Acta Parasitologica最新文献

{"title":"The Effect of Curcumin on Chronic Toxoplasma gondii Infection in the Testes of BALB/c Mice","authors":"Mehdi Tavalla,&nbsp;Mohamad Sabaghan,&nbsp;Mojtaba Haghi Karamollah,&nbsp;Marzieh Pashmforosh,&nbsp;Ali Veisi,&nbsp;Fatemeh Mohtaseb,&nbsp;Shahrzad Mohtaseb,&nbsp;Seyed Ahmad Hosseini,&nbsp;Ghodratollah Shakerinejad,&nbsp;Ashraf Alemi,&nbsp;Mojtaba Kalantar","doi":"10.1007/s11686-026-01329-7","DOIUrl":"10.1007/s11686-026-01329-7","url":null,"abstract":"<div><h3>Purpose</h3><p>Toxoplasmosis is a widespread parasitic infection; it affects about 30% of the global population, either through acute toxoplasmosis or its sequels. Our aim was to determine how curcumin affected testicular infection in mice with chronic toxoplasmosis using <i>toxoplasma gondii strain ME49.</i></p><h3>Methods</h3><p>Forty male BALB/c mice (6–8 weeks old) weighing between 20 and 25 g were randomly divided into four groups. The control group, uninfected animals, received 1 cc of normal saline (vehicle) for 2 weeks. <i>Toxo</i> infection in the <i>Toxo</i> and <i>Toxo</i> + CUR groups continued for 4 weeks. After infection, animals in the <i>Toxo</i> and <i>Toxo</i> + CUR groups were treated orally for 2 weeks with 1 cc of normal saline (vehicle) or curcumin (CUR) (200 mg/kg) respectively [1]. Levels of oxidative stress markers, antioxidant enzyme activities and gene expression, sperm parameters, and histopathological changes were measured and evaluated [1]. Levels of oxidative stress indicators, antioxidant enzyme activity and gene expression, sperm parameters and histopathological changes were measured and evaluated.</p><h3>Results</h3><p><i>Toxoplasma gondii</i> infection decreased the activity and gene expression of testosterone and serum antioxidant enzymes (SOD, GPx, and CAT), while elevating FSH and LH levels. Histological alterations, including maturational anomalies, intratubular necrosis, and inflammatory infiltration, were noted in mice infected with <i>Toxoplasma gondii</i>. Curcumin decreased FSH and LH levels while enhancing sperm parameters, histological alterations, and the activity and gene expression of antioxidant enzymes (SOD, GPx, and CAT), as well as testosterone levels.</p><h3>Conclusion</h3><p>Curcumin treatment mitigated testicular infection induced by <i>Toxoplasma gondii</i> by enhancing antioxidant enzymes, improving sperm parameters, and decreasing pathological alterations in testicular tissue.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Phylogenetic and histopathological characterization of putative Hepatozoon ophisauri-like lineages in rodents from Italy","authors":"Davide Sogliani,&nbsp;Maria Sampieri,&nbsp;Filippo Maria Dini,&nbsp;Mattia Calzolari,&nbsp;Deborah Torri,&nbsp;Valentina Carta,&nbsp;Patrizia Bassi,&nbsp;Alicia Rojas,&nbsp;Giulia Maioli","doi":"10.1007/s11686-026-01328-8","DOIUrl":"10.1007/s11686-026-01328-8","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-026-01328-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Giardia Activity of Pistacia atlantica Hydroalcoholic Extract Against Giardia lamblia Cysts","authors":"Somayeh Tam,&nbsp;Delsuz Rezaee,&nbsp;Jahangir Abdi,&nbsp;Naser Abbasi,&nbsp;Nahid Maspi,&nbsp;Asad Mirzaei,&nbsp;Razi Naserifar,&nbsp;Elahe Karimi,&nbsp;Omid Raiesi,&nbsp;Hossein Seidkhani,&nbsp;Jalil Feizi","doi":"10.1007/s11686-026-01311-3","DOIUrl":"10.1007/s11686-026-01311-3","url":null,"abstract":"<div><h3>Background</h3><p>Giardiasis is a globally common protozoan disease with significant public health impact, causing diarrhea, nausea, weight loss, bloating, and abdominal pain. Current treatments face limitations including drug resistance and adverse effects, highlighting the need for alternative therapeutic agents. This study evaluated the cysticidal effects of hydroalcoholic extracts from the fruits and leaves of <i>Pistacia atlantica</i> subsp. <i>Kurdica</i> against <i>Giardia lamblia</i> cysts in vitro.</p><h3>Methods</h3><p><i>G. lamblia</i> cysts (1 × 10⁴/mL) were exposed to five concentrations of leaf and fruit extracts (0.25–4 mg/mL) for 12, 24, and 48 h at 37 °C. Saline buffer was the negative control and metronidazole (0.25–4 μg/mL) the positive control. Cyst viability was assessed by 0.1% eosin exclusion staining. All assays were performed in triplicate.</p><h3>Results</h3><p>The leaf extract demonstrated significant concentration- and time-dependent cysticidal activity at all time points (<i>P</i> &lt; 0.05), reaching 70–80% cyst mortality at 2–4 mg/mL after 48 h. The 0.25 mg/mL concentration showed no significant effect at 12 h (<i>P</i> &gt; 0.05). The fruit extract showed no cysticidal activity at any concentration or time point (<i>P</i> &gt; 0.05). Metronidazole was potent at all concentrations and time points (<i>P</i> &lt; 0.0001), with the leaf extract showing comparable efficacy (<i>P</i> &gt; 0.05).</p><h3>Conclusion</h3><p>The leaf extract of <i>P. atlantica</i> exhibited significant in vitro cysticidal activity against <i>G. lamblia</i> in a concentration- and time-dependent manner, comparable to metronidazole, while the fruit extract showed no activity. Further studies on trophozoite stages, phytochemical characterization, cytotoxicity, and animal models are needed before therapeutic conclusions can be drawn.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Gynandromorphism in Rhipicephalus microplus (Acari: Ixodidae) in El Salvador","authors":"Marvin Stanley Rodriguez Aquino,&nbsp;Esther Noemí Juárez Alvarado,&nbsp;Francisco Javier Castaneda Lúe,&nbsp;Pamela Michelle Cornejo Rivas,&nbsp;Melissa S. Nolan,&nbsp;Lídia Gual-Gonzalez","doi":"10.1007/s11686-026-01327-9","DOIUrl":"10.1007/s11686-026-01327-9","url":null,"abstract":"<div><h3>Purpose</h3><p>Gynandromorphism is a developmental anomaly characterized by the simultaneous presence of male and female sexual morphological traits within the same individual. This study documents a case of gynandromorphism in the cattle tick <i>Rhipicephalus microplus</i>(Canestrini 1888) in El Salvador.</p><h3>Methods</h3><p>The specimen was collected from a bovine belonging to the Experimental and Practical Station of the Faculty of Agronomic Sciences of the University of El Salvador, located in the municipality of San Luis Talpa, Department of La Paz.</p><h3>Results</h3><p>The reported gynandromorph of <i>R. microplus</i> exhibits bilateral gynandromorphism, displaying primarily female characteristics on the left side and male characteristics on the right.</p><h3>Conclusion</h3><p>This is the first report of a tick with gynandromorphism in El Salvador. Further studies are warranted to understand the implications of such anomalies and their veterinary and public health relevance.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-026-01327-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental effects on the lung and gastrointestinal parasite fauna of wild boar: a comparative study between alpine and Mediterranean ecosystems","authors":"Camilla Sangiovanni,&nbsp;Valerio Orazi,&nbsp;Irene Belardi,&nbsp;Leonardo Gallotta,&nbsp;Francesco Ferretti,&nbsp;Sonia Calderola,&nbsp;Patrizia Malaspina,&nbsp;Isabel Guadano-Procesi,&nbsp;Carlotta Fiorilla,&nbsp;David Di Cave,&nbsp;Federica Berrilli","doi":"10.1007/s11686-026-01309-x","DOIUrl":"10.1007/s11686-026-01309-x","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aims to explore environmental factors associated with parasite infections in faecal samples collected from wild boar populations, by analysing the prevalence, intensity, and abundance of parasite communities across two Italian ecosystems: the alpine environment in the Orco Valley (Gran Paradiso National Park) and the Mediterranean lowland in the Maremma Regional Park.</p><h3>Methods</h3><p>Seasonal faecal samples were collected from November 2023 to July 2025 along systematic transects in both study areas. Samples were analysed using the Mini-FLOTAC technique to quantify parasite eggs and (oo)cysts, and prevalence, intensity, and abundance were calculated. Environmental variables, including elevation, land use, temperature, precipitation, and season, were linked to each sample. Generalized linear models (GLMs) were fitted to evaluate the effects of environmental factors on parasite presence and abundance.</p><h3>Results</h3><p>Parasite communities were dominated by <i>Eimeria</i> spp., gastrointestinal strongyles, and <i>Metastrongylus</i> spp., whereas other taxa (e.g., <i>Balantioides coli</i>, <i>Capillaria</i> spp., <i>Cystoisospora</i> sp.) were sporadic. Significant differences in intensity and/or abundance of dominant taxa were observed between the two areas. Models based on infection abundance, rather than presence, revealed environmental associations: <i>Metastrongylus</i> spp. abundance decreased with increasing elevation, possibly reflecting constraints on intermediate host availability; while gastrointestinal strongyles abundance was lower in open natural habitats, reflecting microclimatic influences on larval survival.</p><h3>Conclusions</h3><p>This study highlights associations between habitat characteristics and parasite community structure in wild boar populations, providing insights into the environmental factors potentially influencing parasite transmission across heterogeneous landscapes.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-026-01309-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification of Cytochrome b Mutations in Buparvaquone-Unresponsive Theileria annulata Isolates from Khorasan-Razavi, Iran","authors":"Emad Changizi,&nbsp;Amin Esmaeilian,&nbsp;Hesam Ashareyoun,&nbsp;Gholamreza Razmi,&nbsp;Hamid Staji,&nbsp;Iraj Ashrafi Tamai","doi":"10.1007/s11686-026-01317-x","DOIUrl":"10.1007/s11686-026-01317-x","url":null,"abstract":"<div><h3>Introduction</h3><p>The failure of conventional therapies for bovine theileriosis in Khorasan-Razavi province and other regions of Iran has led to significant economic losses and raised concerns about rural livestock sustainability and national food security. This study identified specific cytochrome b mutations in <i>Theileria annulata</i> isolates collected from cattle showing clinical unresponsiveness to buparvaquone treatment in the Khorasan-Razavi province of Iran.</p><h3>Materials and Methods</h3><p>Blood samples were collected, and parasite DNA was extracted and amplified via PCR using specific primers. </p><h3>Results</h3><p>Sequencing and subsequent BLAST analysis revealed two novel cytochrome b variants, OQ230576 and OQ230577, registered in the NCBI GenBank. These variants exhibited multiple non-synonymous mutations compared to sensitive reference strains from Iran (MH248139/1) and Ankara. The OQ230576 sequence demonstrated six to ten mutations, while OQ230577 exhibited eight to ten mutations, suggesting structural alterations linked to drug resistance.</p><h3>Conclusions</h3><p>These findings confirm the emergence and spread of resistant Theileria annulata strains in the studied regions. The progressive decline in treatment efficacy underscores the urgent need for alternative therapeutic approaches and more robust disease management strategies to safeguard livestock productivity and rural economies.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection of Mermithid Nematodes in a Natural Population of Armadillidium vulgare in Japan","authors":"Anzai Naoya,&nbsp;Tsukasa Waki","doi":"10.1007/s11686-026-01323-z","DOIUrl":"10.1007/s11686-026-01323-z","url":null,"abstract":"<div><h3>Purpose</h3><p>Parasites are generally thought to regulate host population density by reducing reproductive activity and/or increasing host mortality. This study examined the infection of nematodes of the family Mermithidae, which may have lethal and castrating effects on their hosts, in a natural population of the host isopod <i>Armadillidium vulgare</i> in Japan.</p><h3>Methods</h3><p>Isopods were collected almost monthly (2021–2023) using quadrat sampling (<i>N</i> = 3, 50 cm × 50 cm) at a station in Japan. We recorded prevalence of hosts, numbers of sampled hosts and body length frequency distributions of infected and uninfected hosts.</p><h3>Results</h3><p>Among 1553 hosts sampled, the overall infection prevalence was 4.2%. Particularly high values between 10% and 28% were observed from June to July, which is the period of the hosts’ reproduction and subsequent natural death.</p><h3>Conclusion</h3><p>While the lethal effects of infection may have a limited impact on host density, infection during the reproductive season suggests decrease in reproductive activity, potentially reducing the population size of the next host generation.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomological Surveillance and Arbovirus Screening in Mosquitoes from Central Morocco: First Detection of West Nile Virus in Culex pipiens from the Marrakech-Safi Region","authors":"Abdelkrim Outammassine,&nbsp;Mattia Calzolari,&nbsp;Mohamed Daoudi,&nbsp;Paolo Bonilauri,&nbsp;Annalisa Grisendi,&nbsp;Samia Boussaa,&nbsp;Michele Dottori,&nbsp;Said Zouhair,&nbsp;Souad Loqman","doi":"10.1007/s11686-026-01320-2","DOIUrl":"10.1007/s11686-026-01320-2","url":null,"abstract":"<div><h3>Purpose</h3><p>Surveillance of arboviral vectors and screening for probable infection is very important for the planning of vector control programs, especially in countries where these activities are just beginning. In the present study, we conducted mosquito surveillance at 26 locations predicted to be at risk (according to our recently published study using Machine learning) in the Marrakech-Safi region of central Morocco.</p><h3>Methods</h3><p>The field studies were conducted for six consecutive months, from April to October, in 2019 and 2021. Two different methods were used to collect adult mosquitoes, human landing catches (by mouth aspirator), and CDC miniature light traps; larvae and pupae were collected using dippers.</p><h3>Results</h3><p>A total of 1907 mosquitoes were collected, belonging to 15 species across five genera (<i>Aedes</i>, <i>Culex</i>, <i>Culiseta</i>, <i>Anopheles</i>, and <i>Coquillettidia</i>)<i>.</i> Out of the 1907 mosquitoes that were sampled, 53.7% (1025/1907) were in the larval stage, 42.8% (818/1907) were adults, and the remaining 3.3% (64/1907) were pupae. Among the collected and identified adults, the females represented 96.6% and blood-fed females accounted for 55.9%. The adult mosquitoes were screened for five arboviruses: dengue virus (DENV), chikungunya virus (CHIKV), Zika virus (ZIKV), Rift Valley fever virus (RVFV), and West Nile virus (WNV) by using RT-qPCR, and a pool of <i>Cx. pipiens</i> from Tabia (Station 14) was positive for WNV.</p><h3>Conclusion</h3><p>This is the first detection of WNV in <i>Cx. pipiens</i> or any other mosquito species from central Morocco. The detection of several vectors within the region suggests a risk for arboviral transmission in central Morocco and appeals for entomological vigilance and the urgent need to undertake and enhance periodic surveillance campaigns in the region.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148222457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and phylogenetic characterization of Babesia and Ehrlichia in free-living opossums from southeastern Brazil","authors":"Stephany Rocha Ribeiro,&nbsp;Larissa Pereira Rodrigues,&nbsp;João Otávio Mochiuti,&nbsp;Catharina Eulasya Viana Resende,&nbsp;Gilberto Salles Gazeta,&nbsp;Ana Beatriz Borsoi,&nbsp;Mariana Guimarães Côrtes da Cruz,&nbsp;Karla Bitencourth,&nbsp;Rodrigo Hidalgo Friciello Teixeira,&nbsp;Lígia Souza Silveira da Lima","doi":"10.1007/s11686-026-01325-x","DOIUrl":"10.1007/s11686-026-01325-x","url":null,"abstract":"<div><h3>Purpose</h3><p>This study investigated the occurrence of vector-borne pathogens in free-living opossums (<i>Didelphis albiventris</i> and <i>Didelphis aurita</i>) from São Paulo State, Brazil, with emphasis on <i>Babesia</i> spp., <i>Hepatozoon</i> spp., and <i>Ehrlichia</i> spp.</p><h3>Methods</h3><p>Blood samples from 115 opossums (95 <i>D. albiventris</i> and 20 <i>D. aurita</i>) were analyzed using parasitological and molecular approaches. Conventional PCR assays were performed for <i>Babesia</i> spp. and <i>Hepatozoon</i> spp., whereas nested PCR was used for <i>Ehrlichia</i> spp. Positive amplicons were sequenced and subjected to phylogenetic analysis.</p><h3>Results</h3><p>Five samples (4.35%) from <i>D. albiventris</i> collected in Botucatu were positive for <i>Babesia</i> spp. based on the 18 S rRNA gene, and two samples (1.74%) were positive for <i>Ehrlichia</i> spp. based on the <i>dsb</i> gene. No <i>Hepatozoon</i> spp. DNA was detected. Sequence analysis revealed 100% identity between the <i>Babesia</i> amplicons and <i>Babesia</i> sp. (GenBank: MW290046), while one <i>Ehrlichia</i> sequence showed 100% identity with <i>Ehrlichia</i> sp. strain Natal (GenBank: KY207546; OP270482), and the second sequence showed 99.71% identity with <i>Ehrlichia</i> sp. (GenBank: OP270482). Phylogenetic reconstruction clustered the <i>Babesia</i> sequences within the South American Marsupialia Group and positioned the <i>Ehrlichia</i> sequences together with lineages previously reported in South American marsupials.</p><h3>Conclusion</h3><p>These findings provide the first molecular detection of <i>Babesia</i> sp. and <i>Ehrlichia</i> sp. in free-living <i>D. albiventris</i> from Botucatu, São Paulo State, Brazil. The results expand current knowledge on vector-borne pathogens in synanthropic marsupials and reinforce the importance of continued surveillance to improve understanding of host–pathogen interactions at urban–wildlife interfaces within a One Health perspective.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-026-01325-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148215876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytochrome P450 Monooxygenase Mediated Pyrethroid Resistance in the Urban Dengue Vector, Aedes aegypti (Diptera: Culicidae) from Northern Part of West Bengal, India","authors":"Prapti Das,&nbsp;Subhajit Das,&nbsp;Abhirup Saha,&nbsp;Abhinna Tamang,&nbsp;Dhiraj Saha","doi":"10.1007/s11686-026-01319-9","DOIUrl":"10.1007/s11686-026-01319-9","url":null,"abstract":"<div><h3>Purpose</h3><p>Development of pyrethroid resistance in <i>Aedes aegypti</i> (Diptera: Culicidae) can lead to failure of ongoing vector control strategies and thereby, impede prevention of dengue transmission in urban regions of India. Therefore, its resistance status needs to be properly understood to prevent recurring dengue outbreaks in cities. The present study investigated cytochrome P450 monooxygenase (CYP)-mediated pyrethroid resistance in this vector species from dengue endemic northern part of West Bengal, India.</p><h3>Methods</h3><p>Larvae and pupae of wild <i>Ae. aegypti</i> populations were collected from urban regions of two districts (Siliguri town from Darjeeling and Raiganj from Uttar Dinajpur). Larval and adult bioassays were performed for two synthetic pyrethroids viz. deltamethrin and bifenthrin. Synergist assay with Piperonyl Butoxide (PBO), a CYP blocker, was conducted to understand the involvement of CYPs. Relative expression of four CYP9 family genes: <i>CYP9J24</i>, <i>CYP9J26</i>, <i>CYP9J28</i> and <i>CYP9J32</i> was also studied through Quantitative Reverse Transcription PCR (qRT-PCR) in larvae and adults.</p><h3>Results</h3><p>Larvae and adults of wild <i>Ae. aegypti</i> populations were found resistant for both pyrethroids. Pre-exposure to PBO restored susceptibility in both larvae and adults, indicates the involvement of CYPs in the observed resistance. Differential over-expression of the CYP9 family genes in larvae and adults strengthened association of CYPs with pyrethroid resistance of studied populations.</p><h3>Conclusion</h3><p>A control strategy targeting CYPs can be effective in management of <i>Ae. aegypti</i> in this region. Moreover, use of synergists like CYP blockers can help in restoration of susceptibility to pyrethroids in wild <i>Ae. aegypti</i> populations of northern part of West Bengal.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148196981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}