不同干旱胁迫水平下对比橙色胡萝卜育种品系直根分生区的转录组分析

IF 2.4 4区 生物学 Q2 PLANT SCIENCES
Usman Khalid Chaudhry, Muhammad Daniyal Junaid, Ali Fuat Gökçe, Zahide Neslihan Öztürk
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引用次数: 0

摘要

气候变化加剧了非生物胁迫对全球作物生产的影响,干旱正在成为农业生产力的重大威胁。胡萝卜是一种主要的蔬菜作物,特别容易受到干旱的影响,这严重阻碍了它的生长和产量。为了应对这一挑战,并在当前和未来气候变化的情况下确保粮食安全,重要的是筛选和调整胡萝卜品种,以提高抗旱性。因此,本研究在半受控温室条件下,对2个橙胡萝卜品系(B2566B和NB3999)在85%和70%干旱胁迫水平下的响应进行了研究。结果表明,干旱胁迫条件下,胡萝卜品系B2566B叶片数量减少,脂质过氧化水平升高50%。相反,胡萝卜品系NB3999的根长和根径减少幅度最小。B2566B的H2O2含量较高,而NB3999的总酚含量、总抗氧化活性和抗氧化酶活性均有所提高,说明其耐干旱胁迫。生理生化指标表明,NB3999对干旱胁迫的抗逆性达到85%和70%。对两个品系的根分生组织进行转录组学分析。RNA-Seq分析发现,与B2566B相比,NB3999在干旱条件下上调了788个基因。qRT-PCR验证证实,在70%和85%的干旱胁迫下,这些基因显著上调,其中两个基因PER(过氧化物酶n1样)和CYT(细胞色素P450)下调。该研究揭示了NB3999耐旱性的转录和生化机制,与B2566B的敏感性形成对比。这一发现可能会支持未来培育耐旱胡萝卜品种的育种计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptome analysis of the taproot meristem region in contrasting orange carrot breeding lines under varying levels of drought stress

Transcriptome analysis of the taproot meristem region in contrasting orange carrot breeding lines under varying levels of drought stress

Climate change has intensified the impact of abiotic stresses on global crop production, with drought emerging as a significant threat to agricultural productivity. Carrot, a major vegetable crop, is particularly vulnerable to drought, which severely hampers its growth and yield. To address this challenge and ensure food security in the face of current and future climatic shifts, it is important to screen and adapt carrot varieties for improved drought tolerance. Therefore, current study was conducted to observe the response of two different orange carrot lines (B2566B and NB3999) under two drought stress levels (85% and 70%) under semi-controlled greenhouse conditions. Results showed that carrot line B2566B exhibited reduced leaf numbers and a 50% increase in lipid peroxidation under drought stress conditions. Contrarily, carrot line NB3999 showed minimal reductions in root length and diameter. Higher H2O2 content was observed in B2566B, while NB3999 had increased total phenolic content, total antioxidant activity, and antioxidant enzymatic activity, indicating its tolerance to drought stress. Physio-biochemical traits in NB3999 confirmed its resilience to drought stress at both 85% and 70% levels. The root meristematic tissue of both lines was used for transcriptomic analysis. RNA-Seq analysis identified 788 genes upregulated in NB3999 under drought conditions compared to B2566B. qRT-PCR validation confirmed significant upregulation of these genes under 70% and 85% drought stress, with two genes, PER (peroxidase N1-like) and CYT (cytochrome P450), showing downregulation. This study provided insights into the transcriptional and biochemical mechanisms underlying the drought tolerance of NB3999, in contrast to the sensitivity observed in B2566B. The findings may support future breeding programs for developing drought-tolerant carrot varieties.

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来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
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