Acta Parasitologica最新文献

{"title":"Detection of Toxoplasma gondii in Brain Tissues of Pet Birds with Neurological Symptoms Using Loop-Mediated Isothermal Amplification (LAMP) and PCR","authors":"Mohammad Neshatpour Esfahani,&nbsp;Majid Gholami-Ahangaran,&nbsp;William J. Sullivan Jr.","doi":"10.1007/s11686-024-00983-z","DOIUrl":"10.1007/s11686-024-00983-z","url":null,"abstract":"<div><h3>Background</h3><p>This study seeks to close this divide by assessing the occurrence of <i>Toxoplasma gondii</i> (<i>T. gondii</i>) in the brain tissues of pet birds displaying neurological symptoms, utilizing Nested Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplification (LAMP) methods. Furthermore, it aims to evaluate and contrast the sensitivity and specificity of different diagnostic procedures. Seventy bird samples, consisting of 50 sick and 20 asymptomatic samples, were assessed using various methods. Brain tissue DNA was isolated and then analyzed using nested PCR and LAMP assays targeting the <i>T. gondii</i> B1 gene and RE gene. Sensitivity and specificity assessments were conducted, and statistical evaluations were made using SPSS software.</p><h3>Results</h3><p>Our findings revealed that no <i>T. gondii</i> cysts were detected in the smear preparations. However, <i>T. gondii</i> DNA was found in 6% of the sick birds using nested PCR, and LAMP identified <i>T. gondii</i> DNA in 8% of these individuals. Both techniques demonstrated a high level of specificity, with LAMP showing higher sensitivity and faster processing times than nested PCR. These results significantly contribute to our understanding of toxoplasmosis and highlight the reliability and potential for the widespread use of these diagnostic techniques, providing reassurance and confidence in their effectiveness.</p><h3>Conclusion</h3><p>The study underscores the importance of molecular diagnostics in promptly identifying and treating toxoplasmosis in birds. This enhances our understanding and control of the disease and underscores the significant impact of this research. The potential for further studies using more extensive and diverse bird populations to enhance surveillance and prevention strategies against <i>T. gondii</i> infections is promising. It should be actively pursued, giving us hope for a better future in avian health.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Arum rupicola Boiss rupicola Extracts on Visceral Larva Migrans in Mice","authors":"Gozde Nur Akkus,&nbsp;Tugrul Atalay,&nbsp;Sinem Akdeniz,&nbsp;Husamettin Ekici,&nbsp;Dincer Yildiz,&nbsp;I. Safa Gurcan,&nbsp;Kader Yildiz","doi":"10.1007/s11686-024-00970-4","DOIUrl":"10.1007/s11686-024-00970-4","url":null,"abstract":"<div><h3>Purpose</h3><p>In the present study, the effects of leaf and rhizome extracts of <i>Arum rupicola</i> Boiss <i>rupicola</i> were searched on the infective stage <i>Toxocara canis</i> larvae (L3) in the experimentally infected mice.</p><h3>Methods</h3><p>Four-six week-old male BALB/c mice were divided into eight groups (G1-8, each group consisted of 7 mice), and they were infected orally with 500 <i>T. canis</i> eggs with L3. After 24 h, the following treatment protocol was applied to the groups for five days: G-1: Albendazole (ABZ) (100 mg/kg), G-2: Leaf Extract (10 mg/mL) + ABZ (100 mg/kg), G-3: Leaf Extract (40 mg/mL) + ABZ (100 mg/kg), G-4: Rhizome Extract + ABZ (100 mg/kg), G-5: Leaf Extract (10 mg/mL), G-6: Leaf Extract (40 mg/mL), G-7: Rhizome extract, G-8: Negative control. The necropsy was performed on the 45th day of experimental infection.</p><h3>Results</h3><p>The rhizome extract significantly reduced the effectiveness of ABZ compared to leaf extracts. The number of larvae in rhizome extract-treated mice (G7) was similar to ABZ-treated mice (G1) (<i>P</i> &lt; 0.05). The larval number in mice administered leaf extract (10 mg/mL and 40 mg/mL) was slightly decreased. But no significant difference was detected in the larval number depending on the dose of leaf extracts (<i>P</i> &gt; 0.05).</p><h3>Conclusion</h3><p>The administration of the leaf and rhizome extracts did not contribute to the effectiveness of ABZ. Also the effect of ABZ on the larvae number obtained from the tissues was reduced by the rhizome extract. The larvae number in the group in which rhizome extract was given alone was almost close to the group in which ABZ was administered. The results provide insight for further research on the antiparasitic properties of <i>A. rupicola</i> Boiss <i>rupicola</i> rhizome extract.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Experimental Congenital Toxoplasmosis on the Thyroid Gland: Histopathological and Immunobiochemical Indices Assessment","authors":"Hanan Abd Elgawad,&nbsp;Manar S. Elmehankar,&nbsp;Nairmen Nabih,&nbsp;Heba Sheta,&nbsp;Soha I. Awad","doi":"10.1007/s11686-024-00969-x","DOIUrl":"10.1007/s11686-024-00969-x","url":null,"abstract":"<div><h3>Purpose</h3><p>The thyroid gland is one of the most vital endocrine organs. It is responsible for the synthesis and secretion of hormones principally triiodothyronine (T3) and thyroxine (T4). These hormones play a significant role in the functions and the metabolism of the body. The thyroid gland could be affected by several infections, among them <i>Toxoplasma gondii</i>. Congenital toxoplasmosis took place when the parasite reached the developing fetus and infected any nucleated cells within it. This study assessed the effect of experimental congenital toxoplasmosis on the thyroid gland structure and function.</p><h3>Methods</h3><p>We used 34 Wistar rats and allocated them into two groups: normal control group (17 rats) and congenital toxoplasmosis group (17 rats). After euthanasia, the brain and the thyroid gland was assessed through histopathological examination. Thyroid functions examination was performed through measuring the serum levels of T3, thyroxine T4, and thyroid stimulating hormone (TSH). Moreover, serum levels of thyroid antibodies [thyroid-peroxidase antibody (TPO-Ab) and anti-thyroglobulin (TG-Ab)] were examined.</p><h3>Results</h3><p>The examination of thyroid tissues of the congenital toxoplasmosis group showed decreased or absent colloid secretion. About 47.1% of follicles showed degeneration with different grades. Parafollicular cells hyperplasia were observed in 23.6% of specimens. The serum concentrations of T3, T4, and TSH were significantly higher in congenital toxoplasmosis group than the control group. The congenital toxoplasmosis group had lower serum concentrations of TPO-Abs than the control group.</p><h3>Conclusion</h3><p>These results indicated that congenital <i>Toxoplasma</i> infection could result in a central hyperthyroidism state with alteration of thyroid gland structure in offspring.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichomoniasis in Men: A Neglected Factor in Male Infertility?","authors":"Seyed Ali Hosseini,&nbsp;Mohammad Matini,&nbsp;Maryam Bahmanzadeh,&nbsp;Reza Aslani,&nbsp;Faeze Foroughi-Parvar","doi":"10.1007/s11686-024-00984-y","DOIUrl":"10.1007/s11686-024-00984-y","url":null,"abstract":"<div><h3>Purpose</h3><p>This study is aimed to detect the frequency of trichomoniasis, a sexually transmitted infection caused by an anaerobic protozoan <i>Trichomonas vaginalis</i>, in men referred to the Fertility and Infertility Research Center Hamadan University of Medical Sciences.</p><h3>Methods</h3><p>In this cross-sectional study, a group of 197 male volunteers who sought medical attention for issues related to infertility participated. The urine and semen samples were collected in sterile conditions. Both urine and semen sediment were promptly examined under a microscopy to detect the presence of motile trophozoites. Subsequently, 50 µl of urine sediment were inoculated into the Dorset culture medium, whereas 50 µl of semen sediment were inoculated into the Diamond culture (TYI-S-33) and incubated at 35.5^oC. Finally, the processed urine samples were used for molecular analysis.</p><h3>Results</h3><p>The investigated subjects had an average age ranging from 36 to 40 years. There were 181 volunteers with fertility issues, outnumbering the 16 individuals with normal fertility. Spermogram analysis showed that 48% of participants had non-motile or progressive sperms, and 48% had abnormalities in sperm morphology. <i>T. vaginalis</i> was not detected through microscopic assessment, but PCR and sequence analysis revealed one case in a 33-year-old infertile individual, who had only 0.3% normal sperm with 19% motility. The isolated <i>T.vaginalis</i> was found to have the G genotype based on the sequencing analysis.</p><h3>Conclusion</h3><p>Trichomoniasis in males was considered unimportant, with the belief that it would improve on its own. This study suggests that parasites can be considered as one of the male infertility factors, however, the impact is not fully understood. This investigation confirmed the effectiveness of molecular techniques in detecting trichomoniasis in males.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Role of Toxoplasma gondii Infection in Multiple Sclerosis Development: A Seroepidemiological Study in North Khorasan Province, Iran","authors":"Amirali Ghahremani,&nbsp;Hasan Namdar Ahmadabad,&nbsp;Seyed Mohammad javadzadeh,&nbsp;Reza shafiei","doi":"10.1007/s11686-024-00962-4","DOIUrl":"10.1007/s11686-024-00962-4","url":null,"abstract":"<div><h3>Pourpose</h3><p>This study aimed to investigate the seroepidemiological status of Toxoplasma <i>gondii</i> (T. <i>gondii</i>) infection in Multiple Sclerosis (MS) patients compared to controls.</p><h3>Methods</h3><p>The present study included 98 MS patients and 100 controls. Demographic and clinical information collected through interviews, electronic medical records, and clinical examinations. Serum samples analyzed for anti-T. <i>gondii</i> IgG and IgM antibodies using ELISA.</p><h3>Results</h3><p>A significant difference observed in the seroprevalence of anti-T. <i>gondii</i> IgG between 49 MS patients (50%) and 33 controls (33%). MS patients with seropositive anti-T. <i>gondii</i> IgG had significantly different age, Expanded Disability Status Scale (EDSS) scores, MS phenotypes, age of diagnosis, and age of onset compared to seronegative patients. Serum levels of anti-T. <i>gondii</i> IgG significantly elevated in the case group compared to the control group (277.8 ± 28.61 vs. 137.1 ± 9.52, <i>P</i> &lt; 0.0001). In controls, all subjects were seronegative for anti-T. <i>gondii</i> IgM; three MS cases were positive, showing no significant difference in anti-T. <i>gondii</i> IgM seroprevalence between groups.</p><h3>Conclusion</h3><p>The study suggests a potential association between T. <i>gondii</i> infection and MS, indicating that anti-T. <i>gondii</i> IgG positivity may correlate with MS phenotypes and influence disease progression. This suggests that chronic toxoplasmosis may be risk factor for MS.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasma gondii Infection in the Male Reproductive System: A Systematic Review","authors":"Pablo Tabares Tejada,&nbsp;Walter D. Cardona Maya","doi":"10.1007/s11686-024-00978-w","DOIUrl":"10.1007/s11686-024-00978-w","url":null,"abstract":"<div><h3>Purpose</h3><p>Toxoplasmosis is a worldwide widespread parasitic infection; it affects about 30% of the global population, either through acute toxoplasmosis or its sequels. Even though the male reproductive system is not the primary target for <i>Toxoplasma gondii</i> (<i>T. gondii</i>), studies have inquired into the possibility of presenting repercussions in this system directly or indirectly due to toxoplasmosis. Therefore, this systematic literature review aims to summarize the available evidence on the effects of infection caused by <i>T. gondii</i> on the male reproductive tract.</p><h3>Methods</h3><p>We searched PubMed, Scopus, LILACS and Google Scholar until June 2024 to identify studies of T. gondii and the human male reproductive system. Finally, we analyzed 24 papers published between 1986 and 2024. The Joanna Briggs Institute (JBI) Critical Appraisal Checklist was used to assess the potential risk of bias and the quality of the results.</p><h3>Results</h3><p>Infertility is a multicausal issue, including various stages in which the infection caused by <i>T. gondii</i> could interfere, but the mechanisms are not fully understood yet. Studies in animals, particularly rats, have shown the harmful effects of the parasite on sperm performance and endocrine function. In the same way, sexual transmission of <i>T. gondii</i> has been extensively studied in animals, with the parasite detected in the semen of various species. In humans, this transmission route remains theoretical due to study limitations. However, discrepancies in findings call for further research to understand the mechanisms and make the <i>T. gondii</i>’s infection impact on the male reproductive system a topic of growing interest.</p><h3>Conclusion</h3><p>Acute and chronic infection by <i>T. gondii</i> in the male reproductive system is a topic of growing interest due to its possible implications for reproductive health.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approaches of Inducing Tolerance to Murine Schistosomiasis mansoni applying Biomphalaria and Bulinus Proteins","authors":"Hanan S. Mossalem,&nbsp;Sami Mohamed Nasr,&nbsp;Azza Moustafa Fahmy,&nbsp;Shimaa Atta,&nbsp;Gehan Labib Abuelenain","doi":"10.1007/s11686-025-00988-2","DOIUrl":"10.1007/s11686-025-00988-2","url":null,"abstract":"<div><h3>Background</h3><p>The freshwater snails <i>Biomphalaria alexandrina</i> and <i>Bulinus trancatus</i> are key contributors to the transmission of <i>S. </i><i>mansoni</i> and <i>S.</i><i>haematobium</i>, respectively, for being their intermediate hosts.</p><h3>Objectives</h3><p>This research study aimed to investigate the potency of the nucleoproteins (NPs) extracted from both snail species on the host immune reactions as an approach to developing a potential vaccine.</p><h3>Methods</h3><p>Three groups of six-week-old Swiss-Webster mice (<i>n</i> = 18; 15–20 g each) were injected intraperitoneally for three consecutive weeks with single doses (once a week) of <i>B. alexandrina</i>, <i>B. truncatus</i>, or a mixture of their nucleoproteins (50 µg each). On day 21st, the nucleoprotein-treated mice altogether, with six more mice, received subcutaneously <i>S. mansoni</i> cercariae (60/mouse). Eight weeks later, the experimental mice were sacrificed for evaluation of certain parasitological, molecular and immunological responses.</p><h3>Results</h3><p>The data of mice immunized with the various types of nucleoproteins showed a significant increase of FAS/R gene expressions in hepatic tissues and anti-IgG antibody levels in sera on the one hand and a significant decrease of worm loads and β-actin/R gene expression levels on the other hand when compared to the infected control mice.</p><h3>Conclusion</h3><p>These findings highlight the role of snails in immunomodulation and shed light on the possibility of antagonizing effects that might occur when the nucleoproteins of different species are mixed. Moreover, this research study might promote the literature spotting the importance of snail proteins against schistosomiasis.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Record and Identification of a Microsporidian Pathogen, Nosema Maddoxi in the Population of Brown Marmorated Stink Bug Halyomorpha Halys (Hemiptera: Pentatomidae) in Türkiye","authors":"Samet Eker,&nbsp;Mustafa Yaman,&nbsp;Ömer Ertürk,&nbsp;Tuğba Sağlam Güvendik,&nbsp;Gönül Algı","doi":"10.1007/s11686-024-00963-3","DOIUrl":"10.1007/s11686-024-00963-3","url":null,"abstract":"<div><h3>Purpose</h3><p>The brown marmorated stink bug, <i>Halyomorpha halys</i> (Hemiptera: Pentatomidae), is an invasive and a highly polyphagous species with a strong dispersal capacity. Unfortunately, there is currently no effective control method that can prevent or reduce the economic loss caused by this pest. Among natural enemies, microsporidia cause infections in insects so that they can generally shorten life span, reduce fertility and inhibit growth.</p><h3>Methods</h3><p>Current study involved field study, light and electron microscopy and molecular phylogenic analyses of a microsporidian pathogen in the populations of <i>H. halys</i> in Türkiye.</p><h3>Results</h3><p>The microsporidian infection were mostly observed in malpighian tubules and fatty tissue filled with high spore density. Fresh uninucleate spores are oval, measued as 3.73 × 2.01 µm in dimensions. The mature spore wall is relatively thick and measures 75–85 nm and consists of a clear endospore (40–50 nm) and an electron-dense, uniform, thin exospore (25–30 nm). The polar filament is isofilar, 85–110 mm in diameter and has 7–8 coils. It is found to be most closely related to <i>Nosema maddoxi</i> isolate Mn.6 isolated from <i>H. halys</i> in Georgia in phylogenetic tree.</p><h3>Conclusion</h3><p>The spore morphology and structural features of the isolate from <i>H. halys</i> identify it as <i>Nosema maddoxi</i>. The phylogenetic analyses confirm both light and electron microscopic observations. This is the first microsporidian record from <i>H. halys</i> and also from the order Hemiptera in Türkiye. It is also confirmed that the invasive pest, <i>H. halys</i> carries its natural pathogen, <i>N. maddoxi</i> to new geographical locations during its distribution.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Henneguya Species Cause Gill Disease of Commercial Amazonian Fish","authors":"Michele Velasco,&nbsp;Karoliny Caldas Xavier,&nbsp;Ana Maria Medeiros Furtado,&nbsp;Jhonata Eduard,&nbsp;José Ledamir Sindeaux-Neto","doi":"10.1007/s11686-024-00980-2","DOIUrl":"10.1007/s11686-024-00980-2","url":null,"abstract":"<div><h3>Background</h3><p>Freshwater fish are affected with much parasitic diseases, among the most common are Henneguyosis caused by myxozoans of the genus <i>Henneguya</i>, which exhibit great diversity in fish from South America, particularly in the Brazilian Amazon.</p><h3>Purpose</h3><p>In this present study, we describe the morphological and phylogenetic aspects of the small ribosomal subunit (SSU rDNA) of two new species of <i>Henneguya</i> infecting the gills from <i>Hypophthalmus marginatus</i>, a freshwater catfish from the Amazon.</p><h3>Methods</h3><p>In 148 specimens, has been observed cyst formation in different regions of the gills, intrafilamentary and intralamellar. These samples were collected for PCR amplification and phylogenetic analysis.</p><h3>Results</h3><p>The myxospores from each cyst have an elliptical spore body, consisting of two elliptical polar capsules, but differed in taxonomic morphometric measurements, such as total size, caudal length, spore body length and width, and polar capsule length and width. Phylogenetically, the species formed a clade with <i>Henneguya</i> spp. described in Siluriform fish in Brazil.</p><h3>Conclusion</h3><p>These findings support the description of two species, <i>Henneguya matosi</i> n. sp. and <i>H. marginatus</i> n. sp., which infect different regions of the gills of <i>Hypophthalmus marginatus</i>, a commercially important catfish.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Detection and Molecular Characterization of Colpodella in Goats, Foxes, and Birds","authors":"Kyriacos A. Hasapis,&nbsp;Iris Charalambidou,&nbsp;Catherine O’Dowd Phanis,&nbsp;Stefanie Kazamia,&nbsp;Nicolaos Kassinis,&nbsp;Chad Schou,&nbsp;Panagiotis Karanis","doi":"10.1007/s11686-024-00959-z","DOIUrl":"10.1007/s11686-024-00959-z","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aimed to evaluate the prevalence of <i>Colpodella</i> sp. in domestic and wild animals in Cyprus. To the authors’ knowledge, this is the first study to report the detection of <i>Colpodella</i> sp. in foxes (Cyprus red fox <i>Vulpes vulpes indutus</i>), wild birds (Eurasian coot <i>Fulica atra</i>, duck <i>Anas</i> spp.) and goats (<i>Capra hircus</i>) worldwide.</p><h3>Methods</h3><p>A total of 180 faecal samples (29 foxes, 48 Eurasian coot, 20 Eurasian teal <i>Anas crecca</i>, 7 duck, 44 goats − 10 from a farm and 34 free-living individuals - and 32 sheep <i>Ovis aries</i>) were analyzed for the parasite by nested PCR and sequencing.</p><h3>Results</h3><p>Four samples were positive (4/180 = 2.2%), including one goat from a farm (1/10 = 10%), one fox (1/29 = 3.4%), one Eurasian coot (1/48 = 2.1%) and one duck (1/7 = 14.3%).</p><h3>Conclusion</h3><p>The results of this study support the evidence that <i>Colpodella</i> sp. can infect mammals and birds, as well as livestock and wildlife, which could act as zoonotic reservoirs of the parasite and potentially pose a risk to human and animal health.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}