Molecular Investigation of Anaplasma spp. and Genotype Profile of A. ovis in Sheep from Different Farms in Türkiye

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-04-10 DOI:10.1007/s11686-025-01021-2

Özge Dülek, Çağrı Kandemir, Ecem Su Koçkaya, Ecem Sürgeç, Mervenur Güvendi, Muhammet Karakavuk, Aysu Değirmenci Döşkaya, Özlem Günay-Esiyok, Turgay Taşkın, Mert Döşkaya, Cemal Ün, Adnan Yüksel Gürüz, Ahmet Efe Köseoğlu, Sedef Erkunt Alak, Erkan Pehlivan, H. Deniz Şireli, Serdar Koçak, Ömer Faruk Yılmaz, Hüseyin Can

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引用次数: 0

Abstract

Purpose

Anaplasmosis is a tick-borne disease which is caused by different Anaplasma species. Among Anaplasma species, A. ovis which can infect sheep and goats cause ovine anaplasmosis. In this study, the prevalence of Anaplasma spp. was investigated in 31 different breeds of sheep from different regions of Türkiye.

Method

Anaplasma spp. was investigated by PCR targeting MSP-4 gene in blood samples of sheep breeds (n = 366) collected from different regions of Türkiye. Also, some Anaplasma spp. positive samples were sequenced for species identification and sub-cluster analyses.

Results

The molecular prevalence of Anaplasma spp. was 43.9% (161/366). In Anatolian Merino (n = 10) and Akkaraman (n = 11) breeds, the molecular prevalence of Anaplasma spp. reached to 100%. Also, the highest molecular prevalence was detected in Black Sea region by 70% (28/40) and the lowest molecular prevalence was detected in Marmara region by 32% (16/50). While the prevalence of Anaplasma spp. was 59.7% in sheep produced in the extensive system, it was found as 39.2% and 9.8% in sheep produced in semi-extensive and intensive systems, respectively. Accordingly, these findings suggest that the production of sheep in the intensive system protects them from tick-borne diseases, which are of great economic importance. According to BLAST results, all sequenced Anaplasma spp. positive samples (n = 29) were identified as A. ovis. Also, mixed infections were detected in 6 positive samples. The phylogenetic tree constructed by 38 sequence data showed the presence of three different sub-clusters for A. ovis (Sub-cluster 1, 2, and 3). Sub-cluster 2 was found as the most prevalent sub-cluster with 42.1% frequency compared to the other sub-clusters.

Conclusion

This study showed that sheep grown in different regions of Türkiye have a high molecular prevalence value for Anaplasma spp.

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基耶省不同养殖场绵羊无原体及其基因型分析

目的无形体病是由不同种类的无形体引起的蜱传疾病。在无原体种类中，可感染绵羊和山羊的绵羊无原体引起绵羊无原体病。在本研究中，我们调查了来自斯里兰卡不同地区的31个不同品种的绵羊无原体的流行情况。方法采用聚合酶链反应（PCR）对366只来自新疆不同地区的绵羊品种进行MSP-4基因检测。对部分阳性标本进行了物种鉴定和亚聚类分析。结果无原体的分子感染率为43.9%（161/366）。在安纳托利亚美利奴（n = 10）和阿卡拉曼（n = 11）品种中，无原体的分子流行率达到100%。黑海地区分子患病率最高，为70%(28/40)，马尔马拉地区分子患病率最低，为32%（16/50）。粗放型养殖的绵羊无原体感染率为59.7%，半粗放型和集约型养殖的绵羊无原体感染率分别为39.2%和9.8%。因此，这些发现表明，在集约化系统中生产绵羊可以保护它们免受蜱传疾病的侵害，这对经济具有重要意义。根据BLAST结果，所有测序阳性样本（n = 29）均鉴定为卵巢无原体。6例阳性标本中检出混合感染。利用38条序列数据构建的系统发育树显示，紫斑拟南藜存在3个不同的亚簇（亚簇1、2和3），其中亚簇2的频率最高，为42.1%。结论本研究表明，生长在 rkiye不同地区的绵羊对无原体具有较高的分子流行价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.