The Modulatory Effects of Statins, Vitamin E, and Moringa Oleifera Extract on CD3 Expression in Ribociclib-Induced Hepatotoxicity in Rats.

Abstract

Background: The use of Ribociclib leads to hepatotoxicity that may be evaluated by the presence of infiltrated CD3 cells.

Objective: To evaluate the expression level of CD3 in the liver tissue of the control group and other groups exposed to Ribociclib and groups received Ribociclib and other treatments including vitamin E, statin, and and Moringa Olifera.

Methods: This experiment utilized fifty adult male albino rats (9 - 10 weeks old). The rats were randomly assigned into five groups (N = 10). Rats in the Group 1 (control) were given 2 ml normal saline. Group 2 rats received 5 mg/kg of ribociclib, while Group 3 rats received the same dose of ribociclib in conjunction with a daily dose of 200 mg/kg of a statin. Group 4 rats were given ribociclib treatment, alongside a daily dose of Vitamin E. Group 5 rats were given daily doses of 5 mg/kg ribociclib and a 200 mg/kg extract of Moringa Olifera. Following the end of the experiment, all rats were terminated and the liver tissues wee excised and fixed in formalin for 24 hours, processed, and stained for CD3 using indirect immunoperoxidase stain. Liver tissues were examined microscopically and the expression level was evaluated employing Adope photoshop. The relationships between groups were computed by independent T test.

Results: The study showed ribociclib caused hepatotoxicity of liver through the increased expression of CD3 in comparison to the control group. Treatment with statin and vitamin E lowered the expression of CD3, but this was not statistically significant (p>0.05), while the treatment with Moringa Olifera extract lowered significantly the expression of CD3 in the liver (p=0.043).

Conclusion: Giving Statin, Vitamin E, or Moringa Olifera extract can protect the liver from the liver toxicity that ribociclib causes.