Anchal Singh, Sushmita Singh, Kiran K. Reddy, Anuj K. Singh, Kamal Krishna Pal, Rinku Dey, Eugenia P. Lal
{"title":"Salt stress alleviation in peanut through calcium supplementation","authors":"Anchal Singh, Sushmita Singh, Kiran K. Reddy, Anuj K. Singh, Kamal Krishna Pal, Rinku Dey, Eugenia P. Lal","doi":"10.1007/s11738-025-03793-6","DOIUrl":null,"url":null,"abstract":"<div><p>Peanut, being moderately salt sensitive, requires efficient management strategies to endure salt stress. A pot experiment was envisaged to evaluate the effect of exogenous calcium chloride (CaCl<sub>2</sub>) on the underlying physiological and biochemical mechanisms in peanut during salinity stress. Salinity-induced membrane destabilization significantly increased thiobarbituric acid reactive substances (TBARS) and H<sub>2</sub>O<sub>2</sub> (hydrogen peroxide) accumulation with susceptible cultivars (TG 37A and GJG 31) experiencing greater oxidative stress. There was a significant reduction in leaf stomatal conductance and net photosynthetic rate (P<sub><i>N</i></sub>), concomitant with the decrease in shoot and root potassium (K<sup>+</sup>) and shoot calcium (Ca<sup>2+</sup>) concentrations due to greater accumulation of sodium (Na<sup>+</sup>). The salt stress alleviating potential of calcium was exhibited by a significant increase in shoot K<sup>+</sup>/ Na<sup>+</sup> ratio along with elevated Ca<sup>2+</sup> concentrations, which aided in restricting Na<sup>+</sup> accumulation in roots and shoots of calcium-treated, salt-stressed cultivars. This ionic homeostasis was accompanied with a significant increase in membrane stability index with reduced TBARS and H<sub>2</sub>O<sub>2</sub> accumulation, ROS detoxification through elevated total antioxidant activity and increased osmoregulatory compounds such as proline, total phenols and soluble sugars in calcium-treated salt-stressed plants compared to control. The improved pigment retention, stomatal conductance and ionic balance contributed to a significant increase in P<sub><i>N</i></sub>, which was also reflected in the improved yield attributes of the peanut cultivars. Thus, the foliar application of CaCl<sub>2</sub> offers a promising approach to alleviate the adverse impact of salt stress in peanut, particularly at 4 dSm<sup>−1</sup>, which otherwise imposes severe yield losses in the crop.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 4","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-025-03793-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Peanut, being moderately salt sensitive, requires efficient management strategies to endure salt stress. A pot experiment was envisaged to evaluate the effect of exogenous calcium chloride (CaCl2) on the underlying physiological and biochemical mechanisms in peanut during salinity stress. Salinity-induced membrane destabilization significantly increased thiobarbituric acid reactive substances (TBARS) and H2O2 (hydrogen peroxide) accumulation with susceptible cultivars (TG 37A and GJG 31) experiencing greater oxidative stress. There was a significant reduction in leaf stomatal conductance and net photosynthetic rate (PN), concomitant with the decrease in shoot and root potassium (K+) and shoot calcium (Ca2+) concentrations due to greater accumulation of sodium (Na+). The salt stress alleviating potential of calcium was exhibited by a significant increase in shoot K+/ Na+ ratio along with elevated Ca2+ concentrations, which aided in restricting Na+ accumulation in roots and shoots of calcium-treated, salt-stressed cultivars. This ionic homeostasis was accompanied with a significant increase in membrane stability index with reduced TBARS and H2O2 accumulation, ROS detoxification through elevated total antioxidant activity and increased osmoregulatory compounds such as proline, total phenols and soluble sugars in calcium-treated salt-stressed plants compared to control. The improved pigment retention, stomatal conductance and ionic balance contributed to a significant increase in PN, which was also reflected in the improved yield attributes of the peanut cultivars. Thus, the foliar application of CaCl2 offers a promising approach to alleviate the adverse impact of salt stress in peanut, particularly at 4 dSm−1, which otherwise imposes severe yield losses in the crop.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.