识别 "热点"，提高 moxSAASoti 荧光蛋白在 37°C 温度下的成熟度

IF 0.7 Q4 CHEMISTRY, MULTIDISCIPLINARY

Moscow University Chemistry Bulletin Pub Date : 2024-07-11 DOI:10.3103/S0027131424700196

N. K. Marynich, A. P. Savitsky

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引用次数: 0

摘要

摘要我们正在寻找氨基酸残基，替换这些氨基酸残基可使moxSAASoti荧光蛋白在37°C下达到最佳成熟度。对于许多其他荧光蛋白来说，这一特性是通过多轮随机诱变偶然得到改善的；然而，我们设法找到了两个明显影响 moxSAASoti 成熟过程的位置（74 和 125），并通过定点诱变和定点饱和诱变在这两个位置上引入替代物验证了这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Identification of “Hot Spots” to Improve Maturation of the moxSAASoti Fluorescent Protein at 37°C

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Identification of “Hot Spots” to Improve Maturation of the moxSAASoti Fluorescent Protein at 37°C

A search for amino acid residues, whose replacement could contribute to the optimal maturation of the moxSAASoti fluorescent protein at 37°C, is carried out. For many other fluorescent proteins, this characteristic was improved by chance through many rounds of random mutagenesis; however, we managed to find two positions, 74 and 125, which clearly affect the process of moxSAASoti maturation, which is verified by introducing substitutions at these positions by site-directed and site-saturation mutagenesis.

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来源期刊

Moscow University Chemistry Bulletin CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

1.30

自引率

14.30%

发文量

期刊介绍： Moscow University Chemistry Bulletin is a journal that publishes review articles, original research articles, and short communications on various areas of basic and applied research in chemistry, including medical chemistry and pharmacology.