Acta Parasitologica最新文献

{"title":"Epidemiological Study on Humans, Animals and Snails for Schistosomiasis in Two Endemic Municipalities Nearing Elimination in Bohol, the Philippines","authors":"Jose Ma. M. Angeles,&nbsp;Katrina Theresa M. Balboa,&nbsp;Frances Paula L. Miaral,&nbsp;Klyde Irene M. Ligot,&nbsp;Kevin Austin L. Ona,&nbsp;Maria Luz B. Belleza,&nbsp;Elena A. Villacorte,&nbsp;Pilarita T. Rivera,&nbsp;Shin-ichiro Kawazu,&nbsp;Aya Yajima","doi":"10.1007/s11686-024-00973-1","DOIUrl":"10.1007/s11686-024-00973-1","url":null,"abstract":"<div><h3>Purpose</h3><p>A comprehensive survey was conducted to assess the prevalence of <i>Schistosoma japonicum</i> infection in humans, water buffaloes, and snails in the two endemic municipalities of Talibon and Trinidad in Bohol, Philippines, which are nearing elimination.</p><h3>Methods and Results</h3><p>Human stool and blood samples were collected from barangays with snail breeding sites, and results showed higher positivity rates using the rSjTPx-1-ELISA compared to the Kato-Katz technique. Human stool examination for showed a 0.57% positivity rate for <i>S. japonicum</i> in San Vicente, Trinidad, while no cases were found in San Roque, Talibon. On the other hand, 3.43% of human serum samples from San Vicente, Trinidad, and 46.20% from San Roque, Talibon tested seropositive. Similarly, water buffalo samples revealed 16.95% seropositivity in Talibon and 10% in Trinidad. Additionally, snail infection was determined microscopically from collected <i>Oncomelania hupensis quadrasi</i> snails in Talibon, but no schistosome parasites were detected in the crushed snail tissues.</p><h3>Conclusion</h3><p>These findings highlight that despite progress towards elimination, schistosomiasis transmission persists in these areas, emphasizing the need for continued surveillance and targeted interventions.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Intestinal Parasitic Infections and Associated Factors Among Food Handlers in East Africa: A Systematic Review and Meta-analysis","authors":"Agenagnew Ashagre,&nbsp;Tadesse Misganaw,&nbsp;Wagaw Abebe,&nbsp;Zelalem Dejazmach,&nbsp;Gashaw Azanaw Amare,&nbsp;Yenesew Mihret Wondmagegn,&nbsp;Kassahun Misgana Worku,&nbsp;Adane Adugna,&nbsp;Hassen Ahmed,&nbsp;Solomon Gedifie,&nbsp;Getinet Kumie,&nbsp;Marye Nigatie,&nbsp;Abdu Jemal,&nbsp;Woldeteklehaimanot Kasahun,&nbsp;Sisay Ayana,&nbsp;Zelalem Asmare,&nbsp;Yalewayker Gashaw,&nbsp;Ermias Getachew,&nbsp;Muluken Gashaw,&nbsp;Assefa Sisay,&nbsp;Selamyhun Tadesse,&nbsp;Biruk Beletew Abate,&nbsp;Atitegeb Abera Kidie,&nbsp;Melesse Abate Reta","doi":"10.1007/s11686-024-00968-y","DOIUrl":"10.1007/s11686-024-00968-y","url":null,"abstract":"<div><h3>Background</h3><p>Intestinal parasitic infections are a significant public health concern, especially among food handlers, who can transmit these infections to the public through food preparation and handling. This systematic review and meta-analysis aimed to determine the pooled prevalence and associated factors of intestinal parasitic infections among food handlers in the East African region.</p><h3>Methods</h3><p>A systematic review and meta-analysis on intestinal parasitic infections among food handlers involved a comprehensive search across various databases, including Scopus, PubMed, ScienceDirect, Google Scholar, and the institution’s library registers. Forty relevant articles were identified and analyzed using STATA Software version 17.0. Sensitivity analysis, publication bias assessment with Egger’s test, and the Trim-and-fill meta-analysis for bias adjustment were conducted. Heterogeneity across the studies was assessed using Cochran’s Q statistic and I² statistics, and subgroup analysis computed for significant heterogeneity (I² value ≥ 50%). A random effect model was used to determine the pooled prevalence of intestinal parasitic infections.</p><h3>Results</h3><p>The pooled prevalence of intestinal parasitic infections among food handlers was 32.27% (95% CI 27.90–36.65). The most prevalent parasites were <i>Entamoeba histolytica/dispar</i> 20.83% (95% CI 13.66–28%), <i>Ascaris lumbricoides</i> 13.84% (95% CI 10–17.68%), <i>Giardia lamblia</i> 8.55% (95% CI 6.03–11.06%), and hookworm 6.43% (95% CI 3.93–8.93%). Using a common knife for cutting raw meat (AOR = 2.27, 95% CI 1.21–4.31), food handler’s untrimmed fingernails (AOR = 2.14, 95% CI 1.50–2.78), and no hand washing practices with soap after using the toilet (AOR = 2.25, 95% CI 1.33–3.18) were associated with higher rates of intestinal parasitic infections among food handlers.</p><h3>Conclusions</h3><p>Parasitic infections among food handlers were found to be significantly prevalent. Factors contributing to this high prevalence included food handlers’ untrimmed fingernails, poor hand hygiene practices, and using a shared knife for chopping various food items, including raw meat. These findings emphasize the need for proper personal hygiene and sanitation practices among food handlers to prevent transmitting parasitic infections to consumers.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cinnamaldehyde and Its Nanoemulsion on Acanthamoeba castellanii Cysts and Trophozoites","authors":"Somayeh Bahrami,&nbsp;Fatemeh Navidi,&nbsp;Maryam Ghaderi-Ghahfarokhi,&nbsp;Mehdi Zarei","doi":"10.1007/s11686-024-00938-4","DOIUrl":"10.1007/s11686-024-00938-4","url":null,"abstract":"<div><h3>Purpose</h3><p>The current study aimed to investigate the trophocidal and cysticidal activities of cinnamaldehyde (Cinn), a natural compound with known antimicrobial properties, against environmental and reference strains of <i>Acanthamoeba castellanii</i>. Additionally, we explored the potential benefits of Cinn formulated as a nanoemulsion (Cinn-NE) in enhancing its efficacy.</p><h3>Methods</h3><p>Cinn-NE was prepared using the ultrasonic emulsification method. The amoebicidal effect of Cinn was evaluated against both trophozoites and cysts of reference and environmental strains of Acanthamoeba, along with an assessment of whether nanotechnology enhances this effect.</p><h3>Results</h3><p>Our results demonstrated that Cinn exhibited strong activity against both trophozoites and cysts of <i>Acanthamoeba</i>. Importantly, Cinn-NE showed enhanced activity compared to pure Cinn, possibly due to increased surface area and improved interaction with microbial membranes. In this study, the difference in susceptibility to Cinn and Cinn-NE between the environmental and the reference strain of <i>A. castellanii</i> was observed. <i>Acanthamoeba</i> cysts were more resistant to the effects of Cinn or Cinn-NE than trophozoites. Additionally, we found that nutrient availability in the medium influenced the susceptibility of <i>Acanthamoeba</i> to Cinn and Cinn-NE. A nutrient-deficient medium reduced their vulnerability to destruction, suggesting a role for nutrient availability in the response of <i>Acanthamoeba</i> to antimicrobial agents.</p><h3>Conclusion</h3><p>In conclusion, our study highlights the potential of Cinn and Cinn-NE as effective agents for combating <i>Acanthamoeba</i> infections. Further research is needed to elucidate the specific mechanisms underlying the differential susceptibility to Cinn, optimize its use as a treatment or surface disinfectant, and explore Acanthamoeba's cellular and molecular responses to nutrient availability.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of a Conventional Assay into SYBR Green Real-Time PCR for the Detection of the Nc5 Segment from Neospora caninum","authors":"Flávia Moreira da Fonseca,&nbsp;Marilia de Oliveira Koch,&nbsp;Ana Paula Sato,&nbsp;Maria Constanza Rodriguez,&nbsp;Rosangela Locatelli-Dittrich","doi":"10.1007/s11686-024-00939-3","DOIUrl":"10.1007/s11686-024-00939-3","url":null,"abstract":"<div><h3>Purpose</h3><p>The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the <i>Neospora caninum</i> genome.</p><h3>Methods</h3><p>The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations. The NP7/NP10 assay was selected and reaction conditions optimized. Efficiency, analytical sensitivity, precision and specificity were assessed. The assay was evaluated in triplicate, in three independent PCR runs performed by two technicians to generate robust results.</p><h3>Results</h3><p>The standard curve determined by tenfold serial dilutions (1 to 1 × 10–⁷) established a reaction efficiency (E) of 102.34%, a correlation coefficient (R²) of 0.999 and a slope of -3.267. LOD of the real-time PCR assay was 0.456 tachyzoites DNA per reaction, as compared to 45.62 for the conventional method. SYBR green real-time PCR was 100 times more sensitive than the conventional method. Precision analysis showed 100% intra- and inter-assay repeatability at the minimum detection limit. The mean assay coefficient of variation (CV%) was 4.19% and standard deviation (SD) 1.67%. No significant differences between the means of <i>Cq</i> in the replicates and technicians (P &gt; 0.05) was found, indicating that the assay is robust and accurate. The applicability of the assay was tested and <i>N. caninum</i> DNA was detected in milk, blood, amniotic fluid, placenta and different tissue samples.</p><h3>Conclusion</h3><p>The protocol had high specificity, confirmed by melting curve analysis and no cross-reactions with other tested microorganisms. The SYBR Green-based PCR protocol standardized in this study is a highly sensitive and specific method, reproducible and applicable for the detection of <i>N. caninum</i> in different biological samples.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flies as Carriers of Gastrointestinal Protozoa of Interest in Public Health in the Northeast of Brazil","authors":"João Victor Batista dos Santos,&nbsp;Anna Luiza Hora dos Santos,&nbsp;André Mota Alves,&nbsp;Matheus Resende de Oliveira,&nbsp;Kalina Maria de Medeiros Gomes Simplicio,&nbsp;Lorena Maciel Santos Silva,&nbsp;João Victor de Jesus,&nbsp;Michelle Evangelista Soares,&nbsp;Ana Cinthia Santos da Costa,&nbsp;Weslania Sousa Inacio da Silva,&nbsp;Cláudia Moura de Melo,&nbsp;Rubens Riscala Madi,&nbsp;Victor Fernando Santana Lima","doi":"10.1007/s11686-024-00935-7","DOIUrl":"10.1007/s11686-024-00935-7","url":null,"abstract":"<div><h3>Purpouse</h3><p>This study aimed to assess the influence of the presence of synanthropic flies in food preparation environments on the transmission of potentially zoonotic gastrointestinal protozoa.</p><h3>Methods</h3><p>Flies were captured using a glass containing water, fruits, and pieces of protein.</p><h3>Results</h3><p>Approximately 260 flies from four different species were captured: <i>Musca domestica</i> (76.92%; 200/260), <i>Cochliomyia hominivorax</i> (17.31%; 45/260), <i>Lucilia cuprina</i> (3.85%; 10/260), and <i>Hydrotaea aenescens</i> (1.92%; 5/260). Protozoa were identified through microscopic analysis after macerating these arthropods contained in their respective eppendorfs, using two techniques, namely Mini-FLOTAC^® and centrifugal sedimentation with Ziehl–Neelsen staining. The analysis revealed a concerning scenario regarding the epidemiological chain of gastrointestinal protozoa in Sergipe, Northeast Brazil. The positivity rate was 26.92% (14/52) of the pools, with 100.00% (5/5) in Aracaju, 30.00% (3/10) in Nossa Senhora do Socorro, 27.27% (3/11) in Nossa Senhora da Glória, 12.50% (2/16) in Carmópolis, and 10.00% (1/10) in Nossa Senhora das Dores. Two species of protozoa were identified, namely <i>Cryptosporidium</i> spp. (23.08%; 12/52) and <i>Entamoeba</i> spp. (9.62%; 5/52). The involvement of two fly species in the maintenance of the life cycle of these protozoa was noticeable, specifically <i>M. domestica</i> (27.50%; 11/40) and <i>C. hominivorax</i> (22.22%; 2/9).</p><h3>Conclusions</h3><p> Counties analyzed had an human development index (HDI) considered medium; however, they still faced socioeconomic problems such as absence of sanitation systems, waste accumulation in the streets, and illegal waste disposal, which could favor the proliferation of these vectors and the spread of these gastrointestinal protozoa.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2055 - 2063"},"PeriodicalIF":1.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: The Protective Role of Toll-Like Receptor Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis","authors":"Ibrahim Aly,&nbsp;Essam H. Ibrahim,&nbsp;Rabab S. Hamad,&nbsp;Hoda E. L. Sayed,&nbsp;Sama M. N. Attiyah,&nbsp;Wafaa E-Komy,&nbsp;Hamed A. Ghramh,&nbsp;Ali Alshehri,&nbsp;Khalid M. Alsyaad,&nbsp;Mohammed Alshehri,&nbsp;Mona Kilany,&nbsp;Kareem Morsy,&nbsp;Attalla F. El-kott,&nbsp;Ramadan Taha","doi":"10.1007/s11686-024-00936-6","DOIUrl":"10.1007/s11686-024-00936-6","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2097 - 2097"},"PeriodicalIF":1.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Modern Morphological and Molecular Description of Saccocoelium Cephali Larvae Stages (Digenea: Haploporidae) from the Black Sea","authors":"Yuliya V. Belousova,&nbsp;Dmitry M. Atopkin","doi":"10.1007/s11686-024-00934-8","DOIUrl":"10.1007/s11686-024-00934-8","url":null,"abstract":"<div><h3>Purpose</h3><p>Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from the Black and Medditeranean Seas were known. The present research is the first report of haploporid cercariae <i>S. cephali</i> from the gastropod <i>Hydrobia acuta</i> inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from the Haploporidae family.</p><h3>Methods</h3><p><i>Hydrobia acuta</i> molluscs were collected in the estuary of the Chernaya River (the Black Sea) during 2011–2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species, and molecular comparisons were made of the subunit ribosomal DNA with other Haploporidae species.</p><h3>Results</h3><p>The haploporid parthenitae were found in the gonads of <i>Hydrobia acuta</i> molluscs. Most of the morphological characteristics of the specimens investigated agree with those of <i>Saccococoelium </i>sp<i>.</i> and are closest to those of <i>S. cephali</i> by shape and length of the body, ratio of suckers, ratio of tail length to body, shape, and size of the pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens from the Black Sea were within the hapolporine clade and closely related to <i>S. cephali</i> from Spain.</p><h3>Conclusion</h3><p>Morphological characteristics of cercariae emerging from <i>Hydrobia acuta</i> from the Black Sea and the analysis of partial 28S rDNA sequences support the conspecificity of the parasite with <i>S. cephali</i> mature worms from the Mediterranean Sea.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1919 - 1925"},"PeriodicalIF":1.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Liver and Jejunal Abscesses Due to Fasciola Flat Worm: An Uncommon Case Report from Iran","authors":"Shahriar Alian,&nbsp;Shadi Shayesteazar,&nbsp;Eissa Soleymani,&nbsp;Farzad Yazdani,&nbsp;Mohammad Reza Azimi,&nbsp;Mahdi Fakhar","doi":"10.1007/s11686-024-00931-x","DOIUrl":"10.1007/s11686-024-00931-x","url":null,"abstract":"<div><h3>Introduction</h3><p>Fascioliasis is a zoonotic condition precipitated by the trematode known as <i>Fasciola</i> spp. This parasite affected almost 2.4 to 17 million individuals in the world. The occurrence of liver abscesses is an infrequent phenomenon, potentially arising as a secondary outcome of cholangitis or hepatic inflammation. In this study, we have reported a case of a hepatic abscess induced by <i>Fasciola</i> spp.</p><h3>Case Report</h3><p>A 29-year-old male presented with generalized abdominal discomfort, primarily concentrated in the right upper quadrant, which had progressively worsened over the past seven months. The patient did mention a weight loss of approximately 10 kg during the course of their illness, along with increasing fatigue. Eosinophilia was seen too (35%). The stool exam test was negative. An enteroscopy was conducted, revealing no pathological abnormalities. Following this, the surgeon prescribed a surgical biopsy of the mass, which yielded a diagnosis of multiple eosinophilic granulomatous abscesses within the jejunal wall and mesentery. The <i>Fasciola</i> IgG test returned a positive result. After of follow-up, the patient fully recovered.</p><h3>Conclusion</h3><p>Fascioliasis should be considered in the differential diagnoses of liver abscesses. Because of earlier diagnosis, can be avoided of invasive diagnostic tests and therapeutic interventions can be started earlier.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2051 - 2054"},"PeriodicalIF":1.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Changes in the Tegument and Tissues of Fasciola hepatica Adults and Their Eggs Due to the Effect of an Ethyl Acetate Extract of Artemisia ludoviciana Nutt. spp Mexicana","authors":"Alonso Ezeta-Miranda,&nbsp;José Guillermo Avila-Acevedo,&nbsp;Adriana Montserrat Espinosa-González,&nbsp;José del Carmen Benítez-Flores,&nbsp;Gerardo Francisco-Marquez,&nbsp;Yolanda Vera-Montenegro","doi":"10.1007/s11686-024-00933-9","DOIUrl":"10.1007/s11686-024-00933-9","url":null,"abstract":"<div><h3>Purpose</h3><p>The objective of the present work was to evaluate the effect of an ethyl acetate extract of <i>Artemisia ludoviciana</i> on the viability of adult <i>Fasciola hepatica</i> parasites and eggs.</p><h3>Methods</h3><p>The collection of plant material was performed as described in previous reports. The dried material was macerated with ethyl acetate. Ovicidal assays were performed at concentrations of 100, 200, 300, 400 and 500 mg/L <i>A. ludoviciana</i> extract. Bioassays of fasciolicidal efficacy in adult specimens of <i>F. hepatica</i> were performed at extract concentrations of 125, 250, 375 and 500 mg/L. The effects of triclabendazole, a reference drug, and artemisinin were also evaluated.</p><h3>Results</h3><p>The ovicidal effectiveness of the extracts obtained after 16 h of incubation at concentrations of 100, 200, 300, 400 and 500 mg/L was 48%, 52%, 87%, 89% and 92%, respectively (<i>p</i> &lt; 0.05), and the fasciolicidal efficiencies during the first 24 h post-treatment ranged from 82 to 100% (<i>p</i> &lt; 0.05). In both cases, scanning electron microscopy revealed damage to the shells of the eggs treated with the extract, compromising their stability. In adult fasciolae, alterations to the integument that resulted in its erosion and detachment were observed. Histopathological studies of the affected specimens revealed damage to the tegumentary and subtegumentary cells and alterations in the ovaries, testicles and intestine. This damage was more severe after treatment with the extract than after treatment with the other compounds.</p><h3>Methods</h3><p>Extract of <i>A. ludoviciana</i> causes damage to the tegument, intestine, ovaries, testes and eggs of <i>F. hepatica</i>.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2016 - 2030"},"PeriodicalIF":1.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00933-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species of Heligmonellidae (Nematoda) in Euryoryzomys russatus (Rodentia: Sigmodontinae) in the Atlantic Forest of Northeast Brazil","authors":"Natália Alves Costa,&nbsp;Rosana Gentile,&nbsp;Martin R. Alvarez,&nbsp;Arnaldo Maldonado Junior","doi":"10.1007/s11686-024-00928-6","DOIUrl":"10.1007/s11686-024-00928-6","url":null,"abstract":"<div><h3>Background</h3><p>The genus <i>Stilestrongylus </i>(Freitas, Lent, and Almeida, 1937) is defined by having 24 or more subequal cuticular ridges, an asymmetrical caudal bursa, and a hypertrophied genital cone. It comprises 25 recognized species, mainly parasitizing cricetids, and shows significant diversification linked to the evolution of its hosts. <i>Stilestrongylus magnumspiculum </i>n. sp. is a newly described nematode species identified in the small intestine of the rodent <i>Euryoryzomys russatus</i> (Cricetidae, Sigmodontinae) from the Atlantic Forest of Northeast Brazil. This study aims to characterize the new species and its prevalence in host populations.</p><h3>Methods</h3><p>A total of 43 <i>Euryoryzomys russatus </i>individuals were collected and analyzed for parasitic infections. Morphological features of <i>Stilestrongylus magnumspiculum </i>n. sp. were examined, focusing on the number of ridges, synlophe characteristics, caudal bursa type, lobe hypertrophy, and the spicule length to body length ratio.</p><h3>Results</h3><p>The new species exhibited a prevalence of 21% among the sampled hosts. Distinctive morphological features were observed, including:the synlophe structure exhibited specific ridge patterns that differentiate it from other species within the genus; the caudal bursa was identified as a type 2-2-1; hypertrophy of the right lobe was observed, indicating notable morphological adaptation; rays 4 and 5 were found to be equivalent in size and diverged at the distal end; the spicule length to body length ratio (SpL/BL) was measured at 30–36%, representing the largest ratio recorded for the genus. In comparison, other <i>Stilestrongylus </i>species displayed the following ratios: <i>S. rolandoi </i>(21–33%), <i>S. lanfrediae</i> (25–29%), <i>S. kaaguyporai</i> (20–24%), <i>S. inexpectatus </i>(20–23%), and <i>S. stilesi </i>(26–28%).</p><h3>Conclusions</h3><p>This report presents <i>Stilestrongylus magnumspiculum </i>n. sp. as a new species of nematode, contributing to the diversity of the genus <i>Stilestrongylus</i>. The findings underscore the importance of studying host-parasite interactions within the Atlantic Forest ecosystem and provide a basis for future ecological and parasitological research.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1911 - 1918"},"PeriodicalIF":1.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00928-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}