Acta Parasitologica最新文献

{"title":"Combining Killed Vaccine Candidate with Different Adjuvants to Determine Prophylactic Potential against Leishmaniasis","authors":"Kübra Kelleci,&nbsp;Adil Allahverdiyev,&nbsp;Melahat Bağırova,&nbsp;Murat Ihlamur,&nbsp;Emrah Şefik Abamor","doi":"10.1007/s11686-024-00903-1","DOIUrl":"10.1007/s11686-024-00903-1","url":null,"abstract":"<div><p>Visceral Leishmaniasis is a serious public health problem caused by Leishmania species parasites. Approximately 500 thousand people get Visceral Leishmaniasis (VL) every year. An effective and reliable vaccine against the disease has still not been formulated. Choosing the right adjuvant is important to increase immunogenicity in vaccines prepared with total antigens. In this study, we investigate the ideal adjuvant for use in vaccine formulations against VL. For this purpose, Leishmania antigens (FTLA) obtained from <i>L. infantum</i> parasites by the freeze-thaw method and three different adjuvants (alum-saponin and calcium phosphate) were used. The effectiveness of the formulations was investigated in vitro by cell viability analysis and determination of nitric oxide and cytokine production abilities in J774 macrophage cells. According to the study results, it was determined that formulations prepared with calcium phosphate produced 72% more NO and approximately 7.2 times more IL-12 cytokine. The results obtained showed that calcium phosphate salts can be used as ideal adjuvants in vaccine research against leishmaniasis.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1613 - 1620"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Molecular Analyses of Aponurus laguncula Looss, 1907 (Digenea: Lecithasteridae) Parasitic in Atlantic Spadefish Chaetodipterus faber (Broussonet, 1782) (Acanthuriformes: Ephippidae) from Brazilian Coastal Zone","authors":"A. B. Machado,&nbsp;R. O. Simões,&nbsp;A. Maldonado Jr,&nbsp;M. A. J. Santos,&nbsp;J. L. Luque","doi":"10.1007/s11686-024-00878-z","DOIUrl":"10.1007/s11686-024-00878-z","url":null,"abstract":"<div><h3>Objectives</h3><p>An integrative taxonomic description of <i>Aponurus laguncula</i> (Lecithasteridae), a digenean parasitic species of <i>Chaetodipterus faber</i> (Acanthuriformes) from Brazilian Southeast, is provided. Morphological techniques, as whole mounted slides, histology and scanning electron microscopy, and molecular analyses supported that integrative description.</p><h3>Methods</h3><p>Fifteen digenean specimens were stained in hydrochloric carmine and mounted on permanent slides. Two specimens were stained in hematoxylin and eosin following histological routine processing. Four parasites were dehydrated through a graded ethanol series, critical point dried with carbon dioxide and coated with gold to scanning electron microscopy analysis. Sequence of the large ribosomal subunit (28S rDNA) gene was generated and used to construct a phylogeny based on maximum likelihood and Bayesian inference analyses.</p><h3>Results</h3><p>Morphological description and morphometric data obtained in present study were in accordance with previous studies of the species. Use of another morphological techniques, as scanning electron microscopy and histology, corroborated the observed features of whole mounted slides. Also, they provided a better observation of previous reported characteristics and new features reporting, such as an elongated hermaphroditic duct, a smooth tegument and cells that compose the prostatic gland. The molecular sequence obtained in the present study formed a robust clade with available sequences of species of <i>Aponurus</i>.</p><h3>Conclusions</h3><p>The integrative taxonomic approach successfully combined morphological observations, including both previously reported features and new descriptions from histological and electron microscopy analyses, with molecular data to identify these specimens as <i>A. laguncula</i>. Moreover, the detailed characterization of structures, such as the gonads in <i>A. laguncula</i>, that would be challenging to analyze using a single technique, was possible. Further molecular studies with less conserved genetic markers should be conducted to understand phylogenetic relationships between <i>Aponurus</i> species.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1600 - 1612"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative GRA6 and GRA7 for their Utility as Genetic Markers in the Genotyping of Cerebral Toxoplasmosis in Cerebrospinal Fluid","authors":"Nora Harminarti,&nbsp;Ika Puspa Sari,&nbsp;Wayan Tunas Artama,&nbsp;Darma Imran,&nbsp;Agnes Kurniawan","doi":"10.1007/s11686-024-00901-3","DOIUrl":"10.1007/s11686-024-00901-3","url":null,"abstract":"<div><h3>Introduction</h3><p>Cerebral toxoplasmosis is a severe symptom of <i>Toxoplasma gondii</i> (<i>T. gondii</i>) infection that often affects individuals with Human Immunodeficiency Virus (HIV) infection and can be fatal. <i>T. gondii</i> exhibits diverse strains with varied virulence, such as cerebral toxoplasmosis, which is connected with a specific strain. Molecular methods were used to investigate the genotype of the parasite. Some researchers have used genetic markers, such as the dense granule proteins GRA6 and GRA7, in order to identify <i>T. gondii</i> genotype. This study aimed to evaluate the applicability of GRA6 and GRA7 as genetic markers for determining <i>T. gondii</i> strain from cerebrospinal fluid of AIDS patients with toxoplasmic encephalitis.</p><h3>Method</h3><p>160 serum and cerebrospinal fluid (CSF) samples were collected from 2013 to 2022. The serum samples were initially tested using ELISA anti Toxoplasma IgG, and the CSF was subsequently PCR of 5’SAG2 gene for those positive IgG. A total of 69 CSF successfully positive on PCR of 5’SAG2 were included for analysis of GRA6 and GRA7 by performing PCR, sequencing and phylogenetic analysis for determination of <i>T. gondii</i> type.</p><h3>Result</h3><p>The findings of this study indicate that the use of GRA7 is better than GRA6 when using direct clinical samples. Out of the 69 samples analyzed, total of 36 samples (52.17%) were positive for GRA7. The cases can be classified as type I: 86,1% (31/36), type III: 2,7% (1/36) and atypical: 11,1% (4/36).</p><h3>Conclusion</h3><p>Comparison results between GRA6 and GRA7 for genotype determination shows good results on GRA7. GRA7 can be used as a genetic marker to find out the genotype of <i>T. gondii</i> in direct clinical samples where GRA6 cannot be used.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1555 - 1561"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Sero-Molecular Diagnosis of Toxoplasma gondii and Toxocara spp. Infections in the Police Dogs and Their Trainers in Iran","authors":"Ali Asghari,&nbsp;Shirin Jalili,&nbsp;Nader Azadi","doi":"10.1007/s11686-024-00904-0","DOIUrl":"10.1007/s11686-024-00904-0","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Toxoplasma gondii</i> (<i>T. gondii</i>) and <i>Toxocara</i> spp. are two types of parasites that can infect humans and various animals, including dogs. Police dogs and their trainers have a vital role in law enforcement, and their health and well-being are crucial for them to effectively carry out their duties. No study has yet been conducted on the prevalence of <i>T. gondii</i> and <i>Toxocara</i> spp. infections among police dogs and their trainers in Iran. The objective of this study was to determine the sero-molecular prevalence of <i>T. gondii</i> and <i>Toxocara</i> spp. infections in police dogs and their trainers in Tehran, the capital of Iran.</p><h3>Methods</h3><p>In Tehran province, the anti-narcotics police have nearly 200 well-trained police dogs. Each dog is assigned a dedicated trainer and upon completing missions, is housed separately in a designated area. In the present study, a total of 150 samples were gathered. These included 50 blood samples from randomly selected police dogs, 50 fecal samples from the same dogs, and 50 blood samples from their trainers. The Modified Agglutination Test (MAT) was performed to detect <i>T. gondii</i> antibodies in dog blood samples and the ELISA system was utilized to identify anti-<i>Toxoplasma</i> and anti-<i>Toxocara</i> antibodies in the sera of the dog trainers. A specific segment of the SAG2 and ITS genes were amplified via nested-PCR in order to molecularly detect <i>T. gondii</i> in human blood samples and <i>Toxocara</i> spp. in dog fecal samples.</p><h3>Results</h3><p>Regarding serological findings, the prevalence of <i>T. gondii</i> in dog and human blood samples was 4% (2/50) and 10% (5/50), respectively. According to reports, the seroprevalence of <i>Toxocara</i> spp. in human blood samples was 6% (3/50). No statistically significant association was found between the prevalence of the examined parasites and variables (age, sex, and breed) in dogs, as well as the age variable in military personnel. Molecular findings showed that out of the 50 dog fecal samples and 50 human blood samples, there was no presence of <i>Toxocara</i> spp. and <i>T. gondii</i>, respectively.</p><h3>Conclusion</h3><p>Understanding the prevalence of parasitic infections helps public health officials assess the risk to human and animal populations. This information can guide the development of prevention and control measures to reduce the spread of these infections. Overall, the prevalence of parasitic infections, particularly <i>T. gondii</i> and <i>Toxocara</i> spp., in police dogs and their trainers remains uncertain and necessitates further in-depth research.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1724 - 1728"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal Helminthic Infection among the Population in Northern Thailand","authors":"Ampas Wisetmora,&nbsp;Oranard Wattanawong,&nbsp;Adulsak Wijit,&nbsp;Jutikarn Phukowluan,&nbsp;Ampol Nachairan,&nbsp;Prueksarawuth Jaksuay,&nbsp;Sivapong Sungpradit,&nbsp;Nuttapon Ekobol,&nbsp;Thidarut Boonmars,&nbsp;Alisa Boonsuya,&nbsp;Phornphitcha Pechdee,&nbsp;Chutharat Thanchonnang,&nbsp;Nav La,&nbsp;Nathkapach K. Rattanapitoon,&nbsp;Patpicha Arunsan,&nbsp;Schawanya K. Rattanapitoon","doi":"10.1007/s11686-024-00892-1","DOIUrl":"10.1007/s11686-024-00892-1","url":null,"abstract":"<div><h3>Purpose</h3><p>Gastrointestinal (GI) helminthic infections pose substantial public health threat, particularly in northern Thailand, with a heightened concern in Nan province. This study aims to evaluate the prevalence of GI helminthic infections in Nan province and identify associated risk factors in local population.</p><h3>Methods</h3><p>A cross-sectional survey was conducted in 2022. Fecal specimens were collected and examined using Kato-Katz technique and Formalin Ethyl-Acetate Concentration Technique (FECT). Univariate analysis employed the Chi-square test to explore correlations, while logistic regression was defined risk factors associated with parasite infections.</p><h3>Results</h3><p>Among 739 individuals surveyed, 14.34% were revealed GI helminthic infections. Notably, infection rates were higher in males (20.40%) compared to females (8.95%), with a notable prevalence among individuals aged ≥ 60 (17.78%) and high infection rate was demonstrated in Kiew Chan village (21.67%). The most commonly detected helminths were minute intestinal flukes (MIFs) (10.01%) and <i>Capillaria</i> spp. (3.11%). Males were significantly associated with GI helminthic infections, while individuals aged 40–49 and aged 50–59 commonly displayed of parasite infections. Additionally, Kiew Chan village exhibited a significant association with GI helminthic infections, followed by Sop Puen village. Furthermore, fecal specimens of 13 rodent specimens were frequency revealed <i>Strongyloides</i> spp. (92.30%) and mostly exposed <i>Ancylostoma caninum</i> (41.93%) among 93 canines.</p><h3>Conclusion</h3><p>These findings highlight the imperative for immediate public health interventions in Nan province and analogous areas in northern Thailand. Implementing strategies to enhance sanitation infrastructure and promote hygiene education can significantly contribute for reducing the prevalence of GI helminthic infections and improve overall community health.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1648 - 1660"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Toxoplasma gondii Infection in Yunnan Semi-fine Wool Sheep (Ovis aries) and wild Rodents in Yunnan, China","authors":"Zhao Li,&nbsp;Wen-Jie Cheng,&nbsp;Cai-Qin Deng,&nbsp;Meng-Ling Deng,&nbsp;Hai-Bo Peng,&nbsp;Xing-Quan Zhu,&nbsp;Feng-Cai Zou","doi":"10.1007/s11686-024-00875-2","DOIUrl":"10.1007/s11686-024-00875-2","url":null,"abstract":"<div><h3>Background</h3><p><i>Toxoplasma gondii</i>, a globally distributed zoonotic obligate intracellular parasite, infects a wide array of mammals, including humans, sheep, and birds. As a unique sheep breed in southwestern China, Yunnan semi-fine wool sheep occupies an important position in animal husbandry in Zhaotong due to its strong adaptability, high reproductive rate, and excellent wool quality. Lambs infected with <i>T. gondii</i> are prone to neurological symptoms and growth retardation, while <i>T. gondii</i> infection in ewes can cause abortions, stillbirths, and deformities, thus affecting sheep reproduction and sheep product quality. Meanwhile, mutton and dairy products contaminated with <i>T. gondii</i> can become potential sources of human infection, potentially threatening public health and safety.</p><h3>Method</h3><p>To understand the <i>T. gondii</i> infection in semi-fine wool sheep in Zhaotong, Yunnan Province, 586 blood samples were collected and subjected to indirect hemagglutination assay (IHA) for <i>T. gondii</i> antibodies, and the infection-related factors were analyzed through cross-sectional analysis. In the meantime, nested PCR was conducted on a total of 217 samples collected from 31 rodents caught in and around the sheep breeding ground to test the <i>T. gondii</i> B1 gene in rodent tissues.</p><h3>Results</h3><p>A total of 94 sera tested positive for <i>T. gondii</i> antibodies, with a total positive rate of 16.04% (94/586) (95% CI: 14.77–20.89). Cross-sectional statistical analysis on factors related to semi-fine wool sheep infection rate, including sampling season, sex, age, and weight, suggested that age (&lt; 6 months: 23.81%; 6–12 months: 11.74%; &gt; 12 months: 15.83%) was a significant factor explaining the infection rate differences (<i>P =</i> 0.003 &lt; 0.05, χ² = 11.62, df = 2). Thus, age was considered a key risk factor for <i>T. gondii</i> infection in this study (odds ratio, OR <i>=</i> 2.35, 95% CI: 1.42–3.87). Nested PCR analysis on 217 (heart, liver, spleen, lung, kidney, brain, and muscle) tissues from the 31 rodents indicated that 11 tested positive. The total infection rate of rodents in and around the breeding ground was 35.48% (11/31), and 14 samples tested positive, with a positive infection rate of 6.45% (14/217).</p><h3>Conclusion</h3><p>The <i>T. gondii</i> infection rates of semi-fine wool sheep and rodents from their breeding environment in Zhaotong, Yunnan Province, were high, necessitating enhanced prevention, control, and treatment measures to ensure the healthy breeding of semi-fine wool sheep and veterinary public health and safety.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1592 - 1599"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00875-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Evidence of Leishmania Infantum in Domestic Cats and Associated Risk Factors from the Black Sea Region of Türkiye","authors":"Didem Pekmezci,&nbsp;Alparslan Yildirim,&nbsp;Zeynep Nurselin Kot,&nbsp;Gamze Nur Konacoglu,&nbsp;Onder Duzlu,&nbsp;Gamze Yetismis,&nbsp;Sadullah Uslu,&nbsp;Seray Toz,&nbsp;Yusuf Ozbel,&nbsp;Abdullah Inci,&nbsp;Gokmen Zafer Pekmezci","doi":"10.1007/s11686-024-00885-0","DOIUrl":"10.1007/s11686-024-00885-0","url":null,"abstract":"<div><h3>Purpose</h3><p>The objectives of the present study are to determine the molecular prevalence of <i>Leishmania</i> spp. in the owned domestic cats in the Black Sea Region of Türkiye and analyze the associated risk factors in FeL.</p><h3>Methods</h3><p>Conjunctival swabs (CS), blood, demographic, and clinical data were collected from 150 owned cats brought to the Veterinary Teaching Hospital during 2020–2022. <i>Leishmania</i> kinetoplast DNA (kDNA) from CS was screened by TaqMan Real-Time PCR (qPCR) with the genus-specific primers and a probe.</p><h3>Results</h3><p>All qPCR positive products were also amplified and sequenced to identify <i>Leishmania</i> species by ITS1 primers. Molecular prevalence of <i>L. infantum</i> found as 12.6% (19/150) in the observed cats in the Black Sea Region of Türkiye. There was a significant difference (<i>p</i> &lt; 0.05) between neutered and intact cats with regarding to <i>L. infantum</i> positivity. Intact cats found to be 0.368 times more prone to be <i>L. infantum</i>-positive (L<i>+</i>). Dermatological lesions were found the most common (26.3%) problems in the L <i>+</i> cats. The median leucocyte count was the only parameter that was found statistically (<i>p</i> &lt; 0.05) lower in the <i>L +</i> group (6.60) than the negative group (L−) (8.96), when comparing the WBC, NEU/LYM, MONO/LYM, EOS/LYM and PLT/LYM values.</p><h3>Conclusion</h3><p>This study presented the molecular occurrence of FeL in the Black Sea Region of Türkiye for the first time indicating that the carrier status of the cats makes them alternative reservoirs for possible zoonotic transmission of <i>L. infantum</i> in this zone.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1547 - 1554"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00885-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Biological Enigma: Influence of Host Length and Infection Site on Parasite Abundance in Ompok bimaculatus","authors":"Jit Marick,&nbsp;Subha Shankar Mukherjee,&nbsp;Bhairab Kumar Patra,&nbsp;Anirban Ash","doi":"10.1007/s11686-024-00879-y","DOIUrl":"10.1007/s11686-024-00879-y","url":null,"abstract":"<div><h3>Purpose</h3><p>The influence of two key factors, host length and infection site, on the host-parasite interaction in <i>Ompok bimaculatus</i> (Butter catfish) from Mukutmanipur Dam Lake, were investigated.</p><h3>Methods</h3><p>Present study involved 192 specimens of <i>Ompok bimaculatus</i> with varying body lengths, subjected to diverse statistical analyses. One-way analysis of variance (ANOVA) was performed for the parasite numbers for three groups (cestode, nematode and trematode). Subsequently, we conducted one-way permutational multivariate analysis of variance (PERMANOVA) followed by pairwise test to assess parasite numbers across three body sites (intestine, mesentery, and bodycavity), employing the Bray-Curtis index. Additionally, Principal Coordinate Analysis (PCoA) for the same dataset was performed using the same index. Linear regression analysis was performed for the fish length-cestode number, fish length-nematode number, fish length-trematode number and fish length-total parasite number.</p><h3>Results</h3><p>One-way ANOVA revealed no significant differences in parasite numbers among the three endo-helminth groups (cestode, nematode, and trematode). The results of PERMANOVA revealed significant differences in parasite numbers across the three body sites of the host fishes (groups) (F = 9.41, <i>p</i> = 0.0001). Pairwise tests further demonstrated significant differences between the intestine-mesentery, intestine-body-cavity, and mesentery-body-cavity. Additionally, Principal Coordinate Analysis (PCoA) unveiled a significant relationship between infection site and parasite number. However, linear regression analysis examining the relationship between fish length and parasite abundance indicated no significant associations.</p><h3>Conclusions</h3><p>Through a detailed exploration of the statistical analyses, we provide insights into the host-parasite interaction, elucidating both established knowledge and novel findings in fish parasitology.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1492 - 1500"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00879-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose-6-phosphate Dehydrogenase Variants: Analysing in Indian Plasmodium vivax Patients","authors":"Jahnvi Jakhan,&nbsp;Loick Pradel Kojom Foko,&nbsp;Geetika Narang,&nbsp;Vineeta Singh","doi":"10.1007/s11686-024-00883-2","DOIUrl":"10.1007/s11686-024-00883-2","url":null,"abstract":"<div><h3>Purpose</h3><p>Primaquine (PQ) is recommended for radical cure of <i>Plasmodium vivax</i> (<i>Pv</i>) malaria, but its utilization is still limited due to high risk of severe haemolytic anaemia in patients with glucose-6-phosphate dehydrogenase deficiency (G6PD-d). The aim of the present study is to assess the different genotypic variants leading to G6PD-d in Delhi and Goa regions of India.</p><h3>Methods</h3><p>A total of 46 samples (34 retrospective <i>Pv</i>-mono-infected samples and 12 <i>Pv</i>-uninfected samples) were included in the study. Various genetic variants leading to G6PD-d were analysed by PCR amplification and DNA sequencing of different targeted exons of <i>G6PD</i> gene.</p><h3>Results</h3><p>Molecular analysis showed presence of four mutations in study population viz. 1311 C &gt; T, 34.1% &amp; IVSXI 93T &gt; C, 45.5% and two novel mutations 1388G &gt; T, 2.3% and 1398 C &gt; T, 2.3% (silent mutation). The bioinformatics and computational analysis demonstrate that the slight conformational changes caused by R643L mutation in protein are deleterious in nature.</p><h3>Conclusion</h3><p>The observed mutations do not clarify the role or association between G6PD-d and <i>Pv</i>-infected cases. Further investigation is required in order to fully comprehend and analyse the precise role of these mutations with context to malaria infections.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1522 - 1529"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00883-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Phylogenetic Network Analysis of Nosema apis and Nosema ceranae Isolates from Honeybee Colonies in Türkiye","authors":"Rahşan Koç Akpınar,&nbsp;Ali Tümay Gürler,&nbsp;Cenk Soner Bölükbaş,&nbsp;Selma Kaya,&nbsp;Serhat Arslan,&nbsp;Coşkun Aydın,&nbsp;Şakir Önder Türlek,&nbsp;Sema Nur Çelik,&nbsp;Ayşen Beyazıt,&nbsp;Taraneh Öncel,&nbsp;Ufuk Erol,&nbsp;Ayşe Türkan Çiftci,&nbsp;Zekai Bastem,&nbsp;Hasan Hüseyin Ünal,&nbsp;Mesut Şenel,&nbsp;Arif Bozdeveci,&nbsp;Şengül Alpay Karaoğlu,&nbsp;Murat Yaldız,&nbsp;Gökhan Güven,&nbsp;Bilal Küçükoğlu,&nbsp;Mitat Kurt","doi":"10.1007/s11686-024-00887-y","DOIUrl":"10.1007/s11686-024-00887-y","url":null,"abstract":"<div><h3>Purpose</h3><p>Nosemosis is a disease that infects both Western honeybees (<i>Apis mellifera</i> L.) and Asian honeybees (<i>Apis cerana</i>) and causes colony losses and low productivity worldwide. In order to control nosemosis, it is important to determine the distribution and prevalence of this disease agent in a particular region. For this purpose, a national study was conducted to assess the prevalence of <i>Nosema ceranae</i> and <i>N. apis</i> throughout Türkiye, to perform network analyses of the parasites, and to determine the presence of nosemosis.</p><h3>Methods</h3><p>In this study which aimed to assess the prevalence of <i>N. apis</i> and <i>N. ceranae</i> in different colony types and regions where beekeeping is intensive in Türkiye, specimens were collected from hives with no clinical signs.</p><h3>Results</h3><p>A total of 1194 Western honeybee colonies in 400 apiaries from 40 provinces of Türkiye were examined by microscopic and molecular techniques. Nosemosis was found in all of 40 provinces. The mean prevalence ratio was 64.3 ± 3.0, with 95% CI in apiaries and 40.5 ± 2.9, 95% CI in hives. <i>Nosema ceranae</i> DNA was detected in all of positive hives, while <i>N. ceranae</i> and <i>N. apis</i> co-infection was detected in only four colonies.</p><h3>Conclusion</h3><p>This study showed that nosemosis has spread to all provinces, and it is common in every region of Türkiye. All of the <i>N. ceranae</i> or <i>N. apis</i> samples examined were 100% identical within themselves. Network analysis showed that they were within largest haplotype reported worldwide.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1538 - 1546"},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00887-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}