Acta Informatica Medica最新文献

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The Visualization and Frequency of Cerebral Cavernous Angioma on Magnetic Resonance. 脑海绵状血管瘤在磁共振中的可视化和频率。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2024.32.28-31
Advija Mujkic, Svjetlana Mujagic, Zlatan Mehmedovic, Nihad Mesanovic, Renata Hodzic
{"title":"The Visualization and Frequency of Cerebral Cavernous Angioma on Magnetic Resonance.","authors":"Advija Mujkic, Svjetlana Mujagic, Zlatan Mehmedovic, Nihad Mesanovic, Renata Hodzic","doi":"10.5455/aim.2024.32.28-31","DOIUrl":"https://doi.org/10.5455/aim.2024.32.28-31","url":null,"abstract":"<p><strong>Background: </strong>Cavernous angiomas (CAs) are abnormal, congenital, vascular malformations, which often grow in size over the course of life. Conservative treatment, microsurgical resection, and stereotactic radiosurgery are the three main options for treatment of CA. Radiological studies play a key role in diagnosis, with magnetic resonance (MR) being the method of choice.</p><p><strong>Objective: </strong>The aim of this study was to establish the prevalence of cavernous angiomas, the size, appearance, that is, the type of CAs and to determine visualization of cavernous angiomas by magnetic resonance.</p><p><strong>Methods: </strong>The study included all patients who underwent an MR of the brain in the period from January 2011 to the end of December 2017 at the Radiology Clinic of Tuzla University Clinical Centre, and in whom MR examination verified one or more CAs.</p><p><strong>Results: </strong>The prevalence of cavernous angioma in the study was 0.57%, and men and women were equally represented. The number of cavernous angiomas per patient was between 1 and 79 ; the average diameter was 11mm, and the most common type at ≥ 3mm was equivalent to Type II, whilst the largest number of cavernous angiomas, regardless of the size and visualization on individual sequences, were equivalent to Type IV. No significant difference was found in sensitivity between spin echo sequence and T2W gradient echo sequence in the group comprised of cavernous angiomas ≥ 3mm, whilst in the group comprised of punctiform cavernomas < 3mm, T2W* was a significantly more sensitive sequence than spin echo, that is, spin echo sequence had significantly lower sensitivity in the detection of punctiform CAs.</p><p><strong>Conclusion: </strong>The prevalence of CAs was in line with the results of other studies. T2W* sequence is significantly more sensitive in comparison with spin echo only in the detection of punctiform CAs, and is important in the detection of multiple familiar CAs.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"32 1","pages":"28-31"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Secreted IL-10 from Mesenchymal Stem Cell on Immune Checkpoint Molecules. 间充质干细胞分泌IL-10对免疫检查点分子的影响。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.172-175
Mutiara Indah Sari, Nelva Karmila Jusuf, Delfitri Munir, Agung Putra, Tatang Bisri, Syafruddin Ilyas, Farhat Farhat, Adi Muradi Muhar
{"title":"The Effect of Secreted IL-10 from Mesenchymal Stem Cell on Immune Checkpoint Molecules.","authors":"Mutiara Indah Sari,&nbsp;Nelva Karmila Jusuf,&nbsp;Delfitri Munir,&nbsp;Agung Putra,&nbsp;Tatang Bisri,&nbsp;Syafruddin Ilyas,&nbsp;Farhat Farhat,&nbsp;Adi Muradi Muhar","doi":"10.5455/aim.2023.31.172-175","DOIUrl":"https://doi.org/10.5455/aim.2023.31.172-175","url":null,"abstract":"<p><strong>Background: </strong>Immunosuppression in sepsis is hypothesized to result from the increased expression of the immune checkpoint molecules programmed death-1 (PD-1) and programmed death ligand-1 (PD-L1). PD-1 and PD-L1 blockade therapies have been reported to increase survival in septic animals. Currently, the interleukin (IL)-10 within mesenchymal stem cell (MSC) secretome is known for its immunomodulatory capacity.</p><p><strong>Objective: </strong>To study the effect of IL-10 within MSC secretome on the expression of immune checkpoints in the rat model of sepsis. <b>Methods:</b> We used 48 male Rattus norvegicus rats in this research and divided them into four groups: sham (rats without sepsis induction and treatment), control (sepsis-induced rats without treatment), T1 (sepsis-induced rats treated with 150 μL of secreted IL-10 from MSC), and T2 (sepsis-induced rats treated with 300 μL of secreted IL-10 from MSC). Forty-eight hours after sepsis induction, we terminated the rats and collected the blood to examine the PD-1 and PD-L1 expression levels.</p><p><strong>Results: </strong>We found a decrease in the relative expression of PD-1 in the septic rat group given 150 μL and 300 μL of secreted IL-10 from MSC compared to the control group, but the decrease was not significant. We also found a decrease in the relative expression of PD-L1 mRNA in the septic rat group given 150 μL and 300 μL of secreted IL-10 from MSC compared to the control group.</p><p><strong>Conclusion: </strong>Administering secreted IL-10 from MSC reduces the expression of PD-1 and PD-L1 in sepsis. These findings suggest that MSC secretome can improve the immunosuppression in sepsis.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"31 3","pages":"172-175"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c4/c8/AIM-31-172.PMC10540748.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41094604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Using Ethanol Extract of Artocarpus Heterophyllus Leaves and Olea Europea Fruit Oil Combination on Facial Skin. 罗汉果叶乙醇提取物与木犀果油联合应用对面部皮肤的影响。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.168-171
Tri Widyawati, Milahayati Daulay
{"title":"Effect of Using Ethanol Extract of Artocarpus Heterophyllus Leaves and Olea Europea Fruit Oil Combination on Facial Skin.","authors":"Tri Widyawati,&nbsp;Milahayati Daulay","doi":"10.5455/aim.2023.31.168-171","DOIUrl":"https://doi.org/10.5455/aim.2023.31.168-171","url":null,"abstract":"<p><strong>Background: </strong><i>Artocarpus heterophyllus (A. heterophyllus)</i> leaves and <i>Olea europea</i> (OE) fruit oil are natural sources that have been traditionally used for health and skin care purpose.</p><p><strong>Objective: </strong>To assess the potential synergistic effect of combining ethanol extract of <i>A. heterophyllus</i> leaves (AHLE) and OE fruit oil in the formulation of clay masks, specifically in terms of their effect on facial skin.</p><p><strong>Methods: </strong>AHLE was obtained by the maceration method, while OE was purchased commercially. Total phenol and flavonoid content were calculated and a DPPH assay was conducted to evaluate the antioxidant properties. Furthermore, the four formulas prepared were F1 (AHLE 5%), F2 (OE 10%), F3 (AHLE 5% + OE 10%), and F4 (AHLE 2.5% + OE 5%). Adult women received weekly facial treatments with the formulated mask for one month. The effect of these treatments was evaluated based on several skin parameters, including moisture, oiliness, texture, collagen levels, pigmentation, sensitivity, and the presence of wrinkles. Furthermore, the data obtained were analyzed using the Wilcoxon sign-ranked test.</p><p><strong>Results: </strong>AHLE contained total phenolic, flavonoid, and antioxidant activity higher than OE. Clay masks in all formulations showed homogeneity and do not contain coarse grain. After four weeks of treatment, the efficacy of the formulations demonstrated a significant effect. F1 exhibited a reduction in wrinkles by 36.27%, while F3 improved oily skin by 21.39%, enhanced skin texture by 44.32%, reduced pigmentation by 30.30%, and decreased skin sensitivity by 49.18%. Furthermore, F4 demonstrated an increase in skin moisture levels by 27.89% and a boost in collagen production by 32.00%.</p><p><strong>Conclusion: </strong>The combination of AHLE and OE at 5% and 10% demonstrated superior effectiveness compared to their individual use.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"31 3","pages":"168-171"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d4/33/AIM-31-168.PMC10540744.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41176890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization of ChatGPT in Medical Education: Applications and Implications for Curriculum Enhancement. ChatGPT 在医学教育中的应用:课程强化的应用和意义。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.300-305
Yasar Ahmed
{"title":"Utilization of ChatGPT in Medical Education: Applications and Implications for Curriculum Enhancement.","authors":"Yasar Ahmed","doi":"10.5455/aim.2023.31.300-305","DOIUrl":"10.5455/aim.2023.31.300-305","url":null,"abstract":"<p><strong>Background: </strong>The integration of artificial intelligence (AI) into medical education has sparked a paradigm shift in pedagogical approaches, reshaping the way medical knowledge is accessed, processed, and applied. Medical education is a dynamic field that demands continuous adaptation to the evolving healthcare landscape. ChatGPT, an advanced AI language model, with its natural language understanding and generation capabilities, offers a multifaceted toolset that enhances various aspects of medical education.</p><p><strong>Objective: </strong>The objective of this paper is to explore how ChatGPT, an advanced AI language model, is transforming medical education by serving as a dynamic information resource and driving curriculum reform. It aims to highlight the multifaceted uses of ChatGPT and its potential to reshape the pedagogical landscape in medical education.</p><p><strong>Methods: </strong>PubMed, Scopus, Web of Science, ERIC, and Google Scholar databases were searched to assess the literature that met the study objectives from 2019 to August 2023 with explicit inclusion and exclusion criteria.</p><p><strong>Results: </strong>The results demonstrate that ChatGPT's applications in medical education are diverse and encompass real-time curriculum adaptation, personalized learning, and collaborative learning. Its capacity to provide immediate and contextually relevant information has the potential to enhance the quality of medical education significantly.</p><p><strong>Conclusion: </strong>ChatGPT's integration into medical education represents a transformative shift in educational approaches. It offers a wide range of capabilities, from serving as a repository of medical knowledge to facilitating collaborative learning. As medical education continues to evolve, ChatGPT emerges as a powerful tool that can reshape pedagogy and drive meaningful curriculum reform to meet the needs of modern healthcare practice.ChatGPT emerges as a transformative tool that holds the potential to reshape the landscape of medical pedagogy and drive meaningful curriculum reform.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"31 4","pages":"300-305"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10875960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technical Factors Influencing the Health Information System in Kosova. 影响科索沃卫生信息系统的技术因素。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.265-269
Ardita Baraku, Naser Ramadani, Roland Bal
{"title":"Technical Factors Influencing the Health Information System in Kosova.","authors":"Ardita Baraku, Naser Ramadani, Roland Bal","doi":"10.5455/aim.2023.31.265-269","DOIUrl":"10.5455/aim.2023.31.265-269","url":null,"abstract":"<p><strong>Background: </strong>Health information systems (HIS) are considered a vital tool to strengthen Low- and Middle-Income Countries' (LMIC) health systems. Unfortunately, little is known about the technical factors of HIS in LMIC.</p><p><strong>Objective: </strong>This study aimed to make an empirical contribution, using the case of Kosova to find out about HIS development, trying to identify dysfunctional areas and opportunities for improvement.</p><p><strong>Methods: </strong>Technical factors of the PRISM framework were analyzed via document analysis, and semi-structured interviews were held with 15 respondents from the Ministry of Health, the National Institute of Public Health of Kosova, and the Hospital Clinical University Services of Kosova. Interviews were transcribed and coded deductively, using the defined theoretical framework to guide the content analysis.</p><p><strong>Results: </strong>The results indicate that MoH considered technical factors while developing HIS in Kosova. Nevertheless, HIS fulfills only some of the essential functions. Barriers to efficient HIS among technical factors are lengthy, time-consuming manual HIS forms, inadequate data from HIS forms for NIPHK analysis, difficulties when changing software and HIS economic operators, and the complexity of HIS functioning.</p><p><strong>Conclusion: </strong>Kosova still faces technical difficulties with an efficient and sustainable HIS system. Complex processes of extensive efforts have yet to produce the desired results, which prevent evidence-based health analysis and informed decision-making in Kosovar healthcare. More research is needed into organizational and behavioral factors influencing HIS efficiency in LMIC.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"31 4","pages":"265-269"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10875932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Wound Healing Using MMP-8 Levels in GCF of Diabetics With Chronic Periodontitis After Diode Laser Assisted Flap Surgery. 二极管激光辅助皮瓣手术后慢性牙周炎糖尿病患者GCF中MMP-8水平对伤口愈合的评估。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.211-215
Kritika Banerjee, Sheela Kumar Gujjari, SubbaRao V Madhunapantula
{"title":"Assessment of Wound Healing Using MMP-8 Levels in GCF of Diabetics With Chronic Periodontitis After Diode Laser Assisted Flap Surgery.","authors":"Kritika Banerjee,&nbsp;Sheela Kumar Gujjari,&nbsp;SubbaRao V Madhunapantula","doi":"10.5455/aim.2023.31.211-215","DOIUrl":"https://doi.org/10.5455/aim.2023.31.211-215","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory cytokines like Matrix Metalloproteinases (MMPs) are associated with the destruction observed in periodontal disease (3). There has been evidence of significant increases in MMP levels in patients with systemic disorders, such as Diabetes Mellitus (DM), which is associated with microvascular complications, causing increased MMP activity, directly or indirectly, due to oxidative stress. Objective: The aim of this study was conducted in order to assess wound healing using MMP-8 levels in GCF of diabetics with chronic periodontitis after diode laser assisted flap surgery.</p><p><strong>Methods: </strong>This interventional, comparative clinical trial, was conducted after obtaining approval from the Institutional Ethics Committee (IEC), (Study protocol number: 48/2020), and registered with Clinical Trials Registry of India (CTRI/2022/07/043898). Purposive sampling technique was used to select 30 patients with chronic periodontitis (15 systemically healthy patients, and 15 diabetic patients), who visited the Department of Periodontology.</p><p><strong>Results: </strong>Out of the 30 patients initially selected, 3 patients were lost to follow up and 1 patient was excluded from the study due to lack of compliance towards oral hygiene maintenance. Intragroup comparison of the clinical parameters at baseline and 3 months in both groups was statistically significant (p=0.000). This concludes that there was statistically significant improvement in the periodontal parameters of non-diabetic patients after diode laser assisted flap surgery. Inter-group comparison of the clinical parameters did not show statistical significance at baseline and at 3 months (p > 0.05). It can be concluded that there was comparable changes in the periodontal parameters in both groups after surgery.</p><p><strong>Conclusion: </strong>MMP-8 could be used as a futuristic tool for assessing wound healing especially in diabetics, so that necessary treatment interventions can be undertaken prior to development of any post-op complications. Laser assisted Modified Widman flap showed noteworthy improvement in the clinical parameters in both groups. Thus, proving that laser assisted MWF surgery is a favorable treatment modality, especially in diabetics who are immunocompromised and prone to infections.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"31 3","pages":"211-215"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/04/AIM-31-211.PMC10540929.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41102576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of Text Patterns Found on Social Networks of Mental Health Reactions to COVID-19. 研究在 COVID-19 心理健康反应社交网络上发现的文本模式。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2024.32.15-18
Denis Cedeno-Moreno, Alan Delgado-Herrera, Nelson Montilla-Herrera, Miguel Vargas-Lombardo
{"title":"Study of Text Patterns Found on Social Networks of Mental Health Reactions to COVID-19.","authors":"Denis Cedeno-Moreno, Alan Delgado-Herrera, Nelson Montilla-Herrera, Miguel Vargas-Lombardo","doi":"10.5455/aim.2024.32.15-18","DOIUrl":"https://doi.org/10.5455/aim.2024.32.15-18","url":null,"abstract":"<p><strong>Background: </strong>SARS-CoV-2 is an infectious disease caused by the coronavirus that was first reported in December 2019 in China and immediately spread around the world causing a pandemic, which has caused countless deaths and cases in global health. Mental health has not gone untouched by this pandemic; due to the lockdown and the vast amounts of information disseminated, the Panamanian population has begun to feel the collateral effects.</p><p><strong>Objective: </strong>We propose classifying tweets using a machine learning (ML) and deep learning (DL) approach and pattern search to make recommendations to the emotional and psychological reactions of the Panamanian population.</p><p><strong>Methods: </strong>Our study has been carried out with a corpus in spanish extracted from X for the automatic classification of texts, from which we have categorized, through the ML&DL approach, the tweets about Covid-19 in Panama, in order to know if the population has suffered any mental health effects.</p><p><strong>Results: </strong>We can say that the ML models provide competitive results in terms of automatic identification of texts with an accuracy of 90%.</p><p><strong>Conclusion: </strong>X is a social network and an important information channel where you can explore, analyze and organize opinions to make better decisions. Text mining and patron search are a natural language processing (NLP) task that, using ML&DL algorithms, can integrate innovative strategies into information and communication technologies.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"32 1","pages":"15-18"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Validity of the Rezum Simulator for Trainees. 针对受训人员的 Rezum 模拟器的有效性。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2024.32.82-87
Ossamah Saleh Alsowayan, Abdullah Mousa Al Zahrani
{"title":"Validity of the Rezum Simulator for Trainees.","authors":"Ossamah Saleh Alsowayan, Abdullah Mousa Al Zahrani","doi":"10.5455/aim.2024.32.82-87","DOIUrl":"https://doi.org/10.5455/aim.2024.32.82-87","url":null,"abstract":"<p><strong>Background: </strong>Simulations have revolutionized surgical training and are an invaluable adjunct tool for augmenting the proficiency of surgeon and patient safety. Before being included in a practical assessment process, simulators need to be using various validity inference frameworks.</p><p><strong>Objective: </strong>We examine the construct validity and reliability of the Rezum simulator.</p><p><strong>Methods: </strong>Seventeen candidates of different professions voluntarily participated in the Rezum simulation workshop. The simulator provides a variety of variable metrics and challenges. Each candidate performed three cases of different difficulty levels with three trials of each case. Validity was measured statistically through a one-way analysis of variance (ANOVA) test, and a p-value of < 0.05 was considered significant. Additional reliability tests were provided, including intraclass correlation coefficients, a Cronbach test (0.7 is considered acceptable), and standard error of measurement.</p><p><strong>Results: </strong>The ANOVA of total scores among candidates was significant (p = 0.029). Senior registrars and consultants had the highest total scores. Procedure times did not differ significantly among candidates (p = 0.169). The reliability test for the total score was 0.899 (0.831-0.942), with a standard error value of 2.75, a standard deviation of 8.67, and a Cronbach alpha value of 0.915.</p><p><strong>Conclusion: </strong>We confer the primer evidence of Rezum simulation as a valid, reliable simulator of most of its metrics.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"32 1","pages":"82-87"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Water Extract Have Superior Cytotoxic Effect Than Ethanolic Extract of Clinacanthus Nutans Leaves in Breast Cancer Stem Cells. 与乙醇提取物相比,水提取物对乳腺癌干细胞的细胞毒性作用更强。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2024.32.4-10
Siti Syarifah, Aldy S Rambe, Agung Putra, M Ichwan, Yunita Sari Pane, Adi Muradi Muhar, Alfi Khatib, Delfitri Munir, M Rusda, Mustafa M Amin
{"title":"Water Extract Have Superior Cytotoxic Effect Than Ethanolic Extract of Clinacanthus Nutans Leaves in Breast Cancer Stem Cells.","authors":"Siti Syarifah, Aldy S Rambe, Agung Putra, M Ichwan, Yunita Sari Pane, Adi Muradi Muhar, Alfi Khatib, Delfitri Munir, M Rusda, Mustafa M Amin","doi":"10.5455/aim.2024.32.4-10","DOIUrl":"https://doi.org/10.5455/aim.2024.32.4-10","url":null,"abstract":"<p><strong>Background: </strong>The rapid development of medical technology in managing breast cancer patients still cannot solve the problem of recurrence and resistance. One of the causes of recurrence and molecular resistance is the presence of breast cancer stem cells (BCSCs). Clinacanthus nutans (C.nutans) is a plant found in Medan, North Sumatra, Indonesia. This plant is believed to have anticancer activity in community.</p><p><strong>Objective: </strong>Our study aimed to assess phytochemical of C.nutans leaves, isolate breast cancer stem cells and determine the cytotoxic effects of the ethanolic extract and water extract of C.nutans leaves on breast cancer stem cells at 24, 48, and 72 h of observation.</p><p><strong>Methods: </strong>We underwent the cytotoxic test by using MTT assay and isolated breast cancer stem cells by using MACS and validated them by mammosphere test.</p><p><strong>Results: </strong>We found alkaloids, flavonoids, glycosides and tannins in simplicia and all extracts. BCSCs was valid with the diameter of the mammosphere BCSCs was > 60 μm. The IC50 values of 100%, 60%, 40%, 20% EE, and WE of C.nutans leaves were 227.30; 46.05; 31.12; 98.54, and 16.16 μg/ml respectively in the first 24 hours. In administering WE of C.nutans leaves, BCSCs viability was decreased at 24,48 and 72 hours of observation, namely 69.29±26%; 75.82 ± 21.02% and 38.94±9.34 % (p < 0.0001).</p><p><strong>Conclusion: </strong>The WE of C.nutans leaves had more substantial cytotoxic potential against BCSCs than the EE. The capability of WE C.nutans leaves to suppress BCSC's viability was time-dependent. The anticancer activity were believed originate from alkaloid and flavonoid group.</p>","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":"32 1","pages":"4-10"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Barriers to the Adoption of Evidence Based Practice in Nursing: a Focus Group Study. 护理领域采用循证实践的障碍:焦点小组研究。
Acta Informatica Medica Pub Date : 2023-01-01 DOI: 10.5455/aim.2023.31.306-311
Maria Pitsillidou, Maria Noula, Zoe Roupa, Antonis Farmakas
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