Acta Physiologiae Plantarum最新文献

{"title":"Responses of Andrographis paniculata to zinc stress are related to growth, antioxidant enzymes, and andrographolide biosynthesis","authors":"Suraj Kumar Mourya,&nbsp;Praveen Mohil,&nbsp;Jyotsana Bhati,&nbsp;Anil Kumar","doi":"10.1007/s11738-025-03786-5","DOIUrl":"10.1007/s11738-025-03786-5","url":null,"abstract":"<div><p>Zinc (Zn) is an essential nutrient for plants and is required for normal growth and metabolism. Many enzymes involved in primary metabolism like respiration, photosynthesis, and biosynthesis of plant hormones, possess Zn as a cofactor for their moiety. Zn at elevated concentrations in soil has hazardous effects on plants and leads to retarded cell division and elongation, affects growth and biomass production, impairs photosynthesis, and is often linked with oxidative stress by inducing reactive oxygen species (ROS). <i>Andrographis paniculata</i> is one of the popular medicinal herbs in Asia. It serves a pivotal role in Ayurveda as a traditional medicine and a source of anti-cancerous drug andrographolide (AG). The current study focused on exploring the enhancement of AG content within the plant under zinc stress. The study highlights the impact of various concentrations of Zn (50, 100, and 150 mg kg⁻¹) as zinc sulphate (ZnSO₄ × 7H₂O) on plant health, antioxidative enzymes and elicitation of AG biosynthesis in <i>A. paniculata</i> in a pot experiment with sandy loam soil in the greenhouse. Growth attributes such as plant height and fresh and dry biomass of seedlings were reduced with consistent increases in concentrations of Zn. The activities of enzymes such as catalase, guaiacol peroxidase, peroxidase, ascorbate peroxidase, and glutathione reductase rose with increasing concentrations of Zn. The maximum applied concentration (150 mg Zn kg⁻¹) showed the maximum activity of all studied antioxidative enzymes. Elevated zinc concentrations in soil correlate with increased total phenolic content, MDA content, electrolytic leakage, and H₂O₂ levels in <i>A. paniculata</i>. As the Zn content in soil increased, the AG contents in <i>A. paniculata</i> leaves increased and was maximum recorded at 150 mg kg⁻¹ of Zn. The Zn levels in root and shoot demonstrated a marked linear correlation with the concentrations of Zn applied to the soil. Results suggest that <i>A. paniculata</i> confer Zn tolerance by modulating antioxidative enzymes and increased synthesis of AG. The present finding is the first report on Zn-mediated AG production in <i>A. paniculata</i>.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short alternating light/dark periods priming improves tobacco (Nicotiana tabacum L.) tolerance to clomazone herbicide: growth, chlorophyll a fluorescence transient and photosynthesis rate","authors":"Majd Darwish,&nbsp;Chayma Ouhibi,&nbsp;Habib Issa Abboud,&nbsp;Hala Adnan Alajouria,&nbsp;Félicie Lopez-Lauri,&nbsp;Huguette Sallanon","doi":"10.1007/s11738-025-03778-5","DOIUrl":"10.1007/s11738-025-03778-5","url":null,"abstract":"<div><p><i>Nicotiana tabacum</i> L. <i>cv. Virginie vk51</i> seedlings at the three-leaf stage were grown in a hydroponic culture system. Those seedlings were subjected to a photoperiod priming treatment with alternating light/dark periods (AL) (16 min light/8 min dark cycles and a photosynthetic photon flux density (PPFD) of 50 μmol photons m⁻² s⁻¹) for 3 days. After 2 weeks, the plantlets were transferred to two soil types with 0 (control) or 100 µM clomazone. Compared with the non-primed plants, the AL-primed plants exhibited greater clomazone tolerance with respect to all of the growth parameters and during all of the growth stages. This AL priming effect was demonstrated by increases in plant leaf area and dry and fresh plant weights, which might have been related to increases in the CO₂ assimilation rate (P_n and P_max). Furthermore, the reduced photon absorption (ABS/RC) and heat dissipation (DI0/RC) rates as well as the observed increase in photosystem II (PSII) efficiency (the maximum quantum yield of PSII (F_v/F_m), photochemical quenching (<i>q</i>P), actual PSII efficiency (Ф_PSII)) and electron transport (the light-saturated electron transport rate (ETR) and electron flux beyond the first quinone electron acceptor of PSII (Q_A) evaluated as (1–V_J)) provide strong evidence of a higher tolerance to clomazone. Collectively, these results suggest that AL priming treatment could potentially improve the protection or production of other transplanted species.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous application of melatonin and jasmonic acid protects the sugar beet from heat stress by modulating the enzymatic antioxidants deference mechanism and accumulation of organic osmolytes","authors":"Muhammad Irfan,&nbsp;Ahmed Abou El-Yazied,&nbsp;Muhammad Sheeraz,&nbsp;Shabir Hussain,&nbsp;Abdul Sattar,&nbsp;Qasim Ali,&nbsp;Hany G. Abd El-Gawad,&nbsp;Fahad Mohammed Alzuaibr,&nbsp;Maha Mohammed Alharbi,&nbsp;Siham M. Al-Balawi,&nbsp;Doaa Bahaa Eldin Darwish,&nbsp;Eldessoky S. Dessoky,&nbsp;Mohamed F. M. Ibrahim","doi":"10.1007/s11738-025-03784-7","DOIUrl":"10.1007/s11738-025-03784-7","url":null,"abstract":"<div><p>Heat stress reduces the growth and productivity of agronomic crops and considered as main hazard for sustainable farming. Melatonin and jasmonic acid play an essential role in mitigating the adversities of abiotic stresses. Therefore; current experiment was performed to assess the potential of sole and collective application of melatonin and jasmonic acid to mitigate the adversities of high temperature on sugar beet. Experimental treatments consisted of control (Ck), water spray, melatonin (150 µM), jasmonic acid (150 µM) and melatonin (150 µM) + jasmonic acid (150 µM) applied on sugar beet plants grown under two temperature levels; normal condition and heat stress (inside sheet). Results indicated that high temperature remarkably decreased leaf chlorophyll pigments, and relative water contents while increased electrolyte leakage, hydrogen peroxide, thiobarbituric acid reactive substances (TBARS), activities of antioxidant enzymes, and concentration of organic osmolytes in sugar beet leaves. However; separate and collective application of melatonin and jasmonic acid alleviate negative impact of high temperature by improving photosynthetic pigments, gas exchange attributes and concentration of osmolytes in the leaves of sugar beet. Application of melatonin + jasmonic acid enhanced the activities of antioxidants which reduced the harmful impact of ROS. Moreover; foliar application of melatonin + jasmonic acid considerably increased the root yield and yield attributes and sugar recovery in heat stress condition. It was concluded that combined application of melatonin + jasmonic alleviate the adversities of heat stress by enhancing the leaves chlorophyll contents, gas exchange attributes, osmo-protectants, activities of enzymatic antioxidants. Finding of the study revealed that collective applied melatonin + jasmonic acid found to be useful techniques for cultivation of sugar beet at high temperature stress.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Effects of cryptogein gene on growth, phenotype and secondary metabolite accumulation in co-transformed roots and plants of Tylophora indica","authors":"Amrita Basu,&nbsp;Dipasree Roychowdhury,&nbsp;Raj Kumar Joshi,&nbsp;Sumita Jha","doi":"10.1007/s11738-025-03787-4","DOIUrl":"10.1007/s11738-025-03787-4","url":null,"abstract":"","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring dynamics in ear-leaf physiology during maize grain filling: genotype and nitrogen impact on source–sink relations and yield","authors":"Sammy Abo-Hamed,&nbsp;Eman M. Elghareeb,&nbsp;Omar El-Shahaby,&nbsp;Farag Ibraheem","doi":"10.1007/s11738-025-03775-8","DOIUrl":"10.1007/s11738-025-03775-8","url":null,"abstract":"<div><p>During maize grain filling, effective coordination between a high source capacity and a robust sink significantly enhances yield. These source–sink relationships are primarily influenced by genotype and nitrogen availability, and achieving a balance between them has been a challenge in modern maize hybrids. In this study, three maize hybrids (B73 × Mo17, B73 × Sids7, and B73 × NC358), sharing B73 as the female parent, were produced, field-grown, and maintained till maturity under limited and sufficient soil nitrogen. The impact of the developing reproductive sink on growth, yield, and dynamic changes in ear-leaf physiology was monitored at 0, 5, 10, 15, and 20 days after pollination. Under limited and adequate N conditions, B73 × NC358 outperformed B73 × Mo17 and B73 × Sids7 in yield and most tested traits. The enhanced yield in B73 × NC358 was associated with increased sink-strength traits and improved source capacity-related morpho- physiological characteristics. As grain filling progressed, B73 × NC358 consistently demonstrated higher biomass accumulation, leaf nitrogen, stover nitrogen, chlorophyll content, total soluble proteins, and elevated activities of nitrate reductase (NR) and glutamine synthetase (GS) compared to the other hybrids. Nitrogen limitation curtails grain yield, growth, and leaf metabolites; however, it induces starch accumulation and increased protease and asparaginase (ASNase) activities in all hybrids. Our findings suggest that B73 × NC358 optimizes leaf nitrogen and balances source capacity and sink strength to enhance biomass, nitrogen use efficiency (NUE), and grain yield. The alleles from B73 and NC358 interact effectively to support a stay-green-like phenotype, promoting growth and grain yield across nitrogen conditions.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03775-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of the taproot meristem region in contrasting orange carrot breeding lines under varying levels of drought stress","authors":"Usman Khalid Chaudhry,&nbsp;Muhammad Daniyal Junaid,&nbsp;Ali Fuat Gökçe,&nbsp;Zahide Neslihan Öztürk","doi":"10.1007/s11738-025-03781-w","DOIUrl":"10.1007/s11738-025-03781-w","url":null,"abstract":"<div><p>Climate change has intensified the impact of abiotic stresses on global crop production, with drought emerging as a significant threat to agricultural productivity. Carrot, a major vegetable crop, is particularly vulnerable to drought, which severely hampers its growth and yield. To address this challenge and ensure food security in the face of current and future climatic shifts, it is important to screen and adapt carrot varieties for improved drought tolerance. Therefore, current study was conducted to observe the response of two different orange carrot lines (B2566B and NB3999) under two drought stress levels (85% and 70%) under semi-controlled greenhouse conditions. Results showed that carrot line B2566B exhibited reduced leaf numbers and a 50% increase in lipid peroxidation under drought stress conditions. Contrarily, carrot line NB3999 showed minimal reductions in root length and diameter. Higher H₂O₂ content was observed in B2566B, while NB3999 had increased total phenolic content, total antioxidant activity, and antioxidant enzymatic activity, indicating its tolerance to drought stress. Physio-biochemical traits in NB3999 confirmed its resilience to drought stress at both 85% and 70% levels. The root meristematic tissue of both lines was used for transcriptomic analysis. RNA-Seq analysis identified 788 genes upregulated in NB3999 under drought conditions compared to B2566B. qRT-PCR validation confirmed significant upregulation of these genes under 70% and 85% drought stress, with two genes, PER (peroxidase N1-like) and CYT (cytochrome P450), showing downregulation. This study provided insights into the transcriptional and biochemical mechanisms underlying the drought tolerance of NB3999, in contrast to the sensitivity observed in B2566B. The findings may support future breeding programs for developing drought-tolerant carrot varieties.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt tolerance mechanisms in five Asteraceae species: seed germination and seedling growth, cellular damage, enzymatic and non-enzymatic antioxidants","authors":"Mehdi Ghiasy-Oskoee,&nbsp;Majid AghaAlikhani","doi":"10.1007/s11738-025-03785-6","DOIUrl":"10.1007/s11738-025-03785-6","url":null,"abstract":"<div><p>Exploring the saline-adapted species and the mechanisms by which they have evolved in saline conditions would be a feasible way to utilize saline soils. Based on this approach, this study aimed to evaluate the seed germination and seedling responses of the five abundant Asteraceae species to salinity stress and determine the antioxidant and non-antioxidant defense strategies by which these species demonstrated variations in salinity tolerance. Milk thistle (<i>Silybum marianum</i>), blessed thistle (<i>Cnicus benedictus</i>), pot marigold (<i>Calendula officinalis</i>), safflower (<i>Carthamus tinctorius</i>), and cardoon (<i>Cynara cardunculus</i>) were subjected to 0 (control), 50, 100, 150, 200 and 250 Mm NaCl concentrations. <i>Calendula officinalis</i> (CO) showed the highest, <i>Silybum marianum</i> (SM) and <i>Cnicus benedictus</i> (CB) moderate, and <i>Carthamus tinctorius</i> (CT) followed by <i>Cynara cardunculus</i> (CC) the least inhibition of seed germination and seedling growth at all given salinity levels. Each species utilized different antioxidant mechanisms in response to salinity. Peroxidase (POX) was the major antioxidative enzyme in resistance species, CT and CC, while catalase and superoxide dismutase were more pronounced in moderate, SM and CB, and susceptible, CO, species, respectively. Besides, all species accumulate a considerable amount of proline in response to salinity, which was more evident in the 150 and 200 Mm NaCl concentrations. From the results, it can be concluded that CT and CC had superior saline-tolerance capacity compared to other species due to their longer seedling roots, higher POX activity, and proline accumulation associated with reduced cellular damage.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, expression and interactive proteins analysis of BjuB022815 in B. juncea","authors":"Jing Zeng,&nbsp;Li Feng,&nbsp;Shiyan Liu,&nbsp;Yating Liang,&nbsp;Lixia Huang","doi":"10.1007/s11738-024-03760-7","DOIUrl":"10.1007/s11738-024-03760-7","url":null,"abstract":"<div><p><i>Brassica juncea</i> is a vegetative vegetable, and its yield and quality can be significantly affected by flowering time. In this study, BjuB022815 with other 33 <i>RLKs</i> gene were further divided into 5 subfamilies, most of which contained protein kinase C-like (PKc-like), Malectin-like and STkc-IRAK domains. <i>BjuB022815</i>, which was significantly upregulated at the reproductive stage, was chosen for further study. To explore the interactions of these proteins, Y2HGold assays were conducted with BjuB022815 as bait and four proteins as preys. Y2H and BiFC (Bimolecular florescence) assays confirmed that BjuB022815 interacted with BjuA018253 and BjuVA09G13910. qRT-PCR revealed that <i>BjuB022815</i> was significantly upregulated in flower buds and flowers. At the developmental stage, <i>BjuB022815</i> and <i>BjuA018253</i> were significantly upregulated in the bolting stage, while <i>BjuVA09G13910</i> was downregulated in the reproductive stages. Subcellular assays revealed that BjuB022815, BjuA018253 and BjuVA09G13910 were located in the nucleus. Based on these results, it speculated that BjuB022815 and BjuA018253 interact in the nucleus and function in flowering time regulation in <i>B. juncea</i>.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-024-03760-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous ascorbic acid mitigates salt‐induced damage in soybean by modulating photosynthesis, antioxidant defense, and ionic homeostasis","authors":"Gabriela Eugenia Ajila-Celi,&nbsp;Luis Felipe Lata-Tenesaca,&nbsp;Kolima Peña Calzada,&nbsp;Rita de Cassia Alves,&nbsp;Mara Cristina Pessôa da Cruz,&nbsp;José Sidnaldo Pinzetta Junior,&nbsp;Willians César Carrega,&nbsp;Priscila Lupino Gratão","doi":"10.1007/s11738-025-03770-z","DOIUrl":"10.1007/s11738-025-03770-z","url":null,"abstract":"<div><p>Ascorbic acid (AsA) is one of the most abundant antioxidants, and can modulate several functions in plants under abiotic stress conditions. However, little is known about the mechanism of limiting harmful effects of salt stress through different exogenous AsA application methods. This study evaluated the effect of AsA application via a nutrient solution, foliar spray, and a combination there of on the adverse effects of salt stress in soybean plants. Two experiments using a 4 × 2 factorial scheme under a randomized block design included a salt-tolerant (M 8372) and salt-sensitive (M-Soy 8222) soybean cultivar. The plants of both cultivars were subjected to 0 and 100 mmol L⁻¹ of NaCl and 0.85 mmol L⁻¹ and 100 mmol L⁻¹ of AsA via the root and leaves. Salt stress reduced the chlorophyll and carotenoid contents and photosynthetic rate and increased Na⁺ accumulation and lipid peroxidation in both cultivars. However, AsA application via the roots and leaves in M 8372 and leaves in M-Soy 8222 limited the adverse effects of salt stress by increasing the antioxidant activity of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and guaiacol peroxidase (GPOX, EC 1.11.1.7), as well as proline and endogenous AsA levels, thereby reducing Na⁺ accumulation in the tissues. In addition, AsA limited damage to the photosynthetic apparatus, as evidenced by the increased photosynthetic pigment content and photosynthetic rate, promoting plant growth. These results provide a new perspective on AsA delivery methods in soybean development under salt stress.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of quantitative trait loci associated with grain quality traits in rice (Oryza sativa L.)","authors":"Nahid Feizi,&nbsp;Atefeh Sabouri,&nbsp;Amin Abedi,&nbsp;Adel Bakhshipour","doi":"10.1007/s11738-025-03780-x","DOIUrl":"10.1007/s11738-025-03780-x","url":null,"abstract":"<div><p>Rice grain quality is a crucial factor in determining its economic value. Numerous studies have identified quantitative trait loci (QTLs) associated with grain quality. However, to date, no meta-analysis of QTLs for rice cooking and eating quality traits has been reported. Therefore, we conducted a meta-analysis of QTLs to identify consensus QTLs located at the same locus across multiple studies, aiming to detect significant meta-QTLs (MQTLs) that can be targeted for marker-assisted selection (MAS) applications. In this study, 26 MQTLs were identified from 193 initial independent QTLs related to grain quality traits, including amylose content (AC), gel consistency (GC), and gelatinization temperature (GT). The substantial reduction in the confidence interval of the initial QTLs compared to the MQTLs (from 20.91 to 7.21 cM) led to the identification of both annotated and putative candidate genes associated with the traits. The highest number of MQTLs (eight) was found on chromosome 6. The phenotypic variance explained (<i>R</i>²) by the MQTLs ranged from 2 to 51%, with an average of 19%. Out of the 561 candidate genes identified within the MQTLs, 461 had available expression data. Analysis of their expression patterns in various rice tissues showed that 181 genes had an expression value (FPKM) greater than 10 in at least one tissue, making them promising candidate genes. Gene ontology (GO) analysis of these promising genes highlights their significant roles in various biological processes in rice. This is the first study to identify meta-QTLs associated with cooking and eating grain quality in rice.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}