Moscow University Chemistry Bulletin最新文献_第8页

Complex Formation of Silver(I) with 1,2,3-Benzotriazole: Protonization Constants of 1,2,3-Benzotriazole 银(I)与1,2,3-苯并三唑络合物的形成:1,2,3-苯并三唑的质子化常数

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-06-07 DOI: 10.3103/S0027131423030100

A. S. Samadov, A. F. Stepnova, E. F. Faizullozoda, A. N. Kuzmenko, O. N. Plakhotnaya, A. V. Kuzin, K. J. Suyarov, K. Ya. Khuseinov

引用次数: 0

Groupoid of Intermolecular Contacts and its Fuzzy Cayley Graph 分子间接触群拟及其模糊Cayley图

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-06-07 DOI: 10.3103/S0027131423030033

A. M. Banaru, D. A. Banaru, S. M. Aksenov

引用次数: 0

Crucible Atomizer with the Function of Separating Matrix Components for Determination of Atomic Absorption of Elements in Solid Samples 具有分离基体组分功能的坩埚雾化器测定固体样品中元素的原子吸收

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-06-07 DOI: 10.3103/S0027131423030069

V. N. Oreshkin, G. I. Tsysin

引用次数: 0

Nonlinear Effects in Erythrocyte Sedimentation Dynamics in Whole Blood and in Model Systems after Dilution with Solutions of Polymers (Dextrans). Blood as an Active Colloid 聚合物溶液(右旋糖酐)稀释后全血和模型系统中红细胞沉降动力学的非线性效应。血液是一种活性胶体

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-06-07 DOI: 10.3103/S0027131423030045

E. V. Buravleva, V. L. Voeikov, S. E. Kondakov

{"title":"Nonlinear Effects in Erythrocyte Sedimentation Dynamics in Whole Blood and in Model Systems after Dilution with Solutions of Polymers (Dextrans). Blood as an Active Colloid","authors":"E. V. Buravleva, V. L. Voeikov, S. E. Kondakov","doi":"10.3103/S0027131423030045","DOIUrl":"10.3103/S0027131423030045","url":null,"abstract":"The dynamics of sedimentation of whole blood cells and erythrocyte mass during sequential dilution with dextran solutions in vitro has been studied. Nonmonotonic changes in the optical density of the border between erythrocytes deposited both in their own plasma and in model systems were revealed. Fluctuations in the average and instantaneous rates of the process recorded at the boundary of cell subsidence confirm the nonlinear and cooperative nature of erythrocyte sedimentation, which is associated with the formation and separation of phases between cellular, liquid and gaseous components of blood. The structural reorganization of this dynamic system can be modulated in various ways, for example, by adding dextran solutions used in clinical practice in the case of blood loss, for detoxification, treatment and prevention of thrombophlebitis and shock conditions. It has been shown that the macro- and microkinetics of the erythrocyte sedimentation process depends both on the degree of dilution of blood (or model systems) by dextrans and on their molecular weight. The results obtained are consistent with the phase hypothesis of the settling of blood cells as a biocolloid, and can also be used in clinical hemotransfusiology to select an individual dosage of infusion solutions.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 3","pages":"141 - 144"},"PeriodicalIF":0.7,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4305876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Feed Enzyme Preparations for The Destruction of Nonstarch Polysaccharides and Phytates 用于破坏非淀粉多糖和植酸盐的新型饲料酶制剂

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423020037

O. G. Korotkova, A. M. Rozhkova, V. Y. Kislitsin, O. A. Sinitsyna, Yu. A. Denisenko, M. A. Marochkina, I. N. Zorov, I. A. Shashkov, A. D. Satrutdinov, A. P. Sinitsyn

{"title":"New Feed Enzyme Preparations for The Destruction of Nonstarch Polysaccharides and Phytates","authors":"O. G. Korotkova, A. M. Rozhkova, V. Y. Kislitsin, O. A. Sinitsyna, Yu. A. Denisenko, M. A. Marochkina, I. N. Zorov, I. A. Shashkov, A. D. Satrutdinov, A. P. Sinitsyn","doi":"10.3103/S0027131423020037","DOIUrl":"10.3103/S0027131423020037","url":null,"abstract":"New recombinant strains of Penicillium verruculosum with a high level of expression of homologous endo-β-1,4-glucanase II and heterologous phytase A of A. niger, as well as heterologous endo-1,4-β-xylanase E of P. canescens and phytase A of A. niger, are created. This allows us to obtain highly active feed enzyme preparations (EPs) capable of simultaneously significantly reducing the viscosity of nonstarch polysaccharides (NPSs), as well as increasing the bioavailability of phosphorus and minerals of grain-based feed and, thus, increasing the digestibility of nutrients of agricultural animal and poultry feed. Specific EP activities for specific substrates (xylan, β-glucan, and phytate) are studied and the qualitative and quantitative component composition of new EPs is determined. A recipient strain δnia D, into which heterologous endo-1,4-β-xylanase E of P. canescens is successfully cloned, is obtained based on a new enzyme preparation containing homologous endo-β-1,4-glucanase II and heterologous phytase A of A. niger. In this way, a promising producer of three target enzymes is obtained simultaneously.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 2","pages":"63 - 68"},"PeriodicalIF":0.7,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4920498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect of the TacA Knockout Transcription Factor on the cbhI Gene Transcription and Expression in the Filamentous Fungus Strain Penicillium verruculosum TacA敲除转录因子对丝状真菌疣青霉cbhI基因转录和表达的影响

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423010029

A. M. Chulkin, V. Yu. Kislitsin, I. N. Zorov, I. A. Shashkov, A. M. Rozhkova

{"title":"The Effect of the TacA Knockout Transcription Factor on the cbhI Gene Transcription and Expression in the Filamentous Fungus Strain Penicillium verruculosum","authors":"A. M. Chulkin, V. Yu. Kislitsin, I. N. Zorov, I. A. Shashkov, A. M. Rozhkova","doi":"10.3103/S0027131423010029","DOIUrl":"10.3103/S0027131423010029","url":null,"abstract":"The tacA gene encoding the TacA repressor protein is cloned by the method for “walking” in uncloned DNA from the genomic DNA of the fungus Penicillium verruculosum B1-221-151. Knockout of the tacA and niaD genes by the CRISPR/CAS9 method leads to the production of a new recipient strain P. verruculosum ΔniaDΔtacA, characterized by a higher rate of extracellular protein biosynthesis. Analysis of the cbhI gene transcription and expression in the original P. verruculosum B1-221-151 strain and in the P. verruculosum ΔniaDΔtacA strain shows a sharp increase in the level of the cbhI gene transcription as early as 2 h after the start of induction with cellobiose, cellotriose, gentiobiose, and a mixture of di- and trisaccharides compared with transcription of the cbhI gene in the original strain. The specific activity of cellobiohydrolase I, the main enzyme of the cellulolytic complex of the P. verruculosum fungus, tripled after 96 h of fermentation of the ΔtacA strain compared with the initial strain.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 1","pages":"35 - 41"},"PeriodicalIF":0.7,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5248421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan Nanoparticles: The Drug Delivery System to the Anterior Segment of the Eye 壳聚糖纳米颗粒:眼睛前段的药物输送系统

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423020062

E. V. Popova, V. E. Tikhomirova, O. V. Beznos, N. B. Chesnokova, Y. V. Grigoriev, O. A. Kost

{"title":"Chitosan Nanoparticles: The Drug Delivery System to the Anterior Segment of the Eye","authors":"E. V. Popova, V. E. Tikhomirova, O. V. Beznos, N. B. Chesnokova, Y. V. Grigoriev, O. A. Kost","doi":"10.3103/S0027131423020062","DOIUrl":"10.3103/S0027131423020062","url":null,"abstract":"The effectiveness of drug penetration into the inner tissues of the eye under local application is significantly limited by the barrier effect of the cornea and by the washout of the drug with the lacrimal fluid. To increase the bioavailability of a drug, it is proposed to include the drug in the chitosan particles formed by two types of chitosan: 5 kDa chitosan and 72 kDa glycol-chitosan. Chitosan particles with the loaded angiotensin-converting enzyme inhibitor enalaprilat, capable to reduce intraocular pressure, are characterized by dynamic light scattering and scanning electron microscopy. Particles formed by 5 kDa chitosan have an average hydrodynamic diameter from 85 to 125 nm and a positive ζ potential of +21 ± 3 mV, while particles formed by 72 kDa glycol-chitosan have a size from 440 to 480 nm and are characterized by a ζ potential of +10 ± 2 mV. The percentage of enalaprilat inclusion in the chitosan particles is 25% and 40%, respectively. In vivo experiments showed that the inclusion of the drug int the chitosan particles can increase the retention time of enalaprilat in the lacrimal film of rabbits.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 2","pages":"82 - 88"},"PeriodicalIF":0.7,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4924793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Stable Mutant Bacterial Formate Dehydrogenase with Improved Catalytic Properties 高度稳定的细菌甲酸脱氢酶突变体与改进的催化性能

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423010042

A. A. Pometun, A. A. Shirokova, N. P. Galanicheva, L. A. Shaposhnikov, D. L. Atroshenko, E. V. Pometun, V. I. Tishkov, S. S. Savin

{"title":"Highly Stable Mutant Bacterial Formate Dehydrogenase with Improved Catalytic Properties","authors":"A. A. Pometun, A. A. Shirokova, N. P. Galanicheva, L. A. Shaposhnikov, D. L. Atroshenko, E. V. Pometun, V. I. Tishkov, S. S. Savin","doi":"10.3103/S0027131423010042","DOIUrl":"10.3103/S0027131423010042","url":null,"abstract":"NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) from methylotrophic bacterium Pseudomonas sp.101 (PseFDH) has one of the highest thermal stability among all known enzymes of this group. The introduction of a number of amino acid substitutions into PseFDH made it possible to obtain a multipoint mutant PseFDH SM4S enzyme with even higher temperature and chemical stability. Previously, we showed that the introduction of additional single point replacements S131A, or S160A, or E170D into PseFDH SM4S led to further stabilization of the enzyme. In this work, based on the PseFDH SM4S S131A mutant, new mutant FDHs obtained, in which, compared to PseFDH SM4S, we added double S131A/E170D (M2), triple S131A/S160A/E170D (M3) and quadruple S131A/S160A/E170D/S145A (PseFDH SM4A M3) amino acids replacements. The new PseFDH mutants were overexpressed in E. coli cells, purified and characterized. The S131A/E170D and S131A/S160A/E170D changes provided further improving thermal stability. The introduction of the S145A substitution into PseFDH SM4A M3 leads to a significant decrease in (K_{{text{M}}}^{{{text{NA}}{{{text{D}}}^{ + }}}}) and (K_{{text{M}}}^{{{text{HCO}}{{{text{O}}}^{ - }}}}) while maintaining the catalytic constant at the same level. This mutant form can be successfully used in NADH regeneration systems, as well as for the detection of NAD+ and formate in biological systems.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 1","pages":"20 - 27"},"PeriodicalIF":0.7,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4915651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidases d -氨基酸的生理作用和d -氨基酸氧化酶的生物分析潜力

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423010066

V. I. Tishkov, M. D. Shelomov, A. A. Pometun, S. S. Savin, D. L. Atroshenko

{"title":"Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidases","authors":"V. I. Tishkov, M. D. Shelomov, A. A. Pometun, S. S. Savin, D. L. Atroshenko","doi":"10.3103/S0027131423010066","DOIUrl":"10.3103/S0027131423010066","url":null,"abstract":"D-amino acid oxidase (DAAO) plays an important role in the functioning of both prokaryotes and eukaryotes. DAAO is increasingly being used in practice, including for the determination of D-amino acids in complex samples, involving human tissues and fluids. There are generally two types of DAAO in all organisms. The first type is an enzyme highly specific for D-aspartate and has its own name D-aspartate oxidase (DASPO). DAAO of the second type is characterized by a wide spectrum of substrate specificity, with preference for one or another D-amino acid varying from source to source. The activity of DAAO with a large number of substrates greatly complicates the selective determination of a particular D-amino acid. The problem is often solved by choosing an enzyme that, under the conditions of analysis, has low or no activity with other D-amino acids present in the sample. For the convenience of selecting a particular enzyme, we have collected and analyzed literature data on the catalytic parameters of known DAAOs with the most important D-amino acids. In addition, similar data are presented for novel recombinant DAAOs from the methylotrophic yeast Ogataea parapolymorpha DL-1. Analysis of the data shows that, with the D-amino acid series, the new OpaDASPO and OpaDAAO have the highest catalytic parameters.","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"78 1","pages":"1 - 9"},"PeriodicalIF":0.7,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4919169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkyl-Indole Derivatives of Unnatural Tetrapeptides: Synthesis and Evaluation of Biological Activity 非天然四肽烷基吲哚衍生物:合成及生物活性评价

IF 0.7

Moscow University Chemistry Bulletin Pub Date : 2023-04-24 DOI: 10.3103/S0027131423020086

M. S. Stiazhkina, U. A. Budanova, Y. L. Sebyakin

引用次数: 0