Acta Parasitologica最新文献

{"title":"Genetic and Population Diversity of Temnocephalids Associated with the Invasive Crayfish Cherax quadricarinatus in Malaysia","authors":"Norhan N. Azri-Shah,&nbsp;Nor Asma Husna Yusoff,&nbsp;Farizan Abdullah,&nbsp;Ahmad Najmi Ishak,&nbsp;Norainy Mohd Husin,&nbsp;Hin Boo Wee,&nbsp;John Brian Jones,&nbsp;Muhammad Ikhwanuddin,&nbsp;Siti Azizah Mohd Nor,&nbsp;Marina Hassan","doi":"10.1007/s11686-025-01050-x","DOIUrl":"10.1007/s11686-025-01050-x","url":null,"abstract":"<div><h3>Purpose</h3><p>A previous study revealed that temnocephalids <i>Diceratocephala boschmai</i>, <i>Craspedella pedum</i>, and <i>Decadidymus</i> sp. were highly prevalent in <i>Cheraq quadricarinatus</i> in Melaka and Johor, Malaysia.</p><h3>Methods</h3><p>Herein, study was conducted to extract temnocephalids from <i>C. quadricarinatus</i> and identified using a molecular approach via two DNA markers: 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I (CO1). Thereafter, the population diversity of <i>D. boschmai</i> in Malaysia using the CO1 DNA marker was analysed to visualize the genetic associations among populations.</p><h3>Results</h3><p>The analysis of 16 variable (segregating) sites in the CO1 gene alignment of sequence from <i>D. boschmai</i> revealed a calculated haplotype diversity of 0.1266 among populations which indicates genetic variation within the studied groups. Additionally, the determination of nucleotide diversity (II) yielded a value of 0.0035, and Tajima's D neutrality test yielded a negative value for all populations, suggesting no significant departure from neutral evolution.</p><h3>Conclusion</h3><p>AMOVA analysis indicated that the genetic variation or polymorphism is primarily contributed within populations (95%) compared to between populations (4%).</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bomolochidae Claus, 1875 (Cyclopoida: Copepoda) Parasitizing the Marine Fishes of Kerala Coast (India): Host-Parasite Interaction and Species Diversity","authors":"P. Swaraj,&nbsp;M. V. Nikhila Reshmi,&nbsp;K. Rijin,&nbsp;O. K. Drisya,&nbsp;T. A. Jose Priya,&nbsp;Sudha Kappalli","doi":"10.1007/s11686-025-01052-9","DOIUrl":"10.1007/s11686-025-01052-9","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aims to assess region-wise host-parasite interaction and diversity indices of bomolochid species infecting marine fishes along the Kerala coast. Also, it aims to generate COI barcodes and analyse the phylogenetics.</p><h3>Methods</h3><p>Marine fish species along the Kerala coast were sampled for the period 2016–2023 and examined for parasitization by bomolochid copepods. Host preference, prevalence, site-specificity, mean intensity, and biodiversity indices of recovered parasite species were assessed specifically in northern and southern regions of this coast. Mitochondrial COI sequences from bomolochid species were also generated, and their phylogenetic relationships were assessed with those of their counterparts found in the NCBI database.</p><h3>Results</h3><p>Among the sampled fishes, 21 species from 20 genera and 14 families showed parasitization with 20 species of bomolochids. Four host families: Sciaenidae, Acanthuridae, Engraulidae, and Sillaginidae were recorded as new to the bomolochid infection along the Indian coast. Recovered bomolochid species, including nine <i>Bomolochus</i> species, six <i>Nothobomolochus</i> species, two <i>Pumiliopes</i> species, and one each of <i>Pseudorbitacolax</i>, <i>Ceratocolax,</i> and <i>Orbitacolax</i> species, with overall prevalence ranging from 0.28% to 57%. 65% of species prefer single-host, and 35% rely on two or three host species. The northern region encompassed all 20 bomolochid species, whereas only 10 species represented the southern region. Six species showed no region-specific host preference, but their prevalence showed marked differences (4.8–73.8%). The host preference of some bomolochid species spans multiple fish species within the same genus or family, as well as across different genera and families, with a varying degree of prevalence, either region-dependent or independent. The northern region displayed the highest species richness and diversity index compared to the southern region. However, the evenness values from both regions were found to be similar. The mitochondrial COI sequences from five bomolochid species signify the reliability of COI DNA barcoding for the identification of bomolochids at the species level. The analysis of phylogenetic relationships with those of its counterparts found in the NCBI database revealed that Bomolochidae constituted a biphyly topology that is distinct from that of the outgroup, i.e., Harpacticoida.</p><h3>Conclusion</h3><p>The generated information on bomolochid new host record, host preference, prevalence, site specificity, seasonality, diversity and genetic divergence forms the basis not only for further exploration of the species diversity and genetic variation of bomolochids but for analyzing the ecological and evolutionary aspects of species diversity and genetic variation of bomolochids and new host record along the Indian coasts and Indo-pacific region as well. </p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Gastrointestinal Parasites and Associated Risk Factors Among Dairy Cattle in Bangladesh: An 8-Year Retrospective Study","authors":"Delower Hossain,&nbsp;Banya Banowary,&nbsp;Basant Saud,&nbsp;Iqramul Haq,&nbsp;Sabiha Zarin Tasnim Bristi,&nbsp;Ridwan Olamilekan Adesola,&nbsp;Shamsaldeen Ibrahim Saeed,&nbsp;Tanjeem Jannat,&nbsp;Amina Khatun,&nbsp;Umme Kulsum,&nbsp;Hiresh Ranjan Bhowmik,&nbsp;Anoar Jamai Masroure,&nbsp;Md Nurul Amin","doi":"10.1007/s11686-025-01043-w","DOIUrl":"10.1007/s11686-025-01043-w","url":null,"abstract":"<div><h3>Background</h3><p>Gastrointestinal (GI) parasitism remains a critical challenge in livestock health management, particularly in tropical regions where environmental conditions favor parasite proliferation. This study conducted an 8-year retrospective analysis (2017–2024) of GI parasites in dairy cattle across 11 districts in Bangladesh.</p><h3>Methods</h3><p>Data were sourced from a private veterinary diagnostic laboratory, with a focus on the prevalence, types, and trends of GI parasites as well as associated risk factors. GI parasites were identified using different coproscopic methods including direct smear, flotation, and sedimentation. Statistical analysis was performed using descriptive analysis, followed by Poisson regression.</p><h3>Results</h3><p>The most prevalent parasites identified were <i>Schistosoma</i> spp. (57.3%) and <i>Paramphistomum</i> spp. (22.6%), with notable peaks in prevalence during the years 2019 (65%) and 2021 (62%) for <i>Schistosoma</i> spp. and in 2017 (32%) for <i>Paramphistomum</i> spp. Other parasites, such as <i>Balantidium (B.) coli</i>, presented a lower but consistent prevalence (9.8%), whereas <i>Fasciola</i> spp. and <i>Haemonchus</i> spp. were identified less frequently. The analysis revealed that various factors significantly influenced parasite incidence, including seasonal variations, district-specific ecological conditions, and animal-related factors. Statistical analyses, particularly Poisson regression, demonstrated that male cattle (RR: 0.30, 95% CI: 0.230–0.401) had a lower risk of infection with <i>Schistosoma</i> spp. than females, whereas regions with abundant water bodies, such as Manikganj (RR: 11.9, 95% CI: 6.801–20.648, p &lt; 0.001), Mymensingh (RR:7.9, 95% CI: 2.243–27.78, p &lt; 0.01), and Narayanganj (RR: 6.6, 95% CI: 3.867–11.177, p &lt; 0.001), presented higher prevalence rates. Seasonal trends revealed increased prevalence rates during the winter (<i>Schistosoma</i> spp.: RR: 1.5, CI: 1.090–1.919, p &lt; 0.05; <i>Paramphistomum</i> spp.: RR: 1.03, 95% CI: 0.322–3.299; <i>B. coli</i>: RR: 1.2, 95% CI: 0.672–2.151; and <i>Fasciola</i> spp. RR: 2.5, 95% CI: 1.322–4.555), which aligns with periods of more significant water usage and potential exposure to contaminated sources.</p><h3>Conclusion</h3><p>This study underscores the need for targeted parasite control programs, particularly in high-risk areas, and advocates for improved livestock management practices and regular veterinary interventions to mitigate the economic and health impacts of GI parasites. These findings provide valuable insights for developing tailored parasite management strategies to increase the productivity and well-being of dairy cattle in Bangladesh.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocytic Activity by Apicomplexa Parasites","authors":"Wanderley de Souza","doi":"10.1007/s11686-025-01038-7","DOIUrl":"10.1007/s11686-025-01038-7","url":null,"abstract":"<div><p>Macromolecules are incorporated by eukaryotic cells through a process known as endocytosis, intensely analyzed in mammalian and yeast cells, but still lacking deep studies in pathogenic protists. Here we present what is presently known on endocytic activity carried out in some members of the Apicomplexa group (mainly concentrated in <i>Plasmodium</i> and <i>Toxoplasma</i>, but with references to <i>Eimeria</i>,<i> Babesia</i>, and <i>Theileria</i>). In most cells, endocytic activity takes place throughout the cell’s surface. In apicomplexans, it is restricted to a special surface structure known as a micropore (also called cytostome). Recent studies indicate that several proteins are in the micropore, playing a role in macromolecule uptake from the medium via budding vesicles.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal Dynamics and Epidemiology of Cutaneous Leishmaniasis in Algeria (2011–2020): One Health Insights for Targeted Interventions","authors":"Hichem Dahmani,&nbsp;Omar Salhi,&nbsp;Mustapha Nabi,&nbsp;Djamel Mokrani,&nbsp;El Aid Kaaboub,&nbsp;Imane Ouchetati,&nbsp;Nassim Ouchene,&nbsp;Nadjet Amina Khelifi Touhami","doi":"10.1007/s11686-025-01048-5","DOIUrl":"10.1007/s11686-025-01048-5","url":null,"abstract":"<div><h3>Purpose</h3><p>The epidemiology of human cutaneous leishmaniasis, a parasitic disease transmitted by sandflies, varies across regions, particularly affecting tropical and subtropical areas, with significant seasonal and demographic fluctuations. This study explores the temporal, seasonal, demographic, and spatial distribution of cutaneous leishmaniasis (CL) in the wilaya of Medea, Algeria, from 2011 to 2020.</p><h3>Methods</h3><p>The epidemiological data for this retrospective study were provided by the Prevention Service of the Directorate of Public Health (DSP) in Medea.</p><h3>Results</h3><p>In 2011, 248 cases were reported (28.62/10⁵ inhabitants). A significant decline occurred in 2012, with 123 cases (13.92/10⁵, <i>p</i> &lt; 0.05), reaching a minimum of 61 cases in 2015 (6.50/10⁵, <i>p</i> &lt; 0.01). Cases fluctuated moderately from 2016 to 2019 (<i>p</i> &lt; 0.05), but 2020 saw a sharp rise to 515 cases (49.74/10⁵, <i>p</i> &lt; 0.001), indicating a major epidemiological shift. Seasonal analysis revealed significant variations (<i>p</i> &lt; 0.001), with winter months showing the highest incidence, particularly December (433 cases) and January (269 cases). In contrast, summer months (June: 47 cases, July: 38 cases, August: 30 cases) had the lowest numbers. Demographically, children aged under 10 years old were most affected (721 cases, <i>p</i> &lt; 0.001). Men (875 cases) were more affected than women (657 cases, <i>p</i> &lt; 0.05). Geographically, southern municipalities had the highest incidence (up to 31%, <i>p</i> &lt; 0.001), while northern and eastern regions showed much lower rates. Some areas, like Chabounia, saw a dramatic increase in 2020 (80%, <i>p</i> &lt; 0.001), while Chellalat El-Adhaoura showed a steady decline.</p><h3>Conclusion</h3><p>CL in the Medea region shows significant variations over time, seasons, and geography, mainly affecting children, men, and southern areas. These findings highlight the need for stronger surveillance, targeted actions for vulnerable groups, and increased preventive measures, especially in winter, to better control the epidemic through a One Health approach.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Treatment of Lactoferrin Nanocapsules Modulates the Immune Response of Mice to a Cryptosporidiosis Infection","authors":"Ibrahim Aly,&nbsp;Waleed E. Elawamy,&nbsp;Hanan T. Hamza,&nbsp;Hany M. El-Wahsh,&nbsp;Ahmed EL-Bahiry,&nbsp;Amira Matter,&nbsp;Lamia I. Bakr","doi":"10.1007/s11686-025-01022-1","DOIUrl":"10.1007/s11686-025-01022-1","url":null,"abstract":"<div><h3>Purpose</h3><p>The current study was to evaluate the immunomodulatory impact of either lactoferrin (LF), lactoferrin nanocapsule (LF-NC), or lactoferrin conjugation with nanazoxid (NZ). Moreover, identifying drugs can effectively eliminate and successfully treat the infection with <i>Cryptosporidium</i>.</p><h3>Methods</h3><p>Sixty male Swiss albino mice were divided into six groups, each of 10 mice. G1 served as the control, and G2 was inoculated with 10⁴ oocysts. Mice were orally administered NZ (200 mg/kg b.wt.), LF (150 µg/kg b.wt.), or LF-NC (150 mg/kg b.wt./day) for a period of six days post-infection. The immunomodulatory potential and drug efficacy were assessed by fecal and intestinal oocyst counts, measuring antioxidant activities and cytokine levels in addition. Intestinal tissues were examined histopathologically.</p><h3>Results</h3><p>The cytokines TGF-β, INF-γ, and IL-10 were increased during infection and decreased following therapy. Mutually, the quantity of pathological lesions and oocysts in the ileal tissues was significantly diminished with the NZ plus LF-NC treatment. In addition, a considerable reduction of both fecal and intestinal samples, as well as a notable deterioration in oocyst counts, suggested that treatment with NZ alone or in combination with LF-NC was more effective.</p><h3>Conclusion</h3><p>LF or LF-NC was found to exert a potent immunomodulatory effect on infected mice as well as minimize pathological lesions. This therapy approaches a successful therapeutic alternative for the treatment of cryptosporidiosis with few adverse effects.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-025-01022-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Paragonimus westermani from South India","authors":"Anisha Joy,&nbsp;Anju Varghese,&nbsp;Chundayil Kalarickal Deepa,&nbsp;Karapparambu Gopalan Ajith Kumar,&nbsp;Sebasteena Peekunnel Francis,&nbsp;Aleena Iype,&nbsp;Geethu Reghu,&nbsp;Sreenidhi Nellikka Chalapron,&nbsp;Anaswara Aravindhakshan,&nbsp;George Chandy,&nbsp;Pradeep Mampillikalam,&nbsp;Hamza Palekkodan,&nbsp;Reghu Ravindran","doi":"10.1007/s11686-025-01044-9","DOIUrl":"10.1007/s11686-025-01044-9","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Paragonimus westermani</i> is the most common lung fluke infecting humans in Asia. This study aimed to molecularly characterize adult <i>P. westermani</i> from the south India (Kerala) using ITS-2, partial 28 S rDNA and partial <i>cox</i>1 gene sequences.</p><h3>Methods</h3><p>Adult flukes were recovered from the lungs of a tiger (<i>Panthera tigris</i>) (<i>n</i> = 10), a jungle cat (<i>Felis chaus</i>) (<i>n</i> = 6) and a domesticated dog (<i>Canis familiaris</i>) (<i>n</i> = 4). ITS-2, partial 28 SrDNA and partial <i>cox</i>1 gene sequences were amplified by polymerase chain reaction and a concatenated phylogenetic tree was constructed with these three marker sequences. Intra-species and inter-species genetic distances were calculated, and haplotype network analysis was performed using partial <i>cox</i>1 gene.</p><h3>Results</h3><p>Phylogenetic analysis based on the concatenated tree showed that Kerala isolates formed <i>P. westermani</i> complex with other isolates of <i>P. westermani</i> from India and from other countries. The Kerala isolates were genetically closer to metacercaria morphotype 2 of <i>P. westermani</i>. However, the genetic distance was higher among Indian isolates, indicating significant genetic variation among <i>P. westermani</i> within the complex. In addition, India also exhibited the highest number of haplotypes (<i>n</i> = 8).</p><h3>Conclusions</h3><p>Concatenated phylogenetic analysis of the Indian isolates of <i>P. westermani</i> show them to form <i>P. westermani</i> complex. The parasite exhibits high genetic distance with a large number of haplotypes. Further epidemiological studies are needed to understand its genetic variability. No sub-populations have been recognized in <i>P. westermani</i> based on the presented molecular data.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Species of Oochoristica Lühe, 1898 (Cestoda: Linstowiinae) from Sceloporus ochoterenae (Reptilia: Sauria: Phrynosomatidae) in Central Mexico: Approach to the Phylogenetic Relationships of the Genus with Molecular Evidence","authors":"Andrés Velázquez-Brito,&nbsp;Luis García-Prieto,&nbsp;Uriel Garduño-Montes de Oca,&nbsp;Virginia León-Règagnon","doi":"10.1007/s11686-025-01046-7","DOIUrl":"10.1007/s11686-025-01046-7","url":null,"abstract":"<div><h3>Purpose</h3><p>To describe a new species of <i>Oochoristica</i> Lühe, 1898, an anoplocephalid cestode included in Linstowiinae (Cyclophyllidea), parasitizing <i>Sceloporus ochoterenae</i> Smith in Zoyatepec, Guerrero, Mexico.</p><h3>Methods</h3><p>All specimens were fixed with 4% hot formalin. A morphologic study was made using stained material under light microscopy. Some specimens of a second species (collected in <i>Sceloporus grammicus</i> from La Malinche, Tlaxcala, fixed in 100% ethanol, were also stained and studied morphologically; others were subject of DNA sequencing and phylogenetic analysis.</p><h3>Results</h3><p>A new species of this genus is described of the intestine of an endemic lizard <i>S. ochoterenae</i>. <i>Oochoristica guerreroensis</i> n. sp. shares a distinctive morphological feature (mature proglottids wider than long) with only 13 species of genus. However, <i>O. guerreroensis</i> n. sp. can be differentiated by: strobilar size (4.29–7.62 mm), number of testes (48–55), shape of vitellaria (ovoid) and number of ovarian sublobes (3–5), as well as by their geographical distribution (Neotropical region of Mexico), and type of host. Based on molecular evidence of nuclear 28<i>S rDNA</i> gene, we analyze for the first time the phylogenetic position of one species of <i>Oochoristica</i> (<i>O. scelopori</i>) within Anoplocephalidae as well as their relationships with other genera of Cyclophyllidea.</p><h3>Conclusions</h3><p>The new species is the first described in association with a phrynosomatid lizard in the Neotropical realm, and the ninth recorded in Mexico. Molecular data obtained in our study seem to confirm the independence <i>Oochoristica</i> and <i>Mathevotaenia</i>.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-025-01046-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Microcotyle tazeroutiae n. sp. (Monogenea: Microcotylidae) from the Gills of the Boarfish Capros aper Linnaeus, 1758 (Teleostei: Caproidae) Off the Algerian Coast, Western Mediterranean","authors":"Amira Meriem Rebah,&nbsp;Delphine Gey,&nbsp;Zouhour El Mouna Ayadi","doi":"10.1007/s11686-025-01032-z","DOIUrl":"10.1007/s11686-025-01032-z","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Cultivation of Acanthamoeba spp. Isolated from Environmental Samples Using Nanocomposite and Leech Saliva on Non-Nutrient Agar","authors":"Alican Bilden,&nbsp;Erdal Ertaş,&nbsp;Merve Kahraman,&nbsp;Servet Tural,&nbsp;Bilsen Tural,&nbsp;Muttalip Çiçek","doi":"10.1007/s11686-025-01053-8","DOIUrl":"10.1007/s11686-025-01053-8","url":null,"abstract":"<div><h3>Objective</h3><p><i>Acanthamoeba</i> spp. are microscopic single-celled protozoa commonly found in the environment, particularly in soil, water sources, and dust. These parasites are associated with serious infections such as <i>Acanthamoeba</i> keratitis and granulomatous amebic encephalitis. Recent epidemiological studies have highlighted a significant increase in <i>Acanthamoeba</i> keratitis cases. Current treatment methods are generally effective only in the early stages and show limited success when applied late, emphasizing the urgent need for more effective therapeutic and cultivation approaches. </p><p>The laboratory cultivation of <i>Acanthamoeba</i> spp. is traditionally performed using axenic or monoxenic cultures. However, these methods have notable drawbacks, including the loss of virulence, reduced encystment capability, errors in bacterial inoculation, and time-consuming procedures. To address these limitations, nanotechnological approaches have been proposed. Nanotechnology offers innovative solutions for developing new drug formulations and diagnosing, preventing, and treating various diseases.</p><h3>Methods</h3><p>In this study, we developed humic acid-coated magnetic nanocomposites capable of adhering to the cyst wall of <i>Acanthamoeba</i> spp. without causing damage. Experimental results demonstrated that these nanocomposites bind with high affinity to macromolecules on the cyst wall, facilitating the aggregation of parasites in the pellet. Additionally, biologically enriched leech saliva was incorporated into the culture medium to enhance the growth rate. Leech saliva provides a rich source of organic matter and bioactive molecules that promote cell division. The addition of leech saliva resulted in a significant increase in the growth rate of <i>Acanthamoeba</i> spp., with maximum growth density observed at 120 h.</p><h3>Results</h3><p>These findings indicate that humic acid-coated magnetic nanocomposites and leech saliva -enriched culture media offer a promising alternative to conventional methods for the rapid and efficient cultivation of <i>Acanthamoeba</i> spp. Our study concludes that humic acid-coated magnetic nanocomposites effectively concentrate parasites, increasing their quantitative density, while leech saliva provides a nutrient-rich environment that stimulates trophozoite feeding and division.</p><h3>Conclusion</h3><p>This study is noteworthy for presenting an innovative and effective method for the rapid laboratory cultivation and potential treatment of <i>Acanthamoeba</i> spp.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-025-01053-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}