{"title":"用于弓形虫病诊断的多表位抗原的设计:一种计算机方法","authors":"Negar Asadi, Leila Navapour, Navid Mogharrab, Elham Yousefi, Shahram Khademvatan, Mortaza Taheri-Anganeh","doi":"10.1007/s11686-025-01027-w","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Toxoariasis is recognized as one of the most widespread diseases affecting both animals and humans, exhibiting a global distribution. In light of the public health challenges it poses, particularly for children infected with Toxocara canis, the development of effective diagnostic tests or vaccines is of paramount importance. Serological methods are routinely employed to detect specific anti-Toxocara antibodies in infected individuals. This bioinformatics study aims to formulate a multi-epitope protein for Toxocara canis, leveraging various immunoinformatics web servers to enhance the accuracy of serodiagnosis.</p><h3>Methods</h3><p>The identification of linear and conformational B-cell epitopes for the antigens TES-26, TES-30, and TES-120 was conducted using the ABCpred and BepiPred servers. Various web servers were employed to evaluate antigenicity, solubility, and physicochemical properties, as well as to examine secondary and tertiary structures, enhance the three-dimensional model, and confirm the findings.</p><h3>Results</h3><p>This process led to the identification of conformational B-cell epitopes, aimed at exploring possible protein-antibody interactions.</p><h3>Conclusion</h3><p>Ultimately, further experimental investigations are essential for this multi-epitope construct before it can be incorporated into commercial serodiagnosis kits.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 2","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Designing of a Multi-Epitope Antigen for Toxocariasis Diagnosis: An in Silico Approach\",\"authors\":\"Negar Asadi, Leila Navapour, Navid Mogharrab, Elham Yousefi, Shahram Khademvatan, Mortaza Taheri-Anganeh\",\"doi\":\"10.1007/s11686-025-01027-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Toxoariasis is recognized as one of the most widespread diseases affecting both animals and humans, exhibiting a global distribution. In light of the public health challenges it poses, particularly for children infected with Toxocara canis, the development of effective diagnostic tests or vaccines is of paramount importance. Serological methods are routinely employed to detect specific anti-Toxocara antibodies in infected individuals. This bioinformatics study aims to formulate a multi-epitope protein for Toxocara canis, leveraging various immunoinformatics web servers to enhance the accuracy of serodiagnosis.</p><h3>Methods</h3><p>The identification of linear and conformational B-cell epitopes for the antigens TES-26, TES-30, and TES-120 was conducted using the ABCpred and BepiPred servers. Various web servers were employed to evaluate antigenicity, solubility, and physicochemical properties, as well as to examine secondary and tertiary structures, enhance the three-dimensional model, and confirm the findings.</p><h3>Results</h3><p>This process led to the identification of conformational B-cell epitopes, aimed at exploring possible protein-antibody interactions.</p><h3>Conclusion</h3><p>Ultimately, further experimental investigations are essential for this multi-epitope construct before it can be incorporated into commercial serodiagnosis kits.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 2\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-025-01027-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-025-01027-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景弓形虫病被认为是影响动物和人类最广泛的疾病之一,在全球都有分布。鉴于该病对公共卫生造成的挑战,特别是对感染犬尾丝虫的儿童而言,开发有效的诊断测试或疫苗至关重要。血清学方法是检测感染者体内特异性抗毒原虫抗体的常规方法。这项生物信息学研究旨在利用各种免疫信息学网络服务器,为犬弓形虫配制多表位蛋白,以提高血清诊断的准确性。利用各种网络服务器来评估抗原性、溶解性和理化性质,以及检查二级和三级结构、增强三维模型和确认研究结果。结果这一过程导致了构象 B 细胞表位的鉴定,目的是探索可能的蛋白质-抗体相互作用。
Designing of a Multi-Epitope Antigen for Toxocariasis Diagnosis: An in Silico Approach
Background
Toxoariasis is recognized as one of the most widespread diseases affecting both animals and humans, exhibiting a global distribution. In light of the public health challenges it poses, particularly for children infected with Toxocara canis, the development of effective diagnostic tests or vaccines is of paramount importance. Serological methods are routinely employed to detect specific anti-Toxocara antibodies in infected individuals. This bioinformatics study aims to formulate a multi-epitope protein for Toxocara canis, leveraging various immunoinformatics web servers to enhance the accuracy of serodiagnosis.
Methods
The identification of linear and conformational B-cell epitopes for the antigens TES-26, TES-30, and TES-120 was conducted using the ABCpred and BepiPred servers. Various web servers were employed to evaluate antigenicity, solubility, and physicochemical properties, as well as to examine secondary and tertiary structures, enhance the three-dimensional model, and confirm the findings.
Results
This process led to the identification of conformational B-cell epitopes, aimed at exploring possible protein-antibody interactions.
Conclusion
Ultimately, further experimental investigations are essential for this multi-epitope construct before it can be incorporated into commercial serodiagnosis kits.
期刊介绍:
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.