Acta Parasitologica最新文献

{"title":"Flies as Carriers of Gastrointestinal Protozoa of Interest in Public Health in the Northeast of Brazil","authors":"João Victor Batista dos Santos,&nbsp;Anna Luiza Hora dos Santos,&nbsp;André Mota Alves,&nbsp;Matheus Resende de Oliveira,&nbsp;Kalina Maria de Medeiros Gomes Simplicio,&nbsp;Lorena Maciel Santos Silva,&nbsp;João Victor de Jesus,&nbsp;Michelle Evangelista Soares,&nbsp;Ana Cinthia Santos da Costa,&nbsp;Weslania Sousa Inacio da Silva,&nbsp;Cláudia Moura de Melo,&nbsp;Rubens Riscala Madi,&nbsp;Victor Fernando Santana Lima","doi":"10.1007/s11686-024-00935-7","DOIUrl":"10.1007/s11686-024-00935-7","url":null,"abstract":"<div><h3>Purpouse</h3><p>This study aimed to assess the influence of the presence of synanthropic flies in food preparation environments on the transmission of potentially zoonotic gastrointestinal protozoa.</p><h3>Methods</h3><p>Flies were captured using a glass containing water, fruits, and pieces of protein.</p><h3>Results</h3><p>Approximately 260 flies from four different species were captured: <i>Musca domestica</i> (76.92%; 200/260), <i>Cochliomyia hominivorax</i> (17.31%; 45/260), <i>Lucilia cuprina</i> (3.85%; 10/260), and <i>Hydrotaea aenescens</i> (1.92%; 5/260). Protozoa were identified through microscopic analysis after macerating these arthropods contained in their respective eppendorfs, using two techniques, namely Mini-FLOTAC^® and centrifugal sedimentation with Ziehl–Neelsen staining. The analysis revealed a concerning scenario regarding the epidemiological chain of gastrointestinal protozoa in Sergipe, Northeast Brazil. The positivity rate was 26.92% (14/52) of the pools, with 100.00% (5/5) in Aracaju, 30.00% (3/10) in Nossa Senhora do Socorro, 27.27% (3/11) in Nossa Senhora da Glória, 12.50% (2/16) in Carmópolis, and 10.00% (1/10) in Nossa Senhora das Dores. Two species of protozoa were identified, namely <i>Cryptosporidium</i> spp. (23.08%; 12/52) and <i>Entamoeba</i> spp. (9.62%; 5/52). The involvement of two fly species in the maintenance of the life cycle of these protozoa was noticeable, specifically <i>M. domestica</i> (27.50%; 11/40) and <i>C. hominivorax</i> (22.22%; 2/9).</p><h3>Conclusions</h3><p> Counties analyzed had an human development index (HDI) considered medium; however, they still faced socioeconomic problems such as absence of sanitation systems, waste accumulation in the streets, and illegal waste disposal, which could favor the proliferation of these vectors and the spread of these gastrointestinal protozoa.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2055 - 2063"},"PeriodicalIF":1.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: The Protective Role of Toll-Like Receptor Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis","authors":"Ibrahim Aly,&nbsp;Essam H. Ibrahim,&nbsp;Rabab S. Hamad,&nbsp;Hoda E. L. Sayed,&nbsp;Sama M. N. Attiyah,&nbsp;Wafaa E-Komy,&nbsp;Hamed A. Ghramh,&nbsp;Ali Alshehri,&nbsp;Khalid M. Alsyaad,&nbsp;Mohammed Alshehri,&nbsp;Mona Kilany,&nbsp;Kareem Morsy,&nbsp;Attalla F. El-kott,&nbsp;Ramadan Taha","doi":"10.1007/s11686-024-00936-6","DOIUrl":"10.1007/s11686-024-00936-6","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2097 - 2097"},"PeriodicalIF":1.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Modern Morphological and Molecular Description of Saccocoelium Cephali Larvae Stages (Digenea: Haploporidae) from the Black Sea","authors":"Yuliya V. Belousova,&nbsp;Dmitry M. Atopkin","doi":"10.1007/s11686-024-00934-8","DOIUrl":"10.1007/s11686-024-00934-8","url":null,"abstract":"<div><h3>Purpose</h3><p>Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from the Black and Medditeranean Seas were known. The present research is the first report of haploporid cercariae <i>S. cephali</i> from the gastropod <i>Hydrobia acuta</i> inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from the Haploporidae family.</p><h3>Methods</h3><p><i>Hydrobia acuta</i> molluscs were collected in the estuary of the Chernaya River (the Black Sea) during 2011–2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species, and molecular comparisons were made of the subunit ribosomal DNA with other Haploporidae species.</p><h3>Results</h3><p>The haploporid parthenitae were found in the gonads of <i>Hydrobia acuta</i> molluscs. Most of the morphological characteristics of the specimens investigated agree with those of <i>Saccococoelium </i>sp<i>.</i> and are closest to those of <i>S. cephali</i> by shape and length of the body, ratio of suckers, ratio of tail length to body, shape, and size of the pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens from the Black Sea were within the hapolporine clade and closely related to <i>S. cephali</i> from Spain.</p><h3>Conclusion</h3><p>Morphological characteristics of cercariae emerging from <i>Hydrobia acuta</i> from the Black Sea and the analysis of partial 28S rDNA sequences support the conspecificity of the parasite with <i>S. cephali</i> mature worms from the Mediterranean Sea.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1919 - 1925"},"PeriodicalIF":1.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Liver and Jejunal Abscesses Due to Fasciola Flat Worm: An Uncommon Case Report from Iran","authors":"Shahriar Alian,&nbsp;Shadi Shayesteazar,&nbsp;Eissa Soleymani,&nbsp;Farzad Yazdani,&nbsp;Mohammad Reza Azimi,&nbsp;Mahdi Fakhar","doi":"10.1007/s11686-024-00931-x","DOIUrl":"10.1007/s11686-024-00931-x","url":null,"abstract":"<div><h3>Introduction</h3><p>Fascioliasis is a zoonotic condition precipitated by the trematode known as <i>Fasciola</i> spp. This parasite affected almost 2.4 to 17 million individuals in the world. The occurrence of liver abscesses is an infrequent phenomenon, potentially arising as a secondary outcome of cholangitis or hepatic inflammation. In this study, we have reported a case of a hepatic abscess induced by <i>Fasciola</i> spp.</p><h3>Case Report</h3><p>A 29-year-old male presented with generalized abdominal discomfort, primarily concentrated in the right upper quadrant, which had progressively worsened over the past seven months. The patient did mention a weight loss of approximately 10 kg during the course of their illness, along with increasing fatigue. Eosinophilia was seen too (35%). The stool exam test was negative. An enteroscopy was conducted, revealing no pathological abnormalities. Following this, the surgeon prescribed a surgical biopsy of the mass, which yielded a diagnosis of multiple eosinophilic granulomatous abscesses within the jejunal wall and mesentery. The <i>Fasciola</i> IgG test returned a positive result. After of follow-up, the patient fully recovered.</p><h3>Conclusion</h3><p>Fascioliasis should be considered in the differential diagnoses of liver abscesses. Because of earlier diagnosis, can be avoided of invasive diagnostic tests and therapeutic interventions can be started earlier.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2051 - 2054"},"PeriodicalIF":1.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Changes in the Tegument and Tissues of Fasciola hepatica Adults and Their Eggs Due to the Effect of an Ethyl Acetate Extract of Artemisia ludoviciana Nutt. spp Mexicana","authors":"Alonso Ezeta-Miranda,&nbsp;José Guillermo Avila-Acevedo,&nbsp;Adriana Montserrat Espinosa-González,&nbsp;José del Carmen Benítez-Flores,&nbsp;Gerardo Francisco-Marquez,&nbsp;Yolanda Vera-Montenegro","doi":"10.1007/s11686-024-00933-9","DOIUrl":"10.1007/s11686-024-00933-9","url":null,"abstract":"<div><h3>Purpose</h3><p>The objective of the present work was to evaluate the effect of an ethyl acetate extract of <i>Artemisia ludoviciana</i> on the viability of adult <i>Fasciola hepatica</i> parasites and eggs.</p><h3>Methods</h3><p>The collection of plant material was performed as described in previous reports. The dried material was macerated with ethyl acetate. Ovicidal assays were performed at concentrations of 100, 200, 300, 400 and 500 mg/L <i>A. ludoviciana</i> extract. Bioassays of fasciolicidal efficacy in adult specimens of <i>F. hepatica</i> were performed at extract concentrations of 125, 250, 375 and 500 mg/L. The effects of triclabendazole, a reference drug, and artemisinin were also evaluated.</p><h3>Results</h3><p>The ovicidal effectiveness of the extracts obtained after 16 h of incubation at concentrations of 100, 200, 300, 400 and 500 mg/L was 48%, 52%, 87%, 89% and 92%, respectively (<i>p</i> &lt; 0.05), and the fasciolicidal efficiencies during the first 24 h post-treatment ranged from 82 to 100% (<i>p</i> &lt; 0.05). In both cases, scanning electron microscopy revealed damage to the shells of the eggs treated with the extract, compromising their stability. In adult fasciolae, alterations to the integument that resulted in its erosion and detachment were observed. Histopathological studies of the affected specimens revealed damage to the tegumentary and subtegumentary cells and alterations in the ovaries, testicles and intestine. This damage was more severe after treatment with the extract than after treatment with the other compounds.</p><h3>Methods</h3><p>Extract of <i>A. ludoviciana</i> causes damage to the tegument, intestine, ovaries, testes and eggs of <i>F. hepatica</i>.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2016 - 2030"},"PeriodicalIF":1.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00933-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species of Heligmonellidae (Nematoda) in Euryoryzomys russatus (Rodentia: Sigmodontinae) in the Atlantic Forest of Northeast Brazil","authors":"Natália Alves Costa,&nbsp;Rosana Gentile,&nbsp;Martin R. Alvarez,&nbsp;Arnaldo Maldonado Junior","doi":"10.1007/s11686-024-00928-6","DOIUrl":"10.1007/s11686-024-00928-6","url":null,"abstract":"<div><h3>Background</h3><p>The genus <i>Stilestrongylus </i>(Freitas, Lent, and Almeida, 1937) is defined by having 24 or more subequal cuticular ridges, an asymmetrical caudal bursa, and a hypertrophied genital cone. It comprises 25 recognized species, mainly parasitizing cricetids, and shows significant diversification linked to the evolution of its hosts. <i>Stilestrongylus magnumspiculum </i>n. sp. is a newly described nematode species identified in the small intestine of the rodent <i>Euryoryzomys russatus</i> (Cricetidae, Sigmodontinae) from the Atlantic Forest of Northeast Brazil. This study aims to characterize the new species and its prevalence in host populations.</p><h3>Methods</h3><p>A total of 43 <i>Euryoryzomys russatus </i>individuals were collected and analyzed for parasitic infections. Morphological features of <i>Stilestrongylus magnumspiculum </i>n. sp. were examined, focusing on the number of ridges, synlophe characteristics, caudal bursa type, lobe hypertrophy, and the spicule length to body length ratio.</p><h3>Results</h3><p>The new species exhibited a prevalence of 21% among the sampled hosts. Distinctive morphological features were observed, including:the synlophe structure exhibited specific ridge patterns that differentiate it from other species within the genus; the caudal bursa was identified as a type 2-2-1; hypertrophy of the right lobe was observed, indicating notable morphological adaptation; rays 4 and 5 were found to be equivalent in size and diverged at the distal end; the spicule length to body length ratio (SpL/BL) was measured at 30–36%, representing the largest ratio recorded for the genus. In comparison, other <i>Stilestrongylus </i>species displayed the following ratios: <i>S. rolandoi </i>(21–33%), <i>S. lanfrediae</i> (25–29%), <i>S. kaaguyporai</i> (20–24%), <i>S. inexpectatus </i>(20–23%), and <i>S. stilesi </i>(26–28%).</p><h3>Conclusions</h3><p>This report presents <i>Stilestrongylus magnumspiculum </i>n. sp. as a new species of nematode, contributing to the diversity of the genus <i>Stilestrongylus</i>. The findings underscore the importance of studying host-parasite interactions within the Atlantic Forest ecosystem and provide a basis for future ecological and parasitological research.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1911 - 1918"},"PeriodicalIF":1.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00928-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species of Anacanthorus (Dactylogyridae, Anacanthorinae) Parasitizing Gills of Hoplias aff. malabaricus (Bloch, 1794) (Characiformes, Erythrinidae) from the Caatinga Domain","authors":"Priscilla de Oliveira Fadel Yamada,&nbsp;Maria Fernanda Barros Gouveia Diniz,&nbsp;Wallas Benevides Barbosa de Sousa,&nbsp;Fabio Hideki Yamada,&nbsp;Marcos Tavares-Dias","doi":"10.1007/s11686-024-00864-5","DOIUrl":"10.1007/s11686-024-00864-5","url":null,"abstract":"<div><h3>Introduction</h3><p><i>Anacanthorus silvoi</i> n. sp. (Dactylogyridae, Anacanthorinae) is described from the gills of <i>Hoplias</i> aff. <i>malabaricus</i> (Bloch, 1794) from the Salgado River, Ceará state, Brazil.</p><h3>Materials and Methods</h3><p>The monogeneans were affixed onto slides using Gray and Wess’s medium for examination of their sclerotized structures. For analysis of internal organs, a single specimen was preserved in 5% formalin, stained with Gomori’s trichrome, and mounted in Gray and Wess’s medium.</p><h3>Results</h3><p><i>Anacanthorus silvoi</i> n. sp. is characterized by having a short broad tube MCO with a medial constriction (i.e., MCO with distal region wider than the proximal region, and flexed lateral flap in the distal region in <i>A. cururutuiensis</i> and a MCO with a small projection in the form of a hook in the distal region in <i>A. siphonocommus</i>).</p><h3>Conclusions</h3><p>The present study corroborates previous studies that the absence of an accessory piece is a characteristic shared by all <i>Anacanthorus</i> members parasites of Erythrinidae.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1729 - 1735"},"PeriodicalIF":1.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Respiratory Syndrome Mimicking Shipping Sickness in Theileria buffeli Infected Buffalo Calf","authors":"Varghese Raina,&nbsp;Y. Ajith,&nbsp;Sasi Adithya,&nbsp;S. Anandu,&nbsp;N. Athira,&nbsp;K. S. Athira,&nbsp;P. Preena,&nbsp;K. Vinodkumar,&nbsp;K. Mathew Manju,&nbsp;C. S. Mahima,&nbsp;Jose Akshaya,&nbsp;B. Bruce Alby,&nbsp;Ayshin Nafis,&nbsp;A. Arshana,&nbsp;Joby Tini,&nbsp;P. Asok Anandu,&nbsp;Gopinath Devi,&nbsp;George Arun,&nbsp;S. Ajithkumar","doi":"10.1007/s11686-024-00932-w","DOIUrl":"10.1007/s11686-024-00932-w","url":null,"abstract":"<div><p>Benign theileriosis, caused by the members of the <i>Theileria orientalis</i> complex, can develop fatal clinical outbreaks characterized by acute respiratory manifestation in stressful conditions. This report describes the molecular diagnosis and clinical management of a recently transported buffalo calf with severe <i>Theileria buffeli</i> infection and associated acute pneumonia. A five-month-old male buffalo calf having an inter-state travel history three days back was presented with pyrexia, anorexia, weakness, mucoid rhinorrhoea, dyspnoea and diarrhoea from the day of procurement. The history and physical examination revealed a clinical presentation similar to shipping fever. Whereas, severe parasitemia of <i>Theileria</i> spp. with anaemia, thrombocytopenia and granulopenia were evident on laboratory investigation. The <i>Theileria</i> spp. infection was confirmed by PCR method using specific primers and the authentication was made by detailed sequence analysis. The small subunit rRNA was amplified using universal apicomplexan primers and the phylogenetic analysis was carried out for further characterisation. The animal was stabilized by steroid nebulization therapy and the specific chemotherapy was instigated using buparvaquone and sulphamethoxazole-trimethoprim combination. Supportive medications like non-steroidal anti-inflammatory drugs, antihistamines, antidiarrhoeals and vitamins were provided symptomatically. The animal showed a good response to therapy and recovered from parasitemia by day 10 and the molecular clearance was later confirmed on day 70 of therapy. The present case of <i>Theileria buffeli</i> infected buffalo calf with acute respiratory signs points towards the possible hemoparasitic outbreaks in transport-stressed animals with the signs of shipping fever-associated syndrome.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2073 - 2082"},"PeriodicalIF":1.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Gallbladder Ceratomyxidae (Myxozoa: Bivalvulida) Parasite Described In Pimelodella cristata (Müller & Troschel, 1848) From the Eastern Amazon","authors":"Abthyllane Amaral de Carvalho,&nbsp;Roger Leomar da Silva Ferreira,&nbsp;Lilia Suzane de Oliveira Nascimento,&nbsp;Saturo Cardoso Morais,&nbsp;Rafaela Franco de Araujo,&nbsp;Maylo de Almeida Costa,&nbsp;Marcela Nunes Videira,&nbsp;Elane Guerreiro Giese","doi":"10.1007/s11686-024-00930-y","DOIUrl":"10.1007/s11686-024-00930-y","url":null,"abstract":"<div><h3>Purpose</h3><p>The Myxozoa class is characterized by parasites that have valves joined by a suture line and polar capsules containing eversible spiral filamento and this class is considered an obligate parasite. The genus <i>Ceratomyxa</i> has approximately 300 species described in fish, both marine and freshwater fish, mainly infecting the gallbladder, but also occurring in the urinary bladder. This study describes a new species of <i>Ceratomyxa</i> in the Amazon region for <i>Pimelodella cristata</i>.</p><h3>Methods</h3><p>For these analyses, the fish were desensitized by means of a medullary section with the aid of a sharp metallic instrument. With the fish desensitized, the entire body surface was examined under a binocular stereoscopic microscope. The gallbladder fragments were collected and fixed in Davidson for histological analyses and in ethanol for molecular analyses.</p><h3>Results</h3><p>This parasite was found in the host’s gallbladder, with elongated spores in a decreasing shape in sutural view, measuring 1.64 ± 0.6 μm in length and 17.13 ± 2.6 μm in width. The polar capsules had a spherical shape of equal size and measured 1.36 ± 0.17 μm in length and 0.9 ± 0.05 μm in width, and each polar capsule contained 4 to 5 turns.</p><h3>Conclusion</h3><p>Morphological and phylogenetic analyzes denote that this is a new species of the genus <i>Ceratomyxa</i>.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2006 - 2015"},"PeriodicalIF":1.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Multiplex PCR Assay Reveals the Occurrence of E. bangladeshi alongside E. histolytica and E. moshkovskii in Eastern India","authors":"Sanjib Kumar Sardar,&nbsp;Sweety Mal,&nbsp;Ajanta Ghosal,&nbsp;Tapas Haldar,&nbsp;Akash Prasad,&nbsp;Chayanika Roy,&nbsp;Arjun Ghosh,&nbsp;Yumiko Saito-Nakano,&nbsp;Seiki Kobayashi,&nbsp;Shanta Dutta,&nbsp;Tomoysohi Nozaki,&nbsp;Sandipan Ganguly","doi":"10.1007/s11686-024-00921-z","DOIUrl":"10.1007/s11686-024-00921-z","url":null,"abstract":"<div><h3>Purpose</h3><p>Epidemiological studies on amoebic infections are complicated due to morphologically identical and clinically important <i>Entamoeba</i> species. Therefore, newer, simpler, and more economical diagnostic techniques are required for differentiating clinically important <i>Entamoeba</i> species.</p><h3>Methods</h3><p>We developed a single-round multiplex PCR assay to identify <i>E. histolytica</i>,<i> E. moshkovskii</i>,<i> E. dispar</i>,<i> E. bangladeshi</i>, and <i>E. coli</i>. Primers were designed based on variations in 18 S rRNA sequences. Sensitivity and specificity were assessed using known positive and negative samples. Furthermore, we screened 472 diarrheal samples using this technique alongside the reference PCR method to evaluate its suitability for epidemiological studies and clinical diagnosis. DNA sequencing and phylogenetic analysis of the isolates were conducted. All statistical analyses of the data were performed using GraphPad Prism.</p><h3>Results</h3><p>The designed primers successfully yielded species-specific PCR products of different sizes as expected. We did not observe any non-specific amplifications of the primer set. The diagnostic performance was also convincing. After screening clinical samples using the method, we observed that 2.33% (<i>n</i> = 11) tested positive for <i>E. moshkovskii</i>, 1.06% (<i>n</i> = 5) tested positive for <i>E. histolytica</i>, and 0.85% (<i>n</i> = 4) tested positive for <i>E. bangladeshi</i> in the studied area. DNA sequencing further confirmed the identified species. The constructed phylogenetic tree also demonstrated clear separation of the detected species lineages.</p><h3>Conclusion</h3><p>The study suggests the multiplex PCR assay could be a reliable diagnostic tool for amoebic infections. This study is particularly significant as it marks the first reported occurrence of <i>E. bangladeshi</i> since its documentation in South Africa and its native Bangladesh.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1886 - 1895"},"PeriodicalIF":1.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}