依赖于腔肠素的荧光素酶：特性及在分子分析中的应用

IF 0.7 Q4 CHEMISTRY, MULTIDISCIPLINARY

Moscow University Chemistry Bulletin Pub Date : 2024-07-11 DOI:10.3103/S0027131424700184

L. A. Frank, V. V. Krasitskaya

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引用次数: 0

摘要

摘要本综述介绍了海洋动物生物发光系统的结构数据，这些系统使用依赖于腔肠素的荧光素酶作为发光蛋白。研究表明，这些荧光素酶及其具有新的有用特性的遗传变异体由于反应的可用性、稳定性和高量子产率，被成功地用作各种体外和体内分析系统中的报告分子。它们的应用确保了高灵敏度、简单的设计和快速的分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis

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Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis

This review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as light-emitting proteins. It is shown that these luciferases and their genetic variants with new useful properties are successfully used as reporter molecules in a variety of analytical systems in vitro and in vivo due to the availability, stability, and high quantum yield of the reactions. Their application ensures a high level of sensitivity, simple design, and fast analysis.

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来源期刊

Moscow University Chemistry Bulletin CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

1.30

自引率

14.30%

发文量

期刊介绍： Moscow University Chemistry Bulletin is a journal that publishes review articles, original research articles, and short communications on various areas of basic and applied research in chemistry, including medical chemistry and pharmacology.