lactate, etc.) and infectious pathologies (viral, cancerous, etc. diseases), wearable and implantable devices, as well as combined devices for simultaneous diagnostics and drug delivery.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"19 6","pages":"879 - 886"},"PeriodicalIF":0.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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