Acta Parasitologica最新文献

{"title":"Molecular Systematics and Type-material-based Redescription of Metarhadinorhynchus lateolabracis, With Taxonomic Revisions of Metarhadinorhynchus, Indorhynchus, and Neotegorhynchus (Acanthocephala: Palaeacanthocephala)","authors":"Yuki Kita,&nbsp;Masato Nitta,&nbsp;Hiroshi Kajihara","doi":"10.1007/s11686-024-00876-1","DOIUrl":"10.1007/s11686-024-00876-1","url":null,"abstract":"<div><h3>Purpose</h3><p>Molecular phylogenetics has been improving the acanthocephalan systematics, yet numerous taxa remain unexplored. The palaeacanthocephalan <i>Metarhadinorhynchus</i> Yamaguti, 1959 and its type species <i>M. lateolabracis</i> Yamaguti, 1959 are such to-be-explored taxa. We aim to refine (<i>i</i>) the systematic placement, (<i>ii</i>) the morphological circumscription, and (<i>iii</i>) the taxonomic components of the genus. We also aim to examine the taxonomic status of the species that have been assigned to the genus.</p><h3>Methods</h3><p>Morphological observations on newly collected specimens as well as the type material of <i>Metarhadinorhynchus lateolabracis</i> were conducted. Also, molecular phylogenetic analyses with maximum-likelihood method and Bayesian inference were performed based on freshly collected specimens. Nominal species that have at least once been assigned in <i>Metarhadinorhynchus</i>, as well as a related form, <i>Gorgorhynchus lateolabri</i> Yin and Wu, 1984, are taxonomically re-evaluated based on literature information.</p><h3>Results</h3><p>Our re-examination of the type material of <i>M</i>. <i>lateolabracis</i> revealed that the number of cement glands is six, instead of eight as described and illustrated in the original description. In the resulting phylogenetic tree, <i>M</i>. <i>lateolabracis</i> was nested in Isthmosacanthidae. <i>Gorgorhynchoides</i> Cable and Linderoth, 1963 was found to be a junior synonym of <i>Metarhadinorhynchus</i>. Taxonomic re-evaluations of six nominal species that have once belonged in <i>Metarhadinorhynchus</i> led to modifications of generic diagnoses for <i>Indorhynchus</i> Golvan, 1969 and <i>Neotegorhynchus</i> Lisitsyna, Xi, Orosová, Barčák, and Oros, 2022.</p><h3>Conclusions</h3><p><i>Metarhadinorhynchus</i> has been assigned to Leptorhynchoididae (Echinorhynchida), but our study now locates it in Isthmosacanthidae (Polymorphida). We propose 13 new combinations of specific names in <i>Metarhadinorhynchus</i> and three in <i>Indorhynchus</i>. <i>Metarhadinorhynchus lateolabri</i> (Yin and Wu, 1984) comb. nov. may be synonymous with <i>M. orientalis</i> (Wang, 1966) comb. nov.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1411 - 1425"},"PeriodicalIF":1.2,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Azygiid Reported from Africa or a Characiform Fish: Plenivitellinum kifi n. gen., n. sp. (Digenea: Azygiidae) Infecting the Gut of African Tigerfish, Hydrocynus vittatus Castelnau, 1861 (Characiformes: Alestidae) from the Kavango River","authors":"Triet Nhat Truong,&nbsp;Haley Rebecca Dutton,&nbsp;Edward Charles Netherlands,&nbsp;Louis Heyns DuPreez,&nbsp;Francois Jakob Jacobs,&nbsp;Stephen Ashton Bullard","doi":"10.1007/s11686-024-00872-5","DOIUrl":"10.1007/s11686-024-00872-5","url":null,"abstract":"<div><h3>Purpose</h3><p>This paper aims to describe <i>Plenivitellinum kifi</i> n. gen., n. sp. (Digenea: Azygiidae) infecting the gastrointestinal tract of the African tigerfish, <i>Hydrocynus vittatus</i> Castelnau, 1861 (Characiformes: Alestidae) in the Kavango River, Namibia. We revise the diagnosis of Azygiidae Lühe, 1909 to accommodate this new species.</p><h3>Methods</h3><p>The worm was heat-killed, fixed in 10% neutral buffered formalin, stained in Van Cleave’s and Ehrlich’s hematoxylins, cleared in clove oil, and mounted on glass slide using Canada balsam.</p><h3>Results</h3><p>The new azygiid resembles species of <i>Otodistomum</i> Stafford, 1904 by having an elongate body, a ventral sucker that is wider than the oral sucker and that is in the anterior half of the body, a pre-testicular ovary, a uterus that primarily occupies the inter-caecal space between the ovary and the ventral sucker, and a vitellarium that is restricted to the hindbody and that is confluent posteriorly in the post-testicular region. The new genus differs from all species of Azygiidae by having the combination of a ventral sucker that is wider than the oral sucker (vs. narrower), an elongate prostatic sac that extends posteriad to near the posterior margin of the ventral sucker (vs. an ovoid prostatic sac that is wholly anterior to or slightly overlaps the anterior margin of the ventral sucker), a vitellarium that nearly fills the post-testicular space and that extends posteriad beyond the caecal tips (vs. a vitellarium that is separate posteriorly or that is restricted to the anterior half of the post-testicular space and does not extend posteriad beyond the caecal tips), and an I-shaped excretory bladder (vs. Y-shaped).</p><h3>Conclusion</h3><p>This study documents the first record of an azygiid from Africa and the first record of an azygiid infecting a characiform fish.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1403 - 1410"},"PeriodicalIF":1.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00872-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription of Raphidascaris (Ichthyascaris) vicentei (Nematoda: Raphidascarididae) Parasitizing Lutjanid Fishes (Actinopterygii: Lutjanidae) from the Northeast Coast of Brazil, Including First Observation Using SEM","authors":"André Mota Alves,&nbsp;Felipe Bisaggio Pereira,&nbsp;Luiz Eduardo Roland Tavares,&nbsp;Ricardo Massato Takemoto,&nbsp;Claudia Moura de Melo,&nbsp;Rubens Riscala Madi,&nbsp;Verónica de Lourdes Sierpe Jeraldo","doi":"10.1007/s11686-024-00865-4","DOIUrl":"10.1007/s11686-024-00865-4","url":null,"abstract":"<div><h3>Purpose</h3><p>In this study, 143 lutjanid fishes representing four different species from the Northeast coast off Brazil were analyzed for parasites. The aim of the present study is to provide a detailed redescription of <i>R.</i> (<i>I</i>.) <i>vicentei</i>, collected from lutjanid fishes in Brazil, including the first observation of the species using scanning electron microscopy (SEM).</p><h3>Methods</h3><p>Nematodes found clarified in a crescent glycerin series, were photographed and measured (in mm), and some specimens destined for SEM. The Illustrations were made using an optical microscope with a drawing tube.</p><h3>Results</h3><p>Nematodes found were identified as <i>Raphidascaris</i> (<i>Ichthyascaris</i>) <i>vicentei</i>, infecting 6.29% of the examined fish. Detailed morphological analysis revealed the following characteristics observed for the first time in this species: labial papillae, narrow lateral alae and the detailed structure of male spicules. These contributions help filling the gaps in the literature and expand the knowledge of this parasite morphology.</p><h3>Conclusions</h3><p>The presence of <i>R. (I</i>.<i>) vicentei</i> in four lutjanid species may indicates low host specificity, highlighting its adaptability to different hosts. Furthermore, new host records are given. This research provides the most comprehensive morphological description of <i>R. (I</i>.<i>) vicentei</i> to date, generating crucial insights for a better understanding of the parasitic biodiversity off the Brazilian Northeastern coast of Brazil, as well as establishing foundation for future investigations on the ecology and impacts of these parasites in fish populations from this region.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1396 - 1402"},"PeriodicalIF":1.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Oxidative Stress, Thyroid Hormones, Trace Elements and Some Biochemical Markers in Goats Naturally Infected with Theileria ovis","authors":"Yunus Emre Caglar,&nbsp;Yeter Deger,&nbsp;Bekir Oguz","doi":"10.1007/s11686-024-00866-3","DOIUrl":"10.1007/s11686-024-00866-3","url":null,"abstract":"<div><h3>Background</h3><p>Theileriosis is a tick-borne disease caused by protozoon species in the <i>Theileria</i> genus of the Theileriidae family. The biochemical changes induced by infection are considered to be an important understanding of the pathophysiology of caprine theileriosis. In this study, it was aimed to determine oxidative stress, thyroid hormones, trace elements, and biochemical parameters in theileriosis infection.</p><h3>Materials and Methods</h3><p>A sample of 14 goat was used for this purpose, of which 7 were healthy and 7 were infected with <i>Theileria ovis</i>. <i>Theileria</i> infection was diagnosed from the polymerase chain reaction (PCR). Sera from blood samples was tested for total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), alanine aminotransferase (ALT), aspartate transaminase (AST), gamma glutamyl transferase (GGT), total protein, albumin, triglyceride, cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), urea, blood urea nitrogen (BUN), creatinine, triiodothyronine (T3), thyroxine (T4), copper (Cu), zinc (Zn), cobalt (Co), manganese (Mn), selenium (Se), iron (Fe).</p><h3>Result</h3><p>TOC, OSI, AST, ALT and GGT values were higher in the patient group than in the healthy group (<i>P</i> &lt; 0.05). On the other hand, there were decreases in TAC, T3, T4, total protein, albumin, creatinine, Cu, Zn, Se, and Co values (<i>P</i> &lt; 0.05). However, there was not found to be a statistical difference between the healthy and patient groups in terms of triglyceride, cholesterol, HDL, LDL, urea, BUN, Mn, and Fe values (<i>P</i> &gt; 0.05).</p><h3>Conclusions</h3><p>It can be stated that oxidative stress is a complication of caprine theileriosis and it may be accompanied with hypothyroidism and deficits in trace minerals.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1389 - 1395"},"PeriodicalIF":1.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Diagnosis of Oestrus ovis (Linnaeus, 1758) Larvae Causing Conjunctival Ophthalmomyiasis in Mexico City, Mexico","authors":"Carolina Isabel Olivera-Pérez,&nbsp;Omar Lagunas-Calvo,&nbsp;Vianney Cortés-González,&nbsp;Alejandro Oceguera-Figueroa,&nbsp;Luis García-Prieto,&nbsp;Samuel Peña-Ortiz,&nbsp;Luis Alfonso Hernández-Piñamora,&nbsp;Miriam Erandi Reyna-Fabián","doi":"10.1007/s11686-024-00862-7","DOIUrl":"10.1007/s11686-024-00862-7","url":null,"abstract":"<div><h3>Purpose</h3><p>Human ophthalmomyiasis is a rare ocular parasitosis that results from the accidental infestation of dipteran larvae of several species, including <i>Oestrus ovis</i> (Linnaeus, 1758). This study aims to present the fourth documented human case of ophthalmomyiasis in Mexico, identifying the etiological agent through morphological and molecular analyses. Additionally, we investigated the phylogenetic position and genetic distances among different specimens globally characterized based on mitochondrial <i>Cox1</i> sequences.</p><h3>Methods</h3><p>A total of five larval specimens were extracted from the patient’s eye, with two specimens allocated for identification based on morphological features using a stereomicroscope, and the remaining three preserved in absolute ethanol, one of them used for subsequent analysis using molecular methods. The mitochondrial <i>Cox1</i> region was amplified and sequenced using automated Sanger sequencing. The resulting sequence was deposited in GenBank under accession number OR440699 and subjected to BlastN analysis against 35 other <i>Cox1</i> sequences of <i>O. ovis</i> from GenBank. The identity and phylogenetic position of the strains were further explored using parsimony and maximum likelihood phylogenetic methods.</p><h3>Results</h3><p>Morphological examination of the larval specimens extracted from the patient’s eye unequivocally identified them as <i>O. ovis</i> species. BlastN analysis and comprehensive phylogenetic investigations involving a total of 36 <i>Cox1</i> sequences confirmed the taxonomic identity of the larvae. Notably, our sequence was positioned within the cluster formed by the Brazilian and two Iranian samples. This finding underscores a shared genetic ancestry among these distinct geographical isolates and provides valuable insights into the evolutionary relationships within <i>O. ovis</i> populations.</p><h3>Conclusion</h3><p>The presence of <i>O</i>. <i>ovis</i> infestation in Mexico City suggests potential shifts in environmental conditions favoring fly proliferation, highlighting the need for vigilance in urban healthcare settings.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1382 - 1388"},"PeriodicalIF":1.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Seroprevalence and Risk Factors of Heartworm Infection for Dogs in Rio de Janeiro with Access to Veterinary Care","authors":"Mariana Guedes,&nbsp;Tamiris Gomes,&nbsp;Bruno Alberigi,&nbsp;Emilene Prudente,&nbsp;Alexandre Bendas,&nbsp;Thalita Souza,&nbsp;Flavya Mendes-de-Almeida,&nbsp;Fabiana Batalha Knackfuss,&nbsp;Alexandre Merlo,&nbsp;Norma Labarthe","doi":"10.1007/s11686-024-00859-2","DOIUrl":"10.1007/s11686-024-00859-2","url":null,"abstract":"<div><p>Heartworm infection is a chronic disease with clinical signs and effects ranging from an asymptomatic condition to severe disease and death. The prevalence of heartworm disease in the state of Rio de Janeiro has been reported to be high (21.3%). The present study was conducted to evaluate the seroprevalence and risk factors of heartworm infection for the canine population with access to veterinary services in different areas of the state of Rio de Janeiro, Brazil. A total of 1787 canine blood samples were obtained from 135 practices across 8 different areas of Rio de Janeiro state (Rio de Janeiro municipality, São Gonçalo municipality, Niterói municipality, Baixada Fluminense, and the northern, southern, eastern, and mountainous areas) and tested for the presence of <i>Dirofilaria immitis</i> antigens and antibodies against several tick-borne disease pathogens using a commercial immunochromatography technique (Vetscan® Flex 4 Rapid Test; Zoetis; NJ USA). Pet owners reported living conditions, physical characteristics, demographics, and clinical signs for evaluation of risk factors for heartworm infection. Only two evaluated risk factors were shown to enhance the risk for <i>D. immitis</i> infection, including having a short hair coat vs. having a medium or long hair coat (OR 2.62) or positive for antibodies to tick-borne disease parasites (OR 3.83). Clinical signs reported for dogs with heartworm disease were typical for that condition. The overall prevalence of heartworm disease in the state was 8.2%, ranging from 2.4% in the mountainous region to 29.4% in the eastern area. It could not be determined if veterinarians were not diligent about dispensing heartworm preventatives or if poor levels of compliance by dog owners were responsible for higher infection rates in some areas of the state.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1364 - 1371"},"PeriodicalIF":1.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Identification and Clinical Presentation of Crenosomosis in a Dog from Slovakia","authors":"Michaela Kaduková,&nbsp;Martin Kožár,&nbsp;Andrea Schreiberová,&nbsp;Barbora Šišková,&nbsp;Gabriela Štrkolcová","doi":"10.1007/s11686-024-00861-8","DOIUrl":"10.1007/s11686-024-00861-8","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Crenosoma vulpis</i> (Dujardin,1845) is a lungworm which has spread worldwide in canines and is associated with upper respiratory infections. In a majority of cases, the infections are accompanied with chronic cough. Diagnosis of lungworms is often underdiagnosed and can be misinterpreted as other respiratory diseases.</p><h3>Methods</h3><p>The Small Animal Clinic of the University Veterinary Hospital admitted an 11-month-old dog presented with persistent cough associated with difficulty in breathing and even asphyxia. Based on clinical symptoms, the patient underwent radiological and bronchoscopic examination. Bronchoscopy revealed the presence of lungworms obturating the branches of the tracheobronchial tree. Larvae were collected by bronchoscopic lavage and subjected to parasitological and molecular examination.</p><h3>Results</h3><p>Microscopic detection and morphological identification of the worms removed during the bronchoscopy confirmed the presence of female adult worms. The subsequent molecular characterisation of the mitochondrial (cytochrome c oxidase subunit I gene (cox1) and 12S ribosomal DNA (rDNA)), nuclear (18S rDNA) genes, as well as the analysis of the second internal transcribed spacer (ITS-2) region of the ribosomal DNA, confirmed the <i>Crenosoma vulpis</i> species<i>.</i> Faecal samples were processed using the Baermann method, which confirmed the presence of the larval stage 1 of <i>C.</i> <i>vulpis</i>. The therapy with fenbendazole at a dose of 50 mg/kg of live weight once daily for the period of 7 days was initiated for the patient.</p><h3>Conclusion</h3><p>This paper presents the first molecularly confirmed clinical case of a <i>Crenosoma vulpis</i> infection in an 11-month-old female dog of the Miniature Schnauzer breed in Slovakia.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1372 - 1381"},"PeriodicalIF":1.2,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Isosporan (Apicomplexa: Eimeriidae) from the Pacific Blue-Tailed Skink, Emoia caeruleocauda (Sauria: Scincidae: Eugongylinae), from Guam, U.S. Territory","authors":"Chris T. McAllister,&nbsp;John A. Hnida,&nbsp;Diane Vice,&nbsp;Samuel Fisher,&nbsp;Robert N. Fisher","doi":"10.1007/s11686-024-00860-9","DOIUrl":"10.1007/s11686-024-00860-9","url":null,"abstract":"<div><h3>Purpose</h3><p>Nothing is known about coccidians (Apicomplexa: Eimeriidae) from the Pacific blue-tailed skink, <i>Emoia caeruleocauda</i>. Here, we report mensural and morphometric data on a new species of <i>Isospora</i> from <i>E. caeruleocauda</i> from Guam, US Territory.</p><h3>Methods</h3><p>Feces from four <i>E. caeruleocauda</i> collected by hand in November 2023 were placed in individual vials containing 2.5% potassium dichromate. They were examined for sporulated oocysts after flotation in Sheather’s sugar solution, measured, and photographed.</p><h3>Results</h3><p>A single (25%) <i>E. caeruleocauda</i> was found to be passing oocysts representing a new species of <i>Isospora</i>. Oocysts of <i>Isospora guamensis</i> n. sp. are ellipsoidal to ovoidal with a bi-layered wall, measure (L × W) 16.5 × 11.8 μm, and have a length/width (L/W) ratio of 1.4; a micropyle and an oocyst residuum were absent but a polar granule was present. Sporocysts are ovoidal and measure 9.4 × 6.5 μm, L/W 1.4; Stieda and sub-Stieda bodies were present but a para-Stieda body was absent. The sporocyst residuum is composed various-sized granules in a compact rounded or irregular mass, sometimes dispersed between the sporozoites. The new species can be differentiated from all other isosporans from skinks by possessing the smallest oocysts known from this host family.</p><h3>Conclusion</h3><p>This is the first time an isosporan coccidian has been reported from <i>E. caeruleocauda</i> as well as the first report of a coccidian from a Guam-inhabiting skink.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1359 - 1363"},"PeriodicalIF":1.2,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Report of Tritrichomonas Foetus and Tetratrichomonas Buttreyi in Raccoon Dogs (Nyctereutes Procyonoides) in China","authors":"Dong-qian Chen,&nbsp;Qiu-yue Wang,&nbsp;Qiao-qiao Li,&nbsp;Xin-yu Luo,&nbsp;Xing-hua Wu,&nbsp;Ji-pu Wang,&nbsp;Si-chao Gao,&nbsp;Xin-chao Liu,&nbsp;WenChao Li","doi":"10.1007/s11686-024-00858-3","DOIUrl":"10.1007/s11686-024-00858-3","url":null,"abstract":"<div><h3>Background</h3><p>In recent years, the trichomonosis in raccoon dogs in China had occurred frequently. <i>Pentatrichomonas hominis</i> had been described in raccoon dogs in China in some previous studies.</p><h3>Purpose To Reveal</h3><p>whether raccoon dogs can be infected by other trichomonad species besides <i>P. hominis</i>, and clarify the prevalence and species distribution of trichomonad in raccoon dogs.</p><h3>Methods</h3><p>Herein, the 389 fecal samples were collected from farm-raised raccoon dogs in Hebei Province, all the samples were detected using the microscopic examination and several fecal samples containing trichomonad-like organisms were processed, cultured, stained, and photographed. Meanwhile, all the samples were screened by the species-specific nested PCR based on the small subunit rRNA (SSU rRNA) gene of <i>P. hominis</i>,<i>Tritrichomonas foetus</i> and <i>Tetratrichomonas buttreyi</i>, respectively, and all positive secondary PCR amplications obtained in this study were sequenced, aligned and analysed.</p><h3>Results</h3><p>62 fecal samples (15.9%,62/389) were trichomonad-positive under light microscopy, and the trichomonad-like cells were clearly observed in the culture contents. The PCR results showed that 100 samples were trichomonad-positive, including 45 <i>P. hominis</i>-positive samples (11.6%,45/389), 32 <i>T. foetus</i>-positive samples (8.2%,32/389), and 33 <i>T. buttreyi</i>-positive samples (8.5%,33/389), respectively. Double mixed infections were observed in 10 samples. The prevalence of <i>T. foetus</i> and <i>P. hominis</i> were both significantly higher in raccoon dogs with diarrhea (13.9%, and 25.0%) than that in raccoon dogs without diarrhea (7.6%, and 9.3%) (<i>p</i> &lt; 0.05).All samples confirmed as trichomonad-positive under microscopy were also found to be trichomonad-positive by PCR analysis. The sequencing and phylogenetic analysis demonstrated the sequences obtained in this study belonged to <i>P. hominis</i>, <i>T. foetus</i> and <i>T. buttreyi</i> SSU rRNA, respectively. Among them, the <i>T. buttreyi</i> SSU rRNA sequences obtained in this study harbored the new sequence polymorphisms. Based on preliminary morphological and molecular analyses, raccoon dogs are considered as the new host of <i>T. foetus</i> and <i>T. buttreyi</i>.</p><h3>Conclusion</h3><p>This is the first report about the identifcation and prevalence of <i>T. foetus</i> and <i>T. buttreyi</i> in raccoon dogs in China, and the results increase our knowledge about the host range and prevalence of trichomonad species.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1352 - 1358"},"PeriodicalIF":1.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Haplotypes of Blastocystis sp. Identified in Faeces from Various Animal Groups in Algeria","authors":"Asma Guilane,&nbsp;Ikram Haleche,&nbsp;Fadila Tazerouti,&nbsp;Hocine Ziam,&nbsp;Tahar Kernif,&nbsp;Amina Boutellis","doi":"10.1007/s11686-024-00857-4","DOIUrl":"10.1007/s11686-024-00857-4","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Blastocystis</i> sp. is a single-celled, anaerobic, parasitic protozoan commonly found in the intestinal tract of animals and humans globally. Genetic analysis has revealed significant diversity within its species, leading to the identification of at least 40 subtypes (ST1–ST40). This study aimed to identify and differentiate <i>Blastocystis</i> in faeces samples from various animal hosts in Algeria.</p><h3>Methods</h3><p>A total of 403 fecal samples, collected from both domestic and zoo animals, were subjected to PCR amplification and sequencing of <i>Blastocystis</i>-specific small subunit ribosomal RNA (<i>SSU-RNA</i>) gene.</p><h3>Results</h3><p>The overall prevalence of <i>Blastocystis</i> in animals was found to be 38.9%. Through comprehensive phylogenetic and phylogeographic analyses, we identified four distinct subtypes (ST1 in both domestic and zoo animals, and ST3, ST4, and ST5 exclusively in zoo animals), encompassing nine different haplotypes, including five that appear original to Algeria.</p><h3>Conclusion</h3><p>This study represents the first epidemiological molecular investigation of <i>Blastocystis</i> sp. in animals in Algeria.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1338 - 1351"},"PeriodicalIF":1.2,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}