{"title":"基于印度Tams1基因的牛环孢杆菌分子流行病学及基因型多样性研究","authors":"Rashmi Malik, Biswa Ranjan Maharana, Anita Ganguly, Sukhdeep Vohra, Binod Kumar, Ankit Kumar, Harpreet Singh, Sandeep Potliya, Sujoy Khanna, Subhasish Sahu","doi":"10.1007/s11686-025-01014-1","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Bovine theileriosis, caused by <i>Theileria annulata</i>, presents a significant economic challenge to India’s livestock industry. This study investigates the molecular epidemiology of <i>T. annulata</i> in bovine populations from Haryana, India, focusing on the genetic diversity of the Tams1 gene (785 bp).</p><h3>Methods</h3><p>The prevalence of <i>T. annulata</i> was evaluated in 800 blood samples using microscopy and PCR techniques, specifically targeting SSU rRNA and <i>Tams1</i> genes. Genetic diversity among <i>T. annulata</i> isolates was analyzed through DNA sequencing and phylogenetic studies. Additionally, the impact of various risk factors on <i>T. annulata</i> infection was assessed.</p><h3>Results</h3><p>Microscopy revealed that 29.25% (117/400) of cattle and 2.75% (11/400) of buffalo were detected positive for <i>Theileria</i> spp. in Giemsa-stained blood smears. The <i>Theileria</i> genus-specific primers amplified a 1098 bp fragment of the SSU rRNA gene in 39.5% (158/400) cattle and 6% (24/400) buffalo. The <i>T. annulata-</i>specific PCR targeting the partial <i>Tams1</i> gene revealed a prevalence rate of 37% (148/400) in cattle and 3.5% (14/400) in buffalo. A genetic analysis of the <i>Tams1</i> gene in 389 sequences, including 14 <i>T. annulata</i> isolates from Haryana (8 from cattle, 6 from buffaloes), revealed significant variation within Indian <i>T. annulata</i> parasites. Analysis of <i>Tams1</i> gene sequences (389 from 16 countries) revealed 35 haplotypes globally, with four identified from the 14 isolates sequenced in this study. Nucleotide homology among 14 northern Indian isolates ranged from 89.46–100%, compared to a broader range of 78.42–100% when global sequences were included. Among 35 haplotypes, Hap_1 is the most dominant and shows geographic clustering. Globally, low genetic distance (Fst < 0.15) and high gene flow (Nm > 1) were observed among the five populations (South Asia, East Asia, West Asia, Europe, and Africa), suggesting minimal genetic differentiation among <i>T. annulata</i> populations. Negative values in Tajima’ s D (-1.21941) and Li’s F (-2.97801) tests suggest recent population expansion. Risk factors such as age, sex, and host species are significantly associated with <i>T. annulata</i> infection.</p><h3>Conclusion</h3><p>This study offers comprehensive insights into <i>T. annulata</i> genetic diversity, population structure, and haplotype networks using the <i>Tams1</i> gene.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 2","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Epidemiology and Genotypic Diversity of Theileria annulata among Bovines Based Upon Tams1 Gene in India\",\"authors\":\"Rashmi Malik, Biswa Ranjan Maharana, Anita Ganguly, Sukhdeep Vohra, Binod Kumar, Ankit Kumar, Harpreet Singh, Sandeep Potliya, Sujoy Khanna, Subhasish Sahu\",\"doi\":\"10.1007/s11686-025-01014-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Bovine theileriosis, caused by <i>Theileria annulata</i>, presents a significant economic challenge to India’s livestock industry. This study investigates the molecular epidemiology of <i>T. annulata</i> in bovine populations from Haryana, India, focusing on the genetic diversity of the Tams1 gene (785 bp).</p><h3>Methods</h3><p>The prevalence of <i>T. annulata</i> was evaluated in 800 blood samples using microscopy and PCR techniques, specifically targeting SSU rRNA and <i>Tams1</i> genes. Genetic diversity among <i>T. annulata</i> isolates was analyzed through DNA sequencing and phylogenetic studies. Additionally, the impact of various risk factors on <i>T. annulata</i> infection was assessed.</p><h3>Results</h3><p>Microscopy revealed that 29.25% (117/400) of cattle and 2.75% (11/400) of buffalo were detected positive for <i>Theileria</i> spp. in Giemsa-stained blood smears. The <i>Theileria</i> genus-specific primers amplified a 1098 bp fragment of the SSU rRNA gene in 39.5% (158/400) cattle and 6% (24/400) buffalo. The <i>T. annulata-</i>specific PCR targeting the partial <i>Tams1</i> gene revealed a prevalence rate of 37% (148/400) in cattle and 3.5% (14/400) in buffalo. A genetic analysis of the <i>Tams1</i> gene in 389 sequences, including 14 <i>T. annulata</i> isolates from Haryana (8 from cattle, 6 from buffaloes), revealed significant variation within Indian <i>T. annulata</i> parasites. Analysis of <i>Tams1</i> gene sequences (389 from 16 countries) revealed 35 haplotypes globally, with four identified from the 14 isolates sequenced in this study. Nucleotide homology among 14 northern Indian isolates ranged from 89.46–100%, compared to a broader range of 78.42–100% when global sequences were included. Among 35 haplotypes, Hap_1 is the most dominant and shows geographic clustering. Globally, low genetic distance (Fst < 0.15) and high gene flow (Nm > 1) were observed among the five populations (South Asia, East Asia, West Asia, Europe, and Africa), suggesting minimal genetic differentiation among <i>T. annulata</i> populations. Negative values in Tajima’ s D (-1.21941) and Li’s F (-2.97801) tests suggest recent population expansion. Risk factors such as age, sex, and host species are significantly associated with <i>T. annulata</i> infection.</p><h3>Conclusion</h3><p>This study offers comprehensive insights into <i>T. annulata</i> genetic diversity, population structure, and haplotype networks using the <i>Tams1</i> gene.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 2\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-025-01014-1\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-025-01014-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Epidemiology and Genotypic Diversity of Theileria annulata among Bovines Based Upon Tams1 Gene in India
Purpose
Bovine theileriosis, caused by Theileria annulata, presents a significant economic challenge to India’s livestock industry. This study investigates the molecular epidemiology of T. annulata in bovine populations from Haryana, India, focusing on the genetic diversity of the Tams1 gene (785 bp).
Methods
The prevalence of T. annulata was evaluated in 800 blood samples using microscopy and PCR techniques, specifically targeting SSU rRNA and Tams1 genes. Genetic diversity among T. annulata isolates was analyzed through DNA sequencing and phylogenetic studies. Additionally, the impact of various risk factors on T. annulata infection was assessed.
Results
Microscopy revealed that 29.25% (117/400) of cattle and 2.75% (11/400) of buffalo were detected positive for Theileria spp. in Giemsa-stained blood smears. The Theileria genus-specific primers amplified a 1098 bp fragment of the SSU rRNA gene in 39.5% (158/400) cattle and 6% (24/400) buffalo. The T. annulata-specific PCR targeting the partial Tams1 gene revealed a prevalence rate of 37% (148/400) in cattle and 3.5% (14/400) in buffalo. A genetic analysis of the Tams1 gene in 389 sequences, including 14 T. annulata isolates from Haryana (8 from cattle, 6 from buffaloes), revealed significant variation within Indian T. annulata parasites. Analysis of Tams1 gene sequences (389 from 16 countries) revealed 35 haplotypes globally, with four identified from the 14 isolates sequenced in this study. Nucleotide homology among 14 northern Indian isolates ranged from 89.46–100%, compared to a broader range of 78.42–100% when global sequences were included. Among 35 haplotypes, Hap_1 is the most dominant and shows geographic clustering. Globally, low genetic distance (Fst < 0.15) and high gene flow (Nm > 1) were observed among the five populations (South Asia, East Asia, West Asia, Europe, and Africa), suggesting minimal genetic differentiation among T. annulata populations. Negative values in Tajima’ s D (-1.21941) and Li’s F (-2.97801) tests suggest recent population expansion. Risk factors such as age, sex, and host species are significantly associated with T. annulata infection.
Conclusion
This study offers comprehensive insights into T. annulata genetic diversity, population structure, and haplotype networks using the Tams1 gene.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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