The Effects of Extracellular Vesicles Derived from Hydatid Cyst Fluid on the Expression of microRNAs Involved in Liver Fibrosis

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-04-12 DOI:10.1007/s11686-025-01024-z

Hanieh Mohammad Rahimi, Fatemeh Mahdavi, Nasim Eslami, Sara Nemati, Hamed Mirjalali

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引用次数: 0

Abstract

Introduction

Hydatidosis is a zoonotic neglected disease caused by the larval stage of Echinococcus granulosus. Evidence suggests a communication between hydatid cyst (HC) and hosts via extracellular vesicles (EVs). However, a little is known about the communication between EVs derived from HC fluid (HCF) and host cells. The current study aimed to investigate the effect of HCF derived EVs on expression of fibrotic and anti-fibrotic miRNAs in THP-1 cell line.

Methods

In the current study, EVs were isolated using ultracentrifugation from wild-infected sheep HCF and characterized by western blot, electron microscope, and size distribution analysis. The effects of EVs on the expression levels of microRNAs (mir-16, mir-29a, and mir-155) involved in liver fibrosis were investigated using quantitative real-time PCR (qRT-PCR), 3 and 24 h after incubation.

Results

Western blot analyses confirmed the expression of CD63 marker, while Calnexin and CD81 were absent in EVs samples. The SEM and morphology revealed round shape vesicles. The DLS analysis showed average size distribution 130.6 nm diameter. The expression levels of mir-16 and mir-29a were significantly upregulated after 3 h for 8.66 and 3.420, respectively, while they were significantly downregulated after 24 h for 3.853 and 1.859, respectively.

Conclusion

The main mechanism of the communication between EVs derived from HCF and their host remains unclear. Our results suggest that HC may modulate the expression of miRNAs, involved in liver fibrosis via EVs.

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来源于包虫囊液的细胞外小泡对参与肝纤维化的microrna表达的影响

摘要包虫病是由细粒棘球绦虫幼虫期引起的一种被忽视的人畜共患疾病。有证据表明，包虫病（HC）与宿主之间通过细胞外囊泡（EVs）进行交流。然而，关于来自HC液（HCF）的ev与宿主细胞之间的通讯知之甚少。本研究旨在探讨HCF衍生的ev对THP-1细胞系纤维化和抗纤维化mirna表达的影响。方法本研究采用超离心方法从野生感染的羊HCF中分离出ev，并采用western blot、电镜和大小分布分析对其进行鉴定。利用实时荧光定量PCR (qRT-PCR)，在孵育后3和24小时研究ev对参与肝纤维化的microrna （mir-16、mir-29a和mir-155）表达水平的影响。结果免疫印迹分析证实，EVs样品中CD63标记物表达，Calnexin和CD81缺失。扫描电镜和形态学显示圆形囊泡。DLS分析显示平均粒径分布为直径130.6 nm。mir-16和mir-29a的表达水平在3 h后分别为8.66和3.420显著上调，在24 h后分别为3.853和1.859显著下调。结论HCF源性EVs与宿主间通讯的主要机制尚不清楚。我们的研究结果表明，HC可能通过ev调节参与肝纤维化的mirna的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.