Acta Physiologiae Plantarum最新文献

{"title":"Unravelling the proteome of Saccharum officinarum cv. BO91 for novel defense-related proteins during C. falcatum infection","authors":"Ranjit Singh Gujjar,&nbsp;Deeksha Joshi,&nbsp;Sanjay Kumar Goswami,&nbsp;Rajeev Kumar,&nbsp;Arjun Singh,&nbsp;Atul Kumar Upadhyay","doi":"10.1007/s11738-025-03766-9","DOIUrl":"10.1007/s11738-025-03766-9","url":null,"abstract":"<div><p>Red rot, caused by <i>Colletotrichum falcatum</i>, is one of the most destructive diseases of sugarcane that affects the production sugarcane to a huge extent in India. In the present study, moderately red-rot resistant <i>Saccharum officinarum</i> cv. BO91 was inoculated with virulent <i>C. falcatum</i> (CF8) at grand growth phase. Stalk samples were taken at different time intervals to analyze the differentially abundant proteins through nanoLCMS/MS-based proteome analysis. The results indicated that the total number of identified peptides were significantly high (4026) in healthy sugarcane stalks, compared to fungal infected stalks. <i>C. falcatum</i> infection caused a drastic reduction in the number of peptides in sugarcane stalks at different time intervals (2565 at 3 h, 2610 at 6 h, and 2111 at 24 h). However, we found 97 exclusively abundant proteins in fungal infected stalks of sugarcane. Plenty of these proteins have already been functionally characterized in different other crop plants for their association with defense responses. The defense-related proteins included 30S ribosomal protein S15, bifunctional purine biosynthesis protein, CaM-binding domain-containing protein, kaurene synthase-3, DUF family protein, Ig-like domain repeat protein, methyl-accepting chemotaxis protein, O-fucosyltransferase family protein, coproporphyrinogen III oxidase, phenylalanine ammonia-lyase, phosphoglycerate kinase, receptor-like serine/threonine-protein kinase, scarecrow/scarecrow-like protein, and porphobilinogen deaminase. Though majority of commercially grown sugarcane cultivars are susceptible to the red rot pathogen, the proteome of BO91 cultivar displayed the differential abundance of a number of proteins involved in the stimulation of defense responses.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does the phosphorylation of MAP65-1 correlate with the effects of Cr(VI) on Arabidopsis thaliana microtubules?","authors":"Ioannis-Dimosthenis S. Adamakis,&nbsp;Eleftherios P. Eleftheriou","doi":"10.1007/s11738-024-03765-2","DOIUrl":"10.1007/s11738-024-03765-2","url":null,"abstract":"<div><h3>Key message</h3><p>MAP65-1 phosphorylation mediates Cr(VI)-induced microtubule disruption in plants.</p><h3>Abstract</h3><p>Chromium (Cr) exists in two prevalent forms in the environment: trivalent Cr(III) and hexavalent Cr(VI). While Cr(III) can benefit plants as a micronutrient, Cr(VI) is highly toxic and disrupts essential physiological, biochemical, and cellular processes in plants. Plant cell microtubules (MTs) are particularly susceptible to Cr(VI) toxicity, with MAP65-1, a key microtubule-associated protein, playing a crucial role in stabilizing MTs by forming cross-bridges between them. The stability function of MAP65-1 is influenced by its phosphorylation status: phosphorylation causes MAP65-1 to detach from MTs during different cell cycle phases. This study investigated how Cr(VI) affects MAP65-1 presence on cortical MTs in <i>Arabidopsis thaliana</i> roots and hypocotyls. Seedlings expressing GFP:AtMAP65-1 and its non-phosphorylatable variant, AtMAP65-1^9A (GFP:AtMAP65-1^9A), were exposed to 100 μM potassium dichromate (K₂Cr₂O₇, Cr(VI)) on ½ MS solid medium for 24 or 48 h. Confocal laser scanning microscopy (CLSM) revealed that Cr(VI) began impacting cortical MTs within 24 h. The signal intensity of GFP indicated a significant reduction in MAP65-1 presence, whereas the GFP:AtMAP65-1^9A signal remained stable and MTs were unaffected. These results suggest that Cr(VI) toxicity to MTs is linked to MAP65-1’s phosphorylation status.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-024-03765-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the xenobiotic effects of 2,4-dichlorophenoxyacetic acid + 2-methyl-4-chlorophenoxyacetic acid and bromoxynil + 2-methyl-4-chlorophenoxyacetic acid herbicides in wheat","authors":"Sommayeh Abbasyan,&nbsp;Seyedali Peyghambari,&nbsp;Mohhamad-Reza Bihamta,&nbsp;Reza Maali-Amiri,&nbsp;Hassan Alizade","doi":"10.1007/s11738-024-03755-4","DOIUrl":"10.1007/s11738-024-03755-4","url":null,"abstract":"<div><p>Herbicides are xenobiotic compounds. To investigate the xenobiotic effects of foliar herbicide consumption and plant response over time, two commercial selective herbicides (bromoxynil + 2,4-D (H₁), 2,4-D + 2-MCPA (H₂), and the control (zero, H₀)) were applied to seven Iranian wheat genotypes at 50% tillering. The biochemical traits were measured at six-time intervals (0–30th days after treatment). Morphological traits were measured on the 30th day after treatment. The results showed that the applied treatments significantly affected all the measured traits (<i>P</i> &lt; 0.001). Therefore, these traits can be considered screening responses to xenobiotic stress caused by herbicides. For the first time, qualitative Bendict’s reagent quantification and estimation of the proximate content of sucrose as well as the 3,5-dinitrosalicylic acid reagent showed significant variation among genotypes. The change in the treated sample compared to H₀ showed that the effects of stress started at least 30 min after treatment. Consequently, the tolerant plants recovered on the 30th day after treatment. Clustering of the 20 measured traits showed that height, specific leaf weight, and chlorophyll <i>a</i> content could be selected as indicator traits for genotype screening. The genotypes were classified into three groups (sensitive, semi-sensitive, and tolerant) based on indicator traits. The maximum H₂O₂ concentration (a biochemical indicator of the occurrence of stress) was 980.5 µg at 30 min after treatment with H₂ and 735.1 µg on 1th day after treatment with H₁. Finally, the genotypes showed greater sensitivity to the combination herbicide from two biochemical groups of the same type (H₂) than did those from different groups (H₁).</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of drought and cucumber mosaic virus infection on the physiological responses and yield of cowpea (Vigna unguiculata L. Walp.)","authors":"I. O. Adebara,&nbsp;A. O. Adediji,&nbsp;S. A. Adejumo","doi":"10.1007/s11738-024-03756-3","DOIUrl":"10.1007/s11738-024-03756-3","url":null,"abstract":"<div><p>As climate change continues to negatively influence cropping seasons and patterns especially in Africa, field crops are constantly exposed to simultaneous environmental and biotic stresses. Constraints, such as drought and virus infections, occurring either as individual or combined stresses, limit cowpea production. In this study, we investigated the responses of two cowpea varieties with contrasting cucumber mosaic virus (CMV) susceptibilities, ‘Ife-Brown’ (highly susceptible) and ‘IT97K-1042-3’ (moderately susceptible), simultaneously exposed to varying drought regimes and CMV infection. The cowpea plants were mechanically inoculated with CMV nine days after planting and exposed to three levels of water stress (zero, six and 12 days of water stress) at 3 weeks after planting. Data on vegetative and yield parameters, biomass, canopy temperature, chlorophyll and proline contents were collected. The effects of CMV infection alone on cowpea growth and yield parameters were greater than those of drought stress alone, while the combination of CMV and water deficit was more deleterious than the individual treatments. Conversely, six days of water stress improved the growth and yield of both cowpea varieties. Between the two varieties, ‘Ife-Brown’ outperformed ‘IT97K-1042-3’ in terms of growth and biomass accumulation. However, the canopy temperature increased with the number of days of water stress although the values were not statistically different from the control plants. Variations in proline accumulation were observed more positively correlated with drought than with CMV infection. A greater proline content was detected in cowpea exposed to severe drought than in plants subjected only to CMV infection. Combined drought and virus stress reduced cowpea yields while ‘Ife-Brown’ was more tolerant than ‘IT97K-1042-3’. Comparing the effects of CMV infection and drought on both cowpea varieties, ‘Ife-Brown’ had greater vegetative parameters, biomass accumulation and yield than ‘IT97K-1042-3’. The effects of drought and virus infection on cowpea plants, within the context of climate change, are discussed.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous naphthaleneacetic acid (NAA) mitigated the alkaline stress changes in the growth and physio-biochemical performance of maize (Zea mays L.) seedlings","authors":"Babar Iqbal,&nbsp;Arshad Jalal,&nbsp;Naveed Ahmad,&nbsp;Mohammad K. Okla,&nbsp;Hamada Abd Elgawad,&nbsp;Mohamed A. El-Tayeb,&nbsp;Abdel-Rhman Z. Gaafar,&nbsp;Guanlin Li,&nbsp;Daolin Du","doi":"10.1007/s11738-024-03759-0","DOIUrl":"10.1007/s11738-024-03759-0","url":null,"abstract":"<div><p>Naphthaleneacetic acid (NAA) is a synthetic plant hormone, considered to promote plant growth under optimal and stressful conditions. However, its role in regulating the response of crop species, including maize, to alkaline stress (AS) remains largely unknown. In this study, we investigated the effects of exogenous NAA application (160 mg/L) on growth, antioxidant potential, nitrogen assimilation, and ion regulation in maize seedlings subjected to different levels of alkaline stress (0, 30, 60, and 90 mM [1:1 ratio of Na₂CO₃ and NaHCO₃]). We observed that increased alkaline stress) levels significantly increased the concentration of Na⁺, Cl⁻, H₂O₂, and malondialdehyde (MDA), but reduced K⁺ and Mg²⁺ ions compared to control (CK) maize seedlings. Furthermore, glycine betaine, proteins, proline, sugar, and antioxidant enzymes (superoxide dismutase, glutathione reductase, peroxidase, dehydroascorbate reductase, and catalase) were significantly increased, while NO₃⁻ and NH₄⁺ metabolizing enzymes (nitrate reductase, glutamine synthetase, and glutamine oxoglutarate aminotransferase) were significantly reduced. AS induced a negative impact on maize seedlings, which was highly dependent on the concentration of exogenous NAA. Where NAA application reduced Na⁺, Cl⁻, H₂O₂, and MDA levels, but increased K⁺, enriched organic solutes, antioxidant enzyme potential, and N metabolism, and ameliorated stress damage under AS conditions. Conclusively, the NAA application mitigated the adverse effects of AS on maize seedlings. Consequently, it might be an effective strategy for improving morpho-physiological performance in alkalinity-prone areas. However, future molecular studies should examine whether NAA can positively alter maize nutrient composition and thus help resolve nutritional problems in developing countries suffering from alkaline soil.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different species of Tillandsia can be biomonitors of carbon and nitrogen emissions: the case of a tropical metropolitan area in Mexico","authors":"Paula E. Tirado-Zamora,&nbsp;Yareni Perroni,&nbsp;Edison A. Díaz−Álvarez","doi":"10.1007/s11738-024-03762-5","DOIUrl":"10.1007/s11738-024-03762-5","url":null,"abstract":"<div><p>Air pollution affects human health and ecosystems all over the world. However, little attention is paid to its monitoring in tropics, mainly due to the high costs of automated monitoring systems. Biomonitoring may be an alternative, particularly for species of <i>Tillandsia</i> genus, although most species are not yet calibrated for this purpose. Therefore, 1) to determine the biomonitoring potential of <i>T. juncea</i> and <i>T. schiedeana</i> and, 2) to compare the sources and magnitudes of atmospheric pollutants at five urban parks and one rural site in a tropical metropolitan area in Mexico, we measured the elemental and isotopic composition of carbon and nitrogen of four <i>Tillandsia</i> species. Carbon content was consistent across sites and species with no differences. The nitrogen content showed a clear trend of increasing from rural to urban areas, with significantly higher values observed in urban environments. The more negative δ¹³C values were recorded in urban parks, while less negative values were recorded in rural areas, reflecting differences in environmental CO₂ sources. Similarly, δ¹⁵N values were more negative in rural sites and shifted toward positive values in urban areas, showing the influence of anthropogenic nitrogen sources. These highlight the different environmental conditions across the rural and urban landscape. The four species can be used as biomonitors of C and N emissions, since their specific variations reflect the source and concentration of these atmospheric pollutants. Furthermore, the tillandsias showed that pollution in the metropolitan area is different depending on the activity at each site.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium agronomic biofortification and genotypic variability in physiological responses of cowpea plants under field conditions","authors":"Elcio Ferreira Santos,&nbsp;Eduardo Comparsi Filho,&nbsp;Luiz Eduardo Morais Fernandes Fontes,&nbsp;Mateus Andrey Pires Silva,&nbsp;Gutierres Nelson Silva,&nbsp;Alexandre Alonso Oliveira,&nbsp;Maurisrael de Moura Rocha,&nbsp;Vinícius Martins Silva,&nbsp;André Rodrigues Reis","doi":"10.1007/s11738-025-03769-6","DOIUrl":"10.1007/s11738-025-03769-6","url":null,"abstract":"<div><p>Despite its importance as a vital nutrient for animals and humans, selenium (Se) deficiency in plants and human diets is a significant concern due to its low soil concentrations. Cowpea (<i>Vigna unguiculata</i>), a resilient crop widely cultivated in developing nations, shows potential for agronomic biofortification with Se. However, its genotypic diversity and ability to improve essential element uptake are often overlooked in breeding programs. This study aimed to evaluate the impact of Se biofortification in 20 cowpea genotypes, specifically examining the physiological responses related to photosynthetic pigments in leaves, nitrogen compounds, and sugar concentration in grains. Results revealed that Se application led to an increased cowpea yield. Additionally, all genotypes exhibited elevated sucrose and total sugar concentrations in grains, along with increased photosynthetic pigment levels in leaves upon Se supplementation. Notably, the application of Se resulted in increased allantoin, allantoic acid, and total ureide concentrations in all genotypes with highest yield, indicating enhanced nitrogen fixation in cowpea plants by Se in this genotype. These findings provide valuable insights into the potential of Se biofortification to improve the nutritional quality of cowpea grains by increasing Se and sugar concentrations, ultimately enhancing crop yields through improved nitrogen metabolism. This information can guide future breeding programs aimed at enhancing cowpea grain quality and nutritional value through biofortification strategies.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of green and chemically synthesized ZnO nanoparticles on Capsicum annuum under drought stress","authors":"Aysin Guzel Deger,&nbsp;Sertan Çevik,&nbsp;Oskay Kahraman,&nbsp;Ersan Turunc,&nbsp;Ayşegül Yakin,&nbsp;Rıza Binzet","doi":"10.1007/s11738-025-03767-8","DOIUrl":"10.1007/s11738-025-03767-8","url":null,"abstract":"<div><p>Nanoparticle applications have recently become a popular method due to increase the tolerance levels of plants to environmental stresses. In this study, changes induced by exogenous application of green synthesized and commercially purchased zinc oxide nanoparticles (ZnO NPs) (100, 500, 1000 mg/L) to drought-stressed <i>Capsicum annuum</i> plants were studied through comprehensive morphologic, anatomic, physiological and biochemical analyzes. While drought decreased relative water content (RWC) and leaf water potential (LWP), it increased proline, thiobarbituric acid reactive substances (TBARS) content and antioxidant enzyme activities. Exogenous ZnO NPs increased RWC and LWP but they decreased TBARS and proline contents in drought-stressed plants. Furthermore, the data obtained in this study revealed that the response of the antioxidant enzyme varies depending on the concentration of the nanoparticles and the type of synthesis. However, green synthesized ZnO NPs applications (100 and 500 mg/L) were found to be much more effective in reducing the adverse effects of drought stress than those synthesized chemically. The results of this study indicated that although the response of <i>Capsicum annuum</i> plants to ZnO NPs applications varied according to the type and concentration under drought conditions, nanoparticle applications did not lead to a consistent stress response.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03767-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmWRKY17 negatively regulates salt tolerance through ABA signaling pathway in maize","authors":"Zhongxian Ma,&nbsp;Yue Jia,&nbsp;Wanchang Huang,&nbsp;Hao Wu,&nbsp;Xiu Fang,&nbsp;Qing Ma,&nbsp;Ronghao Cai","doi":"10.1007/s11738-024-03763-4","DOIUrl":"10.1007/s11738-024-03763-4","url":null,"abstract":"<div><p>Maize (<i>Zea mays</i> L.) is an important cereal crop worldwide, but production is constrained by salt stress, which disrupts plant cell nutrient metabolism, leading to diminished growth and yield. Therefore, the mining of salt-tolerance genes and breeding of new salt-tolerant germplasm is crucial for improvement of maize yield. In this study, <i>ZmWRKY17</i> (<i>Zm00001d004086</i>) overexpression and mutant lines were exposed to 250-mM and 300-mM NaCl, respectively, for 7 days. Exposed to salt stress, the overexpression lines produced significantly more malondialdehyde and hydrogen peroxide than the control line, accompanied by an increased rate of superoxide anion production and relative electrolyte leakage. The mutant line had a higher percentage survival, higher leaf relative water content, and lower cellular damage under extreme salt stress. According to these findings, ZmWRKY17 appears to have a negative regulatory effect on maize salt tolerance. In addition, under salt stress, <i>ZmWRKY17</i> overexpression plants accumulated more abscisic acid (ABA) than the control plants. The root of <i>ZmWRKY17</i> overexpression plants showed reduced sensitivity to ABA. Based on the results of transcriptome analysis, two candidate genes, <i>Zm00001d016105</i> (<i>ZmPYL10</i>) and <i>Zm00001d016294</i> (<i>ZmPYL3</i>), were screened for regulation by <i>ZmWRKY17</i>. The dual-luciferase experiments demonstrated that ZmWRKY17 binds to the promoter regions of <i>ZmPYL10</i> and <i>ZmPYL3</i>, thereby regulating the expression of downstream genes. In conclusion, this study indicates that ZmWRKY17 is involved in regulating the expression of ABA receptor genes <i>ZmPYL10</i> and <i>ZmPYL3</i> to reduce the sensitivity of plants to ABA and, ultimately, negatively regulate plant salt tolerance.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin induces endoreduplication through oxidative DNA damage triggering lateral root formation in onions","authors":"Sukhendu Maity,&nbsp;Rajkumar Guchhait,&nbsp;Kousik Pramanick","doi":"10.1007/s11738-024-03764-3","DOIUrl":"10.1007/s11738-024-03764-3","url":null,"abstract":"<div><p>This study reports the potential ability of melatonin (Mel) to induce endoreduplication, which may have connections with lateral root formation. Mel induces the lateral root formation in onions in a dose-dependent manner with the highest root forming potential at 50 µM Mel (Mel_2). ROS generation in this dose was significantly higher than the control and a low-dose (5 µM) Mel group (Mel_1), where no lateral roots were observed. Co-treatment of ascorbic acid (AsA) with Mel in the Mel_2 + AsA group can effectively scavenge the Mel_2 induced ROS, resulting in a reduced number of lateral roots in this co-treatment group. These results indicate the connections between the ROS level and the lateral root formation. An increase in DNA content was also observed in the Mel_2 group consistent with the level of ROS-induced DNA damage, suggesting the possible link between ROS-induced DNA damage, endoreduplication, and lateral root formation. The results of gene expression analysis also support the said linkage, where melatonin-induced ROS and DNA damage could initiate the endoreduplication cycle in a dose-dependent manner. The IAA (Indole acetic acid) analysis indicates that IAA accumulation, in the zone of differentiation due to auxin bio-synthesis, triggers lateral root formation in this region in corroboration with endoreduplication and ROS.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}