Acta Parasitologica最新文献

{"title":"Investigation of In Vitro and In Vivo Anti-leishmanial Activity of Auranofin on Leishmania Infantum","authors":"Hadi Sadeghi,&nbsp;Mahdi Delavari,&nbsp;Mohsen Arbabi,&nbsp;Maryam Akhavan Taheri,&nbsp;Sima Rasti,&nbsp;Hossein Hooshyar","doi":"10.1007/s11686-025-01129-5","DOIUrl":"10.1007/s11686-025-01129-5","url":null,"abstract":"<div><h3>Purpose</h3><p>Visceral leishmaniasis (VL) is a serious health-threatening disease. Existing drugs are often toxic. So alternative treatments are constantly being considered and evaluated. This study aims to investigate Auranifin as potential new antileishmanial agent.</p><h3>Methods</h3><p>The fatality rate of different concentrations of auranofin (2, 4, 6, 8, 10, 12 μg/ml) on promastigote and amastigote forms after 24 h was calculated. The induction apoptosis in promastigotes by auranofin was also analyzed. Then, <i>Leishmania</i>-infected mice were treated with a concentration of 6.25 mg/kg and 12.5 mg/kg of Auranofin. After 1 month, the liver and spleen of the euthanized mice were separated, smears were prepared and pathology slides were prepared from the spleen and liver. Blood cell counts and liver and kidney enzymes were analyzed using heart blood. Histopathological examinations were performed on liver and spleen after treatment.</p><h3>Results</h3><p>The fatality rate of auranofin at a concentration of 12 µg/ml on promastigote and amastigote forms of <i>Leishmania infantum</i> 24 h after culture was 99.35% and 94.05%, respectively. Over 90% of promastigotes and amastigotes were killed at concentrations of 8 µg/ml and 12 µg/ml of auranofin, respectively. Flow cytometry showed a high apoptosis rate (93.73%) at a concentration of 12 μg/ml. In the control group (without auranofin) 99% of cells were alive. Histopathological examination of the spleen and liver tissues showed a decrease in the degree of granulomatous and inflammatory changes in the groups treated with auranofin. Blood factors as well as Liver and kidney enzymes showed changes in both the test and control groups.</p><h3>Conclusion</h3><p>Auranofin has anti-leishmanial properties in both promastigotes and amastigotes, further studies are neecessary to explore this aspect.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferric Reductase is a Key Factor in Regulating Iron Absorption by Blastocystis sp.","authors":"Yan Zhao,&nbsp;Chao Zhang,&nbsp;Jing Zhang,&nbsp;Hui-Zhu Nan,&nbsp;Yue Xie,&nbsp;Lei Ma","doi":"10.1007/s11686-025-01127-7","DOIUrl":"10.1007/s11686-025-01127-7","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aimed to identify and analyze the role of Ferric reductase in<i>Blastocystis</i> sp. subtype 2 (ST2) and explore the relationship between the parasite and iron metabolism.</p><h3>Methods</h3><p>The location of <i>Ferric reductase in Blastocystis</i> sp. was determined using the indirect immunofluorescence assay (IFA). Transmission electron microscopy was employed to reveal the effect of iron ions on the cell membrane of<i> Blastocystis</i> sp.. For the first time, RNA interference technology was used to explore the relationship between<i> Ferric reductase</i> and iron ions.</p><h3>Results</h3><p><i>Ferric reductase</i> was distributed in the membrane and cytoplasm of the parasite. Iron reduces the thickness of the<i> Blastocystis</i> sp.'s cell membrane. After silencing the Ferric reductase gene, there was no significant difference in the morphology of the parasite strain compared with the control group. The expression level of the<i> Ferric reductase</i> gene does not play a decisive role in maintaining the morphology of the parasite, but the deletion of the<i> Ferric reductase</i> gene reduces the ability of the parasite to absorb iron ions.</p><h3>Conclusions</h3><p>This study fills the gap in the research field of iron metabolism in<i>Blastocystis</i> sp. among parasites, lays a foundation for the research on the gene function of<i> Blastocystis</i> sp., and provides new candidate factors for the development of<i> Blastocystis</i> sp. vaccines.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145021769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human African Trypanosomiasis (HAT): Epidemiology, Biological Diagnosis and Treatment: A Review","authors":"Patindoilba Marcel Sawadogo,&nbsp;Jean Axel T. Kabore,&nbsp;Kiswendsida Thierry Guiguemde,&nbsp;Issiaka Soulama,&nbsp;Adama Zida","doi":"10.1007/s11686-025-01128-6","DOIUrl":"10.1007/s11686-025-01128-6","url":null,"abstract":"<div><h3>Introduction</h3><p>The objective of the World Health Organization is to achieve the interruption of human African trypanosomiasis (HAT) transmission by 2030.</p><h3>Methods</h3><p>This review aims to update knowledge on HAT, through a synthesis on the epidemiology, diagnostic tools and drugs of HAT.</p><h3>Results</h3><p>From 1960 to 2024 approximately 132,063 cases of HAT have been reported across Africa. The majority of HAT patients live in the Democratic Republic of Congo (DRC). The Card Agglutination Test for Trypanosomiasis (CATT) remained for a long time the reference serology test for field screening. The immune trypanolysis test (ITL) test is an accurate serodiagnostic tool increasingly used for medical surveillance of sleeping sickness, but it is reserved for reference laboratories. Prototypes of TDRs such as SD BIOLINE HAT and, HAT Sero-K-SeT have been developed to respond to constraints posed with CATT and ITL, but lack specificity. Parasitological diagnosis techniques such as the mini-Anion Exchange by Centrifugation technique (mAECT) are used for mandatory confirmation of the disease before the initiation of treatment, but their sensitivity is low. To date, the active molecules against HAT are: pentamidine, suramin, melarsoprol, eflornithine and nifurtimox. The use of these molecules does not guarantee healing and generates many side effects. A new molecule has appeared in the therapeutic arsenal. This is fexinidazole, which was approved by the WHO in 2019 for the treatment of HAT due to <i>T.b. gambiense</i>. The WHO recommends the oral administration of this molecule in the first stage of the disease and in the second stage for non-severe cases. Since 2024, this molecule has also been approved by the WHO for the treatment of HAT due to <i>T. b. rhodesiense</i>. </p><h3>Conclusion</h3><p>All these difficulties raised raise questions about the need to develop new diagnostic tools that are more specific, more sensitive and better suited to field screening. They also call out the urgency of finding new drugs that are less toxic, easy to administer and more effective.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145021773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Behavior of Trypanosoma cruzi in Culture Against Different Dosage Forms of Benznidazole: Experimental Insights","authors":"Jaime López-Domínguez,&nbsp;Aracely López-Monteon,&nbsp;Paulina Ochoa-Martínez,&nbsp;Josue Rafael González-García,&nbsp;Rodolfo Peña-Rodríguez,&nbsp;Etienne Waleckx,&nbsp;Joel Moo-Millán,&nbsp;Angel Ramos-Ligonio","doi":"10.1007/s11686-025-01125-9","DOIUrl":"10.1007/s11686-025-01125-9","url":null,"abstract":"<div><h3>Introduction</h3><p>This study aimed to investigate the influence of benznidazole (BZN) origin on its in vitro potency against various <i>Trypanosoma cruzi</i> strains.</p><h3>Methods</h3><p>Pure BZN, purified BZN, and the pharmaceutical formulation Abarax were evaluated for their activity against several parasite strains.</p><h3>Results</h3><p>Results demonstrated significant variability in BZN’s effectiveness, contingent upon both its specific form and the <i>T. cruzi</i> strain under investigation. Half-maximal inhibitory concentrations (IC₅₀ values) exhibited a wide range, indicating that the potency of BZN is not uniform across all its forms or against all parasite strains.</p><h3>Conclusion</h3><p>In conclusion, this study elucidates the complex interaction between BZN and <i>T. cruzi</i>. The parasite’s response to BZN can vary even within a single strain, influenced by the drug’s specific presentation. These findings underscore the critical importance of considering both parasite diversity and drug formulation when assessing the efficacy of treatments in laboratory assays.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associative Genetic Diversity of Theileria Annulata Calf Isolates from Northern India Based on Cyt B Gene","authors":"Sanjeev Kumar,&nbsp;Daya Shanker,&nbsp;Vikrant Sudan,&nbsp;Shanker Kumar Singh","doi":"10.1007/s11686-025-01126-8","DOIUrl":"10.1007/s11686-025-01126-8","url":null,"abstract":"<div><h3>Purpose</h3><p>Molecular epidemiological studies focusing on the phylogenetic characterization of <i>Theileria annulata</i> are crucial for understanding the evolutionary history of the parasite worldwide. The current study reveals genetic diversity among Indian isolates of <i>T. annulata</i> based on the <i>cyt b</i> gene.</p><h3>Method</h3><p>In the present study, <i>cyt b</i> gene from 6 calf isolates of <i>T. annulata</i> was amplified; custom sequenced and accession numbers: MH778940-MH778945 were obtained.</p><h3>Result</h3><p>Two haplotypes were identified, differing by nucleotide substitutions at positions 710 (thymine to adenine) and 1076 (cytosine to guanine). Isolates from Northern India formed a distinct cluster on the phylogenetic tree compared to those from Southern India and showed closer phylogenetic similarity to Iranian isolates than to other Asian counterparts.</p><h3>Conclusion</h3><p>Important phylogenetic data has been generated from the present study suggestive of marked genetic variability in <i>T. annulata</i> isolates across the globe.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Genetic Variability and Population Structure of Aedes albopictus Populations in Southeast Brazil","authors":"Angela Maria Palacio-Cortés,&nbsp;Bryan Steven Valencia-Marin,&nbsp;Mario Antônio Navarro-Silva","doi":"10.1007/s11686-025-01115-x","DOIUrl":"10.1007/s11686-025-01115-x","url":null,"abstract":"<div><h3>Objective</h3><p>Aedes (Stegomyia) albopictus, commonly known as the Asian Tiger mosquito, is native to tropical and subtropical regions of South and East Asia and has rapidly spread globally. Due to its role as a vector of several medically important arboviruses, understanding its genetic diversity and dispersal patterns is crucial for effective disease control. This study aimed to evaluate the genetic structure insights of Ae. albopictus populations in southeastern Brazil.</p><h3>Methods</h3><p>Mosquito samples from five populations in southeastern Brazil were analyzed using two mitochondrial genes: cytochrome c oxidase subunit 1 (COI) and NADH dehydrogenase subunit 5 (ND5). Phylogenetic analysis and population genetic metrics were employed to assess patterns of genetic diversity and population structure.</p><h3>Results</h3><p>Moderate haplotype diversity was observed, with five COI haplotypes (Hd = 0.43) and eleven ND5 haplotypes (Hd = 0.52), along with low nucleotide diversity. Neutrality tests for ND5 yielded significantly negative values (Fs = -2.435*). Phylogenetic trees identified two major clades, with ND5 haplotypes from Paranaguá and Guaraqueçaba clustering together. A positive correlation between genetic and geographic distance (COI r = 0.78; ND5 r = 0.69) proposing isolation by distance.</p><h3>Conclusion</h3><p>The close genetic relationships and limited variation among Brazilian Ae. albopictus populations indicate ongoing gene flow and a shared ancestry. The association of Paraná haplotypes with Asian lineages suggests a genetic link to the region of origin of species, although the timeline of introduction remains uncertain. These results provide important molecular insights to support vector surveillance and control efforts in southern Brazil.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144909726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Risk Factor Analysis of Bovine Trypanosoma Infection in Cattle across Three Egyptian Governorates","authors":"Abdelfattah Selim,&nbsp;Mohamed Marzok,&nbsp;Hattan S. Gattan,&nbsp;Mohammed H. Alruhaili,&nbsp;Hesham Ismail,&nbsp;Abdelrahman M. Hereba","doi":"10.1007/s11686-025-01118-8","DOIUrl":"10.1007/s11686-025-01118-8","url":null,"abstract":"<div><p>A cross-sectional study was conducted in three governorates of the Nile Delta region in Egypt from January to December 2024. The objective was to determine the seroprevalence of bovine trypanosomosis and evaluate associated risk factors. A total of 540 cattle blood samples were examined using the CATT/<i>T. evansi</i> test, and relevant animal data were analyzed to identify risk associations. The overall prevalence of trypanosomosis was 24.4% (132/540), with the highest rate observed in Kafr El-Sheikh governorate at 26.1% (49/188). The prevalence was significantly associated with age, packed cell volume (PCV), and body condition score (<i>P</i> &lt; 0.05). Multivariable logistic regression analysis showed that the likelihood of infection increased fourfold in cattle older than 3 years, twofold in anemic animals, and threefold in those with poor body condition. The findings indicate that bovine trypanosomosis is a prevalent among examined cattle in the area studied. Therefore, the implementation of strategic prevention and control programs is essential to improve livestock health and productivity.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic Mites of Honey Bees (Apis Spp.): A Detailed Review of Varroa destructor in Parasitism, Pathogen Transmission and its Management","authors":"Ganesan Jeyapriya,&nbsp;Ettiappan Sumathi,&nbsp;Vangili Ramasamy Saminathan,&nbsp;Perumal Renukadevi,&nbsp;Ramasamy Sasikala,&nbsp;Sundaravadivel Sathiya Priya,&nbsp;Sivakumar Kowsika,&nbsp;Subramanian Pradeep","doi":"10.1007/s11686-025-01124-w","DOIUrl":"10.1007/s11686-025-01124-w","url":null,"abstract":"<div><p>The parasitic mite <i>Varroa destructor</i> is the primary factor contributing to global honeybee (<i>Apis mellifera</i>) colony losses, posing a sustainable challenge to apiculture and pollination services. Its intricate life cycle adaptive reproductive strategies, and advanced sensory mechanisms have facilitated its emergence as the most destructive honeybee parasite. <i>V. destructor</i> uses highly specialized feeding strategies that extract essential nutrients from their hosts and introduce various pathogens, causing honeybee health problems. The mite functions as a viral vector, particularly in the case of the transmission and proliferation of deformed wing virus (DWV), which has resulted in significant colony weakening and collapse. <i>V. destructor</i> has emerged as the most destructive ectoparasite of honeybees, compromising both individual bee health and overall colony resilience. Its success is attributed not only to its direct feeding behavior and viral vectoring ability, but also to advanced chemical communication, immune suppression, and behavioral adaptations. Synergistic mite-pathogen interactions highlight the need for effective control measures. Current control approaches include advanced detection systems in the form of Var-Gor, focused neural and viral pathway inhibitions, and other control measures such as essential oils. The development of synergistic management strategies involving biotechnology, genetic resistance, and sustainable treatment alternatives, is critical to control <i>V. destructor</i> infestations. A deeper understanding of the evolutionary arms race between honeybees and <i>V. destructors</i> will be crucial to the development of long-term, sustainable control strategies that safeguard bee populations and preserve pollination services, which are vital to world agriculture. This review aims to synthesize current understanding of <i>V.</i> <i>destructor</i> biology, its interactions with honeybee host (<i>A. mellifera</i> and <i>A. cerana</i>), and the associated microbial and viral pathogens. We also explore recent developments in detection, population dynamics, and sustainable management strategies including botanicals, essential oils, and organic acids. By integrating ecological, physiological, and molecular perspectives, this review highlights the need for multidisciplinary approaches to effectively manage <i>Varroa</i> and mitigate its impact on global apiculture.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Expression Levels of miR-155, miR-133a, and miR-146b in the Serum of Acute and Chronic Forms of Cutaneous Leishmaniasis","authors":"Mohammad Hamedanipour,&nbsp;Zohre Khosravany,&nbsp;Zahra Mirabedini,&nbsp;Mehdi Mohebali,&nbsp;Mohammad Barati,&nbsp;Hamed Mirjalali","doi":"10.1007/s11686-025-01123-x","DOIUrl":"10.1007/s11686-025-01123-x","url":null,"abstract":"<div><h3>Background</h3><p>Leishmaniasis is a vector-borne infectious disease caused by intracellular protozoan parasites of the genus <i>Leishmania</i>. MicroRNAs (miRNAs) can influence the progress and outcome of the disease. This study aimed to investigate the expression levels of miR-155, miR-133a, and miR-146b in the serum of acute and chronic forms of cutaneous leishmaniasis (CL) in Iran.</p><h3>Methods</h3><p>Samples were collected from 30 clinical cases of CL in Golestan province, who suffered from either acute or chronic forms of the disease, including 15 for each. <i>Leishmania</i> species were identified using PCR (kDNA gene). Total RNA was extracted from serum samples, complementary DNA (cDNA) was synthesized based on the loop technique, and the expression levels of miRNAs (miR-155, miR-133a, and miR-146b) were determined through quantitative real-time PCR analysis.</p><h3>Results</h3><p>Conventional PCR on kDNA confirmed the presence of <i>L. major</i> in 30 patients. Healing after one course of treatment and no response to treatment were considered as acute and chronic forms, respectively. Although there were no statistically significant changes, the expression of miR-133a and miR-155 was upregulated in patients with acute CL compared to those with chronic form, while miR-146b was downregulated in patients with acute CL.</p><h3>Conclusions</h3><p>In the current study, the expression changes of miR-155, miR-146b, and miR-133a in acute patients was compared to those patients with chronic CL. Although it was not significant, alterations in the expression levels of miRNAs were observed between acute and chronic forms of CL suggesting different pathogenesis of clinical forms.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Record of Gyrodactylus perccotti Ergens & Yukhimenko, 1973 (Monopisthocotyla: Gyrodactylidae), A Parasite of the Alien Chinese Sleeper (Perccottus glenii Dybowski, 1877), in Bulgaria","authors":"Nina Vancheva,&nbsp;Boyko B. Georgiev","doi":"10.1007/s11686-025-01120-0","DOIUrl":"10.1007/s11686-025-01120-0","url":null,"abstract":"<div><h3>Purpose</h3><p>The Chinese sleeper <i>Perccottus glenii</i> is native in East Asia and has become invasive in West Siberia and Europe. It was recorded in the Bulgarian section of the Danube in 2005. The aim of the present study is to report on the presence of gyrodactylid infection in this alien fish species.</p><h3>Methods</h3><p>In the spring of 2019, two specimens of <i>P. glenii</i> were caught from the Lake Srebarna Biosphere Reserve (Northeastern Bulgaria) and examined for ectoparasites. The gyrodactylid specimens found were studied morphologically in glycerine-gelatine mounts. The ITS1-5.8 S-ITS2 fragment of rDNA was analysed.</p><h3>Results</h3><p><i>Gyrodactylus perccotti</i>, a specific parasite of the Chinese sleeper, was found on the caudal fin of one of the studied specimens with low abundance, two specimens only. The morphology and the DNA analysis showed full correspondence with the previously published data.</p><h3>Conclusions</h3><p>This finding represents the first record of <i>G. perccotti</i> from Bulgaria. The results of the present study increase the knowledge of the non-native geographical range of <i>G. perccotti</i>.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}