Acta Parasitologica最新文献

{"title":"Investigation of Betaine and Vaccine Efficacy for Coccidiosis Prevention in Broilers.","authors":"Asude Gulce Oryasin, Hasan Eren","doi":"10.1007/s11686-024-00967-z","DOIUrl":"https://doi.org/10.1007/s11686-024-00967-z","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to assess the anticoccidial effects of betaine and a vaccine compared to monensin sodium in experimentally induced coccidiosis in broiler chickens.</p><p><strong>Methods: </strong>600 day-old broiler chickens (Ross 308) were randomly assigned to five groups, each with four replicates of 30 birds. While the control group received a basal diet, two experimental groups received basal diet supplemented with either 100 mg/kg monensin sodium or 2.0 g/kg betaine. The remaining experimental groups received a commercial coccidiosis vaccine alone or in combination with betaine. All chickens were challenged with sporulated field-mixed Eimeria species at 20 days of age.</p><p><strong>Results: </strong>Throughout the study, vaccinated birds showed superior performance in terms of body weight gain (BWG) and feed conversion ratio (FCR) compared to other anticoccidial treatments (P < 0.05), followed by betaine, vaccine + betaine, and monensin treatments in descending order. While all anticoccidial regimens significantly reduced fecal oocyst output only at the beginning of the observation period, this effect diminished thereafter (P < 0.05). Supplementation with monensin and vaccination against coccidiosis significantly decreased small intestine weight compared to untreated controls (P < 0.01), with a numerical decrease observed in chicks fed betaine-supplemented diets and treated with the combination of betaine and vaccine.</p><p><strong>Conclusion: </strong>Notably, there has been no prior study comparing betaine with monensin sodium and a coccidiosis vaccine. These findings suggest that dietary betaine supplementation and a commercial coccidiosis vaccine containing mixed Eimeria spp. may offer benefits in controlling coccidiosis, presenting viable, cost-effective, sustainable, and safe alternatives to conventional ionophore anticoccidials, with added benefits of no residue and reduced resistance hazards for both animal and human consumption.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"25"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Survey of Protist Enteroparasites in Bats (Order Chiroptera) from Portugal.","authors":"Pedro López-López, Andreia V S Cruz, Sérgio Santos-Silva, Antonio Rivero-Juárez, Hugo Rebelo, João R Mesquita","doi":"10.1007/s11686-024-00985-x","DOIUrl":"https://doi.org/10.1007/s11686-024-00985-x","url":null,"abstract":"<p><strong>Purpose: </strong>Bats constitute 20% of all mammal species, playing a vital role in ecosystem health as pollinators, seed dispersers, and regulators of insect populations. However, these animals can also be reservoirs for infectious agents, including viruses, bacteria, and enteroparasites such as Cryptosporidium spp., Giardia duodenalis, and Balantioides coli, raising questions about their role in the epidemiology of these agents. Our study analyses bat faecal samples from Portugal with the aim of assessing the prevalence, distribution and diversity of enteroparasitic protozoa.</p><p><strong>Methods: </strong>We conducted a retrospective study that included 380 bat faecal samples collected between 2014 and 2018 in northern and central Portugal.</p><p><strong>Results: </strong>In our study, a Cryptosporidium spp. prevalence of 3.2% was identified, with genetic diversity observed and strains grouped with known bat genotypes. Giardia duodenalis was detected in 0.5% of the samples, exhibiting distinct genetic characteristics that may suggest a potential new assembly group encompassing bats and rodents. Finally, B. coli was detected in 0.26% of samples, representing the first observation of this ciliate in bats, with the identified genetic variant belonging to genotype B.</p><p><strong>Conclusion: </strong>Our results provide valuable molecular epidemiological insights that underscore the importance of bats in the epidemiology of these enteroparasites. Furthermore, this is the first report of Cryptosporidium spp. and G. duodenalis in bat faeces samples from Portugal, and the first study worldwide to show that bats can shed B. coli in their faeces.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"24"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Molecular Characterization of Two Species of Ligophorus (Monogenea: Ancyrocephalidae) in Mullets from the Yucatán Peninsula, with Comments on the Geographical Distribution of L. mediterraneus.","authors":"Leopoldo Andrade-Gómez, Reinaldo José da Silva, Gerardo Pérez-Ponce de León","doi":"10.1007/s11686-024-00953-5","DOIUrl":"https://doi.org/10.1007/s11686-024-00953-5","url":null,"abstract":"<p><strong>Background: </strong>Ligophorus Euzet and Suriano, 1977 is a specious genus of ancyrocephalid monogeneans parasitizing mullets around the world, with most species distributed in the western Pacific and the Mediterranean Sea. Only nine out of the 62 species in the genus have been reported from the Americas, and from them, only two have been sequenced.</p><p><strong>Methods: </strong>We analyzed two species of Mugil (L.) from Northern Yucatán Peninsula. Specimens of Ligophorus were sampled from the gills of their hosts. The morphology of the specimens was examined. In addition, 28S and ITS rDNA sequences were obtained and compared with previous sequences downloaded from GenBank.</p><p><strong>Results: </strong>We discovered two species of Ligophorus using morphological and molecular characters, L. mediterraneus, parasitizing the stripped mullet Mugil cephalus off the coast of Celestún, and L. yucatanensis, parasitizing the silver mullet M. curema in four coastal lagoons. Sequence data of the latter species are reported for the first time.</p><p><strong>Conclusion: </strong>Our findings showed that two species of Ligophorus occur in mugilids of the Yucatán Peninsula. One represents a widely distributed marine species with records in the Mediterranean Sea and the Yucatán Peninsula, whereas the second one, L. yucatanensis, represents an endemic species restricted to coastal lagoons of the Yucatán Peninsula.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"30"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Role of Sanguinarine as an Inhibitor of Leishmania PP2C in the Induction of Apoptosis.","authors":"M Ornelas-Cruces, A R Escalona-Montaño, N Salaiza-Suazo, S Sifontes-Rodríguez, M M Aguirre-García","doi":"10.1007/s11686-024-00977-x","DOIUrl":"https://doi.org/10.1007/s11686-024-00977-x","url":null,"abstract":"<p><p>Leishmania spp. cause a wide range of human diseases, localized skin lesions, mucocutaneous and visceral infections. In the present study, the aim was to investigate the potential role of sanguinarine as a specific inhibitor of Leishmania PP2C that can induce apoptosis in the parasite. The results demonstrated that sanguinarine inhibits, in a dose-dependent mode at 72 h, the growth and phosphatase activity of both Leishmania major and Leishmania mexicana promastigotes. Therefore, all assays were performed from this time period onwards. TUNEL assay was used to identify apoptosis and indicated apoptosis in L. major and L. mexicana promastigotes. Similarly, Western blot assay showed that PARP, a DNA damage indicator molecule, was present in L. major and L. mexicana promastigotes incubated with the inhibitor. In addition, differential expression of the proapoptotic protein Bax and the antiapoptotic protein Bcl-2 was observed in both Leishmania species. Finally, the protein phosphatase PP2C expression was not affected, whereas p38 MAPK phosphorylation was increased in L. major promastigotes than in L. mexicana promastigotes. Therefore, sanguinarine proved to be an inhibitor of the growth and PP2C enzymatic activity of L. major and L. mexicana promastigotes, and with it, this inhibition induced apoptosis.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"35"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Propolis and Royal Jelly in Encephalitozoon Intestinalis Infection: An in Vitro Study.","authors":"Derya Gül Gülpinar, Zübeyda Akın Polat, Ülfet Çetinkaya","doi":"10.1007/s11686-024-00956-2","DOIUrl":"https://doi.org/10.1007/s11686-024-00956-2","url":null,"abstract":"<p><strong>Purpose: </strong>Encephalitozoon intestinalis is an obligate intracellular microsporidian fungus that causes severe gastrointestinal infections, particularly in immunocompromised individuals. Propolis (PROP), a resinous substance derived from bees, has antimicrobial, anti-inflammatory and antioxidant properties, while royal jelly (RJ) has immunomodulatory, antioxidant and antimicrobial activities. The aim of this study was to investigate the therapeutic potential of PROP and RJ against E. intestinalis.</p><p><strong>Methods: </strong>The phenolic composition of PROP was analysed by high-performance liquid chromatography with diode array detection, and the chemical components of RJ were evaluated according to ISO12824 standards. The cytotoxicity of PROP and RJ on HEK-293 cells was evaluated using the XTT assay. The three highest non-cytotoxic concentrations of each sample were tested for their effects on E. intestinalis spores by qRT-PCR. Trichrome-stained photomicrographs were used to assess spore density in HEK-293 cells treated with PROP and RJ.</p><p><strong>Results: </strong>PROP analysis revealed flavonoids such as quercetin, kaempferol, pinocembrin and galangin, as well as phenolic acids such as caffeic and cinnamic acids, known for their bioactive properties. RJ contained mainly proteins, lipids, carbohydrates and sugars, reflecting its role as a nutritionally and biologically active substance. According to the results of this first study evaluating the effect of PROP and RJ on E. intestinalis, all concentrations evaluated in the study showed a significant inhibitory effect on the growth of E. intestinalis spores compared to the control group.</p><p><strong>Conclusion: </strong>In conclusion, we believe that PROP and RJ should be considered as an alternative option in the development of antimicrosporidial drugs due to their potential medicinal and pharmaceutical properties.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"26"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laminated Layer Extract from Echinococcus Granulosus cyst Attenuates Ocular Damages and Inflammatory Responses in an Experimental Autoimmune Uveitis Model.","authors":"Sara Djebbara, Houda Belguendouz, Imene Soufli, Leila Hannachi, Fahima Ameur, Sara Benazzouz, Sarra Benkhelifa, Malika Terrahi, Karima Achour, Zine-Charaf Amir, Manel Amri, Chafia Touil-Boukoffa","doi":"10.1007/s11686-024-00944-6","DOIUrl":"https://doi.org/10.1007/s11686-024-00944-6","url":null,"abstract":"<p><strong>Purpose: </strong>Since extract of the laminated layer (LL) from E. granulosus showed immuno-modulatory effects in vitro and in vivo, we sought to determine its effect on the onset, development, and evolution of experimental auto-immune uveitis (EAU). The latter is a model of some human diseases with ocular inflammation that can cause blindness.</p><p><strong>Methods: </strong>E. granulosus LL extract was either injected before EAU induction for the pretreated group or later for treated group. Ocular exploration was made by retinal histological and immunohistological (CD86, CD4, CD8) analysis. Myeloperoxidase (MPO), Superoxide dismutase (SOD), Catalase enzymatic activities (CAT), and Malondialdehyde (MDA), Nitric oxide (NO), Urea, and TNF-α levels were measured in plasma.</p><p><strong>Results: </strong>LL injection attenuated retinal histological damage and reduced cells infiltration. Also, LL decreased systemic inflammatory and oxidative markers as well as TNF-α production and increased antioxidant parameters.</p><p><strong>Conclusions: </strong>Interestingly, we observed a protective effect of E. granulosus LL extract during EAU. LL appears to ameliorate retinal damage by down-regulating inflammatory responses. Our results support LL immunomodulatory effects during autoimmune diseases and offer a promising prospect for helminthic therapy during autoimmune uveitis.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"34"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colchicine: A Dual Therapeutic Target for Trichinellosis.","authors":"Enas Fakhry Abdel Hamed, Afaf A Taha, Shereen M Abdel Ghany, Al-Sayed R Al-Attar, Eman M Fawzy","doi":"10.1007/s11686-024-00979-9","DOIUrl":"https://doi.org/10.1007/s11686-024-00979-9","url":null,"abstract":"<p><strong>Purpose: </strong>Trichinellosis affects around 11 million people globally. Treatments for this medical condition are limited by adverse effects and resistance, emphasising the importance of effective and safe therapies. Consequentially, we sought to study colchicine's synergistic effects with atorvastatin or acetazolamide in the treatment of Trichinella spiralis (T. spiralis)-infected mice.</p><p><strong>Methods: </strong>Seventy mice were evenly divided into two groups (a and b) of 35 each. During the intestinal phase, group (a) began therapy on the second day post-infection (dpi) and lasted four days. Group (b) had treatment for four weeks during the muscle phase, beginning on the 12th dpi. While the other five infected groups received atorvastatin, colchicine, acetazolamide, a combination of acetazolamide and colchicine, or none, one group of infected mice received no treatment at all as a negative control. The efficacy was assessed by parasite count, histopathology and scanning electron microscopy.</p><p><strong>Results: </strong>Our data revealed that the combination treatment lowered T. spiralis adult worm and larvae counts in infected animals. Moreover, it restored the normal intestinal and muscular architecture, reduced edema, and alleviated inflammation, as demonstrated by reduced inflammatory infiltrate. Scanning electron microscopic examination of adults and larvae verified our findings.</p><p><strong>Conclusion: </strong>Adjuvant treatment with colchicine as an antifibrotic can help treat muscle trichinellosis by reducing the production of fibrous tissue. This might help to enhance treatment results by enabling the admission of larvicidal medications and, as a result, reducing the number of larvae in the muscle, which together form the basis of pathology and can be debilitating for the patient.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"42"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhanterium Epapposum Essential Oil and Its Primary Compounds Control Infection, Inflammation, and Serum Electrolyte Imbalance in Mice with Giardiasis.","authors":"Zafer Saad Alshehri, Faez Falah Alshehri","doi":"10.1007/s11686-024-00974-0","DOIUrl":"https://doi.org/10.1007/s11686-024-00974-0","url":null,"abstract":"<p><strong>Purpose: </strong>The present experimental study seeks to evaluate the in vitro and in vivo effects, as well as the potential mechanisms of action, of Rhanterium epapposum essential oil (REE) and its main constituents against Giardia lamblia infection.</p><p><strong>Methods: </strong>The analysis of REE was performed using the Gas Chromatography-Mass Spectrometry (GC-MS) detector. The in vitro effects of REE and its main constituents on viability of G. lamblia cysts and the human intestinal epithelial cell line, as well as their effects on plasma membrane permeability, the reactive oxygen species (ROS) generation, and trophozoite adherence were assessed by MTT assay. Effects of oral administration of REE and its main constituents at doses 10 and 20 mg/kg on the number of Giardia cysts, the serum level of electrolytes of sodium (Na+) and potassium (K+), as well as the gene expression of inflammatory genes of TNF-α, IL-1β, IL-10, NF-κB p65, and Toll-like receptor 4 (TLR4)) were assessed.</p><p><strong>Results: </strong>The 50% inhibitory concentrations (IC50) value of REE, MC, CP, LN and MTZ against G. lamblia cysts was 26.7, 32.1, 35.3, 45.1, and 42.1 µg/mL, respectively; indicated that REE, MC, and CP displayed high antigiardial effects in comparison with MTZ. The obtained 50% cytotoxic concentration (CC50) value of REE, MC, CP, LN, and MTZ on HIEC-6 cells was 279.1, 363.4, 394.2, 478.3, and 422.1 µg/mL, respectively. REE and its main compounds significantly (p < 0.001) increased the plasma membrane permeability and ROS generation in Giardia trophozoites, while, reduced the Giardia trophozoites adherence. In vivo, REE, MC, CP, and LN mainly at the dose of 20 mg/kg considerably reduced the number and viability of Giardia cysts, modulated the serum level of electrolytes Na and K in the infected mice compared with the mice treated with MTZ (P < 0.01); whereas inhibited the inflammation cytokines in the infected mice compared with the mice treated with MTZ (p < 0.001).</p><p><strong>Conclusion: </strong>The current investigation showed that R. epapposum essential oil and its main compounds controlled the Giardia infection in mice by inhibiting inflammation, and serum electrolyte imbalance. After confirmation in further studies, these compounds may be considered for development as a novel therapeutic option for the treatment of giardiasis.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"37"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of TNF-α, VEGF-A and Microvessel Density in Cerebral Alveolar Echinococcosis and Their Correlation with Perilesional Brain Edema.","authors":"Wuerken Wumier, Alimasi Abulizi, Pengfei Wu, Najiahai Jinsihan, Yongxin Wang, Serick Duysenbi, Zengliang Wang","doi":"10.1007/s11686-024-00943-7","DOIUrl":"https://doi.org/10.1007/s11686-024-00943-7","url":null,"abstract":"<p><p>Alveolar echinococcosis (AE) is an infrequent zoonosis caused by Echinococcus multilocularis with a high degree of disability and mortality. Metastatic cerebral alveolar echinococcosis (CAE) is very rare and the lesions could lead to severe perilesional brain edema (PLBE) and subsequent uncontrollable intracranial hypertension. In this study, we sought to determine the expression of edema-associated factors in CAE lesions and their associations with PLBE. We retrospectively evaluated the clinical data of 18 CAE patients who received craniotomy. Severity of PLBE was described by edema index (EI). Archived specimens were processed for immunohistochemistry to detect tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor A (VEGF-A) and microvessel density (MVD) in CAE lesions. Expression intensity of CAE lesions was quantified by integral optical density (IOD) or count and was compared to the control group. The results showed TNF-α and VEGF-A were significantly expressed in CAE lesions (p < 0.001), their levels were positively correlated with PLBE (TNF-α: r = 0.701, p = 0.001; VEGF-A: r = 0.803, p < 0.001). The MVD of CAE lesions had a similar expression with normal brain tissue, and it was positively correlated with PLBE and VEGF-A (PLBE: r = 0.849, p < 0.001; VEGF-A: r = 0.687, p = 0.002). In conclusion, we speculated the upregulation of TNF-α and VEGF-A induced the formation of PLBE. Besides, though there was no extra increase of MVD, it was still regulated by VEGF-A and provided a better anatomical basis for the formation of PLBE and further promoted it.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"33"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hennegoides Africanus sp. nov. from Kadey River in Cameroon.","authors":"Nelly Ornelle Onana-Ateba, Guy Benoît Lekeufack-Folefack, Stephane Fadanka, Gloria Berinyuy-Tata, Abraham Fomena","doi":"10.1007/s11686-024-00976-y","DOIUrl":"https://doi.org/10.1007/s11686-024-00976-y","url":null,"abstract":"<p><strong>Purpose: </strong>Fish are susceptible to various parasitic infections, with Myxozoa emerging as a major group. A taxonomic study of Myxozoa is essential for the rapid diagnosis of species potentially responsible for epizootic diseases.</p><p><strong>Methods: </strong>The studied fish was collected from the Kadey River, a tributary of the Sangha River in the Congo Basin in Cameroon, and parasitologically dissected. Cysts from gill samples were examined morphologically and molecularly to assess myxozoan diversity and phylogenetic relationships were determined based on SSU rDNA.</p><p><strong>Results: </strong>Infection by Hennegoides africanus sp. nov., found in gill filaments of 60% of C. gibbosus. Infection characterized as intrafilamental-epithelial type. Myxospore body vaulted on one side, measured 13.5 (12.4-14.8) × 2.1 (1.9-2.5) µm. Caudal appendages measured 31.3 (25.4-34.93) µm, made of thick and filamentous segments. Two polar capsules elongated, differing in size and position; smaller capsule near anterior end measured 2.8 (2.18-3.54) × 0.9 (0.7-1.1) µm, while larger, located posteriorly, measured 3.8 (3.21-4.45) × 1.4 (1.2-1.7) µm. The SSU rDNA gene sequence of Hennegoides africanus sp. nov. does not match any sequences in GenBank and is closely associated with two undetermined species of Henneguya and Myxobolus infecting the gills of Characiformes in Africa.</p><p><strong>Conclusion: </strong>Morpho-molecular evidence indicates that Hennegoides africanus sp. nov. is a new species to science and the first reported member of its genus in Africa.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"39"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}